Lucia Borniego's research while affiliated with Indiana University Bloomington and other places

Publications (3)

Article
Full-text available
Previously, we have shown that apoplastic wash fluid purified from Arabidopsis leaves contains small RNAs (sRNAs). To investigate whether these sRNAs are encapsulated inside extracellular vesicles (EVs), we treated EVs isolated from Arabidopsis leaves with the protease trypsin and RNase A, which should degrade RNAs located outside EVs but not those...
Preprint
Full-text available
Previously, we have shown that apoplastic wash fluid purified from Arabidopsis leaves contains small RNAs (sRNAs). To investigate whether these sRNAs are encapsulated inside extracellular vesicles (EVs), we treated EVs isolated from Arabidopsis leaves with the protease trypsin and RNase A, which should degrade RNAs located outside EVs but not those...
Article
The southern green stink bug, Nezara viridula is one of the primary soybean pests and causes significant economic losses around the world. In spite of the high proteases inhibitor (PI) levels, N. viridula can feed on developing seeds of field-grown soybean and reduce crop yields. Although the PI-induced responses have been extensively investigated...

Citations

... They also carried sRNAs that were believed to play an important role in cross-kingdom interactions between Arabidopsis and the fungal pathogen Botrytis cinerea and eventually silenced virulence genes [91]. Remarkably, a recent study suggested that many sRNAs in Arabidopsis leaf EVs are more likely to be associated with proteins outside the membranes instead of being fully encapsulated inside EVs [92]. ...
... These proteins are also attractive targets for genetic analysis, in order to determine how they impact the formation and contents of fungal EVs. Most excitingly, C. higginsianum is a pathogen of the model plant Arabidopsis thaliana, the EVs of which are currently the best-defined among plant species (Baldrich et al., 2019;Karimi et al., 2021;Rutter & Innes, 2017). Front Microbiol, 9, 2286. ...
... Cysteine protease activity was determined on the supernatant as described in previous studies, using the specific substrate p-Glu-Phe-Leu-pNA (Sigma, St Louis, MO, USA; as explained in S7). 42 Membrane lipid peroxidation was analyzed through thiobarbituric acid reactive substances (TBARS) by high-performance liquid chromatography (HPLC) 43 (as explained in the Methods S8). TBARS are formed as a byproduct of lipid peroxidation. ...