Lin Zhang’s research while affiliated with Okinawa Institute of Science and Technology Graduate University and other places

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Publications (2)


Advantageous Culture Traits of Several Cephalopod Models. Comparison of cephalopod species previously used in laboratory experiments. “Lifecycle closed” refers to a species being cultured across at least one generation. An animal is considered capable of group rearing if minimal aggression and cannibalism is observed, and the stress of group rearing prevents successful culturing efforts. “Multiple spawner” indicates normal multiple spawning events completed by one female. “Precocious offspring” refers to hatchling behaviors similar to adults (including predation). “Small at maturity” refers to an animal with a dorsal mantle length less than 6 cm. Some cephalopod species have evolved a light organ that is bioluminescent. The tree is based on results published by Anderson and Lindgren (2020).
E berryi and E morsei at different life stages. (A) Egg clutches of E berryi (left) and E morsei (right). Scale bar is 5 cm. (B) E berryi at one day post- hatching (dph). Scale bar is 1 mm. (C) E morsei at one dph. Scale bar is 1mm. (D) Mature E berryi at 130 dph. Scale bar is 1 cm. (E) Mature E morsei at 70 dph. Scale bar is 1 cm. (F) E berryi mating. The male (on the right) grasps the female from the ventral side to engage mating. (G) E morsei mating. The smaller male on the right grasps the female from the ventral side to engage mating.
Survivorship, growth rate, and developmental timelines for E berryi and E morsei. (A) Survivorship for each species. For each species, initial population size was 30 individuals. The asterisk (*) represents an artificial reduction in total population size for E berryi from 30 to 19 individuals due to limited tank space. Arrows indicate the first spawning event for each species. (B) Growth rate comparing wet weight (g) to dph on semi-log scale. (C) Growth rate comparing dorsal mantle length (mm) to age on semi-log scale. (D) Comparison of the lifecycle and time between developmental landmarks of both E berryi and E morsei.
Comparison of life cycle and culture traits of cultured Euprymna spp.
Lifecycle, culture, and maintenance of the emerging cephalopod models Euprymna berryi and Euprymna morsei
  • Article
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December 2022

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311 Reads

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11 Citations

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Yuko Hasegawa

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Daniel Rokhsar

Cephalopod research remains limited by the inability to culture species under laboratory conditions for multiple generations to provide continuous access to animals at all stages of the life cycle. Here, we describe a multi-generational laboratory culture system for two emerging cephalopod models: the hummingbird or Berry’s bobtail squid, Euprymna berryi Sasaki, 1929, and Morse’s bobtail squid, Euprymna morsei Verrill, 1881, which are primarily found off mainland Japan. E. berryi wild adults were spawned and raised to the third filial generation, and E. morsei wild adults were spawned and raised to the second filial generation in a closed system at 20°C. We report growth and survivorship data for a cohort of 30 individuals across the first generation raised in captivity. E. berryi and E. morsei grew exponentially during the first 90 and 60 days post-hatching, respectively. Survivorship at the first spawning event for E. berryi and E. morsei was 90% and 77%. E. berryi and E. morsei females spawned after days 112 and 71 days post-hatching, respectively. We describe the life history of each species and how to distinguish sexes. We discuss the challenges of cephalopod culture and how culturing these species address those problems.

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A single-cell atlas of bobtail squid visual and nervous system highlights molecular principles of convergent evolution

May 2022

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218 Reads

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18 Citations

Although the camera-type eyes of cephalopods and vertebrates are a canonical example of convergent morphological evolution, the cellular and molecular mechanisms underlying this convergence remain obscure. We used genomics and single cell transcriptomics to study these mechanisms in the visual system of the bobtail squid Euprymna berryi , an emerging cephalopod model. Analysis of 98,537 cellular transcriptomes from the squid visual and nervous system identified dozens of cell types that cannot be placed in simple correspondence with those of vertebrate or fly visual systems, as proposed by Ramón y Cajal and J.Z. Young. Instead, we find an unexpected diversity of neural types, dominated by dopamine, and previously uncharacterized glial cells. Surprisingly, we observe changes in cell populations and neurotransmitter usage during maturation and growth of the visual systems from hatchling to adult. Together these genomic and cellular findings shed new light on the parallel evolution of visual system complexity in cephalopods and vertebrates.

Citations (2)


... The Euprymna berryi (E. berryi) bobtail hummingbird squid is an emerging new model organism amenable to genetic manipulation 3,4 . Bobtail squids have primarily been studied for their unique symbiotic relationships with bioluminescent bacteria Vibrio fischeri 5 and likely use extensive A-to-I RNA editing to sense and adapt to their environment, similar to other cephalopod species 6 . ...

Reference:

Squid primary cell culture as a model system and experimental tool
Lifecycle, culture, and maintenance of the emerging cephalopod models Euprymna berryi and Euprymna morsei

... We did not find expression of canonical glial markers such as Gfap in vertebrates (Eng, 1985;Eng et al., 2000) or Repo in arthropods (Halter et al., 1995;Xiong et al., 1994) in the Berghia reference transcriptome, and therefore, they were also absent from the scRNAseq data. This is consistent with recent scRNAseq in the brains of cephalopods molluscs (Gavriouchkina et al., 2022;Songco-Casey et al., 2022;Styfhals et al., 2022). ...

A single-cell atlas of bobtail squid visual and nervous system highlights molecular principles of convergent evolution
  • Citing Preprint
  • May 2022