Lianqi Yan's research while affiliated with Central South University and other places

Publications (53)

Article
Full-text available
Introduction Kashin-Beck Disease (KBD) is an endemic disease predominantly affecting joint and skeletal muscle, predisposing the articular cartilage to degeneration and necrosis. Currently,staged total hip arthroplasty is a common surgical method for advanced femoral head necrosis from KBD, but there are no reports in the literature on simultaneous...
Article
Full-text available
Osteoarthritis (OA) has been reported as a progressive disease in the elderly, primarily characterized by degenerated articular cartilage. There has been no satisfactory drug for the treatment of OA. DL-3-n-butylphthalide (NBP), a small molecule compound extracted from celery seeds, may have antiapoptotic, antioxidant, and anti-inflammatory activit...
Article
Background: No consensus has been reached for the treatment of vertical femoral neck fractures (vFNFs). Recently, two alternative methods were invented to treat vFNFs, one of which is a new plate with a sliding groove, which was designed as a substitution of the medial buttress locking plate to combine with cannulated compression screws (CCS) for r...
Article
Epidural fibrosis after lumbar laminectomy refers to a serious complication, and excessive proliferation of fibroblasts is considered the major factor. Interferon-alpha-2b (IFN-α-2b) can exert antiviral and antiproliferative effects, which has been suggested to effectively prevent several fibrotic diseases. However, the effect of IFN-α-2b on the pr...
Article
Objectives: To explore the risk factors for femoral neck fracture in elderly population. Methods: A total of 124 elderly patients (≥60 years old) in hospital for trauma were enrolled, including 71 patients (57%) with femoral neck fracture and 53 non-femoral neck fracture patients (43%). All patients' age, gender, body mass index (BMI), bone mine...
Article
Post-surgery arthrofibrosis is one of the most restrictive factors in the development of intra-articular surgery and has presented tremendous obstacles for most orthopaedic surgeons. Tanshinone IIA (Tan IIA), a key active ingredient of Den-shen, has been used to treat fibrosis-related diseases, such as pulmonary, hepatic and myocardial fibrosis. In...
Article
Full-text available
Purpose Femoral neck fractures are still unsolved problems nowadays; sliding hip screw (SHS) and cannulated compression screw (CCS) are the most commonly used devices. We evaluated the clinical outcomes and complications in the treatment of femoral neck fractures between SHS and CCS in this meta-analysis to find which is better. Methods We searche...
Article
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A stem cell-based tissue-engineering approach is a promising strategy for treatment of cartilage defects. However, there are conflicting data in the feasibility of using this approach in young recipients. A young rabbit model with an average age of 7.7 months old was used to evaluate the effect of a tissue-engineering approach on the treatment of o...
Article
Aim: To compare the efficacy of 3D printing-assisted surgery with routine surgery in the treatment of distal radius fractures to evaluate whether 3D printing technology has more advantages. Materials & methods: To retrieve all published studies that compared the efficacy of 3D printing-assisted surgery with routine surgery for distal radius fractur...
Article
Objective: To investigate the effects of everolimus (EVE) on postoperative fibrosis in the knee joint and the potentially relevant signaling pathways. Methods: CCK-8 and flow cytometry assays were used to detect the effect of EVE on human fibroblast viability and apoptosis induction. IF and TEM were used to assess fibroblast autophagy. 3-methyla...
Article
Aim Pathologic hyperplasia of fibroblast is responsible for the progression of intraarticular fibrosis. Laminin α4 (LAMA4), a subunit of laminin macromolecule family, was found to be overexpressed in various fibrotic tissues. However, the role of LAMA4 in knee arthrofibrosis remains elusive. Therefore, the aim of this study was to investigate the e...
Preprint
Full-text available
Background Excessive fibroblasts proliferation is believed as a major reason in the process of epidural fibrosis, which is known as a troublesome complication of lumbar laminectomy clinically. Mycophenolate mofetil (MMF) is a kind of immunosuppressant, previous studies had shown that it has the function of preventing fibrosis, but the role and mech...
Article
Full-text available
Background We investigated the role of decellularized cartilage matrix in osteoarthritis to seek a new treatment for this disease. Methods Knee cartilage from rabbits was decellularized and the degree of decellularization was assessed. A grinder was used to turn acellular cartilage into particles, which were then used in a suspension. Thirty New Z...
Preprint
Bankground: As one of the hot cells in the field of regenerative medicine, adipose mesenchymal stem cells(ADSCs) have been proved to have the ability of myoblast differentiation, but the disadvantage is that the efficiency of myoblast differentiation is not very high.Extracellular matrix(ECM), as a mixture of cytokines and proteins secreted by cell...
Article
Lumbar laminectomy is commonly deemed as the most valid surgery for a series of lumbar illnesses, such as lumbar disc herniation, which could lead to spinal canal stenosis. However, epidural fibrosis is one of the most common complications that limits the application of lumbar laminectomy, which is mainly caused by proliferation of local fibroblast...
Article
Full-text available
In the last decade, researchers have searched for predictive surface markers of multipotent mesenchymal stromal/stem cells (MSCs) for ensuring improved therapeutic outcomes following cartilage damage in humans. However, we have achieved only limited progress because of the challenge presented by conflicting data. This commentary provides some evide...
Article
Full-text available
Background: Intraarticular scar adhesion refers to a serious complication caused by knee surgery or trauma, leading to various sequelae (e.g., articular cartilage degeneration and knee joint stiffness). Artesunate (ART) has exhibited an effect to suppress fibroblast proliferation, whereas the exact mechanism remains unclear. This study aims to del...
Article
Fibroblasts excessive proliferation was considered as a decisive reason for epidural fibrosis, which was known as a serious complication of lumbar laminectomy. As a traditional Chinese medicine, triptolide (TP) was used to be proved effective in preventing several fibrosis scar formation diseases. However, little is known about the effect of TP on...
Article
Full-text available
Background: Failed back surgery syndrome (FBSS) is a common complication after the laminectomy. Epidural fibrosis is the major cause of lower back pain and other complications. Numerous studies have shown that apigenin (API) could treat various fibrotic diseases by regulating various signaling pathways, whereas no study has discussed whether API c...
Article
Intraarticular fibrosis following knee surgery is a troublesome complication and remains a challenging problem for clinicians. Artesunate (ART), a classical anti-malarial drug extracted from the Chinese medicinal herb Artemisia annua L, has been associated with some fibrosis-related diseases. However, its effect and underlying mechanism on knee art...
Article
Post‐operative skeletal fibrosis is considered one of the major complications causing dysfunction of the skeletal system and compromising the outcomes of clinical treatment. Limited success has been achieved using current therapies; more effective therapies to reduce post‐operative skeletal fibrosis are needed. Stem cells possess the ability to rep...
Article
Full-text available
10-Hydroxycamptothecin (HCPT) effectively controls epidural fibrosis, but the exact underlying mechanisms remain ambiguous. Abnormal microRNA (miR)‑23b‑3p expression has been detected in various types of fibrotic tissues that are present in different diseases. The aim of the present study was to elucidate the mechanisms through which HCPT induces f...
Article
Fibroblast proliferation is considered to be a major cause in the process of epidural fibrosis formation. Autophagy is a tightly-regulated catabolic process in charge of degrading intracellular components. Although autophagy has been associated with fibrosis of different tissues, the effect of autophagy on epidural fibrosis is still unknown. The ai...
Article
Full-text available
Rationale: Microcystic/reticular schwannoma (MRS) is a rare histological variant of schwannoma which was initially described in 2008 with a predilection for the visceral organs. This distinct tumor had been reported to mainly affect gastrointestinal tract, subcutaneous and soft tissue, various glands and head and neck region. However, MRS involvin...
Article
Statement of significance: The use of decellularized extracellular matrix (dECM) is becoming a promising approach for tissue engineering and regeneration. Compared to dECM derived from cartilage tissue, recently reported dECM from cell sources exhibits a distinct role in cell based cartilage regeneration. In this review paper, for the first time,...
Article
Many reasons contribute to epidural fibrosis after lumbar laminectomy and the excessive proliferation of fibroblast is an important factor. Tamoxifen (TAM)has been confirmed that can inhibit fibroblast proliferation and reducefibros is in many studies, but the detailed effect and mechanism of TAM on preventing epidural fibrosis are unknown. In orde...
Article
Wear debris-induced osteolysis is one of the major reasons for subsequent aseptic loosening after cementless hip arthroplasty. Increasing evidence suggests that receptor activator of nuclear factor kappa-B (NF-κB) ligand-mediated osteoclastogenesis and osteolysis are responsible for wear debris-induced aseptic loosening. In the present study, we ex...
Article
Fibroblast excessive proliferation is considered as one of the major reasons of epidural fibrosis after laminectomy. Recent studies have shown that mitomycin C (MMC) could successfully reduce the formation of epidural fibrosis by inducing fibroblasts apoptosis. However, the detailed mechanism was still unclear. Increasing evidence indicated that P2...
Article
Surgery-induced epidural fibrosis after laminectomy often results in poor clinical outcomes. Fibroblasts proliferation is considered to be one of the major causes of epidural fibrosis formation. Previously, there was no research about the effect of Homoharringtonine(HHT) on inhibiting fibroblast proliferation and reducing epidural fibrosis. Here, w...
Article
Intraarticular adhesion is one of the complications when the patients suffer from operations of knee-joint. Hydroxycamptothecin (HCPT) can selectively inhibit the activity of topoisomerase I and has shown anti-proliferative effect in previous researches. The aim of our experiment was to investigate the effect of topical application of HCPT on preve...
Article
Tacrolimus (FK506) has been demonstrated to reduce epidural fibrosis. However, the detailed mechanism of action has not been elucidated. Aberrant miR-429 is involved in many diseases. The aim of this study was to describe the exact mechanism of FK506 induced apoptosis in fibroblasts and the prevention of epidural fibrosis. FK506 induced fibroblast...
Article
Aseptic periprosthetic osteolysis is a complication induced by wear particles after hip joint replacement, which shortens the service life of prostheses. Although many methods have been applied to prevent this complication, the results were not always successful. In the present study, we explored the prophylactic effect of notoginsenoside R1 (NG-R1...
Article
Full-text available
The fibrosis that develops following laminectomy or discectomy often causes serious complications, and the proliferation of fibroblasts is thought to be the major cause of epidural fibrosis. 10-Hydroxycamptothecin (HCPT) has been proven to be efficient in preventing epidural fibrosis, but the exact mechanism is still unclear. NOXA is a significant...
Data
The raw data of CCK-8 assay, Tunel assay, H&E staining and immunohistochemistry staining Full-length uncropped western blots were contained in this file, the PVDF membranes were cut into pieces before exposure.
Article
Lumbar laminectomy is one of the most common treatments for lumbar disc herniation and other lumbar disorders with serious complications, such as failed back surgery syndrome, mainly caused by epidural fibrosis (EF). The developing fibrosis causes radicular pain after the laminectomy or discectomy. Methotrexate (MTX) is a folic acid antagonist that...
Article
Aseptic prosthetic loosening is a major complication after hip joint replacement. Wear particle-induced periprosthetic osteolysis plays a key role in aseptic prosthetic loosening. Attempting to modulate receptor activator of nuclear factor-κB (RANKL) mediated signaling pathways is a promising strategy to prevent aseptic prosthetic loosening. In the...
Article
Full-text available
Background Epidural fibrosis often causes serious complications in patients after lumbar laminectomy and discectomy and is associated with the proliferation of fibroblasts. Suramin is known to have an obvious inhibitory effect on the coactions of many growth factors and their receptors, but little was previously known about the effect of suramin on...
Article
Full-text available
Background: Epidural fibrosis is a common complication after laminectomy. It is associated with intractable lower back pain and additional complications. To date, no study has evaluated whether the local application of rapamycin (RAPA) can inhibit fibroblast proliferation and reduce epidural scar adhesion after laminectomy. The results of the pres...
Article
Full-text available
Background The formation of intraarticular adhesion is a common complication after total knee arthroplasty or anterior cruciate ligament reconstruction. Previously, little research was reported regarding whether the local application of rapamycin (RAPA) could reduce intraarticular adhesion following knee surgery. In our present study, we determined...
Article
Hydroxycamptothecin (HCPT) has been proven to prevent intraarticular scar adhesion, but the mechanism is still unclear. ER stress is known to participate in many diseases, and the IRE-1 signal pathway has been reported in fibrotic diseases. The aim of this study was to illustrate the mechanism of HCPT-induced apoptosis in fibroblasts and the preven...
Article
Mitomycin C (MMC) is known to reduce epidural fibrosis, but the underlying mechanisms have not yet been elucidated. Aberrant miR-200b expressions have been reported in multiple types of fibrotic tissues from many diseases. The aim of this study was to clarify the mechanism by which MMC induces fibroblasts apoptosis and reduces epidural fibrosis. Th...
Article
Topical application of 10-hydroxycamptothecin (HCPT) can reduce intra-articular scar adhesion after knee surgery, but the effect of HCPT on wound healing has not yet been elucidated. The study investigates the effect of the topical application of HCPT on wound healing after knee surgery in rabbits. Forty New Zealand white rabbits were divided into...
Article
Full-text available
This study was aimed to compare the outcomes of two operational methods used for the fixation of calcaneal fracture, the open reduction using a plate and the minimally invasive cannulated screw fixations. Thus, we attempted to find out as to which of these fixation regimens was therapeutically superior by assessing improvement in the restoration of...
Article
This study aimed to explore the underlying molecular mechanisms of osteoarthritis (OA) by bioinformatics analysis. Synovial tissue samples from five OA and five normal donors (ND) were used to identify the differentially expressed genes (DEGs) by paired t-test. Pathway enrichment analysis of DEGs was performed, followed by construction of a protein...
Article
Full-text available
The aim of this study was to investigate the efficacy of topical application of colchicine in reducing intraarticular adhesion in rabbits. Thirty-six rabbits were randomly and equally divided into three groups. An approximately 10 × 10 mm(2) area of cortical bone was removed from both sides of the left femoral condyle, and the cancellous bone under...
Article
The objective of this study was to investigate the expression of adiponectin receptors (AdipoR1, R2, and T-cadherin) in both normal subjects and patients with knee osteoarthritis (OA). We used immunofluorescence to assess expression of adiponectin receptors in the chondrocytes of normal subjects (n = 3) and OA patients (n = 3). We also studied mRNA...
Article
Full-text available
10-Hydroxycamptothecin could reduce intraarticular adhesion by inhibiting fibroblasts proliferation after knee surgery. However, the ideal concentration of hydroxycamptothecin have not been defined. This study was tried to verify the optimal concentration of 10-hydroxycamptothecin in preventing knee intraarticular adhesion. Sixty rabbits were rando...
Article
There was no previous study about topical application of tacrolimus (FK506) could inhibit fibroblast proliferation and prevent epidural scar adhesion after laminectomy. We intended to illustrate the effect of FK506 on inhibiting fibroblast proliferation and preventing epidural scar adhesion after laminectomy in rat model. In our study, seventy-two...
Article
We sought to compare the preventive effects of mitomycin-C(MMC) and chitosan on intraarticular adhesion after knee surgery in rabbits. For this purpose, 48 New-Zealand rabbits were randomly and equally divided into MMC, chitosan, and control groups. Approximately 10 × 10 mm(2) of the cortical bone was removed from both sides of left femoral condyle...
Article
Mitomycin C (MMC) is known to inhibit fibroblast proliferation through suppressing DNA dependent RNA synthesis. Based on this knowledge, we illustrated the effect of MMC on inhibiting fibroblast collagen synthesis and reducing intraarticular fibrous adhesion in a rabbit model. Forty-eight New Zealand white rabbits were randomly divided into four gr...

Citations

... While previous studies have reported that the rate of femoral neck shortening ranged from 29 to 50.6% in the CS group [3,30], more recent studies have reported femoral neck shortening rates ranging from 0-19% for FNS fixation [14,31]. Finite element analysis and biomechanical study confirmed that FNS provides increased stability compared with CS for femoral neck fracture treatment [5,6,32]. The FNS has a 20-mm sliding compression space which can provide the direction of sliding pressure after reduction; thus, mitigating femoral neck shortening [33]. ...
... Femoral neck fractures often occur in the elderly, and the reason is that the physical function of the elderly is declining, and most of them are often complicated with chronic diseases such as hypertension and diabetes, with limited absorption of nutrients and obvious osteoporosis (11). At present, surgical treatment is widely used for hip fracture clinically, which can reduce the incidence and mortality of complications and has a good improvement effect (12). ...
... Cryptotanshinone inhibited the expression of cyclin D1, and the phosphorylation of Rb in human rhabdomyosarcoma Rh30 cells induced cell cycle arrest at the G0/G1 phase [28]. In addition, previous studies [21,22,29] also showed that tanshinones could inhibit cell migration [30] through a wide range of signaling pathways, i.e., the HIF-1α-EMT pathway [31], STAT3-CCL2-EMT [32], PI3K/AKT signaling [33], and the PI3K and AMPK-mTOR signaling pathway [34]. However, little is still known about the roles and mechanisms of tanshinones, including dihydrotanshinone I in the regulation of cell adhesion and cell migration [33,[35][36][37][38][39]. ...
... This seems reasonable, as the most recently published meta-analysis revealed no differences in overall reoperation rates between the two screw types. There were fewer cases of avascular necrosis when FNF was treated with CCS compared to SHS, particularly in displaced FNF, but CCS was removed more frequently than SHS [9]. The meta-analysis results were heavily weighted toward the largest of the randomized controlled trials (FAITH trial), which included 1,108 patients [10]. ...
... Personalized humerus reconstruction assisted by 3D printing based on radiographic projections using CT data is conducive to an intuitive understanding of fracture types and displacement directions [30]. Then, surgeons select optimal approaches and simulate procedures to reduce operation time with less bleeding and the frequency of use of fluoroscopies [31,32]. Our study demonstrated that 3D printingassisted surgery outperformed conventional operations in these aspects. ...
... RAD001 engages in the regulation of the PI3K/ AKT/mTOR pathway [68][69][70]. Next, we investigated the relationship between RAD001 and RTactivated PI3K/AKT/mTOR pathway by adding RAD001 after RT. The results revealed that treatment of RAD001 diminished the expression levels of PI3K, p-AKT, p-mTOR, p-S6K1, and p62, and elevated the LC3-II/LC3-I ratio and amount of acidic autophagic vesicles in CC cells. ...
... The TargetScan database predicted the presence of miR-150-5p binding sites in the 3′ untranslated region (3'UTR) of laminin α4 (LAMA4) mRNA. LAMA4 is a subunit of laminin macromolecule family and abundantly expressed in various fibrotic tissues [9]. Elevated LAMA4 can activate the p38 mitogenactivated protein kinase (MAPK) signaling pathway during senescence, leading to the development of age-related cataract [10]. ...
... The decellularized natural tissue have been thought of as repair material for implantation. In the field of cartilage defect repair, natural cartilage is difficult to decellularize due to its dense structure [8,9]. Therefore, the skin or peritoneum from pigs or cattle have been decellularized to remove cells and immunogenic proteins to make extracellular matrix (ECM)-based implants [10,11]. ...
... Therefore, the prevention of EF is of great significance to both surgeons and patients who require surgery [3,4]. Thus far, the pathogenesis of EF and scar adhesion has not been fully studied, however, most scholars believe that the activation, migration and excessive proliferation of fibroblasts in the surgical area are the main causes of the disease [5,6]. Therefore, we believe that adopting effective measures to prevent excessive proliferation of fibroblasts can serve as a reliable means in preventing EF. ...
... Specific cell markers can also help distinguish chondroprogenitors from the available pool of chondrocytes, notably following culture expansion when phenotypic drift following release from cartilage makes a comparison between the 2 cell groups difficult. 29,30 Additionally, a recent study comparing human chondroprogenitors and chondrocytes demonstrated that progenitors with increased time in culture exhibited significantly lower CD34, higher CD166, and CD146 levels. 19 Contrarily, a few studies have also suggested that eliminating CD146 positive subsets may improve chondrogenic ability. ...