Krishna Pundi’s scientific contributions

Objective: The present research study was conducted to investigate in vitro anti-diabetic potentials of ethanol extract of leaves of Muntingia calabura for α-glucosidase and α-amylase inhibitory activities. Materials & Methods: The leaves of Muntingia calabura were collected and authenticated. dried and powdered. The powdered drug was defatted using petroleum ether and extracted with ethanol. The ethanol extract of Muntingia calabura was subjected to preliminary phytochemical investigation. The in vitro α-glucosidase inhibitory and α-amylase inhibitory properties were determined for the ethanol extract and concentrations of extract required to inhibit absorbance (IC50 values) EEMC were determined. Results: Significant IC50 values were found in both tests that assessed the inhibitory activity of α-glucosidase and α-amylase, demonstrating the capacity of the ethanol extract of Muntingia calabura to block carbohydrate digesting enzymes and prevent postprandial blood glucose. Conclusion: The results of the presents investigation recommends that ethanol extract of Muntingia calabura possess significant α-glucosidase and α-amylase inhibitory activity. Keywords: Antidiabetic activity, Muntingia calabura, α-glucosidase and α-amylase and IC50values.

The present investigation was designed to evaluate in vitro cytotoxic potentials of ethanol extract of Syzygium cumini against breast cancer cells and also estimate its antioxidant activity. The ethanol extract of Syzygium cumini was prepared by successive solvent extraction process using sox-let apparatus. The ethanol extract was evaluated for its in-vitro anticancer potentials against MCF-7 human breast cancer cell lines by MTT assay and concentration of the ethanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, superoxide scavenging, lipid peroxidation and DPPH methods were used and IC50 values of extracts were noted. In current study, ethanol extract of Syzygium cumini has shown anticancer property by exhibiting significant IC50 values against growth of breast cancer cell growth. The extract has also shown significant IC50 values in superoxide scavenging, lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid. The results obtained from the present research work suggested that the ethanol extract of Syzygium cumini possess significant in vitro cytotoxic potentials against breast cancer cells. The results also suggested that the ethanol extract have in vitro antioxidant properties. Keywords: Anticancer activity, Syzygium cumini, IC50 value, MTT assay and antioxidant activity.