Lianzhong Ai’s research while affiliated with University of Shanghai for Science and Technology and other places

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Publications (312)


LytR Family Transcriptional Regulator EpsA Positively Regulates Exopolysaccharide Biosynthesis in Lacticaseibacillus casei
  • Article

April 2025

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3 Reads

Journal of Agricultural and Food Chemistry

Yizhou Fan

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Xintian Du

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[...]

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Lianzhong Ai




PRISMA flow diagram of the included and excluded articles.
Meta-analysis of ENI incidence in patients with acute pancreatitis. Abbreviations: DL: DerSimonian and Laird; RR, rate ratio; CI, confidence interval [19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46].
Characteristics of the studies included in the systematic review.
Subgroup analysis of ENI incidence in patients.
Results of the meta-regression analysis.

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Clinical Predictors and Prevalence of Enteral Nutrition Intolerance in Acute Pancreatitis: An Updated Systematic Review and Meta-Analysis
  • Literature Review
  • Full-text available

March 2025

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30 Reads

Background: Acute pancreatitis (AP) leads to severe inflammation and nutritional deficits, with 80% of severe cases experiencing critical protein loss. Timely enteral nutrition is essential for recovery. This study systematically reviews and analyzes the incidence and predictors of enteral nutrition intolerance (ENI) in AP patients. Methods: Web of Science, Embase, Cochrane Library, and PubMed were searched up to May 2024. Studies reporting on ENI incidence and predictors in AP patients were included based on predefined criteria. Bias was assessed using standardized tools, and meta-analyses provided summary estimates with confidence intervals. Results: From the 2697 screened studies, 28 involving 4853 patients met the inclusion criteria. The pooled incidence of ENI was 26%. Significant predictors included comorbid diabetes, pancreatic necrosis, elevated pre-refeeding serum lipase levels, peri-pancreatic fluid collections, and systemic inflammatory response syndrome at admission. Higher ENI rates were observed in Europe, among patients with severe acute pancreatitis (SAP), those receiving nasoenteric feeding, and in prospective study cohorts. Conclusions: ENI affects approximately one-quarter of AP patients and is not significantly associated with age, sex, or the cause of AP. Its incidence varies by region, disease severity, feeding method and study design. Identifying predictors, such as comorbid diabetes and pancreatic necrosis, may help clinicians reduce the risk of ENI. The limitations of this study include the heterogeneity of the included studies and inconsistent ENI diagnostic criteria.

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EPS biosynthesis in S. thermophilus. LacS, lactose permease; LacZ, β‐galactosidase; Glk, glucokinase; Pgm, phosphoglucomutase; RmlA, glucose‐1‐phosphate thymidylyltransferase; GalU, UTP‐glucose‐1‐phosphate uridylyltransferase; GalM, aldose 1‐epimerase; GalK, galactokinase; GalT, galactose‐1‐phosphate uridyltransferase; GalE, UDP‐galactose‐4‐epimerase.
Effects of overexpressing precursor gene on cell growth and EPS production. The mean ± SD values were from three independent biological replicates. Significant differences were determined by two‐tailed t‐tests (*P < 0.05).
Effects of co‐overexpressing precursor genes on cell growth and EPS production. The mean ± SD values were from three independent biological replicates. Significant differences were determined by two‐tailed t‐tests (*P < 0.05).
Effects of multigene co‐overexpression on cell growth and EPS production. The mean ± SD values were from three independent biological replicates. Significant differences were determined by two‐tailed t‐tests (*P < 0.05).
The transcriptional change of strain SJK19 grown at 37°C in LM17 medium with 5 μg mL⁻¹ erythromycin. Samples were collected at 24 h of fermentation. (A) The transcriptional change of precursor biosynthesis genes. (B) The transcriptional change of eps gene cluster. The mean ± SD values were from three independent biological replicates.
Engineering UDP‐sugar synthesis pathways for exopolysaccharide biosynthesis in Streptococcus thermophilus AR333

BACKGROUND Exopolysaccharide (EPS) produced by Streptococcus thermophilus can significantly improve the viscosity, texture and taste of dairy products, which have broad application potential in the food industry. However, EPS production is typically low (< 100 mg L⁻¹) in S. thermophilus, making it difficult to meet industrialization requirements. The utilization of metabolic engineering for genetic modification of S. thermophilus is an efficient approach to enhance EPS biosynthesis. RESULTS To our knowledge, there is lack of systematic investigation on engineering UDP‐sugar synthetic pathways for EPS production in S. thermophilus. In the present study, the biosynthetic genes of EPS precursors UDP‐glucose and UDP‐galactose were regulated for improving EPS production in S. thermophilus AR333. Compared with the control, engineered strains by single overexpression of eight EPS precursor genes increased EPS production by 7–31%, respectively. Among of them, overexpressing glk encoding glucokinase and galE1 encoding UDP‐galactose‐4‐epimase led to 275.37 and 288.65 mg L⁻¹ of EPS production, respectively. Moreover, co‐overexpression of lacZ encoding β‐galactosidase and galE1 achieved a remarkable 49% increase in the EPS production (329.51 mg L⁻¹). Transcriptional analysis further suggested that enhanced EPS synthesis in engineered strain can attributed to the upregulation of precursor genes and clusters of EPS genes. CONCLUSION Our results showed that engineered UDP‐sugar synthesis is an efficient strategy to boost EPS production in S. thermophilus. © 2025 Society of Chemical Industry.




The bsh1 gene of Lactobacillus plantarum AR113 ameliorates liver injury in colitis mice

February 2025

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10 Reads

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1 Citation

npj Science of Food

The Western diet (WD) leads to hepatic lipid metabolism disorders. Previous studies have shown that the bile salt hydrolase 1 (bsh1) gene of Lactobacillus plantarum AR113 attenuates colitis under WD. In this study, we preliminarily explored how AR113 attenuates the hepatic inflammatory response in colitis mice on the WD. Our study suggests that the WD leads to abnormalities in hepatic lipid metabolism and dysbiosis of the gut microflora, and furthermore, there may be a correlation between abnormalities in lipid metabolism and hepatic inflammatory responses. AR113 significantly regulated lipid and bile acid metabolism in the liver of mice treated by WD and Dextran sulfate sodium (DSS), affected the structure of the mouse intestinal flora, and inhibited the expression of Sterol regulatory element-binding protein 1C (SREBP-1C) and P-NF-κB p65 at the protein level, thereby attenuating the hepatic injury phenotype. However, the bsh1 knockout strain did not exhibit the above function.



Citations (58)


... Notably, the relative abundance of Ruminiclostridium_6 demonstrated a positive correlation with lymphosin-α and IL-17, and a negative correlation with BW change, suggesting its potential as an indicator of colitis progression [36]. Our results also identified a strong positive correlation between Escherichia_Shigella and pro-inflammatory cytokines IL-17 and IFN-γ, which aligns with previous studies highlighting the pathogenic nature of Escherichia_Shigella in colitic mice [37]. The reduction in the relative abundance of Escherichia_Shigella and downregulation of pro-inflammatory cytokines following L. plantarum treatment underscores its therapeutic potential, which is consistent with other studies demonstrating the beneficial effects of L. plantarum in decreasing gut pathogenic bacteria and mitigating inflammatory responses [38,39]. ...

Reference:

Lactobacillus plantarum 17-1 Ameliorates DSS-Induced Colitis by Modulating the Colonic Microbiota Composition and Metabolome in Mice
The bsh1 gene of Lactobacillus plantarum AR113 ameliorates liver injury in colitis mice

npj Science of Food

... Specific bacterial genera have been identified to influence bone cell activities, particularly osteoclastogenesis (the formation of osteoclasts) and osteoblast activity (bone-forming cells) [8,[54][55][56]. For instance, Lactobacillus strains have been shown to inhibit osteoclast differentiation by reducing pro-inflammatory cytokines such as tumour necrosis factor-alpha (TNF-α) and interleukin (IL)-1β, which are critical for osteoclast formation [57,58]. Additionally, Limosilactobacillus reuteri ( L. reuteri) maintains the Wnt signaling pathway, essential for osteoblast proliferation and differentiation, thereby promoting bone formation [59,60]. ...

Evaluation of Functional Components of Lactobacillus plantarum AR495 on Ovariectomy-Induced Osteoporosis in Mice And RAW264.7 Cells

... Many recent studies have confirmed the ability of lactic acid bacteria, especially the genera Bifidobacterium and Lactobacillus, to break down oxalates to format and CO 2 [5,6]. Qin et al. [7] reported isolating four [8] indicated that Lb. acidophilus bacteria degraded oxalates in industrially prepared gastric fluid by 48%. The present investigation aimed to identify species of lactic acid bacteria from human sources and fermented foods and study their ability to degrade oxalates in vetro ...

Screening of oxalate-degrading probiotics and preventive effect of Lactiplantibacillus plantarum AR1089 on kidney stones
  • Citing Article
  • September 2024

Food & Function

... Common findings include mucosal erythema and ulcerations limited to the rectum and sigmoid colon. Histopathological examination confirms ulcerative colitis through crypt abscesses and mucosal inflammation [29][30][31] . Chronic cases often show basal plasmacytosis and fibrosis. ...

Protection against DSS-induced colitis in mice through FcεRIα deficiency: the role of altered Lactobacillus

npj Biofilms and Microbiomes

... Probiotics may improve the health of the microbiota in the gut, make insulin work better, and reduce inflammation. All of these effects may lead to better metabolic outcomes in people with T2DM [20,21]. ...

Probiotics intervention for type 2 diabetes mellitus therapy: a review from proposed mechanisms to future prospects
  • Citing Article
  • August 2024

... Utilizing biomass to harness its inherent charactertics, biosorption has been emerged as an e cient and sustainable biological method for remedying heavy metals (HMs) contamination in wastewater 24,25 . ...

Probiotic bacterial adsorption coupled with CRISPR/Cas12a system for mercury (II) ions detection
  • Citing Article
  • August 2024

Biosensors and Bioelectronics

... Smart sensors and Internet of Things devices are driving this paradigm shift, enabling real-time tracking and data collection throughout the food production and distribution process. Conventional detection methods, such as atomic absorption spectroscopy (AAS), high-performance liquid chromatography (HPLC), and gas chromatography-mass spectrometry (GC-MS), are hindered by extensive sample preparation, high costs, and lengthy detection times, limiting their broad applications (Abid et al. 2024;Gao et al. 2024;G. Jiang et al. 2024). ...

Research progress on detection of foodborne pathogens: The more rapid and accurate answer to food safety
  • Citing Article
  • July 2024

Food Research International

... Low albumin levels may increase the risk of infection and systemic inflammation, potentially leading to organ dysfunction in patients with decompensated cirrhosis. Additionally, hypoalbuminemia can impair vascular endothelial cell function and increase capillary permeability, further contributing to the risk of UGIB [39]. Additionally, elevated total bilirubin (TBIL) exacerbates hepatic dysfunction, aggravates portal hypertension, and impairs coagulation, all of which contribute to UGIB and increase the risk of variceal rupture and mucosal injury [40]. ...

Editorial: Effects and mechanisms of probiotics, prebiotics, synbiotics and postbiotics on intestinal health and disease

... Figure 4 determine their technological applications. For example, xyloglucan obtained by soaking seeds as a decorticate method can be used in pharmaceutical products with low viscosity (ophthalmological and nasal formulations) [8,69], as well as a thickening agent in juices and nectars [70]. On the other hand, xyloglucan from roasted seeds is adequate in film and coating development and as an excipient in gel formulation [71,72]. ...

Tamarind seeds polysaccharide: Structure, properties, health benefits, modification and food applications
  • Citing Article
  • May 2024

Food Hydrocolloids

... In addition, the suppression of α-glucosidase activities by polyphenols is primarily due to the establishment of hydrogen bonds and hydrophobic interactions between these enzymes and the polyphenolic compounds [23]. The number of hydrogen bonds is pivotal in inhibiting the catalytic ability of the enzyme [46]. As shown in Figure 4, at the active site of α-glucosidase, 3,4-diCQA formed seven hydrogen bonds with amino acid residues Ser241, Asp242, Gln279, His280, Asp307, and Asp352 (Figure 4(A4)); 3,5-diCQA, rutin, and 4,5-diCQA formed three hydrogen bonds with amino acid residues (Figure 4(B4-D4)); and 3-CQA formed nine hydrogen bonds with amino acid residues Asn259, Thr274, Thr290, His295, Glu296, Ser298, and Asp341 (Figure 4(E4)). ...

Inhibitory activities and mechanisms of free and bound phenolics on α-glucosidase in fresh fruits of Phyllanthus emblica Linn. using spectroscopy and molecular docking
  • Citing Article
  • May 2024

Food & Function