Lars Nybo’s research while affiliated with IT University of Copenhagen and other places

We evaluated associations between muscle phenotype, positional role, and on‐ice performance in male U20 Danish national team ice hockey players. Sixteen players (10 forwards, six defensemen) participated in a game with activity tracking. Resting thigh muscle biopsies were analyzed for metabolic enzyme activity and protein expression linked to performance. On‐ice intermittent exercise capacity, repeated sprint ability, and maximal isometric knee‐extensor torque were also assessed. No significant position‐specific muscle phenotype characteristics were found, but forwards generally exhibited higher levels of several membrane proteins (p = 0.100–0.991). NAKα2, NAK∑, KATP, ClC‐1, and NHE1 showed significant correlations with total distance (r = 0.52–0.59, p = 0.016–0.046), however, within positions these only persisted for KATP (r = 0.70, p = 0.024) and NAKα2 (r = 0.57, p = 0.085) in forwards, where CS enzyme activity also displayed a strong association with distance covered (r = 0.75, p = 0.019). For high‐intensity skating, NAKα2 (r = 0.56, p = 0.025) and KATP (r = 0.50, p = 0.048) similarly exhibited the strongest associations, persisting within forwards (r = 0.63, p = 0.052 and r = 0.72; p = 0.018, respectively). In conclusion, although several muscle proteins involved in ion and metabolic regulation were associated with performance, only NAKα2 and KATP displayed consistent relationships within positions. Moreover, CS enzyme activity was strongly related to total distance within forwards, coherent with the proposed importance of oxidative capacity in intense intermittent exercise.

Short-term heat acclimation (HA) appears adequate for maximizing sudomotor adaptations and enhancing thermal resilience in trained athletes. However, for enhanced erythropoiesis and transfer effects to exercise capacity in cooler environments, prolonged HA appears necessary. To establish the time-course for physiological adaptations and performance effects, 20 male elite cyclists were divided into an intervention group (HEAT; n = 10) completing 5 weeks of HA (six one-hour HA-training sessions per week) and control (n = 10) tested pre and post in hot (40°C) and cool conditions (20°C). HEAT completed tests at 40°C every week during HA with measures of sweat rate and [Na+] and a decay test 2 weeks after termination of HA. HEAT improved time for exhaustion by 15 min (p < 0.001) in the 40°C test, increased sweat rate by 0.44 L/hour (p < 0.001),and lowered sweat sodium concentration [Na+] by 14.1 mmol/L (p = 0.006) from pre- to post-HA,with performance returning to pre-HA levels in the 2-week decay test. Total hemoglobin mass(tHbmass) was increased by 30 grams (+3%, p = 0.048) after 3 weeks and 40 grams (+4%, p = 0.038)after 5 weeks in HEAT but returned to pre-HA levels at the 2-week decay test. HEAT improved incremental peak power output (+12 W, p = 0.001) without significant changes in maximal oxygen uptake (p = 0.094). In conclusion, improvements in heat exercise tolerance and sudomotor adaptations materialized during the first ~3 weeks and the entire 5 weeks of HA augmented both cool exercise capacity and tHbmass. However, the 2-week post-HA evaluation demonstrated a rapid decay of physiological adaptations and exercise capacity in the heat-

Fatigue resistance is vital for success in elite road cycling, as repeated, intense efforts challenge the athletes' ability to sustain peak performance throughout prolonged races. The present study combined recurrent performance testing and physiological measures during 6 h simulated racing with laboratory testing to investigate factors influencing fatigue resistance. Twelve male national elite cyclists (25 ± 3 years; 76 ± 6 kg and VO2max of 5.2 ± 0.5 L/min) completed incremental power and maximal fat oxidation tests. Subsequently, they underwent field testing with physiological measures and fatigue responses evaluated through peak sprint power and 5 km time trial (TT) testing after 0, 2, 4, and 6 h of exercise. Peak power declined from 1362 ± 176 W in first sprint to 1271 ± 152 W after 2 h (p < 0.01) and then stabilized. In contrast, TT mean power gradually declined from 412 ± 38 W in the first TT to 384 ± 41 W in the final trial, with individual losses ranging from 2% to 14% and moderately correlated (r² = 0.45) to accumulated exercise time above lactate threshold. High carbohydrate intake (~90 g/h) maintained blood glucose levels, but post‐TT [lactate] decreased from 15.1 ± 2 mM to 7.1 ± 2.3 mM, while fat oxidation increased from 0.7 ± 0.3 g/min at 0 h to 1.1 ± 0.1 g/min after 6 h. The study identifies fatigue patterns in national elite cyclists. Peak sprint power stabilized after an initial impairment from 0 to 2 h, while TT power gradually declined over the 6 h simulated race, with increased differentiation in fatigue responses among athletes.

Introduction Heat-Shield was funded by the European Commission to mitigate the health and productivity effects of occupational heat stress on European workers. Materials and Methods The project involved 20 partners from 11 European countries working together during 2016-2022. Most of these partners still work together to address occupational heat stress in Europe and beyond. Results Heat-Shield research resulted in >75 publications in high-impact journals (Lancet, BMJ, Nature Communications, etc.) that have been cited >2500 times to date. We showed that >100,000 full-time jobs in Europe will be lost by 2030 due to heat-induced health problems and productivity losses. We also showed that the annual cost of occupational heat stress for the EU surpasses €160 billion. Also, we developed and evaluated a series of biophysical solutions and mitigation strategies to address the problem, and we included these in a pan-European heat-health warning system. To disseminate our findings, we co-organized/supported many actions with widespread impact by policy makers and large workers’ unions. Currently, our results serve as the basis for discussions worldwide addressing climate change and occupational health and safety. Our outputs have been adopted by Labour Ministries in their guidance documents and Directives, and have been included in guidance material from international organizations, including the WHO, WHO-Europe, ILO, WMO, and UNFCCC. Conclusions As of September 2023, few EU countries have adopted dedicated policies to protect workers from heat stress. However, there is increasing momentum to adopt relevant EU-wide policies and Heat-Shield has played a key role in this.

Background: Isometric strength testing is widely applied in sports science. However, we hypothesized that traditional testing procedures with a dual focus on both peak force (PF) and rate of force development (RFD) may compromise the true assessment of early RFD measures and lower the associative value towards vertical jump performance. Methods: Therefore, PF and RFD were assessed for 47 active participants (24 females, 23 males) with a traditional isometric midthigh pull (IMTP) protocol ("push as hard and fast as possible" over 4 s) and an RFD-specific protocol ("push as fast as possible" over 2 s). IMTP measures were compared to squat (SJ), countermovement (CMJ) and drop-jump (DJ) performance. Results: The RFD-specific protocol provided higher RFD (P<0.05) for time domains up to 100 ms but lower PF (P<0.001). Independent of protocol, SJ and CMJ performance displayed significant, but low-to-moderate correlations with all RFD measures (r=0.30-0.52) as well as PF (r=0.44), whereas DJ did not show any correlation. Conclusions: In conclusion, an RFD-specific protocol appears relevant for the assessment of RFD in the time domain up to 100 ms. However, the observed associations between RFD/PF measures and vertical jump performance remained low-to-moderate independent of the IMTP test protocol.

This study investigated the impact of a multiday heatwave on nocturnal physiology, behavior, and sleep under controlled conditions with comprehensive monitoring of environmental factors and participant activities. Seven young healthy males were confined for 10 days in controlled conditions that ranged between hot-to-warm (day: 35.4 °C, night: 26.3 °C) during nights 4–6 and temperate (day: 25.4 °C, night: 22.3 °C) before (nights 1–3) and after (nights 7–10) the heatwave. Measurements included core and skin temperatures, heart rate, sympathovagal balance, vasomotion indicators, urine samples, blanket coverage, subjective sleep assessments, and partial polysomnography. The average nocturnal core temperature was 0.2 °C higher during and after the heatwave compared to the pre-heatwave period, with this difference being more pronounced (+0.3 °C) in the first 2 h of sleep (p < 0.001). For every 0.1 °C rise in overnight core temperature, the total sleep time decreased by 14 min (pseudo-R² = 0.26, p = 0.01). The elevated core temperatures occurred despite the participants exhibiting evident thermoregulatory behavior, as they covered 30% less body surface during the heatwave compared to pre- and post-heatwave periods (p < 0.001). During the heatwave, mean skin temperature at bedtime was 1.3 °C higher than pre-heatwave and 0.8 °C higher than post-heatwave periods (p < 0.001). No differences in other responses, including heart rate and vasomotion indicators, were observed. The paper details a 20-min sleepwalking episode that was coupled with marked changes in sleepwalker’s thermophysiological responses. In conclusion, the simulated heatwave resulted in higher overnight core temperature which was associated with reduced total sleep time. Behavioral thermoregulation during sleep may serve as a defense against these effects, though more research is needed.

Exposure to ultraviolet radiation (UVR) leads to skin DNA damage, specifically in the form of cyclobutane pyrimidine dimers, with thymidine dimers being the most common. Quantifying these dimers can indicate the extent of DNA damage resulting from UVR exposure. Here, a new liquid chromatography-mass spectrometry (LC–MS) method was used to quantify thymidine dimers in the urine after a temporary increase in real-life UVR exposure. Healthy Danish volunteers ( n = 27) experienced increased UVR exposure during a winter vacation. Individual exposure, assessed via personally worn electronic UVR dosimeters, revealed a mean exposure level of 32.9 standard erythema doses (SEDs) during the last week of vacation. Morning urine thymidine dimer concentrations were markedly elevated both 1 and 2 days post-vacation, and individual thymidine dimer levels correlated with UVR exposure during the last week of the vacation. The strongest correlation with erythema-weighted personal UVR exposure (Power model, r ² = 0.64, p < 0.001) was observed when both morning urine samples were combined to measure 48-h thymidine dimer excretion, whereas 24-h excretion based on a single sample provided a weaker correlation (Power model, r ² = 0.55, p < 0.001). Sex, age, and skin phototype had no significant effect on these correlations. For the first time, urinary thymidine dimer excretion was quantified by LC–MS to evaluate the effect of a temporary increase in personal UVR exposure in a real-life setting. The high sensitivity to elevated UVR exposure and correlation between urinary excretion and measured SED suggest that this approach may be used to quantify DNA damage and repair and to evaluate photoprevention strategies. Graphical abstract

Performance in intermittent sports relies on the interplay between multiple physiological systems determining the capacity to perform short explosive efforts as well as repeated intense actions with limited recovery over the course of an entire game. Testing should reflect these demands to allow for sport- and position-specific capacity analyses that eventually may translate into optimized training and improved performance. This may include individual load management and optimized training prescription, intensity targeting for specific positions or individual athletes, as well as benchmarking for monitoring of training progression and enhanced engagement of athletes. This review provides an overview of available tests in different exercise domains identified as relevant (from assessment of single explosive actions to intermittent endurance capacity), forming the basis for recommendations on how to compose a comprehensive yet feasible test battery that may be integrated into the seasonal competition and training plan. The test procedures should cover the performance spectrum of relevance for the individual athlete—also in team sports to account for positional differences. We emphasize the benefits of sport-specific tests, highlight parameters of importance for test standardization, and discuss how the applied test battery may be supplemented with secondary tests directed toward specific energy systems to allow for more in-depth analyses when required (e.g., in terms of an underperforming athlete). The synergy between testing and tracking of match performance (utilizing time-motion or global positioning systems) is highlighted, and although tracking cannot substitute for testing, combining the tools may provide a comprehensive overview of the physiological demands and performance during competition contextualized to the athletes’ maximal exercise capacity.

Introduction: This study examined the impact of different upper-torso sportswear technologies on the performance and physiological heat strain of well-trained and national-level athletes during prolonged running in moderately hot conditions. Methods: A randomized crossover design was employed in which 20 well-trained (n = 16) and national-level (n = 4) athletes completed four experimental trials in moderately hot conditions (35°C, 30% relative humidity). In each trial, participants ran at 70% of their peak oxygen uptake (70% V̇O2peak) for 60 min, while wearing a different upper-body garment: cotton t-shirt, t-shirt with sweatwicking fabric, compression t-shirt, and t-shirt with aluminum dots lining the inside of the upper back of the garment. Running speed was adjusted to elicit the predetermined oxygen consumption associated with 70% V̇O2peak. Physiological (core and skin temperatures, total body water loss, and urine specific gravity) and perceptual (thermal comfort and sensation, ratings of perceived exertion, and garment cooling functionality) parameters along with running speed at 70% V̇O2peak were continuously recorded. Results: No significant differences were observed between the four garments for running speed at 70% V̇O2peak, physiological heat strain, and perceptual responses (all p > 0.05). The tested athletes reported larger areas of perceived suboptimal cooling functionality in the cotton t-shirt and the t-shirt with aluminum dots relative to the sweat-wicking and compression t-shirts (d: 0.43–0.52). Conclusion: There were not differences among the tested garments regarding running speed at 70% V̇O2peak, physiological heat strain, and perceptual responses in well-trained and national-level endurance athletes exercising in moderate heat.