M Trippler's research while affiliated with University of Duisburg-Essen and other places

Publications (28)

Article
The interferon-stimulated gene 15 (ISG15) plays an important role in the pathogenesis of hepatitis C virus (HCV) infection. ISG15-regulated proteins have previously been identified that putatively affect this proviral interaction. The present observational study aimed to elucidate the relation between ISG15 and these host factors during HCV infecti...
Article
HCV RNA assays are of central importance for virological diagnostics and for clinical planning and monitoring of an antiviral combination treatment of chronic HCV infections. The objective of the pre-market evaluation of the VERSANT HCV RNA 1.0 Assay (kPCR) was to collect analytical performance data for this new method of HCV RNA quantification and...
Article
Aims: IFN-α plays an important role in the defense against most viruses and is used for the treatment of hepatotropic viruses (HCV, HBV). However, there is still only little known about the specificity of the IFN-α response in non-parenchymal liver cells and its regulation by anti-inflammatory cytokines. Methods: To address these questions murine...
Article
Aims and background: The mechanism of action of interferon-alpha (IFN-α) which is used therapeutically to suppress replication of the Hepatitis C Virus (HCV) in chronically infected patients is still unclear. Methods: To identify target genes that mediate the anti-HCV effect of IFN-α in vivo 39 HCV patients were treated with a standard therapy (PE...

Citations

... 12 Furthermore, upregulated expression of ISG15 was observed at both the mRNA and protein levels in liver tissue samples from patients with HCV infection compared to the levels detected in uninfected controls. 13 Similarly, the abundance of ISG15 transcripts was found to be increased in human PBMCs and liver cells in patients with HCV infection who were unresponsive to IFN treatment compared to the levels in corresponding IFN-responsive patients. 14 In addition, high levels of ISG15 in the liver of patients with HCV infection were associated with an unfavourable HCV genotype 1, a high hepatic HCV load and a low antiviral response to IFN compared to patients who did not present such characteristics. ...
... Viruses can be also detected using viral genomic DNA and genomic, messenger and micro RNA. 27 Recently, FDA has approved several assays and platforms for detection of genomic and messenger viral RNA, summarized in Table I. 26,[28][29][30][31][32][33][34] These platforms offer a limit of detection (LOD) of 10-200 copies/ml, and linear quantification up to 10 À8 copies/ml. Such new point-of care diagnostic assays capable of achieving comparable or lower limits of detection than current ones would allow better patient management with antiviral therapies and maybe even allow higher cure rates. ...