December 2023
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5 Reads
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December 2023
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5 Reads
September 2022
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9 Reads
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3 Citations
Environmental Biology Research
January 2022
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48 Reads
A marine green algal species was collected from the eastern coast of Korea. This species shares the generic features of Ulva , and is characterized by irregularly shaped thalli, relatively small and thick thallus, entire undulate margins without serrations and one or two pyrenoids per cell. In a phylogenetic tree based on sequences of the nuclear-encoded internal transcribed spacer region (ITS) of ribosomal (r)DNA, it nests as a sister clade to some species including U. ohnoi with a relatively large thallus. This Korean alga differs from those species forming the same or subclades, such as U. ohnoi , U. fasciata, U. reticulata , and U. gigantean , in having a relatively small (3–8 cm) and thick (60–100 µm) thallus. Of these species, U. ohnoi originally described from Japan is similar to the Korean alga in having a more or less thick thallus of 30–90 µm, but is distinguished from the Korean species in often having microscopic serrations in the thallus margin. The genetic distance between this Korean entity and those species was calculated as 1.8–4.8%, which is considered to be an inter-specific divergence level with the genus Ulva . Ulva grossa sp. nov. (Ulvales, Chlorophyta) is described from Korea based on the morphological and molecular analyses herein.
December 2020
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13 Reads
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2 Citations
Environmental Biology Research
September 2020
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20 Reads
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3 Citations
Environmental Biology Research
September 2020
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8 Reads
Environmental Biology Research
November 2019
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281 Reads
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1 Citation
A green alga was collected from Daejin, located on the eastern coast of Korea. It shows a typical siphonous thallus structure composed of erect and prostrate filaments like other Derbesia sporophytes and is characterized by a relatively large sporophytic thallus (6–8 cm) without calcified attachment disk, irregularly or subdichotomously branched filaments with a basal septum and 1(2) pyrenoid per ovoid to lenticular, occasionally irregular chloroplast. Sporangia have an obovoid or pyriform shape with a short pedicel and septum at the base. Vesicular gametophytes are spherical or ovoid, with short anchoring stalk and rhizoids. A heteromorphic life history alternating between sporophytic filaments and gametophytic Halicystis stage was observed. In a phylogenetic tree based on rbcL sequence, the Korean entity nests in a sister clade to several species groups including the type Derbesia marina, and of these species, D. fastigiata is closest to the Korean alga with a genetic divergence of 3.1%. However, it is distinguished from the Korean species by the size of sporophytic thalli, branching pattern, basal constriction of laterals, and shape of sporangia. The other species forming the sister clade are also distinguished from the Korean entity by general morphological features of sporophytes and gametophytes. The specific distinction of the Korean alga from those species is supported by its genetic divergence, which is considered to be involved in the inter-specific range for the genus based on the previous data. Accordingly, Derbesia maxima sp. nov. is proposed for this siphonous green alga from Korea.
September 2019
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15 Reads
Environmental Biology Research
September 2019
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13 Reads
Environmental Biology Research
June 2019
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2,241 Reads
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24 Citations
To examine optimal growth conditions of the edible green alga Caulerpa okamurae, thalli of this species were cut into erect frond (3 cm long) and stolon (5 cm long) fragments. They were cultured in combinations of four temperatures (15, 20, 25, and 30 °C), three irradiances (20, 40, and 80 μmol photons m⁻² s⁻¹), four daylengths (8, 12, 16, and 24 h), and seven salinities (15, 20, 25, 30, 35, 40, and 45 psu). After 12 days, maximal relative growth rates (RGRs) for erect fronds (6.78% day⁻¹) and stolons (7.02% day⁻¹) occurred at 25 °C and 40 μmol photons m⁻² s⁻¹. At 30 °C, growth differences were found; negative growth for erect fronds and positive growth for stolons, with an average RGR of 1.96% day⁻¹. A daylength of 16 h and salinity of 30 psu were needed for maximal growth of erect fronds and stolons. Stolon exhibited a greater tolerance to high and low temperatures and low salinity than erect fronds. Thalli weight of C. okamurae was increased by increments of stolon length, new erect fronds, and new ramuli. In conclusion, C. okamurae should be cultivated in summer with high seawater temperature (25 °C) and long daylength, in waters of 30 psu, and at a water depth providing 40 μmol photons m⁻² s⁻¹. These physiological and growth pattern data are informative for the successful development of commercial cultivation of C. okamurae.
... Both genera have a central vegetative axis with two pericentral cells, spermatangial structures with the filament type (usually in the apical urn-like depression), and tetrasporangia are formed from random epidermal cells. These features distinguish these genera from representatives of Eujanczewskia, which are anatomically close to Laurencia [51,52]. ...
September 2022
Environmental Biology Research
... Total DNA was extracted from fresh or dried samples using the Chelex method (Zuccarello et al. 1999). The partial sequence of the plastid-encoded ribulose-1, 5-bisphophate carboxylase/oxygenase large subunit gene (rbcL) was amplified using the primer pairs F321 and R1150 (Kang et al. 2020). PCR conditions followed a touchdown PCR as follows: initial denaturation at 94°C for 4 min, followed by 10 cycles of 94°C for 1 min, 55°C for 30sec, and a decrease in annealing temperature by 1°C per cycle, and 72°C for 1 min; followed by 25 cycles of 94°C 1min/45°C, 30sec /72°C for 1 min and a final step at 72°C for 5 min. ...
December 2020
Environmental Biology Research
... Our study demonstrates how DNA sequences can clarify the identity of misidentified samples. Kang et al. (2020) reported C. retortus in Honghyeon-ri, Namhae, Korea based on the rbcL gene. It is possible that this species has been established in other localities around the Pacific region, but historically misidentified and labeled as C. ocellatus. ...
September 2020
Environmental Biology Research
... This can result in better growth and potentially more ramuli. In the study Gao et al. (2019) new ramuli could be found on both new erect talus and fragments of early erect talus. The number of new ramuli was significantly greater at 25°C than at 15 and 20°C. ...
June 2019
... Even with the recent investments in molecular data and greater sampling intensity (Geraldino et al., 2009(Geraldino et al., , 2010Jesus et al., 2016Jesus et al., , 2019aNauer et al., 2014Nauer et al., , 2015Nauer et al., , 2016Nauer et al., , 2019Kang and Nam, 2018;Cabrera et al., 2020;Huisman et al., 2021;Kundu and Bast, 2021), Hypnea remains a source of several taxonomic problems, with challenging species delimitation (Price et al., 1992;Jesus et al., 2016Jesus et al., , 2019a. This has been pronounced not only in the most species-rich genus of Cystocloniaceae, but also in the family as a whole, as molecular efforts have been limited to a few genes so far (Hommersand and Fredericq, 2003;Won et al., 2016;Yang and Kim, 2017;Hoffman et al., 2018;Soares and Fujii, 2020;Tonicelli et al., 2021). ...
December 2018
Fisheries and Aquatic Sciences
... Crouan et H. Crouan) Batters, is a filamentous alga composed of cylindrical cells containing parietal chloroplasts with multiple pyrenoids and twisted setae (Batters 1902;Correa et al. 1988;Kitayama & Yoshida 1992). This alga has also been reported as an epiphyte or endophyte of seaweed such as brown and red algae (Srinivasan 1961;Kitayama & Yoshida 1992;Kim et al. 2017). Given its existence within coral skeletons and its ability to share habitats with Ostreobium sp., we hypothesized that it may be able to acclimate to FRL in this severe environment. ...
February 2017
Journal of Fisheries and Marine Sciences Education
... Similar zygote release and thallus recruitment have been observed in S. siliquosa studies in nearby areas, such as Geumgap in Jindo, Sinan, Heuksan, and Wando [21]. Additionally, the appearance rate of S. siliquosa thalli was higher in substrates where drying stress due to tidal exposure was reduced [36]. Therefore, securing attachment space, and the density and exposure conditions of existing thalli, likely played a decisive role in S. siliquosa recruitment. ...
December 2017
ALGAE
... and chloroplast (rbcL and tufA) barcoding markers (Figure 1). This is reflected in numerous records (Chávez-Sánchez et al. 2019;Hofmann et al. 2010;Kirkendale et al. 2013;Manghisi et al. 2011;Miladi et al. 2018;Steinhagen et al. 2019;Woo and Ki 2017;Xie et al. 2020) that have led to many rearrangements and taxonomic revisions of the Ulva species (Cui et al. 2018;Hughey et al. 2019Hughey et al. , 2021Hughey et al. , 2022Krupnik et al. 2018;Rybak et al. 2014). As recorded by Guiry and Guiry (2023), only 155 of the 622 taxa described at the species and infraspecific levels are currently accepted; additionally, 105 names are currently of uncertain status. ...
September 2017
Environmental Biology Research
... On July 24, the green tide's coverage area decreased to 25.0 km 2 (Fig. 2F) and by August 8 the main body had basically dissipated. Previous studies have shown that the optimum temperature for the growth of U. prolifera is 20-25°C (Nam, 2016;Wang et al., 2018;He et al., 2019), and the sea surface temperature in the Yellow Sea in late May and early mid-June 2022, was precisely between 20°C and 26°C (Fig. 3A). The green tide in the southern Yellow Sea blooms from April to August, so the nutrient concentration in the sea area in spring is very important. ...
December 2016
Korean Journal of Fisheries and Aquatic Sciences
... If this compound originated from endemic microflora, one might expect it to be present in the outer tissues of the bark rather than from the inner stipe tissues. Most research has focused on surface microflora in seaweeds (Singh and Reddy 2014) but specific endophytic microflora (e.g., Streblonema spp., Ectocarpus sp., Laminariocolax tomentosoides; Bolbocoleon spp., Acrochaete spp., Enteromorpha spp and Mikrosyphar zosterae) are known to invade the intercellular spaces of tissues of L. hyperborea, and also other seaweeds (e.g., Chondrus ocellatus; Ellertsdóttir and Peters 1997;Ogandaga et al. 2017). It may be relevant that many of these putative microbial metabolites were more abundant in winter samples (Fig. 4), which as beach-cast material with apparent damage due to storm events, may have allowed microflora invasion. ...
October 2017