Kyle Roessler’s research while affiliated with Salish Kootenai College and other places

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Publications (1)

Base media added solutes/metals KCl, MgSO4, CaSO4, and NaSO4. (A) H. anomala grew at nearly the same rate as no added metal in the presence of KCl. (B) Growth of the organism was slowed by approximately half with the addition of MgSO4. (C) CaSO4 was within the error rate for matching growth with no added metal. (D) NaSO4 appeared to increase H. anomala growth in relationship to no added solute (metal).
Base media added solutes/metals AlSO4, FeSO4, ZnSO4, MnSO4, and CuSO4. (A) H. anomala growth in base media. (B) No observed growth with added AlSO4. (C) H. anomala exhibited no growth when FeSO4 was added to base media. (D) No measurable growth was recorded with the addition of ZnSO4 to base media. (E) Very little measurable growth was observed with the addition of MnSO4 to base media at 72 h post inoculation. (F) Some growth was recorded for CuSO4 additive media at 72 h post inoculation.
Base media with elemental S and added solutes/metals KCl, MgSO4, CaSO4, and NaSO4. (A) H. anomala had increased growth with KCl and elemental S than without S. (B and C) Organism growth was nearly as high as base media and S with added MgSO4 and CaSO4. (D) Growth with NaSO4 and elemental S was suppressed.
Base media with elemental S and added solutes/metals AlSO4, FeSO4, ZnSO4, MnSO4, and CuSO4. (A) H. anomala growth in base media with elemental S. (B) AlSO4 in base media with added elemental S had negligible growth. (C) FeSO4 suppressed growth in base media, but had significant growth with added elemental S. (D) Organism growth was negligible with added elemental S and ZnSO4. (E) H. anomala growth with MnSO4 showed some minimal growth at 48 h with the added elemental S that declined slightly at 72 h. (F) Similar to CuSO4 in base media, added elemental S did not rescue H. anomala growth.
Hanensula anomala isolated from the Berkeley Pit, Butte, MT, is a metal-specific extremophile
  • Article
  • Full-text available

August 2024

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12 Reads

Microbiology Spectrum

Kyle Roessler

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Mariah C. Friedlander

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Marthe Y. VanSickle

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Christina L. Rush

A yeast-like extremophile organism, Hansenula anomala, has been isolated from the superfund site the Berkeley Pit Lake in Butte, Montana. Studies demonstrate H. anomala growth in some of the known Berkeley Pit Lake solutes. Microbial growth dynamics under controlled conditions were compared of H. anomala for multiple metal concentrations. Each solute/metal was tested separately at previously reported concentrations on the geochemistry of the Berkeley Pit lake in the first 0.2 m in spring (pH 2.5). H. anomala grew well with sulfur (S), MgSO4, CaSO4, potassium chloride (KCl), and NaSO4 and was inhibited with FeSO4, MnSO4, CuSO4, AlSO4, or ZnSO4. With the addition of elemental S, growth was observed for FeSO4 indicating minimal growth rescue. PCR amplification of genomic DNA from the organism using known ribosomal primers indicates the strain to be ATCC8168 (CBS 5759). From this data, it can be concluded that H. anomala ATCC8168 from the Berkeley Pit is an extremophile that exhibits metal-specific growth. IMPORTANCE Laboratory growth studies of a strain of Hansenula anomala from the Berkeley Pit have found the organism to be metal specific indicating some unique metabolism possibilities. These studies show that this strain is metal-dependent and provides information about the adaptable tolerance of organisms in superfund sites as well as giving a basis for future bioremediation development utilizing H. anomala.

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