Leonard R. Axelrod's scientific contributions
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It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
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Publications (3)
Human fetal testes were separately incubated with progesterone-4-C14 and 5-pregnenolone-7α-H3 as substrates. The isolated and identified metabolites showed that an array of enzymes, including 3β-ol-dehydrogenase, 17β-ol-dehydrogenase, side-chain splitting, 20α- and β-ol-dehydrogenases, 21-hydroxylase, 17α-hydroxylase and 16α-hydroxylase, were activ...
Citations
... GC-MS (and GLC) made it possible to identify and quantify not only T but also its endogenous precursors in gonadal extracts. Indeed, in the pooled testes of foetuses of 10-22 PCW (12-24 GW) in age, nine neutral steroids (unconjugated monosulphates or disulphates) were found, including 3β,16α-dihydroxy-5-androsten-17-one, which is the products of the 16α-hydroxylating activity and previously shown to be present in in vitro studies [122,124]. ...
... The buffer frequently used is Krebs-Ringer-phosphate-glucose pH 7.4. The reaction is stopped with chloroform:methanol (2:1), or acetone, and then the radioactive metabolites are extracted (with ethyl acetate or ethanol) and re-dissolved in a mixture of chloroform:methanol (2:1) [23,24]. The radioactive metabolites produced by the precursor during this incubation are then quantified. ...
