Laurent Tiers's research while affiliated with Unité Inserm U1077 and other places

Publications (52)

Article
Background: Little is known about the influence of Riluzole on serum neurofilament light chain (sNfL) levels, a biomarker of prognosis in amyotrophic lateral sclerosis (ALS), and variations with time of sNfL concentrations are controversial. Methods: Sera from ALS patients (n = 141) and controls (n = 33) were collected at inclusion (sNfL1) and s...
Article
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The present study focuses on the use of a metaproteomic approach to analyze Black Extrinsic Tooth Stains, a specific type of pigmented extrinsic substance. Metaproteomics is a powerful emerging technology that successfully enabled human protein and bacterial identification of this specific dental biofilm using high-resolution tandem mass spectromet...
Article
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Background The diagnosis of skin wound vitality is currently based on standard histology, but histological findings lack sensitivity in case of a short survival time. New reliable biomarkers of vitality are therefore strongly needed. We assessed the ability of 10 candidate cytokines (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF...
Article
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Background Tau proteins are recognized biomarkers of neurodegeneration and neuronal damage in the cerebrospinal fluid (CSF). It has also been suggested that these CSF proteins could increase post-mortem due to neuronal death. The aim of this study was to investigate the changes in CSF total and phosphorylated tau (p-tau) levels in the early post-mo...
Article
In patients with metastatic breast cancer (MBC), brain metastases (BM) are associated with high morbidity and mortality. However, there is no validated serum biomarker that accurately predicts BM occurrence in these patients, and the role of serum biomarkers for prognosis remains unclear. Here, we evaluated the association of Neurofilament light ch...
Article
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Proteomic technologies have been recently adapted to the new field of clinical proteomics. The origin of errors and biases has been well-identified in the pre-analytical steps, leading to the measurement of clinical analytes. One possible source of inadequacy in clinical proteomics is linked to sample pooling. This practice is usually related to lo...
Presentation
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Clinical proteomics approaches rely mostly on the absolute quantification of specific targeted proteins and/or on global proteome quantification following a bottom-up approach [1, 2]. These quantitative data picture a global proteome in a steady-state. In this context, disease-relevant proteins or biomarkers are identified via detected differences...
Poster
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Background: Monoclonal antibody (mAb) is the first class of biotechnological products followed by recombinant proteins and vaccines. Bevacizumab (AVASTIN) product by Roche, is able to neutralize Vascular Endothelial Growth factor (VEGF), a regulator of angiogenesis and surexpressed in a lot of human tumors. By combining a step of Protein-A capture...
Poster
Full-text available
Monoclonal antibody (mAb) is the first class of biotechnological products followed by recombinant proteins and vaccines. Bevacizumab (AVASTIN®) product by Roche, is able to neutralize Vascular Endothelial Growth factor (VEGF), a regulator of angiogenesis and surexpressed in a lot of human tumors. The analytical strategy was designed to quantify the...
Article
Full-text available
Human biological fluids are complex matrices containing many elements (ions, lipids, sugars, proteins, etc.). Proteins are essential for biomarker and disease discovery though the most abundant proteins often provide very limited clinically relevant data, as can be the case for albumin or immunoglobulins. In this work, we focused on depleting album...
Article
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Background: Cerebrospinal fluid (CSF) biomarkers (Aβ peptides and tau proteins) improved the diagnosis of Alzheimer’s disease (AD) in research and clinical settings. We previously described the PLM-scale (Paris-Lille-Montpellier study), which combines Aβ42, tau, and phosphorylated ptau(181) biomarkers in an easy to use and clinically relevant way....
Article
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Aim: Monoclonal antibody-based treatment of cancer has been established as one of the most successful therapeutic strategies. Materials & methods: In this work, we developed a workflow based on an automated protein-A capture and LC-MS/MS analysis to quantify bevacizumab on patient serum during treatment. This analytical approach was fully valida...
Article
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Laccase enzymes produced by Botrytis cinerea are involved in the oxidation of phenolic substances during the development of grey mould, which causes significant economic losses. The aim of this work was to study the structural and activity characteristics of the laccase enzymes secreted by three B. cinerea strains that are involved in the developme...
Poster
Full-text available
Apolipoprotein E (Apo E) is a 36 Kda glycoprotein involved in lipid transport. It exists in 3 major isoforms: ε2, ε3 and ε4. Apo E status is known to be a major risk factor for late-onset Alzheimer’s and cardiovascular diseases. Genotyping is commonly used to obtain Apo E status but can show technical issues with ambiguous determinations. Phenotypi...
Article
Full-text available
Although dried blood spot (DBS) sampling methods have been used since the 1960s, they have recently attracted renewed interest because of the development of new clinical applications. In addition to their other advantages, DBS methods can now be used to quantify many blood proteins using the latest highly sensitive and robust, liquid chromatography...
Article
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Multiple myeloma (MM) is a plasma cell cancer with poor survival, characterized by the expansion of multiple myeloma cells (MMCs) in the bone marrow. Using a microarray-based genome-wide screen for genes responding to DNA methyltransferases (DNMT) inhibition in MM cells, we identified RECQ1 among the most downregulated genes. RecQ helicases are DNA...
Presentation
Full-text available
Monoclonal antibody (mAb) is the first class of biotechnological products followed by recombinant proteins and vaccines. Bevacizumab (AVASTIN) product by Roche, is able to neutralize Vascular Endothelial Growth factor (VEGF), a regulator of angiogenesis and surexpressed in a lot of human tumors. By combining a step of Protein-A capture and a LC-MRM...
Article
Full-text available
In assisted reproduction, about 30% of embryo implantation failures are related to inadequate endometrial receptivity. To identify molecules involved in endometrial receptivity acquisition, we investigated, using a SELDI-TOF approach, the protein expression profile of early-secretory and mid-secretory endometrium samples. Among the proteins up-regu...
Article
Background: Apolipoprotein E (Apo E) is a 36Kda glycoprotein involved in lipid transport. It exists in 3 major isoforms: E2, E3 and E4. ApoE status is known to be a major risk factor for late-onset Alzheimer's and cardiovascular diseases. Genotyping is commonly used to obtain ApoE status but can show technical issues with ambiguous determinations....
Article
Background: Detection of biomarkers in the CSF has proven to be useful to follow and understand the metabolism of molecular actors of Alzheimer's disease (AD), as well as, for diagnosis purposes. Immunodetection is mostly used to detect and quantify these biomarkers but this approach has sometime a poor specify and a limited detection range of the...
Patent
The invention relates to a method for predicting the response to an interferon-based treatment in a patient infected with hepatitis C virus. This method consists in determining the presence of apolipoprotein CIII and/or of a multimeric form of human serum albumin in a sample of biological fluid from the patient.
Article
Full-text available
Background: The use of dried blood spots on filter paper is well documented as an affordable and practical alternative to classical venous sampling for various clinical needs. This technique has indeed many advantages in terms of collection, biological safety, storage, and shipment. Amyloid β (Aβ) peptides are useful cerebrospinal fluid (CSF) biom...
Article
Introduction Randomized controlled trials have shown laparoscopic removal of endometriosis to be advantageous in improving fertility outcomes for women with minimal-mild disease. There are limited data available on fertility outcomes following surgery for stage 3 and 4 endometriosis. Materials and Methods In this retrospective study at a tertiary...
Article
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Clinical Proteomics biomarker discovery programs lead to the selection of putative new biomarkers of human pathologies. Following an initial discovery phase, validation of these candidates in larger populations is a major task that recently started relying upon the use of mass spectrometry approaches, especially in cases where classical immune-dete...
Conference Paper
Objectives: The goal of this study is to compare protein profiles of saliva and gingival crevicular fluid against sera in order to get more insights concerning the clinical potentiality of the two oral fluids. Methods: Saliva and GCF were obtained from healthy nonsmoking subjects in the morning at least 2 h after eating and after rinsing mouth wi...
Article
Full-text available
To improve the etiological diagnosis of neurodegenerative dementias like Alzheimer's disease (AD) or frontotemporal dementia (FTD), we evaluated the value of individual and combined measurements of the following relevant cerebrospinal fluid (CSF) biomarkers: Tau, 181p-Tau, Aβ38, Aβ40, Aβ42, sAβPPα, and sAβPPβ. This study conducted in two centers in...
Article
Cerebrospinal fluid (CSF) biomarkers are now widely used for diagnosis of Alzheimer disease (AD) in atypical clinical forms, for differential and early diagnosis, or for stratification of patients in clinical trials. Among these biomarkers, different forms of amyloid peptides (Aβ) produced by the cleavage of a transmembrane precursor protein called...
Article
Severe asthma is characterized by neutrophilic inflammation and high levels of interleukin (IL)-8. Airway epithelial cells play a pivotal role in the pathogenesis and chronicity of asthma. The objective of this work was to determine whether CXC receptors were involved in human small airway epithelial cell (SAEC) activity by incubating them with IL-...
Conference Paper
Introduction Le diagnostic positif des affections neurodégénératives et en particulier de la maladie d’Alzheimer (MA) devient une exigence pour la prise en charge des patients et pour l’évaluation des nouvelles molécules “anti-démence”. Actuellement, le diagnostic de MA combine de données anamnestiques, cliniques, neuropsychologiques, d’imagerie mo...
Article
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Depletion of major blood proteins is one of the most promising approaches to access low abundant biomarkers using proteomics. Immunocapture columns often used for this purpose exist in different formats depending on the number of major proteins removed. In this article, we compared the relative interest of depleting either one (albumin), six (album...
Article
Detection of autoantibodies, which are involved in tissue injury and/or the reporters from the immune system of various pathologic events, has an important potential for diagnosis, prognosis, disease staging and treatment selection. This explains the interest for new proteomics technologies, such as the high-density protein microarray used here, th...
Article
Full-text available
In this manuscript, we compared serum profiles obtained with two related technologies, SELDI-TOF and Clinprot, using a single bioinformatic algorithm. These two approaches rely on mass spectrometry to detect proteins and peptides initially selected by binding to various chromatographic matrices. They are proposed by two different companies, and the...
Article
Full-text available
Tissue and functional regeneration takes place in the body at various stages throughout life. However, bone, cartilage, tendons, blood vessels and cardiac muscle have a limited capacity for self repair and, after injury or disease, the regenerative ability of these adult tissues is often insufficient and leads to nonfunctional scar tissue. In this...
Article
Full-text available
Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) has been proposed as new approach for blood biomarker discovery. However, results obtained so far have been often disappointing as this technique still has difficulties to detect low-abundant plasma and serum proteins. We used a serum depletion scheme using chicken antibodies...
Article
Full-text available
In this manuscript, we compared serum profiles obtained with two related technologies, SELDI-TOF and Clinprot, using a single bioinformatic algorithm. These two approaches rely on mass spectrometry to detect proteins and peptides initially selected by binding to various chromatographic matrices. They are proposed by two different companies, and the...

Citations

... Thereby, when tyrosine kinase inhibitor targeting endothelial VEGF was used, it inhibited angiogenesis with a concurrent decrease in BrM. A recent study assessed a panel of proteins in serum and found glial fibrillary acidic protein associated with the potential of BC to metastasize to brain; however, this still needs to be assessed in different or large cohorts of patients [53]. In a patient study, C-reactive protein levels in the blood distinguished the brain metastatic patients from glioblastoma, that included 29 patients; however, primary tumor origin was unknown, but this could be a good diagnostic marker if it stood thorough validations [48]. ...
... We included in the present study only recent wounds, with a survival or pre-devascularization time of few seconds or minutes in a significant number of cases, which may explain the low sensitivity, whatever the method. In the same series of medicolegal wound, we previously found a sensitivity of 21% for the evaluation of IL8 staining, which reached 46% when using IL8 in a multiplex immunoassay, normalized on healthy skin levels (32,33). Hence, we can conclude that IHC is less sensitive that immunoassay, but the latter has the disadvantage of needing fresh frozen tissue and to be a method which is not as largely developed as IHC, requiring training sets and data normalization, without morphological control. ...
... Both groups demonstrated significantly higher serum NfL levels than healthy adult controls (7.2 pg/mL) [20]. Darlix et al. also demonstrated significantly higher median serum levels of NfL in patients with breast cancer metastatic to the brain compared to those without IMD (41.3 pg/mL vs. 23.6 pg/mL) [21]. ...
... Another study revealed that Srsf3 expression is increased in the hippocampus and temporal cortex of AD patients and suggested that its activity modulates tropomyosin receptor kinase B (TrkB) pre-mRNA splicing to promote neurodegeneration (Wong et al., 2012). Furthermore, Srsf3 was also shown to affect alternative splicing of the tau gene, one of the main biomarkers of AD (Yu et al., 2004;Peyron et al., 2021). The above observations are in line with our study in suggesting that inhibition of Srsf3 expression mediates, at least in part, the beneficial effect of selenium on AD pathology. ...
... Le taux de protéines retenues et d'environ 96%. Grâce à cela, 20 protéines n'ayant jamais été observées expérimentalement ont pu être validées par analyse de spectrométrie de masse[95].D'autres techniques existent aussi pour la déplétion d'albumine comme le Cibacron bleu[101,102] ou le kit AlbuVoid de Biotech Support[103]. Ces méthodes sont moins onéreuses et plus faciles à mettre en oeuvre. Cette dernière approche permet une déplétion de 91% de l'albumine. ...
... As an essential improvement to the BTI approach previously described by Zaccaria et al. [12], our method did not make any modifications to the standard surgical DBS procedures, and our approach allowed routine sample collections from the guide tubes from both hemispheres of the patients, without the need for sample pooling for subsequent analyses. Pooling of samples may increase the number of proteins identified, but at the same time, it masks sample-specific proteoforms and post-translational modifications and may lead to the loss of valuable information on individual patients [42]. For their analysis, Zaccaria et al. pooled samples from six patients and both hemispheres for in-gel fractionation and subsequent MS analysis [12]. ...
... As a result of this analysis, a novel stratification method was proposed, which overcomes the variability observed in the conventional method of stratification. It is worth to notice that, despite this study was performed in healthy donors, its findings could be relevant for the stratification of patients by sEGF levels, in diseases for which the usefulness of this molecule as a biomarker has been previously established: in diagnosis [15][16][17], prognosis [18,19], determination of patient's eligibility for treatment [20][21][22] and monitoring of the therapy in a clinical setting [17,[23][24][25][26]. The results of the present study might also help to understand the kinetics of the release of EGF by the platelets, thus contributing to a better reading of the levels of this molecule in sera of healthy and sick individuals. ...
... Specifically, a decrease in the pathogenic Aβ42 isoform or ratio of Aβ42/Aβ40 from the Vandermeeren, Mercken et al. 1993, Arai, Terajima et al. 1995, Tato, Frank et al. 1995, Nelson et al. 2012, Crary et al. 2014 CSF correlates with an increase in Aβ aggregates in the brain, which is largely due to a decrease in Aβ clearance during late onset AD disease (Mawuenyega et al. 2010;Palmqvist et al. 2014;Palmqvist, Mattsson et al. 2016). The ratio of Aβ42/ Aβ40 or Aβ42/total tau (t-tau) or Aβ42/phosphorylated tau (p-tau) are shown to be better correlated with positive Aβ PET imaging in AD patients than any one of these single markers alone (Shaw et al. 2009;Lehmann et al. 2018). However, CSF Aβ42 levels can vary based upon differences in pre-analytical samples and storage, individual differences in amyloid levels between patients, and decreased brain volume Shaw et al. 2009;Shaw et al. 2011). ...
... The LC-MS/MS methods previously published for measuring plasma mAb levels were appropriately validated following the EMA guidelines [47]. However, very few of them were cross-validated with another method [22,24,28,30,[51][52][53]. In a context of TDM, the cross-validation issue is critical for determining whether the obtained data are reliable, and whether they can be compared and used with respect to data from the literature. ...
... Since the protease preparation was reduced to pH 1.5 prior to ammonium sulfate precipitation, it is not certain that any of the proteases identified by SWATH-MS were able to maintain enzymatic activity and contribute to the overall protease activity recorded in the final product. However, the EnzChek® protease assay used for quantifying protease activity in the samples will detect metallo, serine, sulfhydryl, and acid proteases (BcAP8) (Probes 2004). Therefore, while it is likely that most of the protease activity detected in the B. cinerea protease mixture was BcAP8, as it is the most abundant protein in the B. cinerea secretome and in this sample Ten Have et al. 2010) and known to be active at low pH, it is possible that serine and metallo-proteases also added to the protease activity detected by EnzChek® assay. ...