L C Teh's research while affiliated with Garvan Institute of Medical Research and other places

Publications (5)

Article
The distribution of 125I radioactivity in the liver, kidneys, adrenals and serum of male rats was measured 10 minutes after an intravenous bolus of 125I-labelled human growth hormone (hGH) was administered in the presence or absence of a large excess of ovine growth hormone or ovine prolactin. The hGH binding sites in the adrenals had displacement...
Article
A technique is described to study the effect of acetylation of individual lysine residues in peptide hormones on the affinity for their receptors, and is illustrated for the case of human growth hormone (hGH) binding to somatogenic receptors. The hGH was partially acetylated with high specific activity [3H]-acetic anhydride and the product ([3H]-Ac...
Article
Full-text available
The oxidation of the methionine residues of human growth hormone (hGH) and human chorionic somatomammotropin (hCS) to methionine sulfoxide by hydrogen peroxide has been studied. The kinetics of oxidation of individual methionine residues has been measured by reverse-phase high pressure liquid chromatography tryptic peptide mapping. Met-170 is compl...
Article
Osteocalcin (the 6,000 dalton Mr gamma-carboxyglutamate-containing protein of bone) has been detected in acid extracts of bones of the extinct class of New Zealand ratite birds, the moas, using a radioimmunoassay for sheep osteocalcin. The immunoreactive osteocalcin of the extracts of two of these bones (the fibulae from two specimens of Pachyornis...
Article
The amino acid sequences of osteocalcins from cortical long bones of goat, pig and wallaby have been determined by manual microsequencing methods. The location of the gamma-carboxyglutamic acid residues were identified by N-terminal degradation of peptides in which these residues had been tritium-labelled in the gamma-H position and subsequently th...

Citations

... Different concentrations of unlabeled caprine and human GH (0, 0.1, 1, 10, 100 and 1000 μg) were used in the assay and results showed that 1000 μg concentration of both unlabeled hormones disrupted the binding of the 125 I-rcGH at a maximum level. These results were in consensus with that of Tech et al. (1988). The cross reactive results explained that human GH binds with high affinity to the Bovidae microsomal membrane or with non-primates GHR and similar results have also been reported by Souza et al. (1995). ...
... The oxidation of methionine to the methionine sulfoxide form has been widely reported for many types of proteins [12][13][14][15]. Although methionine oxidation has been reported to be reversible in vivo through the activity of the methionine sulfoxide reductase (MsR), which catalyzes the thioredoxin-dependent reduction of MetO back to Met, it is not yet clear whether, in the context of therapeutic mAbs, an oxidized methionine can be readily reduced back to Met [11,[16][17][18]. ...
... [1,3,[10][11][12][13] Additionally, because of its close association with hydroxyapatite, size, and acidity, it has been hypothesized to persist into the deep time making it a prime target for paleontological and archaeological investigations. [14][15][16][17][18][19][20][21][22][23][24][25] It has also been a target for fossil remains because the N-terminal residues are variable providing greater phylogenetically informative sequences than other commonly preserved proteins in bone (e.g., collagen I). [18] It is a small protein (~5800 Da) with three vitamin K dependent γ-carboxylated glutamic acid residues (Gla) at positions 17, 21, and 24 [26] that allow for direct binding to calcium in hydroxyapatite. ...
... The positions of hydroxyproline residues are indicated by P and the positions of gammacarboxyglutamic acid (Gla) residues are indicated by E. T: tryptic peptides of bovine osteocalcin, AP: peptides generated by cleavage with 70% formic acid, S: synthetic peptide. Source of osteocalcin sequences: cow (1), man (22), pig (23), sheep(24), goat(23), rat(25), and chicken(26). ...