Leyland Fraser's research while affiliated with University of Warmia and Mazury in Olsztyn and other places

Publications (320)

Article
Sperm functions may be influenced by seminal plasma (SP) proteins with affinity to zinc ions. The aim of the study was to isolate and characterise zinc-binding proteins (ZnBPs) from stallion SP using proteomic tools. Zinc-binding proteins were isolated from the SP of eight stallions by zinc-affinity chromatography. They were analysed in the SDS-PAG...
Conference Paper
The electrophoretic patterns and expressions of boar sperm proteins in good and poor freezability ejaculates (GFE and PFE, respectively) were investigated using SDS-PAGE and western blotting (WB). The Neuman-Keuls post hoc test was used to identify significant differences in the sperm parameters between the GFE and PFE groups. Boars of the GFE (n=5...
Article
Full-text available
The aim of the study was to determine the relation between the semen quality, frequency of sperm defects, sperm dimensions and shape, and the ejaculate volume of Large White and Landrace boars. A total of 648 ejaculates collected from 31 Large White and 30 Landrace boars were divided into three groups according to the criterion of the ejaculate vol...
Article
Full-text available
Parameters of sexual activity were determined in 49 young boars used for artificial insemination, four times at three-month intervals. The parameters included the time from entering the arena until mounting the phantom; the time from mounting the phantom until achieving erection; the time from achieving full erection until the start of ejaculation;...
Conference Paper
Full-text available
Many important functions of spermatozoa are influenced by semi- nal plasma (SP) proteins, which showed affinity for zinc ions. The aims of the study were to isolate zinc-non-binding (nZnBPs) and zinc- binding proteins (ZnBPs) of stallion SP and analyze them using gene ontology and label-free quantification (LFQ). The nZnBPs and ZnBPs were isolat...
Article
Full-text available
This study aimed to characterize the protein composition of fractionated seminal plasma (SP) by liquid chromatography mass spectrometry (LC–MS/MS) analysis and investigate its effects on survival of frozen-thaw (FT) boar spermatozoa following storage. Seminal plasma (SP) was fractionated by gel filtration chromatography to give two fractions, SP1 w...
Article
Full-text available
The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D),...
Article
Single nucleotide polymorphisms (SNPs) in the 5'-flanking regulatory regions of genes could affect their expression levels. This is a follow-up study aimed to identify polymorphic variants in the 5'-flanking regulatory regions of genes expressed in boar spermatozoa, and to predict the interactions of such variants with transcription factors (TFs) o...
Article
Long non-coding RNAs (lncRNAs) are suggested to play an important role in the sperm biological processes. We performed de novo transcriptome assembly to characterize lncRNAs in spermatozoa, and to investigate the role of the potential target genes of the differentially expressed lncRNAs (DElncRNAs) in sperm freezability. We detected approximately 4...
Article
Full-text available
During the early stages of pregnancy, the uterine endometrium undergoes dramatic morphologic and functional changes accompanied with dynamic variation in gene expression. Pregnancy-stage specific differentially expressed gene (DEG)-transcript-probes were investigated and identified by comparing endometrium transcriptome at 9th day (9D), 12th day (1...
Article
Genetic markers have been used to assess the freezability of semen. With the advancement in molecular genetic techniques, it is possible to assess the relationships between sperm functions and gene polymorphisms. In this study, variant calling analysis of RNA-Seq datasets was used to identify single nucleotide polymorphisms (SNPs) in boar spermatoz...
Conference Paper
Besides variations in the sperm transcriptome, alterations in the proteome of spermatozoa affect their freezability. The main objective of this study was compare the electrophoretic profiles of membrane proteins in spermatozoa from boars with good and poor semen freezability (GSF and PSF, respectively). Post-thaw (PT) semen of 40 Polish large white...
Article
Semen freezability is associated with genetic markers, and there is a diverse set of sperm transcripts that have been attributed to various cellular functions. RNA-Seq was performed to compare the transcript profiles of spermatozoa from boars with different semen freezability. We examined ejaculates from the Polish large white (PLW) boars that were...
Article
The binding of seminal plasma (SP) proteins by spermatozoa plays an important role in the regulation of sperm epididymal maturation, motility gaining in female reproductive tracts and sperm-egg interaction. The aim of the study was to analyze the SP and sperm extracts proteome of cat (Felis catus) semen. The seminal plasma and spermatozoa were obta...
Conference Paper
This study investigated the effect of post-thaw (PT) supplementation of fractionated seminal plasma (SP) from boars with good and poor semen freezability (GSF and PSF, respectively) on the sperm characteristics. Two fractions, SP1 (>40 kDa) and SP2 (<40 kDa), were obtained by affinity chromatography of SP from four and three boars with GSF and PSF,...
Article
Electrophoretic methods were used to identify protein complexes formed between ostrich egg yolk lipoprotein fractions (LPFo) with seminal plasma (SP) of fractionated ejaculates, and to investigate the effect of these complexes on boar semen quality after cryopreservation. Chromatographic SP fractions (F1, F2 and F3), with or without LPFo solution,...
Article
In this study we investigated the effect of post-thaw (PT) supplementation of fractionated SP (SP1, >40 kDa; SP2, <40 kDa), on the survival of spermatozoa from boars with good and poor semen freezability, GSF and PSF, respectively. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis showed distinct differences in the protein profi...
Article
Full-text available
The aim of this study was to identify the proteoforms of albumin and kallikrein in stallion seminal plasma (SP), and to determine their correlations with sperm motility parameters. The experimental material consisted of ejaculates from 8 stallions, which were collected during the breeding and non-breeding seasons (BS and NBS, respectively). SP prot...
Article
This study aimed to investigate the effect of fractionated seminal plasma (SP) on boar sperm characteristics following cryopreservation. Gel filtration chromatography yielded two fractions: SP1 with >40 kDa and SP2 with <40 kDa. The fractionated SP (SP1 and SP2), whole seminal plasma (wSP) and BTS were used for the treatment of semen before freezin...
Article
In this study the quality of total RNA, isolated from fresh spermatozoa, was compared between boars with good and poor semen freezability (GSF and PSF, respectively). Semen from 3 boars with GSF exhibited significantly higher total motility, mitochondrial function, plasma membrane integrity and reduced lipid peroxidation compared with 3 boars with...
Conference Paper
In this study different software packages were used to analyze the differentially expressed (DE) gene transcripts in spermatozoa of the Polish large white (PLW) boar differed in semen freezability. Total RNA, isolated from spermatozoa of four boars with good and poor freezability each, was subjected to RNA-Seq. An average of 8 million reads per sam...
Conference Paper
In this study transcriptome sequencing was performed in fresh spermatozoa of three Polish large white (PLW) boars each with good and poor semen freezability (GSF and PSF, respectively). Total RNA was extracted from spermatozoa and RNA-Seq library preparation was used for paired-end sequencing on the NextSeq 500 system (Illumina). Common gene transc...
Conference Paper
Full-text available
This study investigated the effects of the supplementation of fractionated seminal plasma (SP) to post-thaw semen on boar sperm cryo-survival. Post-thaw semen of seven boars was split into three parts and each portion was incubated with chromatographically fractionated SP (proteins >40 kDa, SP 1; proteins <40 kDa, SP 2), with (1:1) or without BTS e...
Conference Paper
This study investigated the profiles of differentially expressed (DE) gene transcripts of spermatozoa from the Polish large white (PLW) boars differed in semen freezability using RNA-Seq. Total RNA was isolated from fresh spermatozoa of the PLW boars, classified as good and poor semen freezability (n=3, respectively), and was used for RNA-Seq. Tran...
Conference Paper
Sperm cells are thought to be transcriptionally inactive, however, they contain a complex collection of remnant RNAs, which are implicated in the sperm-egg fertilization processes and early embryonic development. Isolation of total RNA from spermatozoa is always a challenging task, mainly due to the extremely low RNA content and relatively high DNA...
Conference Paper
This main objective of this study was identify differences in the gene expression profiles of spermatozoa from the Polish large white (PLW) boars with different semen freezability. The semen of three PLW boars was classified as good and poor freezability (GSF and PSF, respectively. Total RNA was isolated from fresh spermatozoa of the PLW boars. Pri...
Conference Paper
This study aimed to investigate the proteomic characteristics of fractionated SP and to assess its relationships with the freezability of boar semen. Boar sperm-rich fractions were split into three portions and each portion was incubated with chromatographically fractionated SP comprising proteins with more than 40 kDa (F1) and less than 40 kDa (F2...
Conference Paper
In this study transcriptome sequencing (RNA-Seq) was used to compare the expression gene profiles between spermatozoa RNA isolated from Polish large white boars with good and poor semen freezability (GSF and PSF, respectively). Total RNA was isolated from spermatozoa of three GSF (n = 3) and three PSF (n = 3) boars, using a modified extraction proc...
Article
Lipoproteins, isolated from ostrich egg yolk (LPFo), provide excellent protection for boar spermatozoa against cryo-induced damage. The present study was performed to investigate the effects of LPFo on the freezability and fertilizing capacity of frozen-thawed (FT) boar semen after post-cervical artificial inseminations (post-CAIs). Semen, collecte...
Article
Assessment of sperm-DNA integrity is a crucial issue in male fertility. In the present study, parameters derived from the image analysis of comets after single-cell gel electrophoresis were used to analyse the types of DNA damage of frozen–thawed boar spermatozoa. Semen, frozen in a cryoprotectant-free extender or in cryoprotectant-based extenders,...
Conference Paper
This study investigated the effects of fractionated seminal plasma (SP) from boars with good and poor semen freezability (GSF and PSF, respectively) on motility characteristics and membrane integrity of frozen-thawed (FT) spermatozoa. Sperm-rich fractions, collected from 4 boars with GSF and 3 boars with PSF, were split into three parts, and each p...
Conference Paper
In this study several parameters of the sperm quality characteristics were used to identify boars with good and poor semen freezability (GSF and PSF, respectively). Semen, collected from ten Polish Large White boars, was frozen using a cryopreservation protocol. Sperm total and progressive motility (TMOT and PMOT, respectively) were analyzed with t...
Poster
Analysis of the sperm quality characteristics in boars differing in semen freezability has confirmed intrinsic differences in the susceptibility of spermatozoa from individuals to cryo-induced damage.
Article
This study investigated individual boar variability in the quality of pre-freeze (PF) and post-thaw (PT) semen cooled in different long-term (LT) extenders and for different holding times (HT). Sperm rich fractions were diluted with Androhep® Plus (AHP), Androstar® Plus (ASP), Safecell® Plus (SCP) and TRIXcell® Plus (TCP) extenders, stored for 2 h...
Conference Paper
The quality of post-thaw semen is affected by different factors such as male variability, sperm treament and extender composition. This study investigated the effects of different seminal plasma (SP) fractions on the viability of boar spermatozoa following cryopreservation. The sperm-rich frac-tions (SRFs), collected from three PLW boars, were divi...
Conference Paper
Male variability in semen freezability is one of the main factors affecting sperm cryo-survival in different animal species. In this study different sperm quality parameters were used to characterize boars with good and poor freezability semen (GFS and PFS, respectively). Semen, collected from 18 Polish Large White boars, was frozen using a cryopre...
Conference Paper
Celem badań była analiza wpływu różnych wariantów rozcieńczalnika na właściwości biologiczne kriokonserwowanych plemników knura, z uwzględnieniem sposobu kolekcjonowania nasienia. Ejakulaty dzielono podczas pobierania na dwie części: gęstą frakcję nasienia oraz pozostałość ejakulatu. Próby nasienia poddawano procesowi zamrażania-rozmrażania. Po roz...
Conference Paper
Full-text available
Proteomic analysis was used to characterize the non-phosphorylcholine-binding and phosphorylcholine-binding proteins (nPchBPs and PchBPs, respectively) of canine semial plasma (SP). Ejaculates, collected from five healthy crossbred dogs (n=25), were centrifuged to separate the spermatozoa from SP. The nPchBPs and PchBPs were isolated from canine SP...
Chapter
Advances in sperm assessment techniques have offered new perspectives to improve the technology of semen cryopreservation. This review addresses some recent achievements in the proteomics of seminal plasma and spermatozoa and exemplifies its importance as markers for sperm fertility following cryopreservation. Recent advances in transcriptome studi...
Conference Paper
This study investigated the effects of fractionated seminal plasma (SP) on quality characteristics of boar spermatozoa following freezing-thawing. Boar sperm-rich fractions (SRFs) were split into three parts and each portion was incubated with chromatographically fractionated SP comprising proteins less than 40 kDa (SP 1) and more than 40 kDa (SP 2...
Conference Paper
In this study the TRIzol/PureLink RNA protocol was used to extract total RNA from fresh spermatozoa of boars differed in semen freezability. The Neuman–Keuls post hoc test showed that boars with poor and good semen freezability were characterized by a significant reduction in CASA-analyzed sperm motility, mitochondrial function analyzed by the JC-1...
Conference Paper
Revolutionized high-throughput next-generation genome sequencing (HT-NGS) based RNA-seq technologies and advancements in bioinformatics tools are challenging task to analyse the big transcriptomic data of domestic animal genome including Sus scrofa. In this study the NextSeq 500 (Illumina) NGS platforms were used to give a brief outline of the tran...
Article
Full-text available
Abstract Seminal plasma (SP) proteins participate in the process of fertilization by binding to the sperm membrane, particularly to the phosphorylcholine-containing lipids. This study aimed to identify and characterize non-phosphorylcholine-binding and phosphorylcholine-binding proteins (nPchBPs and PchBPs, respectively) of canine SP. The nPchBPs a...
Article
There has been increased improvement in the cryopreservation protocol of semen, which contributes to the preservation of genetic resources Cryo-induced damage to spermatozoa is attributed to membrane deteriorations caused mainly by the formation of intracellular ice and increased oxidative stress, resulting in a loss of the sperm fertilizing abilit...
Article
The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures. Boar ejaculates were extended in Androhep, BTS, and MR-A without (control) or with the addition of LPFo and stored for th...
Article
This study investigated the relationships between lipid peroxidation (LPO) and sperm DNA damage following freezing-thawing of boar semen in different extenders. The comet assay was used to measure the extent of sperm DNA damage in a cryoprotectant-free extender or in cryoprotectant-based extenders following single, and repeated freezing and thawing...
Article
Full-text available
The aim of this study was to evaluate the effect of lipoprotein fraction isolated from ostrich egg yolk (LPFo) on the metabolic activity of boar spermatozoa following liquid semen storage in different extenders and temperatures. Boar ejaculates were extended in Androhep, Beltsville thawing solution (BTS), and Martín-Rillo and Alias (MR-A) without (...
Data
RNA-seq SNP-db of bovine liver tissue of young bull-14 of Hereford cattle aged 6 months. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-2 of Polish Red cattle aged 6 months. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-7 of Polish HF cattle aged 6 months. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-17 of Hereford cattle aged 12 months. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-4 of Polish Red cattle aged 9 months. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-12 of Polish HF cattle aged 12 months. (XLSX)
Data
Identification of De novo SNPs data set in Hereford breed. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-5 of Polish Red cattle aged 12 months. (XLSX)
Data
SNPs filtering data set with a minimum depth of 10 SNP reads that support the polymorphic nucleotide base and SNP ratio = 100% of Hereford breed. (XLSX)
Data
Lists of 110 SNP loci within the CGs bovine growth trait with representing full names, UMD3.1 genome locations, chromosomal locations and web links at bovine QTL-DB. (XLSX)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-8 of Polish HF cattle aged 6 months. (XLSX)
Data
Breed comparison in SNPs filtering data set of Polish Red with Polish HF breed. (XLSX)
Data
Summary of identified putative SNPs hits of liver RNA-seq data on 110 potential candidate genes from bovine QTL-db in Polish Red cattle. (XLS)
Data
RNA-seq SNP-db of bovine liver tissue of young bull-9 of Polish HF cattle aged 9 months. (XLSX)
Data
Breed comparison in SNPs filtering data set of Polish HF with Polish Red and Hereford breeds. (XLSX)
Data
RNA-seq experimental results of nine selected breed-specific SNPs loci utilized in SNP validation experiment. (XLSX)
Data
SNPs filtering data set with no errors of Polish Red breed. (XLSX)
Data
Identification of putative SNPs hits of liver RNA-seq data on 110 potential candidate genes from bovine QTL-db in Polish Red cattle. (XLS)
Data
Distribution of KASPTM genotypes of nine selected breed-specific SNPs originating from the bovine liver RNA-seq experiment. (XLSX)
Data
SNPs filtering data set with no errors of Hereford breed. (XLSX)
Data
Identification of putative SNPs hits of liver RNA-seq data on 110 potential candidate genes from bovine QTL-db in Polish HF cattle. (XLS)
Data
Genetic differentiation comparison of SNP genotypes among cattle breeds using the Fisher's Exact G test. (DOC)
Data
SNPs filtering data set with a minimum depth of 10 SNP reads that support the polymorphic nucleotide base of Polish Red breed. (XLSX)
Data
Identification of putative SNPs hits of liver RNA-seq data on 110 potential candidate genes from bovine QTL-db in Hereford cattle. (XLS)
Data
SNPs filtering data set with a minimum depth of 10 SNP reads that support the polymorphic nucleotide base of Polish HF breed. (XLSX)