M Bergeret's research while affiliated with Paris Descartes, CPSC and other places

Publications (105)

Article
A novel rapid monoclonal enzyme immunoassay stool antigen for Helicobacter pylori detection (Rapid HpStAR) was evaluated in 16 infected and 92 noninfected children. The overall sensitivity, specificity, and positive and negative predictive values were 87.5%, 97.8%, 87.5%, and 97.8%, respectively, with an accuracy of 96.2%. The negative predictive v...
Article
Knowledge about Helicobacter pylori infection in children continues to advance. While its prevalence appears to be falling in developed countries, it remains a major problem in developing nations. Its transmission pathway remains highly controversial. It has not yet been definitively elucidated, although the oral-oral route seems most probable. Inf...
Article
Full-text available
Analysis of the evolutionary dynamics of Helicobacter pylori allowed tracing the spread of infection through populations on different continents but transmission pathways between individual humans have not been clearly described. To investigate person-to-person transmission, we studied three families each including one child with persistence of sym...
Article
Osteo-articular infections represent the majority of invasive Staphylococcus aureus infections in children. It is demonstrated that, independently of the resistance or susceptibility to methicillin, the production of Panton-Valentine leukocidin (PVL) by S. aureus enhanced the severity of the disease. However, an increase of the rate of community ac...
Article
The yearly prevalence between 1994 and 2005 of primary resistance to amoxicillin, metronidazole, and clarithromycin of 377 Helicobacter pylori strains isolated from children was studied. All the H. pylori strains were susceptible to amoxicillin, 138/377 (36.7%) were resistant to metronidazole, 86/377 (22.8%) to clarithromycin, and 30/377 (7.9%) to...
Article
Osteo-articular infections represent the majority of invasive Staphylococcus aureus infections in children. It is demonstrated that, independently of the resistance or susceptibility to methicillin, the production of Panton-Valentine leukocidin (PVL) by S. aureus enhanced the severity of the disease. However, an increase of the rate of community ac...
Article
The aim of our study was to assess the different mutations involved in clarithromycin-resistant Helicobacter pylori strains isolated from French children and their temporal trends. The point mutations of H. pylori were detected by PCR followed by RFLP technique in 50 clarithromycin-resistant strains collected between 1993 and 2004 in France. Clarit...
Article
The aim of this study was to assess the resistance to metronidazole and clarithromycin of individual colonies of Helicobacter pylori from a single biopsy taken from 14 adults and 14 children. The Etest was used to determine the minimum inhibitory concentrations (MICs) of these two antimicrobial drugs for ten individual H. pylori colonies isolated f...
Article
A novel rapid monoclonal enzyme immunoassay stool antigen for Helicobacter pylori detection (Immunocard STAT!HpSA, Meridian Diagnostic Inc , Cincinnati, OH) was evaluated in 29 infected and 99 noninfected children. The overall sensitivity, specificity, and positive and negative predictive values were 86.2%, 92.9%, 78.1%, and 95.8%, respectively, wi...
Article
Full-text available
To look for evidence of intrafamilial infection, we isolated 107 Helicobacter pylori clones from biopsied specimens taken from both parents and four children. We compared the sequences of two housekeeping genes (hspA and glmM) from these clones with those of 131 unrelated strains from patients living in different geographic regions. Strain relation...
Conference Paper
Background: To demonstrate intrafamilial transmission and mechanisms involved in genome evolution through the molecular study of multiple independent isolates of H. pylori originating from family members. Methods: The family consists of the 2 parents (M, mother, F, father) and 4 siblings (C1 to C4) 14, 12, 8, and 2 years old, respectively, sufferin...
Article
Infections caused by coagulase-negative staphylococci are becoming increasingly important, particularly those of nosocomial origin, as the organisms are frequently multi-resistant. New antimicrobial strategies are needed. The bactericidal activity of a combination of cefpirome with either vancomycin or teicoplanin against 12 strains of methicillin-...
Article
Streptococcus pneumoniae and Haemophilus influenzae are the two main pathogens responsible for bacterial respiratory tract infections. Their antimicrobial susceptibility to antibiotics like beta-lactams, macrolides or fluoroquinolones has been largely studied, while it remains less known to other antibiotics like thiamphenicol, erythromycin, cotrim...
Article
Full-text available
L 'incidence des pneumopathies est d'env i ron 0,7 à 4 épi-sodes pour 100 enfants chez les enfants de moins de 5 ans dans les pays déve l o p p é s , a l o rs qu'elle est de 21 à 29 épisodes pour 100 enfants dans les pays en voie de développement (1). Les traitements antibiotiques ont tra n s fo rmé leur évolution et la guérison sans séquelle est a...
Article
Streptococcus pneumoniae and Haemophilus influenzae are the two main pathogens responsible for bacterial respiratory tract infections. Their antimicrobial susceptibility to antibiotics like β-lactams, macrolides or fluoroquinolones has been largely studied, while it remains less known to other antibiotics like thiamphenicol, erythromycin, cotrimoxa...
Article
To identify pathogenic microorganisms responsible for hospital-acquired bloodstream infections and to evaluate the associated risk factors in pediatric units, in a case-control study over 30 months from January 1st 1997 to June 30th 1999. Forty-six of 855 (5.4%) positive blood cultures were attributed to nosocomial infections. They were related to...
Article
Objectives. – To identify pathogenic microorganisms responsible for hospital-acquired bloodstream infections and to evaluate the associated risk factors in pediatric units, in a case-control study over 30 months from January 1st 1997 to June 30th 1999.Results. – Forty-six of 855 (5.4%) positive blood cultures were attributed to nosocomial infection...
Article
To assess the frequency of acquisition of secondary Helicobacter pylori resistant-strains after a first course of antimicrobial treatment. A retrospective study was performed during the 1994-2000 period, in 15 girls and eight boys, mean age 10.9 +/- 4.8 years (1.4-17 years), with Helicobacter pylori gastritis (culture and antimicrobial susceptibili...
Article
Aims. – To assess the frequency of acquisition of secondary Helicobacter pylori resistant-strains after a first course of antimicrobial treatment.Patients and methods. – A retrospective study was performed during the 1994-2000 period, in 15 girls and eight boys, mean age 10.9 ± 4.8 years (1.4–17 years), with Helicobacter pylori gastritis (culture a...
Article
Since many years, the antimicrobial resistance increases as well as for community-acquired as for nosocomial infections. Antibiotic-resistant pneumococci are neither more nor less virulent susceptible strains. Except for immunocompromised patients, the outcome of penicillin-resistant pneumococcal infections have been similar to those in patients wh...
Article
Since many years, the antimicrobial resistance increases as well as for community-acquired as for nosocomial infections. Antibiotic-resistant pneumococci are neither more nor less virulent susceptible strains. Except for immunocompromised patients, the outcome of penicillin-resistant pneumococcal infections have been similar to those in patients wh...
Article
Full-text available
According to a recent consensus statement concerning Helicobacter pylori infection in children (3), upper gastrointestinal endoscopy with biopsies is the preferred method for establishing an etiologic diagnosis of infection. The treatment recently recommended for children combines a gastric acid inhibitor, usually a proton pump inhibitor (PPI), wit...
Article
Since many years, the antimicrobial resistance increases as well as for community-acquired as for nosocomial infections. Antibiotic-resistant pneumococci are neither more nor less virulent susceptible strains. Except for immunocompromised patients, the outcome of penicillin-resistant pneumococcal infections have been similar to those in patients wh...
Article
Full-text available
Outcome of Helicobacter pylori infection was analyzed in 61 children treated with a triple therapy including clarithromycin. Bacterial eradication was obtained in all children with clarithromycin-susceptible strains but not in children with clarithromycin-resistant ones (P = 0.0001). H. pylori antimicrobial susceptibility is mandatory before choosi...
Article
Full-text available
The aim of the study was to evaluate the prevalence of resistance to amoxicillin, metronidazole, and clarithromycin before treatment ofHelicobacter pylori infection in children and to assess the evolution of resistance with time. The study was carried out between 1994 and 1999 with 150 H. pylori-positive children through gastric culture (antimicrob...
Article
Background. The easiest way to identify the presence of current or past Helicobacter pylori infection is to test for antibodies. The aim of this study was to compare an enzyme-linked immunosorbent assay (ELISA) technique based on the detection of IgG antibodies directed against a global antigenic preparation with immunoblotting based on the analysi...
Article
The bactericidal activity in vitro of cefpirome plus either vancomycin or teicoplanin was compared with that of a cefamandole-vancomycin combination against ten clinical isolates of homogeneous methicillin-resistant Staphylococcus aureus. Cefpirome (0.125 x MIC) combined with vancomycin (0.5-2 x MIC) or teicoplanin (0.5-4 x MIC) acted synergically...
Article
Urinary tract infections (UTI) are the most frequent nosocomial infections in the adult, yet very few data are available concerning these infections in children. In a retrospective 1-year study in a paediatric hospital, we analysed the incidence of nosocomial UTI and the characteristics of the affected children. The incidence was of 1.97/1,000 admi...
Article
The purpose of this study was to evaluate the clinical and histological features of nodular gastritis (NG), its relationships with Helicobacter pylori infection, and its course after H. pylori eradication. Patients and methods: 270 children who underwent outpatient upper gastrointestinal endoscopy at the Saint-Vincent-de-Paul Teaching Hospital in P...
Article
Nosocomial bloodstream infections in pediatrics are an important cause of morbidity and mortality. To identify pathogens causing nosocomial bloodstream infections, evaluate associated risk factors and take preventive measures, we conducted a prospective study from January 1995 to December 1995 at Saint-Vincent-de-Paul Hospital (Paris). All patients...
Article
Background. - Nosocomial bloodstream infections in pediatrics are an important cause of morbidity and mortality. To identify pathogens causing nosocomial bloodstream infections, evaluate associated risk factors and take preventive measures, we conducted a prospective study from January 1995 to December 1995 at Saint-Vincent-de-Paul Hospital (Paris)...
Article
Background. — Nosocomial bloodstream infections in pediatrics are an important cause of morbidity and mortality. To identify pathogens causing nosocomial bloodstream infections, evaluate associated risk factors and take preventive measures, we conducted a prospective study from January 1995 to December 1995 at Saint-Vincent-de-Paul Hospital (Paris)...
Article
In an in-vitro study of the bactericidal activity of cefpirome in combination with vancomycin against 11 clinical isolates of staphylococci, cefpirome at a concentration of 0.25 times the MIC acted synergistically with vancomycin (at a concentration of 0.5, 1 or 2 times the MIC) against Staphylococcus aureus and coagulase-negative staphylococci sus...
Article
The aim of this study was to evaluate the prevalence of H pylori infection in a Parisian children population. During a 3-year period, H pylori infection was investigated in 623 children admitted to our hospital. Children were enrolled into two groups; either a symptomatic children group with clinical gastritis manifestations as infant colics or rec...
Article
Full-text available
A prospective study was performed with 23 Helicobacter pylori-infected children (mean age, 9.5 +/- 4.4 years) with clinical symptoms of gastritis and positive results of culture and histologic examination of gastric biopsy specimens to evaluate the influence of antibiotic resistance on eradication. Positive children were treated for 4 weeks with la...
Article
The aim of this study was to evaluate the prevalence of H pylori infection in a Parisian children population. Patients and methods. - During a 3-year period, H pylori infection was investigated in 623 children admitted to our hospital. Children were enrolled into two groups; either a symptomatic children group with clinical gastritis manifestations...
Article
Background: The purpose of the study was to determine the accuracy of the labelled13 carbon urea breath test for the diagnosis of Helicobacter pylori in children and to simplify the 13carbon urea breath test in identifying the most discriminating sampling time. Methods: H. pylori was searched for in 100 children aged 10.5 ± 4.5 years by histology,...
Article
C57 black mouse splenic T lymphocytes effector cells were co-cultivated with Balb/c mouse splenic cells for sensitization; P815 DBA mouse mastocytoma target cells were then added and specific T cell-dependent cytotoxicity determined. This cytotoxicity increased after gamma-aminobutyric acid (GABA) treatment of the sensitized effectors, but decrease...
Article
Urinary tract infections (UTI) are the most frequent nosocomial infection in the adult, yet very few data are available concerning these infections in children. In a retrospective 1-year study in a paediatric hospital, we analysed the incidence of nosocomial UTI and the characteristics of the affected children. The incidence was of 1.97/1,000 admis...
Article
Objective: To evaluate the efficacy of the amoxicillin-metronidazole combination given with an inhibitor of gastric acid secretion (lansoprazole) in the treatment of Helicobacter pylori infection in mentally-disabled children. Patients and methods: H. pylori infection was diagnosed based on cultures and histologic studies of gastirc specimens obtai...
Article
The classification of serogroup B streptococci in serotype is based on the structural differences of capsular polysaccharides and on presence or absence of a protein c antigen. They are classified as Ia, Ia/c, Ib/c, II, II/c, III, IV and V. The serotype V, unknown in 1970, seems emerging, and is placed in third position of frequency in some America...
Article
Background. — The classification of serogroup B streptococci in serotypes is based on the structural differences of capsular polysaccharides and on presence or absence of a protein c antigen. They are classified as Ia, Ia/c, Ib/c, II, II/c, III, IV and V. The serotype V, unknown in 1970, seems emerging, and is placed in third position of frequency...
Article
A case of staphylococcal scalded skin syndrome in a neonate caused by mother-to-child transmission through breast-feeding is reported. Staphylococcus aureus isolates from mother and child were identified using random-amplified polymorphic DNA analysis, which is a simple and rapid method for comparing strains. This study confirms the need to avoid b...
Article
Antibiotic multi-resistant bacteria are often incriminated in nosocomial infections. To determine the isolation frequency of these bacteria in our laboratory, a descriptive study was carried out during 12 months. Out of 508 Staphylococci aureus strains isolated. 76 (15 %) were methicillin-resistant and essentially found in the trachea (43.4 %) of p...
Article
Full-text available
In an in-vitro study of the bactericidal activity of cefpirome in combination with vancomycin against 11 clinical isolates of staphylococci, cefpirome at a concentration of 0.25 times the MIC acted synergistically with vancomycin (at a concentration of 0.5, 1 or 2 times the MIC) against Staphylococcus aureus and coagulase-negative staphylococci sus...
Article
Careful epidemiological studies and sophisticated diagnostic procedures are necessary to prove that bacterial infection is nosocomial in origin. DNA finger printing method can be useful with this aim in view. A 11 month-old girl suffered from a febrile pneumonia. She developed acute meningitis 15 days later; culture of CSF grew Streptococcus pneumo...
Article
Background. Careful epidemiological studies and sophisticated diagnostic procedures are necessary to prove that bacterial infection is nosocomial in origin. DNA finger printing method can be useful with this aim in view. Case reports. A 11 month-old girl suffered from a febrile pneumonia. She developed acute meningitis 15 days later; culture of CS...
Article
Two genotyping methods were performed on bacterial suspensions of the human pathogen Helicobacter pylori. A total of 29 clinical isolates were analysed by sequencing of a 294-bp PCR-derived internal segment of the essential ureC/glmM gene of H. pylori, and by random amplified polymorphic DNA (RAPD) using a single 11-bp oligonucleotide made up of an...
Article
A serological test for the diagnosis of Helicobacter pylori infection (Cobas Core Roche, IgG, 2nd Generation; Roche, France) was compared with the examination of biopsy samples (culture and histology) obtained after endoscopy in 115 children to assess its value. In 94 children (42 positive and 52 negative), results were concordant. In 10 children a...
Article
Streptococcus milleri is a known commensal of the female genitourinary tract, but its pathogenicity in neonates has been reported in only a few cases. During a period of one year in an obstetrical unit, Streptococcus milleri was isolated from nine neonates and from one foetus after a spontaneous abortion. In seven of the nine newborns, neonatal inf...