January 1976
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542 Reads
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25,537 Citations
Analytical Biochemistry
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January 1976
·
542 Reads
·
25,537 Citations
Analytical Biochemistry
... The lysate was centrifuged at 13,000× g for 10 min at 4 °C and the pellet was discarded. Protein concentrations in the supernatant were measured by the Bradford assay [19]. Equal protein samples (30 µg) were solubilized with SDS polyacrylamide gel electrophoresis sample buffer, boiled for 5 min, and electrophoresed through a 12% SDS gel. ...
January 1976
Analytical Biochemistry