Laurent Riquier’s research while affiliated with French National Institute for Agriculture, Food, and Environment (INRAE) and other places

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Publications (35)


Does Saccharomyces cerevisiae play a supporting role in mousy off-flavours production?: Original language of the article: English.
  • Article
  • Full-text available

March 2025

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6 Reads

IVES Technical Reviews vine and wine

Pierre Moulis

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The alteration known as "mousy taint" is a resurgent deviation in certain wines, characterised by reminiscent aromas of rice cakes and sausage skins. Saccharomyces cerevisiae, the microorganism responsible for alcoholic fermentation in wines, is crucial for winemaking and has not been directly linked to this defect. However, recent studies suggest that some strains of S. cerevisiae may indirectly affect the production of mousy N-heterocycles in a synthetic medium.

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FIGURE 1. Comparison of the production of ATHP (A, B, C) and ETHP (D, E, F) by the three strains of Brettanomyces bruxellensis (CRBO L0417, CRBO L1751, YJS 7816) and three Pediococcus parvulus (DSM 20332, CRBO 0202, CRBO 0601) co-inoculated or separately in 24 h at 25 °C in NHAM; comparisons were made by a Wilcoxon test, the associated p-values are indicated (*: p-values < 0.05).
FIGURE 2. Comparison of the production of APY (A, B, C) by the three strains of Brettanomyces bruxellensis (CRBO L0417, CRBO L1751, YJS 7816) and three Pediococcus parvulus (DSM 20332, CRBO 0202, CRBO 0601) coinoculated or separately in 24 h at 25 °C in NHAM; comparisons were made by a Wilcoxon test, the associated p-values are indicated (*: p-values < 0.05).
FIGURE 3. Representation of the concentration of ETHP as a function of the concentration of ATHP, with a Spearman р = 0.67 (p-value = 1.70 × 10 -10 ).
FIGURE 4. Relative areas of ATHP and ETHP produced by three strains of Brettanomyces bruxellensis (CRBO L0417, CRBO L1751, YJS 7816) co-inoculated (or not) with three strains of Saccharomyces cerevisiae (FX10, GN, SB); the standard deviation represents the deviation between the 2 chemical analyses.
FIGURE 5. Comparison of the relative areas of ATHP (A) and ETHP (B) produced by three strains of Brettanomyces bruxellensis (CRBO L0417, CRBO L1751, YJS 7816) in the presence (or not) of three Saccharomyces cerevisiae strains (FX10, GN, SB) in 24 h at 25 °C in NHAM, depending on the strain of S. cerevisiae; comparisons were made by an ANOVA and Tukey post-hoc tests.

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Impact of Pediococcus parvulus and Saccharomyces cerevisiae on Brettanomyces bruxellensis mousy compound production

September 2024

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62 Reads

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3 Citations

OENO One

The presence of mousy off-flavours in wine, attributed to the production of N-heterocycles by lactic acid bacteria or Brettanomyces bruxellensis, poses a significant challenge in the winemaking industry. This study is the first one to investigate the impact of co-cultures focusing on B. bruxellensis with two common wine microorganisms, Pediococcus parvulus and Saccharomyces cerevisiae on the formation of mousy compounds. The screening was conducted in a synthetic medium under controlled conditions. It reveals the nuanced effects of the microorganism combinations. Depending on the P. parvulus or S. cerevisiae strain co-inoculated with B. bruxellensis a synergistic effect is observed on the 2-acetyltetrahydropyridine (ATHP) production, while an inhibitory effect on 2-acetylpyrroline (APY) production by P. parvulus is highlighted when co-inoculated with B. bruxellensis. These findings shed light on the potential impact of the alcoholic fermentation yeast (S. cerevisiae) on the production of mousy off-flavours. Finally, a correlation between ATHP and 2-ethyltetrahydropyridine (ETHP) appears to be confirmed with a ratio of 1:10 between ETHP and ATHP produced in the N-heterocycles assay medium.





Simultaneous assay of mousy off-flavor markers in wine: This is a translation of an article originally written in French.

December 2023

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97 Reads

IVES Technical Reviews vine and wine

The compounds described as being responsible for mousy off-flavor in wine are 2-acetylpyrroline (APY), 2-acetyltetrahydropyridine (ATHP) and 2-ethyltetrahydropyridine (ETHP). However, assay of these compounds is difficult due to their physical-chemical characteristics. The aim of this study was to develop a simple and effective method for simultaneously determining the concentration of these three N-heterocyclic compounds in wine. This new method is a decisive tool for better defining this spoilage phenomenon in wine and guarding against it.


No, Brettanomyces bruxellensis is not responsible for all woes!: This is a translation of an article originally written in French.

December 2023

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77 Reads

IVES Technical Reviews vine and wine

In wines, rice cake and sausage skin aromas are generally associated with the alteration of mousy off-flavours and constitute an increasingly prevalent deviation in some wines. In the present study microorganisms present in 25 altered wines were isolated. The spoilage yeast Brettanomyces bruxellensis, and lactic acid bacteria of the species Oenococcus oeni and Lentilactobacillus hilgardii were identified, and their ability to produce the N-heterocycles responsible for the mousy off-aroma was deterimined. B. bruxellensis was found in a minority of the wines altered by the mousiness.


FIGURE 1. Extract of the experimental procedure for the evaluation of one wine.
FIGURE 2. Boxplots of the valence score distribution (2A) and the arousal score distribution (2B) for the seven wines. The white cross represents the mean score for each wine; the extremities represent the minimum and the maximum values for each wine. Values marked with different letters are significantly different (Tukey post hoc p-value ≤ 0.05).
FIGURE 4. Mean variation (± 5 % confidence interval) of heart rate variation ratio for wines in "Negative valence" (n = 206) and "Positive valence" groups (n = 213).
Is wine an emotional object? Measurements of the subjective and automatic components of emotions in a wine-tasting situation

December 2023

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180 Reads

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3 Citations

OENO One

Wine is often described with emotional terms, such as surprising, disappointing, or pleasant. However, very little has been done to understand the role of emotions in wine tasting and characterise this link between emotions and wine. Many studies have looked at the extrinsic factors that can improve the emotional experience of tasters when discovering a wine, but few have been carried out on the emotional impact of the organoleptic characteristics of wines. The present study aims to determine if the automatic component of emotion has a measurable output (motor and physiological) when tasting wine. If so, does wine tasting induce a concomitant activation of the different components of emotion, such as subjective feelings or physiological and motor responses?Sixty-five connoisseurs tasted seven different red Bordeaux wines with different sensory properties and quality levels pre-defined by wine experts. Emotions were measured using subjective (subjective feelings measurement using self-declarative questionnaires) and automatic (physiological measurements such as skin conductance and heart rate, or motor measurements through facial expressions) methods. The results showed that there was a measurable physiological and motor emotional output in wine tasting. The results also highlighted that changes in the autonomic nervous system in a wine-tasting situation are structured around the dimensions of pleasantness and arousal. Motor measurements taken through facial expressions showed a marginally significant difference between wines providing pleasant and unpleasant emotions for the activation intensity of action units. The relationships established between these components, as well as their concomitant activation, allow us to define wine as an emotional object.


Citations (23)


... Using culture stored at − 80 • C, the strain LO417 isolated from Bordeaux red wine by the Institut des Sciences de la Vigne et du Vin (Bordeaux, France), belonging to a triploid genetic group particularly resistant to SO 2 (Avramova, Cibrario, et al., 2018), producing ethyl-4-phenol andethyl-4-guaiacol (Coulon, Raffestin, Bellan, &Lonvaud-Funel, 2013) and 2-acetyltetrahydropyridine and 2,2-ethyltetrahydropyridine responsible of mousy off-flavours (Moulis et al., 2024) was used. The strain was grown on yeast peptone dextrose (YPD) agar (5 g/L yeast extract, 10 g/L peptone, 20 g/L glucose, and 20 g/L agar supplemented with chloramphenicol at 0.2 g/L to inhibit the development of bacteria) at 28 • C for 6 days. ...

Reference:

Ultra-high irradiance (UHI) blue light treatment: A promising method for inactivation of the wine spoilage yeast Brettanomyces bruxellensis
Impact of Pediococcus parvulus and Saccharomyces cerevisiae on Brettanomyces bruxellensis mousy compound production

OENO One

... Nuclear magnetic resonance, which has commonly been used in food sciences for several decades (Hatzakis, 2019), has gained in popularity in recent years as a tool for wine organic compound screening and analysis (Le Mao, 2023). Another technique that is used is the combination of gas chromatography and mass spectrometry to establish the organic profile (Schartner, 2023). Both methodologies rely on organic component analysis, but because these molecules are sensitive to natural evolution (ageing) or premature changes (oxidation, impacts of storage conditions) to the wine , it is challenging to compare the same sample over time. ...

Predicting Bordeaux red wine origins and vintages from raw gas chromatograms

Communications Chemistry

... 3.3. Diluted to 13.5 °Bé high ACE treatment can produce preferred vegetal smell, and diluted to 13.5 °Bé mod ACE treatment can produce preferred odour intensity Wine aromas will also play an important role in the acceptability of wine and will influence consumer preferences (Sáenz-Navajas et al., 2016;André et al., 2023). For this study, treatment wines without ACE (15.5 °Bé (non-diluted no ACE) and diluted to 13.5 °Bé (no ACE)) have the lowest preference relating to vegetal smell. ...

Sensory dominances depend on the wine quality dimension
  • Citing Article
  • September 2023

Food Quality and Preference

... The quantification was carried out following the internal standard quantification method. The 2-octanol was chosen as internal standard, due to the fact that it did not exist in the analyzed wines, and is used to determine the profile of volatile organic compounds in alcoholic beverages [2,11,18]. The concentration of the identified component was obtained by interpolation of the relative areas of these compounds versus the internal standard area in calibration graphs obtained for reference compounds, similarly to other studies [2,10,11,16,18]. ...

Development of a New Method for the Quantitative Analysis of Aroma Compounds Potentially Related to the Fruity Aroma of Red Wines
  • Citing Article
  • August 2023

Journal of Agricultural and Food Chemistry

... However, concentration at higher level can produce undesirable "buttery" off-flavor. During fermentation, diacetyl is produced by yeast cells in the early stages AF and by LAB in the later stages MLF, resulting in levels of diacetyl that can easily exceed threshold value in wine [1][2][3][4][5][6]. Therefore, adequate control of diacetyl levels is crucial for improving the flavor quality of wine. ...

Sensory Specificities Involving Acetaldehyde and Diacetyl in Wines Produced without Added Sulfur Dioxide
  • Citing Article
  • May 2023

Journal of Agricultural and Food Chemistry

... Eugenol [24] and D-limonene [25] are the main flavor substances in apricot, imparting strong lemon, citrus, and floral-fruity flavors to apricot, while the latter imparts a floral flavor to peaches. The primary process for producing eugenol is the phenylalanine metabolic pathway [26], and monoglucosides are typically found as its precursors [27]. Thus, at the beginning of fermentation, eugenol may be present mainly in its precursor form. ...

Highlighting the varietal origin of eugenol in Armagnac wine spirits from Baco blanc, a hybrid grape variety
  • Citing Article
  • May 2023

Food Chemistry

... and Cladosporium spp. (Abbaszadeh et al., 2014), and inhibition of the patho-gens Botrytis cinerea (Hastoy et al., 2023;Wang et al., 2010), Fusarium oxysporum f. sp. vasinfectum (Abd-Elsalam and Khokhlov, 2015), and Rhizoctonia solani (Zhao et al., 2021). ...

Fungitoxic role of endogenous eugenol in the hybrid grapevine cultivar Baco blanc resistant to Botrytis cinerea

OENO One

... Several strains of B. bruxellensis, have been shown to produce a mousy taint associated with fermenting grape juice, synthetic media, or finished wine (Grbin and Henschke, 2000;Heresztyn, 1986;Moulis et al., 2023;Peynaud and Domercq, 1956;Romano et al., 2008). Their ability to produce ATHP and ETHP and their inability have been confirmed using different chemically defined media (Grbin, 1998;Moulis et al., 2023). ...

Which microorganisms contribute to mousy off-flavour in our wines?

OENO One

... Since SPME Arrow is a new technique, it is not widely used in the analysis of wine volatiles. Lisanti et al. (2021) optimised SPME Arrow extraction conditions for the analysis of compounds contributing to minty aromas in red wines. However, the optimisation process included only nine compounds as targeted analytes, which disenables the usage of the developed method for untargeted analysis and profiling of wine volatiles. ...

Minty aroma compounds in red wine: development of a novel automated HS-SPME-Arrow and gas chromatography-tandem mass spectrometry quantification method
  • Citing Article
  • May 2021

Food Chemistry