M. Recacoechea's scientific contributions
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Publications (8)
The present development of planned and unplanned areas of colonization in the primary forest of Bolivia, together with mining, oil prospection and the opening of new roads, etc., has increased considerably the risk of infection by leishmanias. We have established that leishmaniasis is endemic in 5 of the 9 states of Bolivia (Recacoechea, 1983).
Development of new colonization areas is increasing in the tropical Lowlands of Bolivia. They are located easterly on the Amazonian side of the Andes Cordilleras and are covered mainly by primary rain forest. Most of the colonization process is spontaneous with no planning whatsoever. as a consequence of spontaneous migration and settling, no basic...
Within the frame of molecular identification of Leishmania species, karyotyping by Pulsed Field Gradient Gel Electrophoresis (PFG) (Schwartz et al., 1983) has been applied (Spithill and Samaras, 1985; Comeau et al., 1986; Garveyand and Santi, 1986; Giannini et al., 1986; Scholler et al., 1986). In this new kind of electrophoresis, the combination o...
In the context of the LEISHBOL project in Yapacani in the Lowlands of Bolivia (see Recacoechea et al. (Part 1), in this chapter) a parasitological technique for in vivo isolation was evaluated. Identification of the isolates used exploited biological and molecular characteristics, as well as, parasitological and serological information (eg Recacoec...
Citations
... In order to ensure the homogeneity of the parasite population, the strains were cloned by the micro-drop method (Van Meirvenne et al. 1975) and characterized by multi-locus enzyme electrophoresis (13 enzymes, Bañ uls, 1998). Identity of the strains was confirmed by Pulsed Field Gradient Electrophoresis before the beginning of the experiments, as described elsewhere (Dujardin et al. 1987). For each strain, cryostabilates made from parasites with a minimum number of subinoculations were thawed and first cultivated at 26 xC in a biphasic agar medium supplemented with 15 % defibrinated rabbit blood and 0 . ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/788509182943234-1565006647681_Q64/Jean-Claude-Dujardin.jpg)
... In Bolivia, CL is mostly caused by L. (Viannia) braziliensis (up to 85% cases), [16][17][18][19][20][21][22][23][24][25] L. (Leishmania) amazonensis, 24,26 and L. (V.) lainsoni 22,[27][28][29] ; recently, some cases have also been found to be caused by L. (V.) guyanensis ( Table 1 ). 22 All parasite isolates that have been characterized to species have primarily been reported in the north, center, and east of the country (i.e., Departments of La Paz, Beni, Pando, Santa Cruz, and Cochabamba) ( Figure 1 ). ...
