Kristýna Kotýnková's research while affiliated with Boston University and other places

Publications (13)

Article
Full-text available
Melanoma is commonly driven by activating mutations in the MAP kinase BRAF; however, oncogenic BRAF alone is insufficient to promote melanomagenesis. Instead, its expression induces a transient proliferative burst that ultimately ceases with the development of benign nevi comprised of growth-arrested melanocytes. The tumor suppressive mechanisms th...
Article
Full-text available
Whole-genome doubling (WGD) is common in human cancers, occurring early in tumorigenesis and generating genetically unstable tetraploid cells that fuel tumour development1,2. Cells that undergo WGD (WGD+ cells) must adapt to accommodate their abnormal tetraploid state; however, the nature of these adaptations, and whether they confer vulnerabilitie...
Preprint
Full-text available
Whole genome doubling (WGD) occurs early in tumorigenesis and generates genetically unstable tetraploid cells that fuel tumor development. Cells that undergo WGD (WGD+) must adapt to accommodate their abnormal tetraploid state; however, the nature of these adaptations, and whether they confer vulnerabilities that can subsequently be exploited thera...
Data
Movie S6. Live-Cell Imaging of MyrPalm-GFP Localization during Cytokinesis, Related to Figure 6D
Data
Movie S4. Live-Cell Imaging of Cry2-mCh-ECT2 with Unilateral Blue-Light Illumination, Related to Figure 4E
Data
Movie S1. Live-Cell Imaging of ECT2-C1B and ECT2-C1BQ27G after TPA Treatment, Related to Figure 1C
Data
Movie S5. Live-Cell Imaging of ECT2-WT and ECT2-BRCTTK Localization during Cytokinesis, Related to Figure 6C
Data
Movie S2. Live-Cell Imaging of AcFL-C1B and AcFL-C1BQ27G Proteins after TPA Treatment, Related to Figure 1C and Figures S1D and S1E
Data
Movie S3. Live-Cell Imaging of Cry2-mCh-ECT2 with or without Blue-Light Illumination, Related to Figure 4C
Article
Full-text available
Cytokinesis, the final step of cell division, begins with the formation of a cleavage furrow. How the mitotic spindle specifies the furrow at the equator in animal cells remains unknown. Current models propose that the concentration of the RhoGEF ECT2 at the spindle midzone and the equatorial plasma membrane directs furrow formation. Using chemical...

Citations

... Indeed, inhibition of both genes has been previously suggested to induce cell lethality by generating excessive CIN 44 . CX9 was correlated with perturbation of BUB1B, a spindle assembly checkpoint gene recently identified as therapeutically relevant in CIN-high cells measured via WGD status 45 and an aneuploidy score 7 . This association with CX9 suggests that the spindle assembly checkpoint may have a crucial role in tolerating mid-level amplifications, and reducing levels of BUB1B may induce excessive and catastrophic chromosome missegregation 46 . ...
... For instance, cell cycle and mitotic progression were not perturbed in tetraploid yeast cells (Storchová et al., 2006), which may be due to the lack of spindle scaling in those strains. In contrast, tetraploid mammalian cells often progress through mitosis more slowly than their diploid counterparts (Kuznetsova et al., 2015;Paim and FitzHarris, 2019;Cohen-Sharir et al., 2020;Quinton et al., 2020), suggesting a possible delay in SAC silencing. Several recent studies, which explored the link between cell and spindle sizes and SAC function more closely (Gerhold et al., 2015(Gerhold et al., , 2018Galli and Morgan, 2016;Kyogoku and Kitajima, 2017), also produced conflicting results. ...
... Recruitment of PI(4)P-containing vesicles at ERmitochondria contact site and promotion of mitochondrial fission [152] Miro Miro1 and Miro2 bind CENP-F and associate with microtubule-growing tips. Miro loss decreases the spreading of the mitochondrial network and causes cytokinesis-specific defects [154] Midbody RhoA Position of ingression furrow and membrane ingression and abscission [161,[169][170][171][172]175,176] Rac1 Membrane ingression. The overexpression of constitutively active Rac1 causes multinucleation and cytokinesis failure [42,187,192] Rab1 Rab1 facilitates new membrane supplementation along the ingressing cleavage furrow [191] Rab11 ...