L A Abreu’s research while affiliated with Oswaldo Cruz Foundation and other places

High levels of serum ceruloplasmin oxidase activity were detected in U strain isogenic male rats bearing a syngeneic sarcoma in all phases of tumoral evolution. Normal levels of ceruloplasmin were only observed after a single i.p. dose of cyclophosphamide (100 mg/kg). However subsequent weekly administration of the drug did not prevent the return of hyperceruloplasminemia in the tumor hosts. Normal rats treated similarly with cyclophosphamide presented a fall in the enzyme activity after the 1st dose.

Ehrlich ascites carcinoma induced significant changes in liver fumarase (activation) and aconitase (inhibition). A significant increase in hepatic fumarase specific activity was also observed in mice inoculated with Ehrlich ascites fluid after centrifugation of cells (EAF). Hepatic aconitase was not significantly influenced by EAF.

Serum ceruloplasmin oxidase activity did not change in mice treated orally for 120 days with Li2CO3 (0.58 mEq/kg/day). After a single i.p. injection of LiCl (20 mEq/kg), a significant activation of ceruloplasmin was observed.

Résumé Nous avons déterminé l'activité spécifique de l'aconitase cérébrale des souris après une période de 108 jours de traitement avec du lithium (Li2CO3). Une inhibition significative de l'enzyme à été observée. D'autre part, nous n'avons trouvé in vitro aucun effet du Li2CO3 sur l'aconitase cérébrale.

Résumé On montre que la présence du carcinome d'Ehrlich provoque une diminution statistiquement significative des taux de la céruloplasmine sérique des souris aux dixième et quatorzième jours après l'inoculation.