Kristy S McKeating's research while affiliated with University of California, Riverside and other places

Publications (16)

Article
An important advance in biosensor research is the extension and application of lab-developed methodologies toward clinical diagnostics, though the propensity towards non-specific binding of materials in clinically relevant matrices, such as human blood serum and plasma, frequently lead to compromised assays. Several surface chemistries have been de...
Article
Three-dimensional (3D) printing has undergone an exponential growth in popularity due to its revolutionary and near limitless manufacturing capabilities. Recent trends have seen this technology utilized across a variety of scientific disciplines, including the measurement sciences, but precise fabrication of optical components for high performance...
Article
The ability to monitor biomolecular reactions in a real-time and label free manner is nearly synonymous with the surface plasmon resonance (SPR) technique. At its basis, SPR relies on the interaction of an incoming light source with a thin metallic film in close contact with a prism or grating, which, under the correct experimental conditions, allo...
Article
Dual-functional cupric oxide nanorods (CuONRs) as peroxidase mimics are proposed for the development of a flow-through, label-free chemiluminescent (CL) immunosensor. Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simple hydrothermal method, were deposited onto epoxy-activated standard glass slides, fo...
Article
Self-folding deep cavitands with variably functionalized upper rims are able to selectively immobilize proteins at a biomimetic supported lipid bilayer surface. The immobilization process takes advantage of the dual-mode binding capabilities of the hosts, combining a defined binding pocket with upper rim charged/H-bonding groups. A variety of prote...
Article
A novel bioconjugation strategy leading to ultrastable gold nanoparticles (AuNPs), utilizing DNA linkers and diluents in place of traditional self-assembled monolayers, is reported. The protective capacity of DNA confers straightforward biomolecular attachment and multi-step derivatization capabilities to these nanoparticles, and more significantly...
Poster
Full-text available
Gold nanoparticles have been established as an ideal signal enhancement tool in a wide variety of sensing schemes and detection modalities. We report a novel conjugation strategy based on DNA linkers and diluents in place of conventional self-assembled monolayers. For model biotin-streptavidin systems, the nanoparticles were assembled through thiol...
Chapter
This chapter talks about nanohole arrays that are especially interesting for the combination of plasmonic techniques alongside electrochemical measurements, as they are simultaneously electrically conductive and support localized and propagating plasmons necessary for surface-enhanced techniques. It presents a brief introduction to the mechanisms o...
Article
Aminoglycoside antibiotics are used in the treatment of infections caused by Gram-negative bacteria, and are often dispensed only in severe cases due to their adverse side effects. Patients undergoing treatment with these antibiotics are therefore commonly subjected to therapeutic drug monitoring (TDM) to ensure a safe and effective personalised do...
Article
Therapeutic drug monitoring (TDM) is required for pharmaceutical drugs with dosage limitations or toxicity issues where patients undergoing treatment with these drugs require frequent monitoring. This allows for the concentration of such pharmaceutical drugs in a patient's biofluid to be closely monitored in order to assess the pharmacokinetics, wh...
Article
Full-text available
The ability to detect small molecules in a rapid and sensitive manner is of great importance in the field of clinical chemistry, and the advancement of novel biosensors is key to realising point-of-care analysis for essential targets. Testosterone is an example of such a small molecule, the detection of which is important in both clinical analysis,...
Article
The rapid, label-free and point-of-care detection of therapeutic drugs and other metabolites is of high importance for improving treatment of patients. SPR sensors were developed on a portable instrument for methotrexate, testosterone and antibiotic.
Article
Investigation into the use of artificial enzymes has become an increasingly popular area of research due to the numerous advantages offered in comparison to protein enzymes. One particular area of research interest involves the use of metal nanoparticles as artificial enzymes. The peroxidase-like activity of a variety of nanoparticles has recently...
Article
A novel method for analysing the catalytic action of a DNAzyme is reported. Resonance Raman scattering (RRS) is shown to successfully monitor the oxidation of two different peroxidase substrates and has been implemented in an assay for the detection of target DNA, providing a more sensitive method of analysis than current colorimetric techniques.
Article
The ability to develop new and sensitive methods of biomolecule detection is crucial to the advancement of pre-clinical disease diagnosis and effective patient specific treatment. Surface enhanced Raman scattering (SERS) is an optical spectroscopy amenable to this goal, as it is capable of extremely sensitive biomolecule detection and multiplexed a...
Article
SERRS (surface-enhanced resonance Raman scattering) is a vibrational technique, whereby a relatively weak Raman scattering effect is enhanced through the use of a visible chromophore and a roughened metal surface. The direct analysis of DNA by SERRS requires the modification of a nucleic acid sequence to incorporate a chromophore, and adsorption of...

Citations

... Human serum amyloid A1 (hSAA1), an acutephase reactant produced in inflammatory responses, has also arisen as a promising biomarker for lung cancer because the serum hSAA1 concentrations are higher in lung cancer patients (286 ng⋅mL −1 ; 24.44 nM) than normal subjects (34.1 ng⋅mL −1 ; 2.91 nM) [9,10]. It should also be noted that reports on the quantification of hSAA1 in undiluted clinical samples, which are challenging to detect because the target proteins are surrounded by a complex matrix, are scarce [11][12][13]. Therefore, the hSAA1 biomarker will be useful if evidence of target engagement in undiluted samples can be obtained in modest diagnostic tools to enable point-of-care testing, highlighting the validity of the diagnostic results. ...
... In a recent review, Lambert et al. [10] outlined the latest development on plasmonic surfaces manufactured using 3D printing as an emerging and challenging technology to produce complex devices. An interesting advancement was demonstrated by Hinman et al. [11] for SPR biosensing by 3D printing equilateral prisms via stereolithography using a commercial photoactive resin. However, to achieve the SPR required performance, polishing of the printed surfaces before gold sputtering was needed. ...
... Moreover, SPR can be produced in portable miniaturised forms, thus accentuating its utilisation by numerous research groups for different applications [88]. ...
... The catalytic activity of nanozymes can be regulated by hindering the binding of nanozyme and substrate. On the basis of this principle, Yang's team developed an efficient label-free chemiluminescence (CL) immunoassay based on the peroxidase activity of copper oxide nanorods, which was used to detect the broad-spectrum cancer marker in serum [46]. Biological screening of CuO nanoparticles showed that these nanoparticles exhibited promising antimicrobial activities against most of the resistant human pathogenic strains including both Gram-positive and Gramnegative bacteria [47]. ...
... Before proceeding with the functionalization, the MB were treated for 1 h at RT with a 100 times excess of tris(2-carboxyethyl) phosphine hydrochloride (TCEP) to remove the protecting groups of the thiol moieties [43]. Then, they were added to AuNP solution, vortexed, and shaken for 20 min for 15 nm AuNP or during 1 h for bigger particles (24 and 38 nm AuNPs). ...
... TDM of aminoglycosides has been the standard of care in many hospitals since the 1970s as a means to reduce toxicity while still assuring adequate levels of these antibiotics for treatment of severe infections. McKeating et al. showed how SERS can be used in combination with localized surface plasmon resonance (LSPR) for the analysis of various aminoglycosides and performed an in-depth study of the feasibility of carrying out TDM of tobramycin in spiked human serum, down to 4 µM [51]. The study was conducted using a 633 nm laser to excite gold nanoparticles conjugated with 5,5′dithiobis-(2-nitrobenzoic acid), DTNB. ...
... There is broad interest in utilizing biosensing technologies to help address global health challenges, especially in the context of developing improved diagnostic and therapeutic measures [1]. One of the most promising application areas involves drug development, in which case biosensors can offer mechanistic viewpoints on relevant interaction processes that complement and expand on biological testing [2,3]. Such capabilities have emerged as a promising approach to address the issue of antibiotic-resistant bacteria, which are a major threat to public health due to the overuse and misuse of antibiotics [4]. ...
... The limit of detection considered 0.05 ng/ml or 0.17 nm. 64 Another SPR-based biosensor for testosterone screening was developed using the molecular imprinting technique. Molecularly imprinted polymers (MIPs) through ultraviolet (UV) photopolymerization have been synthesized from methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) monomers with cavities for testosterone recognition. ...
... 18,19 Unlike previously reported research, the presented mechanism involves the catalytic activity of AuNSt for the oxidation of 3,3′,5,5′-tetramethybenzidine (TMB) in the presence of H 2 O 2 to produce oxidised TMB (oxTMB), a highly Raman active product. [20][21][22] We observed that in the presence of Hg 2+ ions, the Au-Hg amalgamation could even enhance the catalysed oxidation reaction as evident by the strong blue coloured oxTMB product. As stated, oxTMB is also a Raman-active residue, therefore one would reasonably expect a strong Raman signal owing to the SERS effect of AuNSts. ...
... Although the electrochemical method is effective for the detection of H2O2, the lack of selectivity for interference such as that by oxidative species, because of the similarity of their potential to that of H2O2, limits its practical application [22]. Compared with the electrochemical method, SERS exhibits remarkable advantages, such as its indestructibility, fingerprinting, ultrasensitivity, and selectivity [53][54][55][56][57]. Therefore, various NPs, such as Ag and Au nanostructures as SERS substrates, have been fabricated for detecting H2O2 [2,[50][51][52]. ...