L Mannessier's research while affiliated with University of Lille Nord de France and other places

Publications (76)

Article
Despite the generalization of immunoprophylaxis by anti-RH immunoglobulins over 40 years, fetomaternal incompatibility due to RH1 antigen (RhD) is not completely eradicated, although perinatal consequences might be extremely serious. Additionally, allo-immunizations against other antigens, especially anti-RH4 (anti-c) and anti-KEL1 (anti-Kell), may...
Article
Despite the generalization of immunoprophylaxis by anti-RH immunoglobulins over 40 years, fetomaternal incompatibility due to RH1 antigen (RhD) is not completely eradicated, although perinatal consequences might be extremely serious. Additionally, allo-immunizations against other antigens, especially anti-RH4 (anti-c) and anti-KEL1 (anti-Kell), may...
Article
Further to a survey set up in 2006 over 2600 laboratories by the French agency for health product safety (AFSSAPS), seven ABO grouping errors and 53 negative answers to red blood cell antibody screening with a serum containing anti-RH1 antibody, have been found. A questionnaire sent to the involved laboratories revealed non-analytical errors alread...
Article
According to requirements of the French Committee for Accreditation (Cofrac), it is essential to use validated and standardised methods in Immunohematology. This imposes first the knowledge of metrological tolerances for all the technics. Two multicenter studies were carried out to define the maximal acceptable deviations concerning incubation temp...
Article
The French quality control is organized by the French Health Products Safety Agency. In 2005, the immuno-haematology testing control included the screening of an anti KEL 1 antibody. 17 out of 2639 laboratories (0,64%) answered 'negative screening'. All laboratories received a questionnaire in order to understand the failure. In this paper the auth...
Article
According to requirements of the French Committee for Accreditation (Cofrac), it is essential to use validated and standardised methods in Immunohematology. This imposes first the knowledge of metrological tolerances for all the technics. Two multicenter studies were carried out to define the maximal acceptable deviations concerning incubation temp...
Article
Lors de l'opération de 2005 du Contrôle national de qualité portant sur les RAI, 17 laboratoires sur les 2639 participants ont rendu un dépistage faussement négatif. Un questionnaire a été adressé aux laboratoires concernés afin de déterminer la cause de leur erreur. L'analyse de ce questionnaire a fait apparaître, outre la forte implication des bi...
Article
For ten years, the working party of immunohaematology of the French Society of Blood Transfusion organizes a quality control. After the modification of the law about the realization of erythrocyte typing and detection of unexpected red cell allo-antibodies, the quality control was performed in order to determine the sensitivity of the indirect anti...
Article
Le groupe immunohématologie de la Société française de transfusion sanguine organise depuis 15 ans une évaluation externe de la qualité. Elle concerne essentiellement des recherches d'anticorps anti-érythrocytaires. L'évaluation faisant suite à la parution de l'arrêté ministériel en date du 26 avril 2002 (publié au J.O. du 4 mai 2002) modifiant l'a...
Article
The evolution of the methods used in immunohematology is a constant concern for all those involved in the process. Thus, during the last few years, new technologies have been introduced which aim at improving performance. This improvement is not limited to the search for an overall increase in specificity-sensibility; it also takes into account the...
Article
Immunohaematological tests used in antenatal patients have come a long way. However, despite a great deal of progress, we should not loose sight of the fact that these tests give only an indirect measurement and will only help the obstetrician, in conjunction with other fetal parameters, to assess the severity of the haemolytic disease (HD) of the...
Article
The evolution of the methods used in immunohematology is a constant concern for all those involved in the process. Thus, during the last few years, new technologies have been introduced which aim at improving performance. This improvement is not limited to the search for an overall increase in specificity–sensibility; it also takes into account the...
Article
Immunohaematological tests used in antenatal patients have come a long way. However, despite a great deal of progress, we should not loose sight of the fact that these tests give only an indirect measurement and will only help the obstetrician, in conjunction with other fetal parameters, to assess the severity of the haemolytic disease (HD) of the...
Article
ABO blood grouping is a key diagnostic point, which is essential to ensure security of blood transfusions. This analysis must comply with the rules currently in force in our national legislation. It also requires to carry out internal quality checking (IQC) every day. These IQC include the whole process and allow analytical validation of ABO blood...
Article
Cross-matching between the serum of a patient and the red blood cells to be transfused is most important for the prevention of hemolytic transfusion reactions in allo-immunized or new-born patients found positive with direct antiglobulin test. Cross-matching is a time-consuming and complex laboratory test. In order to obtain valid results, it is ne...
Article
Since ten years, the immunohaematology working group of the French Society of Blood Transfusion has organized a quality control. Tests concern essentially the screening and identification of irregular antibodies, direct antiglobulin tests and elutions.
Article
Since ten years, the Immunohaematology working group of the French Society of Blood Transfusion has organized a quality control. Tests concern essentially the screening and identification of irregular antibodies, direct antiglobulin tests and elutions.
Article
Ce travail evalue l'impact de la suppression des reactifs anti-AB et hematies-test A2 sur l'interpretation du groupage sanguin ABO. Il s'est deroule en deux temps : I. Une etude multicentrique concernant l'interpretation de groupages sanguins « tout venants ». Cette etude rapporte les resultats de 105 195 groupages sanguins ABO de donneurs et de pa...
Article
In spite of the progress made since 1970 in specific prevention by anti-rhesus immunoglobulins, and improved management of at-risk pregnancies, allo-immunization due to the erythrocytic Rh 1 antigen (formerly known as Rhesus D or Rh D) remains widespread. In fact, anti-Rh 1 antibodies currently constitute over one-third of the immune antibodies det...
Article
In spite of the progress made since 1970 in specific prevention by anti-rhesus immunoglobulins, and improved management of at-risk pregnancies, allo-immunization due to the erythrocytic Rh 1 antigen (formerly known as Rhesus D or Rh D) remains widespread. In fact, anti-Rh 1 antibodies currently constitute over one-third of the immune antibodies det...
Article
Detection and identification of irregular red-cell antibody in the serum or plasma of a patient is of prime importance for the prevention of hemolytic transfusion reactions and the biological supervision of the hemolytic disease of the foetus or the newborn. Practice in these tests is replete with complex biological problems. Using problem solving...
Article
Detection and identification of irregular red-cell antibody in the serum or plasma of a patient is of prime importance for the prevention of hemolytic transfusion reactions and the biological supervision of the hemolytic disease of the fœtus or the newborn. Practice in these tests is replete with complex biological problems. Using problem solving s...
Article
In a transfusional or foeto-maternal context, hemolysis by incompatibility due to anti-erythrocyte antibodies (regular or irregular) remains the most frequent and most serious immunological risk in the receiver. In order to prevent this risk, a number of actions must be taken, such as the realization of the immunohematologic analyses for which the...
Article
The immunogenic nature of erythrocyte polymorphism is in variance with the incompatible transfusion. Indeed, the fixing of an antibody on the corresponding antigen generally condemns the cell concerned with its destruction. Therefore, in order to ensure the immunohemolytic safety of the transfusions, it is necessary to avoid an in vivo encounter be...
Article
In a transfusional or fœto-maternal context, hemolysis by incompatibility due to anti-erythrocyte antibodies (regular or irregular) remains the most frequent and most serious immunological risk in the receiver. In order to prevent this risk, a number of actions must be taken, such as the realization of the immunohematologic analyses for which the m...
Article
The immunogenic nature of erythrocyte polymorphism is in variance with the incompatible transfusion. Indeed, the fixing of an antibody on the corresponding antigen generally condemns the cell concerned with its destruction. Therefore, in order to ensure the immunohemolytic safety of the transfusions, it is necessary to avoid an in vivo encounter be...
Article
Practice in immunohematology is replete with complex problems that require practitioners' problem-solving performance. In immunohematology, the acquisition of the reasoning process and necessary skills for making clinical decisions is based on teaching problem-solving strategies which potentially reduce errors and improve patient outcome. We discus...
Article
Practice in immunohematology is replete with complex problems that require practitioners' problem-solving performance. In immunohematology, the acquisition of the reasoning process and necessary skills for making clinical decisions is based on teaching problem-solving strategies which potentially reduce errors and improve patient outcome. We discus...
Article
The detection of irregular antibodies is usually performed with serum by the indirect antiglobulin test (IAT) with a polyspecific antiglobulin reagent. In a first study in 1996, we compared the results obtained with 3,264 blood samples of patients drawn with or without anticoagulant: no significant difference was observed among the 240 allo-antibod...
Article
The detection of irregular antibodies is usually performed with serum by the indirect antiglobulin test (IAT) with a polyspecific antiglobulin reagent. In a first study in 1996, we compared the results obtained with 3.264 blood samples of patients drawn with or without anticoagulant: no significant difference was observed among the 240 allo-antibod...
Article
The French Blood Transfusion Society working group Immunohaematology and the French Blood Transfusion Center of Lille performed two quality control exercises-96-01 and 97-02-in order to evaluate identification performances of alloantibodies associated with an autoantibody recognizing a high frequency antigen. Concerning control 96-01, 83 (75%) of t...
Article
The French Blood Transfusion Society working group Immunohaematology and the French Blood Transfusion Center of Lille performed two quality control exercises — 96-01 and 97-02 — in order to evaluate identification performances of alloantibodies associated with an autoantibody recognizing a high frequency antigen. Concerning control 96-01, 83 (75%)...
Article
Prenatal diagnosis of genetic abnormalities requires nucleated fetal cells which are currently obtained by invasive techniques such as amniocentesis, chorionic villus sampling and percutaneous umbilical blood sampling. Each of these entails a risk to the foetus and sometimes to the mother. Nucleated fetal cells have been reported to be present in m...
Article
Many drugs are associated with thrombocytopenic purpura through immune-mediated platelet destruction. The case of a woman who suffered life-threatening thrombocytopenia during vancomycin treatment for Staphylococcus aureus septicemia is reported. Conventional clinical and laboratory methods, including flow cytometry. After treatment of septicemia w...
Article
The Société Française de Transfusion Sanguine and the Centre National de Référence pour les Groupes Sanguins performed a quality control to evaluate the performances of two serological tests: the Direct Antiglobulin Test (DAT) and the Elution test. Among the 110 Blood Transfusion Centers participating in this control, 80 (73%) returned a result. Of...
Article
Blood transfusion is mainly bound to immunological and infectious risks. The immunological risk originates from an incompatibility between the blood of the donor and that of the recipient; this risk remains insufficiently assessed. A multicentre study has been carried out by the French Blood Transfusion Society and the National Institute for Blood...
Article
Alloimmunization to red cell antigens and haemolytic transfusion reactions may occur after red blood cell transfusion. We describe a case of life threatening postransfusion hyperhaemolysis in a β thalassaemia patient. For many years, transfusion therapy was stopped but the patient developed a profound anaemia which required splenectomy. At that tim...
Article
The Société Française de Transfusion Sanguine and the Centre National de Référence pour les Groupes Sanguins performed a quality control to evaluate the performances of two serological tests: the Direct Antiglobulin Test (DAT) and the Elution test. Among the 110 Blood Transfusion Centers participating in this control, 80 (73%) returned a result. Of...
Article
Blood transfusion is mainly bound to immunological and infectious risks. The immunological risk originates from an incompatibility between the blood of the donor and that of the recipient; this risk remains insufficiently assessed. A multicentre study has been carried out by the French Blood Transfusion Society and the National Institute for Blood...
Article
Alloimmunization to red cell antigens and haemolytic transfusion reactions may occur after red blood cell transfusion. We describe a case of life threatening postransfusion hyperhaemolysis in a beta thalassaemia patient. For many years, transfusion therapy was stopped but the patient developed a profound anaemia which required splenectomy. At that...
Article
Vascular endothelial cells are suspected of being the target of autoimmune processes seen in many connective tissue diseases and in systemic vasculitis as evidenced by the detection of circulating autoantibodies against endothelial cell antigens. In order to select B cells recognizing endothelial cells antigens, Epstein-Barr virus (EBV)-infected B...
Article
Full-text available
Donath-Landsteiner hemolytic anemia accounts for one third of all immunologic hemolytic syndromes in pediatric patients. Diagnosis is suggested by results of the direct Coombs test which is positive with anti-C3d, evidence of erythrophagocytosis on admission blood smears, and results of the Donath-Landsteiner test. Anti-P specificity should be rout...
Article
This study reports the results of ABO-Rh (D) typing in microplate according to a suggested protocol. 35,532 blood typings were performed by 13 laboratories, compared to usual technics. This work has proved the feasibility in routine of this protocol in order to identify the A, B, D and weak antigens. However the difficulties in detecting some weak...
Article
Cette étude multicentrique rapporte les résultats du groupage sanguin ABO-Rh (D) en microplaque selon une proposition de protocole. 35 532 groupages sanguins ont été réalisés par 13 laboratoires en comparaison avec les techniques habituellement utilisées. Cette étude prouve la faisabilité en routine de ce protocole pour identifier les antigènes A,...
Article
Peripheral lymphocytes were obtained from an immunized woman against C and Ce antigens. Punction was realized 14 days after childbirth. After hetero-hybridization, a unique cell line continued secreting a monoclonal IgM antibody. Serological characterization of this antibody was determined by direct agglutination tests against 150 native and enzyme...
Article
Peripheral lymphocytes were obtained from an immunized woman against C and Ce antigens. Punction was realized 14 days after child-birth. After heretohybridization, a unique cell line continued secreting a monoclonal IgM antibody. Serological characterization of this antibody was determined by direct agglutination tests against 150 native and enzyme...
Article
This report describes a case of congenital dyserythropoietic anemia (CDA) type I diagnosed in a 22-year-old woman with a two year history of macrocytic anemia. Light microscopy study of bone marrow disclosed only minor and nonspecific findings, without erythroid hyperplasia. Electron microscopy of bone marrow erythroblasts surprisingly showed most...
Article
We report the first occurrence of congenital dyserythropoietic anemia type I in monozygotic twins and the seventh familial occurrence to our knowledge. Mild hemochromatosis is present in the two children but has not yet required iron chelation. Moderate growth retardation, which seems to be related to pituitary failure, is also present.
Article
We report the first occurrence of congenital dyser [(y)\dot]\dot y thropoietic anemia type I in monozygotic twins and the seventh familial occurrence to our knowledge. Mild hemochromatosis is present in the two children but has not yet required iron chelation. Moderate growth retardation, which seems to be related to pituitary failure, is also pres...
Article
Thirty monoclonal anti-Rhesus antibodies were tested : 21 anti-D, 4 anti-E, 4 anti-e and 1 anti-c, ragarding their specificity, avidity, intensity, titer and score. Their type, mode and mean were determined by flow cytometry assay.Then, we tried to determine if the anti-D could adequately dtect Du samples and also studied their behaviour with 13 pa...
Article
We report on a 74-year-old male patient who presented with jaundice, highly increased unconjugated bilirubin and slight nonregenerative anemia. Ferrokinetic studies demonstrated ineffective erythropoiesis and red cell survival, studied by 51 Cr, showed moderately increased hemolysis. Other findings showed no evidence of an underlying myelodysplasti...

Citations

... En cas d'oubli, cette injection peut tout de même être réalisée jusqu'à 30 jours après l'accouchement, selon l'ANAES, pour éviter toute immunisation de la mère. [63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79][80] En France, c'est en 2006 que s'est dégagée un consensus, à l'initiative du CNGOF (Collège National des Gynécologues et Obstétriciens Français) et du CNRHP (Centre National de Référence en Hémobiologie Périnatale), pour recommander l'administration, à 28 SA, d'une dose de 300 µg d'immunoglobuline anti-RH1 chez toutes les patientes rhésus D négatif, en complément de la prophylaxie ciblée. Cette prophylaxie systématique permettrait alors de prévenir les immunisations anti-RH1, dues à des HFM silencieuses du troisième trimestre de grossesse. ...
... La prévention par immunoglobulines anti-D a fait baisser de manière spectaculaire les nouvelles allo-immunisations depuis les années 1970. Néanmoins, ces immunoglobulines, obtenues à partir de plasma humain, et leur utilisation, systématique ou sur signes d'appel, devraient être limitées au minimum pour des raisons de sécurité, d'approvisionnement et de coût [1]. Chez les femmes enceintes RhD-négatif (RH:-1), la connaissance, dès la fin du premier trimestre de la grossesse, du génotypage RHD foetal permet d'éviter les injections d'immunoglobulines inutiles lorsque l'enfant est lui aussi RhD-négatif (RH:-1). ...
... It has the advantages of speed, low reagent consumption, and high-throughput analysis. The U-shaped plate method is fully automated and involves the addition of plasma and RBCs, centrifugation, incubation with constant shaking, and final observation and interpretation (22). The appearance of RBC clots or fine sand in the U-shaped micropores indicates agglutination. ...
... In third position the identification of the antigen and its titration to assess the severity of the immunization; in case of identification of the anti D and for titers greater than 1/32 anti D prophylaxis is recommended. In the presence of maternal genital bleeding, during pregnancy a Kleihauer will be performed to assess the severity of the hemorrhage and the passage of fetal red blood cells into the maternal circulation [5][6][7][8]. ...
... La métrologie est sollicitée lorsque de nouveaux besoins apparaissent : changements règlementaires (spécifications métrologiques), évolutions techniques (nouveaux équipements). Malgré tout, bien que quelques études aient déjà été publiées[7,8], les équipes de métrologie sont paradoxalement peu concertées dans le cadre de la validation de méthodes, alors que ces dernières sont parfois à l'origine de dépôts de brevets et de publications. Pourtant, la robustesse et la reproductibilité des travaux dépendent en grande partie de la caractérisation métrologique des procédés. ...
... In environments where QM is "forced," the emphasis has often been placed on quality assurance, with frameworks such as accreditation standards, registration and licensing, or peer review. [2][3][4][5][6][7] Organizations choosing QM have sometimes used some of the above models, 8 but have also used quality award frameworks such as EFQM in Europe or Baldrige in the United States, 5,[9][10][11][12] QM systems such as ISO 9001, [13][14][15] methods such as reengineering, [16][17][18] Six Sigma, 15,19,20 benchmarking, [21][22][23] PDSA, and the rapid improvement cycle. 24,25 Others have chosen to seek clinical improvement through evidence-based medicine 26,27 or through quality collaboratives. ...
... Le mélange réactionnel hématies-plasma est incubé à 37 °C, qui est l'optimum thermique des IgG [24], principale classe d'immunoglobuline retrouvée parmi les allo-anticorps d'intérêt clinique. Les « réaction + » et « réaction -» dénotent respectivement la présence ou l'absence d'allo-anticorps anti-érythrocytaires. L'antiglobuline humaine (AGH), polyvalente (anti-IgG+anti-C3d) ou spécifique anti-IgG [18], permet de révéler la réaction antigène-anticorps. L'AGH utilisée en microfiltration permet de détecter des anticorps de classes IgG et IgM, alors que celle utilisée en microplaque ne détecte que des IgG ; l'impact clinique résultant est mal caractérisé à ce jour. ...
... Il permet une approche de la concentration réelle en IgG anti-RH dans le sérum maternel. Le seuil dangereux est de 1μg/ml [10]. ...
... La RAI comporte une première étape de dépistage, avec une gamme de 3 hématies-tests de groupe O comportant des antigènes et des phénotypes obligatoires avec expression phénotypique homozygote respectée pour certains antigènes, en présence du sérum ou du plasma du patient, selon le principe du test indirect à l'antiglobuline (antiglobuline polyspécifique ou anti-IgG), en milieu de basse force ionique, en technique de gel de filtration ou immunoadhérence [1]. ...
... Pour la validation, des modèles viraux sont utilisés conformément aux exigences des autorités compétentes. On retrouvera alors : III/ Réglementation régie par le code la santé publique (Articles R5144-25 à R5144-34) [129][130][131][132][133] La pharmacovigilance exercée sur les médicaments dérivés du sang comporte un suivi, appelé traçabilité, effectué depuis leur fabrication jusqu'à leur administration aux patients. ...