M Vilhelmová's research while affiliated with Institute of Molecular Genetics AS CR and other places

Publications (17)

Article
Using Prague recombinant congenic lines of chickens, we found that neonatal thymectomy led either to a marked suppression (CC donors) or to a transient increase (CB donors) of GvH response against the B-F/L + B-G and B-F/L disparate congenic embryos. Similarly, preimmunization with the B (MHC) different blood led either to the suppression or increa...
Article
Evidence for further complexity of the genetic structure of chicken major histocompatibility complex (B) is reviewed, with a historical account showing mutual dependence of the development of an animal genetic model and the growth of scientific knowledge concerning the chicken MHC.
Article
Based on recombinants between the B haplotypes of chickens of inbred lines CB, CC, and CB.I-B7, three new inbred lines homogeneous in transplantation and erythrocyte antigens were established by backcrossing. These newly established recombinant lines, CB.R1, CC.R1, and CC.R2, together with the original inbred lines, which differ at the B complex (M...
Article
Normal mouse sera from non-immunized individuals of different strains gave high titres of "natural" antibodies which agglutinated RBC's of different inbred lines of chickens. Normal mouse sera of A strain and its congenic lines agglutinated RBC's of all chicken lines whereas normal sera of B10 strain and its congenic lines did not react with RBC's...
Article
Among hybrids of the inbred chicken lines, cock 1357 was found to be chimaeric in red blood cells. This cock possessed three serologically distinct types of erythrocytes and two types different in shape and size.
Article
Among hybrids of the inbred lines, a chicken was found to be chimaeric in red blood cells and skin cells and to produce 3 types of sperm cells. This bird could have originated either from the fusion of 2 blastoderms or from the fertilization and fusion of the oocyte and the 2nd polar body.
Article
In a population of (CB X CC)F1 X WB hybrids, a chicken was found with a presumably recombinant haplotype, BR1, whose antigenic products detectable by hemagglutination contained determinants derived from both parental haplotypes, i.e. B1 (from CB) and B2 (from CC). This recombinant bird and its progeny from different crosses were tested by skin graf...
Article
We will present in this paper some new data which contribute to the analysis of the recombinant B-haplo-type in Prague, RI, which was mentioned already in the preceding section of this meeting. In addition, there will be new data concerning the extent of the similarity in B-haplotypes of the inbred CC strain in Prague and the B-complex-homozygous A...
Article
The major histocompatibility complex (MHC) of the chicken, which had been described as a blood group system (Briles et al. 1950), shortly after its discovery attracted attention of the investigators that the genotype of this genetic system may influence viability, hatchability and a number of economically important traits (Gilmour I960, Briles 1964...

Citations

... Simonsen (1982) pointed out that it is an accepted fact that the major histocompatibility complex exerts a dominant role in the functioning of the entire immune system. He commented further that the B locus no longer is considered to be a single locus, but a complex consisting of three regions F, L, and G (Hala et al, 1977). Genes in the F region influence regression, but those in the G region do not (Collins and Briles, 1980;Auclair et al, 1981;Plachy andBenda, 1981). ...
... Murine mAbs recognizing the chicken analogues of the CD3 complex (CT3), CD4(CT4), CD8(CT8), the TCR y/b heterodimer (TCR1), and the TCR-a//3 heterodimer (TCR2) were kindly provided by Dr. M.D. Cooper, Birmingham, AL, and Dr. J. Cihak, Munich, FRG (21)(22)(23)(24)(25). The mAb to the chicken ID2 receptor (I1J2R; code INN-LD16) and alloantibodies against chicken B-L4 or B-L' 2 (MHC Class II) antigens were prepared and characterized in our laboratory (26,27). ...
... At the same time, no antibodies against donor strain lymphocytes were detected using a sensitive GVH-inhibition assay. This assay is known to be especially sensitive for the detection of anti-B-F alloantibodies (8). B-G and B-F antigens are both well expressed on RBC (13). ...
... These systems were discovered by injection of whole blood or blood cells from one chicken to another followed by haemagglutination to detect antibodies. As part of the effort to understand these serologically detected systems, lines of chickens were bred to be homozygous for antigenic alleles of blood group B (Abplanalp et al. 1981;Gilmour 1959), and functional assays emblematic of the MHC, including graft rejection, mixed lymphocyte reaction, graft-versus-host reaction, immune response to limited epitopes and autoimmunity, were found to be determined by the B locus (Bacon et al. 1973;Gebriel and Nordskog 1983;Nordskog 1961, 1963;Vilhelmova et al. 1977). ...
... Compared with the MHC of typical mammals, the BF-BL region of chickens (also sometimes called the 'classical MHC' or the 'core MHC') is compact, simple, and arranged differently ( Figure 1), with two class II B (so-called BLB) genes flanking the tapasin gene located near the DM genes followed by a pair of class I (so-called BF) genes that flank the TAP genes and, finally, the class III region genes [16]. Moreover, no recombination within the BF-BL region has been observed in experiments [19][20][21][22], although comparison of haplotypes shows that there has been some recombination over unknown spans of time [23][24][25]. Also, the genes involved in peptide loading (tapasin, TAP, and DM) are all highly polymorphic, with each BF-BL haplotype generally having a unique set of alleles [24,[26][27][28]. ...
... To obtain long-read sequences from the chicken genome, we used genomic DNA samples from a female inbred line C White Leghorn chicken (28). Genomic DNA was quantified on the Qubit fluorometer (Invitrogen, Carlsbad, CA) and used for sequencing library preparation (SQK-LSK109; Oxford Nanopore Technologies, Oxford, U.K.). ...
... All immunization and tumor induction experiments were carried out with the highly inbred chicken lines CB (B12/ B12), CB.RI (B12r1/B12r1) and CC (B4/B4), CC.RI (B4r1/ B4r1) maintained at the Institute of Molecular Genetics, Prague. Recombinant haplotype B12r1 is composed of B-F12 and B-G4 genes whereas the haplotype B4r1 is reciprocal being composed of B-F4 and B-G12 genes [29]. All chicken lines were free of exogenous avian leukosis viruses. ...