Kuber T. Sampath's research while affiliated with Stryker Corporation and other places

Publications (14)

Article
Full-text available
PurposeOsteogrow, an osteoinductive device containing recombinant human Bone Morphogenetic Protein 6 (rhBMP6) in autologous blood coagulum, is a novel therapeutic solution for bone regeneration. This study aimed to evaluate different commercially available calcium phosphate synthetic ceramic particles as a compression-resistant matrix (CRM) added t...
Article
Full-text available
Bone morphogenetic proteins (BMPs) are potent osteoinductive agents for bone tissue engineering. In order to define optimal properties of a novel autologous bone graft substitute (ABGS) containing rhBMP6 within the autologous blood coagulum (ABC) and ceramic particles as a compression resistant matrix (CRM), we explored the influence of their amoun...
Article
BACKGROUND CONTEXT RhBMP2 soaked in bovine tendon collagen and synthetic ceramics composite as well as rhBMP7/OP1 lyophilized in bovine bone collagen particles with additive carboxyl-methyl cellulose have been evaluated for posterolateral lumbar fusion (PLF). We investigated rhBMP6 delivered in autologous blood coagulum (ABC), a native carrier (sca...
Chapter
BMPs are originally identified based on their ability to induce new bone in vivo and represent large members of the TGF-β superfamily of proteins. BMPs serve as inductive signals for cell migration, growth, and subsequently differentiation in many organ developments during embryogenesis and are shown to modulate inflammation, angiogenesis, and immu...
Chapter
Bone morphogenetic protein (BMP)-7 is required for embryonic kidney development, plays a functional role in the adult kidney as renal hormone for vascular and skeletal integrity, and modulates calcium and phosphate homeostasis. Preclinical studies have shown that systemic administration of recombinant BMP-7 provides tissue protection in models of a...
Chapter
The bone morphogenetic proteins (BMPs) are growth and differentiation factors and form a large family of proteins structurally related to TGF-βs and activins. BMP-2 and BMP-7 containing osteogenic devices (InFuse® and OP-1®, respectively) have been used as bone graft substitutes for the repair of long-bone fractures and anterior lumbar interbody an...
Patent
Full-text available
Provided herein are methods and devices for inducing the formation of functional replacement nonarticular cartilage tissues and ligament tissues. These methods and devices involve the use of osteogenic proteins, and are useful in repairing defects in the larynx, trachea, interarticular menisci intervertebral discs, ear, nose, ribs and other fibroca...
Book
Tissue engineering is gaining interest as it is applied for regeneration of organs to attain their lost function. Although resorbable scaffolds and progenitor cell types are required principles to engineer a functional tissue locally, the inductive signal is a prerequisite to trigger the growth and differentiation of responding cells in space and t...
Book
Since the discovery of the bone morphogenetic proteins (BMPs) more than 15 years ago, there has been an unpredicted explosion of both basic scientific discoveries and clinical reports on their use from institutions all over the world. The potent effica­ cy of BMPs in almost all crucial developmental events as well as during regenera­ tion of variou...
Chapter
Bone regenerates itself upon fracture by instituting a cellular process that mirrors embryonic bone development and restores function fully following its usage. Demineralized bone matrix (DBM) has been widely used as a bone graft substitute to induce new bone formation during reconstructive surgery as it reproduces the embryonic cellular events tha...
Book
From the basic science to potential and approved clinical applications the most recent data in the rapidly growing field of bone morphogenetic proteins (BMPs) are summarized in this topical volume. Distinguished scientists present reviews on a range of scientific topics, including biochemistry, biology, molecular biology and preclinical animal stud...
Chapter
Members of TGF-β superfamily are secreted glycoproteins and have been shown to regulate biological processes as diverse as migration, proliferation and differentiation of pluripotent progenitor cells involved in the development of several organ systems during embryogenesis and in adult tissue repair [1, 2]. The kidney has been identified as a major...

Citations

... To improve the biomechanical properties of Osteogrow implants might be supplemented with allograft bone (Osteogrow A), host bone (Osteogrow B) and synthetic ceramics (Osteogrow C) (144)(145)(146). Osteogrow family of products have been proven safe and efficacious in rat subcutaneous assay, rabbit segmental defect model as well as rabbit and sheep posterolateral spinal fusion (PLF) model (144,145,(147)(148)(149)(150)(151)(152)(153)(154)(155). Moreover, Osteogrow has been evaluated in the phase 1 study in patients with distal radius fractures (156), in phase 2 in patients with high tibial osteotomy (157) and in phase 2 in patients treated by posterolateral lumbar interbody fusion for degenerative disc disease. ...
... To improve the biomechanical properties of Osteogrow implants might be supplemented with allograft bone (Osteogrow A), host bone (Osteogrow B) and synthetic ceramics (Osteogrow C) (144)(145)(146). Osteogrow family of products have been proven safe and efficacious in rat subcutaneous assay, rabbit segmental defect model as well as rabbit and sheep posterolateral spinal fusion (PLF) model (144,145,(147)(148)(149)(150)(151)(152)(153)(154)(155). Moreover, Osteogrow has been evaluated in the phase 1 study in patients with distal radius fractures (156), in phase 2 in patients with high tibial osteotomy (157) and in phase 2 in patients treated by posterolateral lumbar interbody fusion for degenerative disc disease. ...
... Bone morphogenetic proteins (BMPs) are a large group of growth and differentiation factors that belong to the transforming growth factor-beta (TGF-β) protein superfamily. They have pleiotropic effects in numerous tissues during embryogenesis but also exhibit a prominent role in the regulation of physiological processes and final anatomy of the specimen during postnatal growth and development [1]. All BMP members elicit their cellular effects by ligand-induced activation of type I and II transmembrane serine/threonine kinase receptors. ...
... Recent studies demonstrate the feasibility of enhancing implant osseointegration and gap bridging by covalently immobilized rh-BMP-2 on titanium and polymer implant surfaces [29,30]. The described grafting increases the half-life of rh-BMP-2 up to 40-60 days (half-life of rh-BMP-2 is approximately 2.5 days in vivo [31]) and allows controlling the sustained release of rh-BMP-2 with a high level of biological activity. Mercado et al. provided evidence that rh-BMP-2 grafted to biodegradable polymer nanoparticles was, after 14 days, as effective in inducing mineralization as the native rh-BMP-2 [30]. ...
... They provide morphogenetic signals for skeletal development during embryogenesis and are responsible for adult fracture healing by recapitulating a cascade of cellular events associated with embryonic bone formation. More than 30 different BMPs based on structural similarity have been identified and some of them were suggested to play a role beyond bone [9][10][11]. Bone-inducing BMPs are divided into several subgroups, based on the homology of their amino acid sequences: BMP2/BMP4, BMP5/BMP6/BMP7, BMP9/BMP10, and BMP12/BMP13/BMP14 groups, while other BMPs do not have proven osteogenic properties [10]. ...
... BMPs are secreted signaling molecules which belong to the large protein family consisting of more than 30 ligands, called transforming growth factor-b (TGFb) superfamily (7,8) and comprise an evolutionary conserved family of cytokines required for numerous developmental processes. Among the TGFb superfamily members, the bone-formation activity is unique to BMPs (9); however, it was shown that BMPs have many other biological activities (10). Since their isolation as promotors of bone and cartilage formation, BMPs have been extensively studied and, besides their confirmed role in bone and cartilage, have been found to hold multiple functions in the embryonic development of other tissues and organ systems, including blood vessels, brain, liver, heart, lung, gut, limb, eye, teeth, or kidney. ...
... In animal studies, bone morphogenetic proteins (BMPs) and growth/differentiation factors (GDFs) have been used for the surgical filling of traumatic or tumor non-loaded bone defects [5][6][7] and the spinal fusion of acute vertebral body fractures [8,9]. rhBMP2 (InFUSE ® ; Amplify ® ) is licensed under a premarket approval (PMA) for marketing and clinical use in the United States and has been developed as a product for the repair of long bone non-union fractures and lumbar spinal fusion in humans ( [10] and references therein). However, clinically approved high-dose BMP application (3.5 to 12 mg; [11,12] and references therein) results in an initial burst release believed to be responsible for inflammatory and osteoclastic side effects, and may contribute to ectopic bone formation, potential risk of osteosarcoma and autoantibody formation, or spinal complications [13][14][15]. ...
... As an alternative to exogenous materials with their requisite shortcomings, a more sophisticated understanding of craniofacial biology has, in recent years, allowed for the evolution of diverse strategies in craniofacial reconstruction based on the premise of inducing the generation of autogenous bone: "generative" craniofacial surgery. A great deal of this work takes its origins from the discovery of the bone morphogenetic proteins (BMPs) by Marshal Urist in the 1960s, 2 and the eventual isolation of BMP-2 as a protein capable of stimulating bone growth by intramembranous and endochondral ossification by Wozney et al. and Celeste et al. [3][4][5][6][7] Tissue engineering involves three key elements: cells that constitute and produce the tissue of interest, proteins that mediate the processes involved in tissue regeneration, and scaffolds that provide a structural framework. In a previous study, we reported success using recombinant human (rh) BMP-2 delivered by means of an acellular collagen sponge for the repair of large-scale calvarial defects in a New Zealand White rabbit model. ...