Kristopher M Curtis's research while affiliated with United States Army Medical Research Institute for Infectious Diseases and other places

Publications (11)

Article
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The severe acute respiratory syndrome coronavirus (SARS-CoV) caused a worldwide epidemic in late 2002/early 2003 and a second outbreak in the winter of 2003/2004 by an independent animal-to-human transmission. The GD03 strain, which was isolated from an index patient of the second outbreak, was reported to resist neutralization by the human monoclo...
Article
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In 2003, severe acute respiratory syndrome coronavirus (SARS-CoV) was identified as the etiological agent of severe acute respiratory syndrome, a disease characterized by severe pneumonia that sometimes results in death. SARS-CoV is a zoonotic virus that crossed the species barrier, most likely originating from bats or from other species including...
Data
Titers and PRNT80 Dilutions for Individual Senescent Mice (146 KB DOC)
Article
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Molecular Therapy (2005) 11, S385|[ndash]|S385; doi: 10.1016/j.ymthe.2005.07.543 996. Development of an siRNA Based Therapy for Ebola Virus Infection Thomas W. Geisbert1, Lisa E. Hensley1, Kristopher M. Curtis1, Joan B. Geisbert1, Kathleen M. Daddario2, Elliott Kagan2, Amy C.H. Lee3,|[ast]|, Lorne Palmer3,|[ast]|, Lloyd Jeffs3,|[ast]| and Ian MacL...
Article
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Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcrip...
Article
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A previously undescribed coronavirus (CoV) is the etiologic agent responsible for severe acute respiratory syndrome (SARS). Using a panel of contiguous cDNAs that span the entire genome, we have assembled a full-length cDNA of the SARS-CoV Urbani strain, and have rescued molecularly cloned SARS viruses (infectious clone SARS-CoV) that contained the...
Article
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We have recently isolated a transmissible gastroenteritis virus (TGEV) infectious construct designated TGEV 1000 (B. Yount, K. M. Curtis, and R. S. Baric, J. Virol. 74:10600-10611, 2000). Using this construct, a recombinant TGEV was constructed that replaced open reading frame (ORF) 3A with a heterologous gene encoding green fluorescent protein (GF...
Article
The availability of infectious full-length cDNA clones is important for the molecular genetic analysis of the structure and function of RNA virus genomes (Ahlquist et al, 1984; Boursnell et al., 1987). Infectious cDNA clones for a number of positive-stranded RNA viruses have been developed, advancing our understanding of the molecular mechanisms of...
Article
Mouse hepatitis virus (MHV), a member of Nidovirales, contains a -32 KB linear, single-stranded, positive polarity RNA genome. Upon entry into the cell, the viral genome is transcribed into 7-8 subgenomic mRNAs ranging in size from ∼1.0-32.0 KB. The positive strand mRNAs are arranged in a 3′ co-terminal nested set, and each contains a 5’ end ∼72 nu...
Article
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A systematic method was developed to assemble functional full-length genomes of large RNA and DNA viruses. Coronaviruses contain the largest single-stranded positive-polarity RNA genome in nature. The approximately 30-kb genome, coupled with regions of genomic instability, has hindered the development of a full-length infectious cDNA construct. We...

Citations

... Until now, NAbs detection has been important in vaccine development and determining seroprevalence to assist the government in adjusting policy decisions. The plaque reduction neutralization test (PRNT) is considered the gold standard for measuring neutralization antibodies against SARS-CoV-2 [10][11][12]. Briefly, serial dilutions of the patient specimen (sera) are incubated with the target virus to form an immune complex. ...
... N-protein is a structural protein that also has a role in transcription and disease development. Nprotein expression is critical for successful viral recovery from cDNA clones that are infectious Yount et al., 2003), and it has recently been shown to increase HCoV-229E genome RNA replication (Schelle et al., 2005). Fulminant hepatitis has been connected to the MHV N-protein. ...
... The design of a multi-epitope vaccine depends on the identification and assembly of B-and T-cell epitopes that are capable of stimulating the humoral and cell-mediated immune. [7][8][9][10][11][12][13][14] In this the current study, using immunoinformatics tools [11,[15][16][17][18][19][20][21] we predicted the cytotoxic T-lymphocyte (CTL), helper T-lymphocyte (HTL), and B-cell epitopes of spike protein from an isolate of our study and analyzed the conservancy and other immunological properties with respect to various Indian and global strains representing all clades including the new variant SARS-CoV-2 through immunoinformatic tools. We also investigated the population coverage of B-and T-cell epitopes from various countries affected by COVID-19. ...
... An RGS for coronavirus, however, is challenging due to instability of its large viral genome in Escherichia coli (E. coli), with previous studies using in vitro ligation of viral cDNA fragments and recombination in a vaccinia vector to construct a full-length viral genome Thiel et al., 2001;Yount et al., 2000). Bacterial artificial chromosomes (BACs) are well-established for stably maintaining large cloned DNAs as a single copy in E. coli, and a BAC-based RGS has been used for the genomic manipulation of herpesvirus, which has a genome larger than 120 kb (Messerle et al., 1997;Shizuya et al., 1992), and various coronavirus species (Almazán et al., 2006;Almazán et al., 2013Almazán et al., , 2000Balint et al., 2012;Lv et al., 2020). ...
... Infectivity and replication of such a recombinant MHV are poor, indicating that, while Eis not required for MHV, it is vital in the creation of infectious viruses (Kuo et al., 2003). However, the E-protein of the transmissible gastroenteritis virus (TGEV) is required; interruption of Eprotein gene within TGEV proteins is fatal (Curtis et al., 2002;Ortego et al., 2002). In plays important role in viral replication, E-protein performs a number of other functions during infection. ...
... It turned out that the recombinant plasmids were not stable, generating different mutations in the viral genome during the screening of the infectious cDNA clone. We then explored in vitro ligation strategy [14][15][16][17] for clone construction. Using the extracted viral RNA as a template, four cDNA fragments (F1, F2, F3 and F4) covering the complete genome were amplified through a reverse transcriptase-PCR (RT-PCR) with four pairs of primers (Table S1, available in the online Supplementary Material) containing different BglI restriction sites. ...
... www.rnajournal.org (1995), which proposes a discontinuous step during minusstrand RNA synthesis, is best supported by the available data from both biochemical and genetic studies with arteriviruses and coronaviruses (Sethna et al. 1989;Schaad et al. 1990;den Boon et al. 1996;Baric et al. 2001;Pasternak et al. 2001;Sawicki et al. 2001). According to this model (Fig. 1B), minus-strand RNA synthesis would be attenuated when the RdRp complex encounters a body TRS in the plus-strand genomic template. ...
... SNP_251 (LG_1) is the only SNP located in the noncoding region. It is located at the leader sequence (Sawicki et al. 2007) in front of ORF1 and may affect the protein-to-RNA interaction (Pasternak et al. 2004(Pasternak et al. , 2006Sawicki et al. 2007) in the assembly into membrane-bound replication-transcription complexes (Curtis et al. 2004;Z uñiga et al. 2004;Sola et al. 2005;Enjuanes et al. 2006;Yount et al. 2006). Three SNPs are located in ORF1ab. ...
... The S protein presents on the viral membrane and mediates the virus attachment with the host cells (9).Hence, the S protein is considered a key target for SARS-CoV-2 vaccine development, as it can induce neutralizing antibodies that prevent viral uptake through the human ACE2 receptor (10,11). Previous research on SARS-CoV had shown that the protection from infection in mouse models was attributed to antibody responses generated against the S protein (12)(13)(14). In addition, multiple studies suggested that the N protein of SARS-CoV, abundantly expressed protein during infection, appears to be highly immunogenic (15). ...