Lingyu Liu's research while affiliated with Freie Universität Berlin and other places

Publications (5)

Article
Full-text available
In anti-doping science, the knowledge of drug metabolism is a prerequisite to identify analytical targets for the detection of misused prohibited substances. As the most obvious way to study xenobiotic metabolism, the administration to human volunteers, faces ethical concerns, there is a need for model systems. In the present study, we investigated...
Article
Full-text available
Metandienone and methyltestosterone are orally active anabolic-androgenic steroids with a 17α-methyl structure that are prohibited in sports but are frequently detected in anti-doping analysis. Following the previously reported detection of long-term metabolites with a 17ξ-hydroxymethyl-17ξ-methyl-18-nor-5ξ-androst-13-en-3ξ-ol structure in the chlo...
Preprint
Full-text available
Metandienone and methyltestosterone are orally available anabolic-androgenic steroids with a 17α-methyl structure that are prohibited in sports but are frequently detected in anti-doping analysis. After the previously reported detection of long-term metabolites with a 17ξ-hydroxymethyl-17ξ-methyl-18-nor-5ξ-androst-13-en-3ξ-ol structure in the chlor...
Article
Full-text available
Background Recently the expression patterns of several cytochrome P450 (CYP) genes in different human osteoblast models were reported. However, the various expression patterns of CYPs in human osteoblasts during different stages of osteogenic differentiation have not been investigated and the effect of inflammatory cytokines on CYPs expression in o...
Article
Full-text available
Cytochromes P450 (CYPs) are important for bone homeostasis, but only limited information is available on their expression in human bone cells. We analyzed the expression levels of eight CYPs in osteoblasts cultured in human bone pieces, in osteoblasts differentiated from human periosteum mesenchymal stem cells, in primary human osteoblasts, and in...

Citations

... This study also demonstrated the importance of mass spectrometry. MS is the current technology of choice for detecting and quantifying proteins and metabolites, as it is adaptable to virtually any type of sample, ranging from cells and neurons to subcellular organelles, tissues, and even whole embryos [58][59][60][61][62][63][64]. Mass spectrometers can directly detect intact proteins, peptides, post-translational modifications, and metabolites with high specificity, usually sub-mDa (sub-ppm) mass accuracy, and the capability for both discovery (untargeted) and targeted studies. ...
... Although its clinical approval was withdrawn in the early 1980s, metandienone is still marketed and used for performance enhancement. The metabolism of the orally bioavailable metandienone has been investigated thoroughly for decades, and there is still interest in finding new metabolites which might contribute to an elongation of the detection window (MacDonald et al. 1971;Schänzer et al. 1991Schänzer et al. , 2006Pozo et al. 2009a;Loke et al. 2021;Parr et al. 2012). The most obvious way to study human xenobiotic metabolism is the administration to volunteers and the collection of their urine. ...
... Cyp2e1 codes for a cytochrome P450 enzyme that metabolizes many substrates and is involved in the generation of reactive oxygen species [14]. The functional importance of Cyp2e1 in the bone is unclear, but prior studies have shown that Cyp2e1 expression in osteoblasts decreases with unloading and increases with differentiation as well as after exposure to tumor necrosis factor alpha [15,16]. Systemically increased tumor necrosis alpha levels have been reported in oim mice [17]. ...
... As a target gene of lncRNA SNHG5, hemoglobin subunit beta (HBB) may be involved in the development of male osteoporosis (59). Cytochromes P450 (CYPs) are important for bone homeostasis (60), and cytochrome P450 family 17 subfamily A member 1(CYP17A1) is thought to be a susceptibility loci for altered BMD in PMOP patients (61). In previous studies, there was no direct evidence for the role of other genes, such as VWF, TFPI2, ASNS, and DAPK1 in PMOP. ...