Kuntal Manna’s research while affiliated with Tripura University and other places

The purpose of the present study is to evaluate the antitumor activity (ATA) of traditional herbal preparation Sivanar Amirtham (SA) on Dalton's Lymphoma Ascites. Siddha medicine system (SMS) is a traditional system of medicine originated from ancient Tamilakam of South India. Siddha medicine is a traditional healing system from Tamilakam in ancient South India. For our purpose, we have performed acute toxicity (AT) study as per OECD guidelines 423 and ATA by xenograft method. In this study, a single dose of 300, 1000 and 2000 mg/kg of Sivanar Amirtham suspension (SAS) was orally (p.o.) administered in mice and animals were observed for 14 days. For antitumor study (ATS), we have used DAL cells which were intraperitoneally (i.p.) inoculateded into mouse. The ATAs were studied by monitoring the parameters such as cell growth inhibitors, tumor weight measurements, mean survival time of DAL bearing mice as well as changes in depleted haematological and biochemical parameters due to tumorigenesis. The SAS was also evaluated for in vitro cytotoxicity study in different concentration and the viability of cells was determined by exclusion method of trypan blue dye (TBD). The AT study showed no signs of toxicity and no mortality after single administration of SAS. SAS caused significant decrease in packed cell volume (PCV) (value), Tm volume (value) and viable cell count (value), and it prolonged the life span of DAL Tm carrying mice. Haematological and biochemical profiles were reverted to normal levels in SAS treated mice. The results of in vitro cytotoxicity show that SAS showed significant ATA in mice with moderate DAL levels. The IC50 value turned into discovered to be 800 μg/ml from the in vitro cytotoxicity examine. The study strongly suggests that SAS has the potential to be an antitumor medication against DAL cells induced Tm and it can be extrapolated for further cancer (CA) prevention applications.

Diplazium esculentum belongs to the family Athyriaceae, an edible fern rich in micronutrients, beta-carotene, folic acid, and minerals like calcium, iron, and phosphorus. The present study aims to determine secondary metabolites and minerals content and evaluate the antioxidant and antidiabetic activities of young fronds of Diplazium esculentum. The plant extract was prepared by the Soxhlet apparatus method. Total alkaloids and total tannin content were determined by the titrimetric method. Folin and Ciocalteu's colorimetric method used gallic acid as a standard drug to determine total phenols. The antioxidant activity of plant extract was evaluated by DPPH free radical scavenging and hydrogen peroxide scavenging assay. α-amylase inhibition assay was performed for evaluation of the antidiabetic activity of plant extract. Fronds of Diplazium esculentum contain a significant number of total alkaloids (145.54±1.49), phenols (174.2±0.95), and tannins (263.96±1.32) mg/g of dry extract. Fronds of Diplazium esculentum also contain a significant amount of minerals. Diplazium esculentum shows significant antioxidant activity compared to the standard drug ascorbic acid and antidiabetic activity compared to the standard drug metformin. Based on these findings, young fronds of Diplazium esculentum can be recommended as a good source of phytochemicals and minerals. It also shows significant antioxidant and antidiabetic activities.

Alkaloids are waste products of plant metabolic processes, containing at least one nitrogen atom in the heterocyclic ring. They serve a wide variety of physiological functions in humans and animals. Metabolism plays a central role in regulating the toxicity of various phytochemicals. Available literature of biological sources and metabolism study of pyrrolidine, pyrrolizidine, pyridine, quinoline, isoquinoline, phenanthrene, phenethylamine, indole, terpenoid, and aporphine groups of experimental plants-derived alkaloids were collected from the Google Scholar, PubMed/Medline, Science Direct, Scopus, Wiley Online Library, and Web of Science search engines. The literature reveals that hepatic microsomal enzymes such as monooxygenase and putative NADPH-FMN-reductase, carboxyl esterase, CYP2B6, CYP3A4, and CYP2D6 are primarily involved in the metabolism of alkaloids. This review may encourage natural product researchers to further research and develop new plant-derived alkaloidal drugs.

Traditional medicine may also cause genetic damage that may increase the possibility of cancer and other diseases. Therefore, it is essential to evaluate the genotoxic testing of Siddha drugs. Therefore, a latest take a look at become performed to evaluate the in vivo genotoxic effects of Sivanar Amirtham in mice using micro nucleus assay. Three different doses of 100, 200, and 400 mg / kg b.w were selected in accordance with OECD guidelines. In a micronucleus test, the administration of one oral Sivanar Amirtham at 100, 200 and 400 mg / kg b.w did no longer increase with the mean amount of micronucleated polychromatic erythrocytes or PCE percentage for both sexes showing non clastogenicity. For this reason it could be concluded, Sivanar Amirtham showed no vast genotoxic impact on the rats in both sexes. Sivanar Amirtham was therefore not carcinogenic and therefore could be used safely.

Nutraceuticals are food-based drugs that are used as dietary supplements to minimize chronic diseases. Diabetes is one of the most common chronic diseases all over the world. Recently, herbal nutraceuticals have taken a promising role in treating diabetes. We aimed to develop herbal nutraceutical tablets and evaluate its anti-diabetic activity using ob/ob mice. Five plant species were collected by field survey methods based on oral interviews with traditional healers of Tripura. The wet granulation method was applied to formulate the herbal nutraceutical tablet. Water- and fat-soluble vitamins were determined by reversed-phase highperformance liquid chromatography. Trace elements were analyzed by atomic absorption spectrophotometry. To evaluate the anti-diabetic activity of the herbal tablets, we determined serum hemoglobin, glycosylated serum protein, and oral glucose tolerance. The newly formulated herbal nutraceutical tablets provided the optimal energy level. It contained sufficient amounts of essential minerals, such as iron (74.6 ± 2.7 mg/g), sodium (4.4 ± 0.4 mg/g), potassium (5.3 ± 0.7 mg/g), calcium (163.1 ± 2.2 mg/g), magnesium (39.2 ± 1.7 mg/g), and phosphorus (14.6 ± 2.1 mg/g). We also found optimal quantities of water-soluble vitamins, such as vitamin C (27.2 ± 4.3 mg/g), vitamin B1 (0.6 ± 0 mg/g), vitamin B3 (0.6 ± 0.2 mg/g), vitamin B6 (1.1 ± 0.2 mg/g), vitamin B12 (0.6 ± 0.2 μg/g), and folic acid (82.6 ± 7.6 μg/g), as well as fat-soluble vitamins, such as vitamin A (287.4 ± 6.3 μg/g), vitamin D3 (2.6 ± 0.6 μg/g), and vitamin E (0.7 ± 0 ng/g). Finally, the herbal nutraceutical tablet (200 mg/kg) significantly improved the antihyperglycemic effect on ob/ob mice (type 2 diabetes), compared to the standard drug, metformin (200 mg/kg). The results suggest that the newly formulated herbal tablet may be recommended as an anti-diabetic nutraceutical drug.

A novel heterocyclic hybrid dihydropyrazol-1-yl-2-(quinolin-8-yloxy) derivatives were synthesized. By reacting 8-hydroxyquinoline 1 in absolute ethanol with potassium carbonate followed ethyl chloroacetate by conventional and microwave irradiation methods, precursor 2 were synthesized, further treated with hydrazine hydrates to achieve precursors 3 and by Claisen-Schmidt condensation methods, different chalcone derivatives 6(a-g) were prepared and to these precursors 3 was then cyclized to give the target compounds 7(a-g). The structures of the target compounds were evaluated by the spectral and elemental method of analysis. The screened compounds 7a and 7g produced better action than other compounds but somewhat lesser than the standard drugs. Furthermore molecular docking studies have been carried out against PDB codes: 5MTX (p38 MAPK) using Biovia Discovery Studio 2021. The compounds 7a (-8.947 Kcal/mol) and 7g (-8.994 Kcal/mol) had shown remarkable binding affinities which were greater than the binding affinity of recommended drugs; diclofenac sodium (-6.8 kcal/mol), and nimesulide (-7.8 kcal/mol).

Review Article

Amoebiasis is an infectious disease caused by Entamoeba histolytica (E. histolytica), the common symptoms are cramping, abdominal pain, watery or bloody diarrhea, and weight loss. Sometimes patients suffer amoebiasis as an asymptomatic behavior. In 1859 scientist W.D. Lambl first described the amoebiasis vector E. histolytica. In developing countries, millions of people die due to amoebiasis. A doctor diagnosed the disease detection of E. histolytic antigen in stool or antibodies against the parasite in serum. Metronidazole, tinidazole, lodoquinol, diloxanide furoate are given for amoebiasis patients. In this review, we have summarized the statistics, pathogenesis, diagnosis, prevention, and treatment strategy of amoebiasis disease. It also gives information about the life cycle of E. histolytica.