Koichi Noguchi's research while affiliated with Hyogo College of Medicine and other places

Publications (48)

Article
Full-text available
We studied the effects of OT-7100 (5-n-butyl-7-(3,4,5-trimethoxybenzoylamino)pyrazolo [1,5-a]pyrimidine), a novel analgesic compound, on the inhibitory action of adenosine on the contraction of guinea pig ileum and investigated the effects of OT-7100 on the nociceptive responses in animal models of inflammatory and peripheral neuropathic hyperalges...
Article
Full-text available
We investigated the effects of OT-7100, a novel analgesic compound (5-n-butyl-7-(3,4,5-trimethoxybenzoylamino)pyrazolo[1,5-a]pyrimidi ne), on prostaglandin E2 biosynthesis in vitro, acute hyperalgesia induced by yeast and substance P in rats and hyperalgesia in rats with a chronic constriction injury to the sciatic nerve (Bennett model), which is a...
Article
We examined the colocalization of trk family and low-affinity neurotrophin receptor (LANR) mRNAs by means of in situ hybridization histochemistry in rat dorsal root ganglion (DRG) neurons. About 35-40%, 5%, and 15-20% of DRG neurons were positive for trkA, trkB, and trkC mRNAs, respectively. Each member of the trk family was coexpressed with LANR....
Article
It has been suggested that myogenin is an important factor for the differentiation of myoblasts and that its function in myogenesis is regulated by proto-oncogenes in in vitro experiments. We have characterized the spatial and temporal expression patterns of myogenin, c-fos, c-jun, and muscle creatine kinase mRNAs during the skeletal muscle regener...
Article
We aimed to clarify the regulatory mechanism of the hypothalamo-pituitary-adrenal axis that plays key roles in initiating stress responses, as well as the roles of immediate early genes in this process. We investigated the stress-induced activation of fos and jun family proto-oncogenes by means of in situ hybridization histochemistry. Immobilizatio...
Article
By in situ hybridization and immunohistochemistry, we examined the expression of neuropeptides such as neuropeptide Y (NPY), galanin (Gal), substance P (SP), vasoactive intestinal polypeptide (VIP) and their mRNAs in the rat mesencephalic trigeminal nucleus (Mes5) following masseteric nerve transection. On the side contralateral to the nerve transe...
Article
Repeated stress is known to potentiate the CNS response to subsequent stress. Various stressful stimuli can induce Fos expression in discrete regions of the brain, such as the lateral septum, the hypothalamic paraventricular nucleus and the locus coeruleus. We investigated by immunohistochemistry the effect of the stress of repeated immobilization...
Article
Neuropeptide Y (NPY)-like immunoreactivity (IR) was observed in 20-30% of ipsilateral dorsal root ganglion (DRG) neurons (L4-5) after unilateral transection of rat sciatic nerve. Most of these neurons contained 200 kDa subunit of neurofilaments and galanin. Immunohistochemical analysis combined with retrograde tracing method demonstrated that NPY-I...
Article
The purpose of the present study was to determine changes in the localization of vinculin, Type IV collagen, laminin, and fibronectin during the damage-repair process in the skeletal muscle over several days following acute blunt trauma. A single blunt trauma to the gastrocnemius muscle of the rat was induced by applying 1.57 J of kinetic energy to...
Article
To clarify which brain regions are activated by stress, we used expression of the proto-oncogene, c-fos, as a marker. An increased number of neurons expressing Fos-like immunoreactivity in their nuclei was observed in discrete brain regions, such as the lateral septum, midline nuclei of the thalamus, paraventricular hypothalamic nucleus, brain stem...
Article
Axonal flow of motoneurons was blocked in the sciatic nerve by the application of vinblastine at a low concentration (100 microM) not considered likely to cause axonal degeneration. The number of motoneurons expressing alpha-calcitonin gene-related peptide (CGRP) mRNA was significantly increased by vinblastine application, while the expression of b...
Article
In situ hybridization histochemistry was used to study changes in mRNAs coding neuropeptides such as alpha-calcitonin gene-related peptide (CGRP), beta-CGRP, cholecystokinin (CCK) and galanin, in rat facial motoneurons following axotomy of the facial nerve. In control rats, 38%, 55% and 7% of the facial motoneurons expressed alpha-CGRP, beta-CGRP a...
Article
Gamma-aminobutyric acid (GABA) is a well known major inhibitory neurotransmitter in the vertebrate brain. Its inhibitory action is mediated through the activation of the specific receptors: the GABAA and GABAB receptors. Binding of GABA with GABAA receptor (GABAA-R) causes the chloride channel to open (for reviews see Roberts, 1986; Stephenson, 198...
Article
We found that the level of nerve growth factor receptor (NGF-R) mRNA in facial motoneurons was increased after both facial nerve crushing and transection by means of in situ hybridization histochemistry. The increased level of NGF-R mRNA was maintained for at least 8 weeks after facial nerve transection, while facial nerve crushing caused only a tr...
Article
The expression of mRNAs encoding different beta subunits (beta 1, beta 2 and beta 3) of GABAA receptor in the rat olfactory bulb and some of its related areas was investigated by in situ hybridization histochemistry. The beta 3 subunit mRNA was expressed in most of the cells in these regions such as periglomerular cells, tufted cells, mitral cells...
Article
The ontogeny of the expression of prepro-neurotensin/neuromedin N messenger RNA (prepro-NT/NN mRNA) in the rat posterior cingulate cortex (retrosplenial cortex) and the hippocampal formation was investigated using in situ hybridization histochemistry. In the primordium of the posterior cingulate cortex and the hippocampal formation, prepro-NT/NN mR...
Article
We found an increase in calcitonin gene-related peptide (CGRP)-like immunoreactivity in motoneurons of rat spinal cord after peripheral axotomy. By means of in situ hybridization histochemistry and Northern blotting, we further demonstrated that this increase was the result of increased levels of α-CGRP mRNA, not β-CGRP mRNA. The increased level of...
Article
Postnatal developmental changes of preproenkephalin (PPE) gene expression in rat brainstem neurons were studied by in situ hybridization histochemistry. On the basis of PPE mRNA expression, brainstem neurons were categorized into three types: 1 type I neurons were characterized by constant or increasing expression of PPE mRNA during postnatal devel...
Article
Using in situ hybridization histochemistry, we examined the expression of prepro-vasoactive intestinal polypeptide (VIP) mRNAs and preprotachykinin (PPT) mRNAs which coded for substance P (SP) in the rat dorsal root ganglion (DRG) following spinal nerve transection. VIP mRNAs increased dramatically in the DRG neurons after transection of the periph...
Article
We used in situ hybridization histochemistry to examine the postnatal development of the somatostatin (SRIF) synthesizing system in the cerebellum of rats. There are numerous hybridizing neurons from 1 to 9 days after birth. These occur throughout the cerebellum including the developing medulla and cortex except in the external granular cell layer....
Article
We examined the expression of α-calcitonin gene-related peptide (α-CGRP) and β-CGRP mRNAs in the rat dorsal root ganglion (DRG) using in situ hybridization histochemistry with oligonucleotide probes. Analysis of autoradiograms showed that α-CGRP mRNA labeled 36.4% of all DRG neurons and β-CGRP mRNA labeled 30.8%. Small and medium-sized neurons expr...
Article
The trans-synaptically activated biosynthesis of the preproenkephalin (PPE) mRNA in the dorsal horn of the rat lumbar spinal cord was examined by an in situ hybridization histochemical technique. As a nociceptive stimulus, a small amount of formalin was injected into the right hindpaw, and quantitative and qualitative changes of PPE-mRNA expression...
Article
Postnatal development of proopiomelanocortin gene expression in the rat pituitary was examined using in situ hybridization histochemistry. In adult rats, a very high density of hybridization signals was seen in the intermediate lobe of the pituitary, while only a moderate density occurred in the anterior lobe. No evidence of hybridization was detec...
Article
Full-text available
We reviewed 36 cases of forearm deformity caused by multiple osteochondromas in 30 patients and classified them into three types: Type I showed a combination of ulnar shortening and bowing of the radius secondary to osteochondromas of the distal ulna (22 forearms). Type II showed dislocation of the radial head, either with osteochondromas of the pr...
Article
Using in situ hybridization histochemistry, we examined the biosynthesis of the preprotachykinin-A (PPTA) mRNAs, which code for substance P (SP) and also a related peptide, substance K (SK), in the rat dorsal root ganglion (DRG). The animals were injected with a small amount of formalin in the right hindpaw for nociceptive chemical stimulation. Ana...

Citations

... Regardless of origin, CCK receptors may have specific functional links to neuronal development and survival (reviewed in Tirassa et al. 2002). This is suggested by their ability to undergo developmental changes ( Cho et al. 1983), to modulate neurite outgrowth and by their neurotrophic action in cultured ventral spinal cord neurons ( Iwasaki et al. 1989a,b), and to participate in recovery after axotomy in adults (Palkovits 1995;Saika et al. 1991;Schiffmann et al. 1991). It is also possible that their influence on excitability of neonatal spinal cord neurons is important for their subsequent development (see following text). ...
... The impact of LC hyperactivity and degeneration likely extends beyond alterations in noradrenergic function. Besides NE, LC neurons produce a host of other small molecule and peptide neuromodulators including ATP [114], galanin [117,118], neuropeptide Y [117], enkephalin [119], cocaine-and amphetamine-regulated transcript [120], and brain-derived neurotrophic factor (BDNF) [121,122]. Galanin and BDNF are of particular interest because they have neurotrophic properties and have been directly implicated in neurodegenerative disease [123][124][125][126]. Thus, although LC neurons are generally classified as 'noradrenergic', the disruption of multiple neuromodulator signaling pathways may underlie the consequences of altered LC activity and function in AD and PD. ...
... This is consistent with migraine patients, where elevated plasma CGRP-LI has been observed during acute migraine attacks and in chronic migraine patients compared with healthy controls (Goadsby et al., 1990;Irimia et al., 2020). Interestingly, this elevation has been reported for up to 3 days following an acute migraine attack, suggesting sustained CGRP transcription and synthesis and/or release (Durham, 2006 Bhatt et al., 2014;Edvinsson et al., 1998;Gibson et al., 1984;Lopes et al., 2017;Manteniotis et al., 2013;Ray et al., 2018;Tajti et al., 1999) β (Costigan et al., 2002;Flegel et al., 2015;Manteniotis et al., 2013;Noguchi et al., 1990aNoguchi et al., , 1990bRay et al., 2018;Toribio et al., 2003) Amylin Costigan et al., 2002;Ferrier et al., 1989;Flegel et al., 2015;Isensee et al., 2014;Kogelman et al., 2017;Manteniotis et al., 2013;Martinez-Alvarez et al., 2008;Mulder et al., 1995Mulder et al., , 1997aMulder et al., , 1997bNicholl et al., 1992;Ray et al., 2018) Chen et al., 2016;D. Wang, Huo, et al., 2014;D. ...
... 99 The PNS interrupts synapses and switches to a regenerative state, 100-102 reducing the production of neuropeptides for regeneration-associated metabolism, as shown by the downregulation of synthesis in the perikarya. [103][104][105][106] However, neuropeptides, such as CGRP and SP, are thought to be increased in the fracture region. 107,108 Apart from the fact that activation of the PNS directly contributes to the release of neurotransmitters, 109 synthesis in injured axons (axonal synthesis) rather than the perikarya during regeneration partly accounts for the increase in neurotransmitters. ...
... After peripheral nerve injury (PNI), primary sensory neurons with cell bodies in dorsal root ganglia (DRGs) undergo functional alterations leading to amplification of noxious and innocuous stimuli (hyperalgesia and allodynia) and continuing activation of central pain pathways 1,2 , in part via increased electrical activity in primary somatosensory neurons 1, [3][4][5] . Alterations in gene and protein expression in DRG neurons have been linked to neuropathic pain [6][7][8][9] . PNI can produce axonal injury, degeneration, regeneration, and sprouting, plus synaptic remodeling, inflammatory responses, death of DRG cells, and altered electrical activity of sensory neurons [10][11][12] . ...
... The effects of axonal transport disruption along intact axons can be examined by applying low doses of the vinca alkaloid vinblastine to the rat sciatic nerve (Dilley and Bove 2008a;Fitzgerald et al. 1984). At low doses, perineural vinblastine disrupts microtubule polymeriza-tion without causing axonal degeneration or inflammation (Dilley and Bove 2008a;Fitzgerald et al. 1984;Kashiba et al. 1992;Katoh et al. 1992;Zhuo et al. 1995), which allows axonal transport disruption to be examined in the absence of these potentially confounding factors. Using this model, we have shown that localized vinblastine treatment causes the development of a transient tactile-evoked cutaneous hypersensitivity . ...
... GABA is the major inhibitory neurotransmitter in the adult brain and acts primarily by binding to GABA A or GABA B receptors, but in early development GABA actions can be excitatory and may function as a trophic signal (Barbin et al., 1993;Behar et al., 1996;Ben-Ari et al., 1988;LoTurco et al., 1995). Changes in GABAergic function during development arise in part from alterations in the chloride ion reversal potential (Owens et al., 1996;Zhang et al., 1991) mediated by changes in expression of chloride transport proteins (Rivera et al., 1999). The GABA A receptor gates a Cl − channel, and in the mature brain, GABA activation causes Cl − influx and membrane hyperpolarization due to the low intracellular Cl − concentration established by the K + -Cl − co-transporter KCC2 (Li et al., 2002;Rivera et al., 1999). ...
... In rats with nerve transection injury, this difference lasted more than 8 weeks, whereas those with a crush injury showed a temporary change. Subsequent measurements showed normal expression levels during nerve regeneration [45,46]. ...
... Although statistically derived factors identified through PCA studies are difficult to interpret due to their multivariate nature and the number of samples in this preliminary study was limited, the large explanation of variance by PCA factors in controlling regenerating muscles suggests new possibilities for the role of P75-expressing cells. Schwann cells are known to respond to axonal injury by transiently expressing several developmental genes (Lemke and Chao, 1988;Saika et al., 1991;Lee et al., 2009). P75 upregulation by mature sensory neurons after axonal injury (Hassan et al., 1994) or distal nerve stumps after nerve transection (Toma et al., 1992) was proposed as a time-dependent indicator of the injury response by TSCs and Schwann cells (Roberson et al., 1995). ...
... Receptor type Subunit mRNA Protein GABA A Ligand-gated Cl − channel alpha1 +++ (Duncan et al., 1995) +++ ICj a , + L1-3 (Pirker et al., 2000;Schwarzer et al., 2001) alpha2 +++ L1-3 (Pirker et al., 2000;Schwarzer et al., 2001) alpha3 + (Schwarzer et al., 2001) alpha4 +++ L1-3 (Pirker et al., 2000;Schwarzer et al., 2001) alpha5 + (Schwarzer et al., 2001) alpha6 beta1 − (Zhang, Sato, Noguchi, & Tohyama, 1990) + (Schwarzer et al., 2001) beta2 +++ (Duncan et al., 1995), + (Zhang et al., 1990) ++ ICj a , + L1-3 (Pirker et al., 2000;Schwarzer et al., 2001) beta3 +++ L2-3 (Zhang et al., 1990) +++ L1-3 (Pirker et al., 2000;Schwarzer et al., 2001) gamma1 − (Schwarzer et al., 2001) gamma2 ++ (Duncan et al., 1995) + (Schwarzer et al., 2001) gamma3 − (Schwarzer et al., 2001) delta ++ L1-3 (Schwarzer et al., 2001) GABA B GPCR 1a +++ (Charles et al., 2001) 1b +++ (Charles et al., 2001) 2 + (Durkin et al., 1999) +++ (Charles et al., 2001) Note: The absence of data reflects no literature available/found, or region not specified. All data cited is from non-human animal models. ...