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... Step 1. Identification of Reference and Degraded sites (sampling stations): Reference and Degraded sites were identified and designated based on selection criteria ( Table 1) (Stribling et al., 1998) (Stribling et al., 1998) (Stribling et al., 1998) (Chauhan et al., 2011) Step 2. Compiling and calculating candidate metrics: Candidate metrics for testing and potential inclusion in the M-IBI were nominated primarily by assessing the previous work done, parallel studies or documents/ protocols/ guidelines published by varies authors or institutes such as Stribling (1998), Chirhart (2003, Macroinvertebrates as bio-indicators (2008), Jensen (2007), Calculating and Interpreting the Genus-Level B-IBI (2001), Bennett and Rysavy (2003), Wittman and Mundahl (2002), Weigel et al. (2002), Mandaville (2002) and Karr et al. (2002). These Macroinvertebrate based 41 candidate metrics were grouped in to five and listed out with their possible response to environmental stresses (stream impairment) in Table 2. ...