Lukasz M. Karbowski’s research while affiliated with Laurentian University and other places

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Publications (52)


Design schematic for in vivo experiments. Male C57 mice received a right flank injection of B16-BL6 cells, expressing large palpable tumors 19 days post-injection (A). Other mice received negative control injections of UV-irradiated B16-BL6 cells (B) or no injections, (C) which did not induce tumor growth.
Photon counts per second from malignant and non-malignant cells show a significant decrease in photon counts (** = p < 0.05) in non-cancerous cells as compared to the cancerous phenotypes. Means and SEMs presented.
(A) Spectral power densities for 0.2 Hz frequency band can accurately classify 83% of 113 cases for cancerous and non-cancerous pancreatic cell lines (** p < 0.001). (B) Discriminant score for cancerous and non-cancerous cell lines. Scores were computed with the following discriminant function (=0.2 Hz Freq * (0.023–1.023).
Raw photon counts per second within the dark-adapted box when no mice were present and when c57 mice were present. Means and standard error or the means are presented (* = p < 0.05).
Photon counts per second by injection group (control = no injection, Tumour = melanoma cells injected, UV = ultraviolet irradiated melanoma cells injected) and time from injection day. Means and SEMs presented. (** = p < 0.05, ns = not significantly different).

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Ultraweak Photon Emissions as a Non-Invasive, Early-Malignancy Detection Tool: An In Vitro and In Vivo Study
  • Article
  • Full-text available

April 2020

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261 Reads

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14 Citations

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Michael A. Persinger

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Lukasz M. Karbowski

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Early detection of cancer improves treatment options and increases survival. Building upon previous demonstrations that ultraweak photon emissions (UPE) could be measured to detect cancers, we designed an early detection protocol to test malignancy in both in vitro and in vivo systems. Photons were measured for 100 s from plates containing ~1 million malignant or non-malignant cells from 13 different types of human and mouse cell lines. Tumor cells displayed increased photon emissions compared to non-malignant cells. Examining the standardized Spectral Power Density (SPD) configurations for flux densities between 0.1 and 25 Hz (Δf = 0.01 Hz) yielded 90% discriminant accuracy. The emission profiles of mice that had been injected with melanoma cells could be differentiated from a non-malignant reference groups as early as 24 h post-injection. The peak SPD associated with photon emissions was ~20 Hz for both malignant cell cultures and mice with growing tumors. These results extend the original suggestion by Takeda and his colleagues (2004) published in this journal concerning the potential diagnostic value of UPEs for assessing proliferations of carcinoma cells. The specificity of the spectral profile in the 20 Hz range may be relevant to the consistent efficacy reported by several authors that weak magnetic field pulsations within this frequency range can diminish the growth of malignant cells in culture and tumor weights in mice.

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Table 1 Complete list of cell lines used in this study and their source. 
Figure 2 of 2
Biophotonic markers of malignancy: Discriminating cancers using wavelength-specific biophotons

November 2017

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844 Reads

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27 Citations

Biochemistry and Biophysics Reports

Early detection is a critically important factor when successfully diagnosing and treating cancer. Whereas contemporary molecular techniques are capable of identifying biomarkers associated with cancer, surgical interventions are required to biopsy tissue. The common imaging alternative, positron-emission tomography (PET), involves the use of nuclear material which poses some risks. Novel, non-invasive techniques to assess the degree to which tissues express malignant properties are now needed. Recent developments in biophoton research have made it possible to discriminate cancerous cells from normal cells both in vitro and in vivo. The current study expands upon a growing body of literature where we classified and characterized malignant and non-malignant cell types according to their biophotonic activity. Using wavelength-exclusion filters, we demonstrate that ratios between infrared and ultraviolet photon emissions differentiate cancer and non-cancer cell types. Further, we identified photon sources associated with three filters (420-nm, 620-nm., and 950-nm) which classified cancer and non-cancer cell types. The temporal increases in biophoton emission within these wavelength bandwidths is shown to be coupled with intrisitic biomolecular events using Cosic's resonant recognition model. Together, the findings suggest that the use of wavelength-exclusion filters in biophotonic measurement can be employed to detect cancer in vitro.









Citations (31)


... These excited molecules emit photons across distinct ranges of the electromagnetic spectrum, from the near-ultraviolet and blue-green regions to the infrared regions ( Figure 3) [83][84][85][86][87]. Studies have shown that malignant cells emit higher levels of UPEs compared to non-malignant cells, which allows differentiation between cancerous and healthy tissues. The spectral power density analysis of UPEs from both human and mouse cell lines was able to distinguish between cancerous and non-cancerous cells with around 90% accuracy [88]. One of the factors in this may be the increased production of ROS by cancer cells [89]. ...

Reference:

Photon-Based Innovations in Oncology: Precise Diagnostic Techniques and Advanced Therapies
Ultraweak Photon Emissions as a Non-Invasive, Early-Malignancy Detection Tool: An In Vitro and In Vivo Study

... It diminishes the growth of only cancer cells without adversely affecting normal cells. Several experimenters [1][2][3][4], employing different equipment and magnetic field patterns, have demonstrated reliable and consistent reduction (~50%) in growth within malignant cell cultures. If this technology is to be employed as a substitute for traditional methods then the variables that interfere with its beneficial effects should be isolated and described before routine treatment begins. ...

Experimental Demonstration That Aharanov-Bohm Phase Shift Voltages In Optical Coupler Circuits of Tuned Patterned Magnetic Fields Is Critical for Inhibition of Malignant Cell Growth

JOURNAL OF ADVANCES IN PHYSICS

... For example, UPE wavelengths were not considered in the current study but were previously found to be predictive of aging, 24,25,27,28 cognitive potential, 29 as well as essential discriminants of healthy and diseased states. 47,48 Our PMTs were calibrated to detect a wide band of UPE wavelengths, which was intentionally inclusive but may have obscured or diluted narrow-band effects. Thus, the use of filters or tunable photodetectors is recommended to determine the wavelength dependencies of brain UPE pattern signatures. ...

Biophotonic markers of malignancy: Discriminating cancers using wavelength-specific biophotons

Biochemistry and Biophysics Reports

... Several theories of learning have been proposed including classical and operant conditioning, social learning theory, and cognitive theory. Classical conditioning which is associated with the Russian physiologist, Ivan Pavlov, is the process in which a neutral or unconditional stimulus becomes a conditioned stimulus after being connected or associated with an unconditioned stimulus [3]. Operant conditioning was made popular by B. F. Skinner in 1937 and he described it as behavior "controlled by its consequences". ...

Experimental Evidence of Classical Conditioning and Microscopic Engrams in an Electroconductive Material

... It was shown in cell cultures that there is a direct correlation between chemical changes in the cell and specific photon emission (64) Ultraweak infrared frequency patterns have been used to differentiate dead and living cells in cell cultures (32) . With the help of the ultraweak photon pattern, malignant cancer cells can be detected,and this finding could become significant for clinical diagnostics (63,64). While mitosis and apoptosis are based on fixed programmes, it is likely that the necessary feedback (66) is provided by physical information systems as described. ...

Ultra-weak Photon Emissions Differentiate Malignant Cells from Non- Malignant Cells In Vitro:
  • Citing Article
  • January 2016

Archives in Cancer Research

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Lukasz M. Karbowski

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... The EMF was applied at 1-3 uT. The software and LED hardware utilized to produce these patterns have been described elsewhere [23,29]. After this 15 min exposure period, the cell plates were taken back to the dark box, where photon measurements were taken again. ...

Synergistic interactions between temporal coupling of complex light and magnetic pulses upon melanoma cell proliferation and planarian regeneration
  • Citing Article
  • July 2016

Electromagnetic Biology and Medicine

... Nonetheless, chemotherapy has an extensive variety of intense and long haul symptoms that significantly influence the patient's nature of life. As it is unrealistic to precisely foresee [11][12][13][14][15] the danger of metastasis advancement in individual patients, these days more than 80% of them get adjuvant chemotherapy, albeit just [15][16][17][18][19][20] roughly 40% of the patients backslide and at last bite the dust of metastatic breast tumour. Along these lines, numerous women who might be cured by nearby treatment alone, which incorporates surgery and radiotherapy, will be over-treated and endure the lethal reactions of [20][21][22][23][24][25][26] chemotherapy unnecessarily. ...

Differentiation of Malignant Compared to Non-Malignant Cells by Their Bio-Photon Emissions May Only Require a Specific Filter around 500 nm
  • Citing Article
  • January 2016

Journal of Cancer Science & Therapy

... For example, exposure to a frequency-modulated EMF called the Thomas-EMF is capable of reducing proliferation of B16-BL6 melanoma cells in vivo and in vitro [31][32][33]. This same patterned EMF (e.g., the Thomas-EMF) was also shown to increase analgesia (reduce nociceptive responses) in rats and affect the growth rates of bisected planaria and cancer cells [28,[34][35][36][37][38][39]. Our lab has also shown that exposure to an EMF based on the firing pattern of amygdaloid neurons (Burst-firing EMF) [40][41][42][43] was capable of inducing analgesia in rats [44], ameliorated self-reported mood and depression scores in human participants [45,46], and activated mu opioid receptors in cancer cells without enhancing their growth [28]. ...

Rotational Frequency Matching of the Energy of the Changing Angular Velocity Magnetic Field Intensity and the Proton Magnetic Moment Produces a Ten Fold Increased Excess Correlation in pH Shifts in Spring Water

NeuroQuantology

... Such durations of measurements of photon emissions over cultures of malignant cells or non-malignant cells have produced similar levels of very accurate discrimination [11]. The presence or absence of a living system such as a mouse within a closed chamber has been verified as well by direct photon measurements [12]. Our unpublished studies indicate that the SPD of the photon emissions from C57 mice that have been injected with mouse melanoma cells allowed reliable and discriminable differences from non-injected reference mice within 48 hr. ...

Cumulative Residual Photon Power Density of ~10-12 W•m-2 During Mild “Distress” in the Same Space: Implications for Temporal Entanglement

NeuroQuantology

... Although the majority of biological functions within the human organism are protected from electromagnetic radiation within observed spectrum by skin and clothes, these artificial lights might still cause some distortions to biological functions due to the lack of full Fig. 10 Diagram of the spectrum a LED lamp (blue), a CFL (green) and an incandescent (purple) superimposed the solar spectrum (yellow) [63] spectrum of sun light on humans. For example, there is the experimental evidence that specific photon energies of weak magnetic field of LED wavelength pulses are stored in malignant cells [64]. ...

Experimental evidence that specific photon energies are “Stored” in malignant cells for an hour: The synergism of weak magnetic Field-LED wavelength pulses

Biologie Médicale