Keyong Jiang's research while affiliated with Qingdao National Laboratory for Marine Science and Technology (QNLM) and other places

Publications (56)

Article
We cultured post-larval shrimp in four salinity treatment groups (SAL20 (control) = 20 ppt (parts per thousand), SAL10 = 10 ppt, SAL30 = 30 ppt, and SAL40 = 40 ppt) for 20 days (restriction stage) and then transferred them to the control salinity environment (20 ppt) for 20 days (compensation stage). The SAL40 group exhibited an over-compensation g...
Article
The redclaw crayfish (Cherax quadricarinatus) is being farmed on an increasing scale in various tropical and subtropical regions. The most distinctive feature of the adult male redclaw crayfish is the red cuticle patch on the lateral cuticle of the chelae. It is an important indicator of the health status, reproduction, and resistance to predators...
Article
Full-text available
Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus causing AHPND (VPAHPND) is the most serious disease affecting shrimp farming. The PirAvp and PirBvp toxins of VPAHPND are known virulence factors. However, the corresponding target protein in shrimp that mediates their action has not been identified. By screening yeas...
Article
In this study, limited water exchange was used to simulate small earthen ponds equipped with greenhouses for Penaeus vannamei culture by laying sediment in tanks, and we investigated the effects of treatment with potassium monopersulfate compound (KMPC) in multiple low‐dose (5 g/m3, three times every 2 weeks) and single high‐dose (15 g/m3, once eve...
Article
Shrimp nursery rearing technology is increasingly being used by aquatic farmers and enterprises. Determining an appropriate density restriction in order to obtain a greater compensatory growth potential is the key to this technology. Based on the industry standards for indoor shrimp farming, three densities for Litopanaeus vannamei nursery rearing...
Article
Kazal-type serine proteinase inhibitors (KPIs) function in physiological and immunological processes requiring proteinase action. In the present study, the first Cherax quadricarinatus KPI gene (designated CqKPI) was identified and characterized. The open reading frame of CqKPI contains 405 nucleotides and encodes a protein of 134 amino acids. CqKP...
Article
Full-text available
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that constitute a broad layer of gene regulation at both transcriptional and post-transcriptional levels from prokaryotes to eukaryotes. In embryonic development, they regulate the complex gene expression associated with the complexity of embryogenesis. There is little information about miRNAs...
Article
Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about them early. In this study, we describe a novel, sensitive and specific rapid isothermal recombinase polymerase amplification assa...
Article
Full-text available
The red claw crayfish (Cherax quadricarinatus) is an emerging and important commercial species in several countries, and is also a potential biological model in crustacean biology. However, its molecular embryonic development mechanism remains largely unknown because of a lack of genomic resources and systematic research. A comprehensive and integr...
Article
Full-text available
The Pacific white shrimp (Litopenaeus vannamei), as one of the most valuable crustaceans, is widely distributed in China. Dietary astaxanthin has multiple functions in L. vannamei, such as promoting growth and antioxidant production. In the current study, shrimps were fed a diet which was supplemented with 0, 50, or 100 ppm astaxanthin for 4 weeks,...
Article
Lactobacillus pentosus HC-2 is a lactobacillus previous isolated and identified from aquatic animals (such as the goby, Acanthogobius hasta), which has shown a positive effect on growth and disease resistance in the Pacific white shrimp, Penaeus vannamei. Aflatoxin B1 (AFB1) is a highly carcinogenic and teratogenic toxin found in moldy feeds. This...
Article
Raptor, a member of the target of rapamycin complex 1 (TORC1), participates in the formation of complex proteins related to the mechanistic target of rapamycin (mTOR) signalling. In this study, a 5,020 bp cDNA of Raptor with an open reading frame (ORF) of 3,804 bp encoding for 1,267 amino acids was cloned from Litopenaeus vannamei. The protein cont...
Article
Intestinal bacteria and bacterial metabolic products are indispensable components of both invertebrate and vertebrate physiology, directly influencing many functions including host energy absorption and metabolism, intestinal barrier integrity and immune function. To investigate the influence of rearing density on shrimp intestinal health, antioxid...
Article
Our previous work showed that using Lactobacillus pentosus HC-2 as a probiotic could improve the growth performance, immune response, gut bacterial diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism had not been fully characterized. In the present study, histology and proteomic analysis were performed to expl...
Article
The present study was to determine the time‐dependent alterations in growth performance, histomorphology, digestive enzymes activities and the genes expression related to target of rapamycin (TOR) signalling pathway involved in hepatopancreas and intestine of Litopenaeus vannamei at 3, 6, 12, 18, 24 and 30 days after Aflatoxin B1 (AFB1) challenge....
Article
Our previous work showed that Lactobacillus pentosus HC-2 has high antibacterial and adhesion activity, and as a probiotic could improve the nutrients and immunomodulatory effects in the Litopenaeus vannamei farming. In order to further investigate the influence of HC-2 surface protein on its probiotic effects, the immune and digestion related gene...
Article
Aflatoxin B1 (AFB1) is a mycotoxin mainly produced by Aspergillus flavus and Aspergillus parasiticus contaminating food, feed ingredients and products of animal origin. In mammals, this toxin causes widespread organ-specific damage; it is immunotoxicity and could promote hepatotoxicity, alter intestinal functions and so on. In this study, we conduc...
Article
We investigated the intestinal microbiota in shrimp fed diets with five replacement levels (0, 25%, 50%, 75% and 100%) of fish meal by fish silage. An 8‐week feeding trail was conducted in 15 fibreglass tanks (400 L volume, three replications for each diet) with 50 shrimps (initial weight 0.26 ± 0.03 g) per tank. After 8‐week trial, shrimp fed diet...
Preprint
Full-text available
Our previously work showed that Lactobacillus pentosus HC-2 as probiotic could improve the growth performance, immune response, gut bacteria diversity and disease resistance of Litopenaeus vannamei. However, the probiotic mechanism of was not fully characterized. In the present study, histology and proteomic analysis was performed to explore the in...
Article
Tetraspanins belong to the transmembrane 4 superfamily (TM4SF), and play crucial roles in immune responses. In the present study, a novel tetraspanin gene (designated MmTSPAN) was cloned and characterized from the hard clam Meretrix meretrix. The complete cDNA sequence of MmTSPAN contained an open reading frame (ORF) of 816 bp, which encoded a prot...
Article
Intestinal microbiota plays a vital role in nutrient metabolism, pathogen resistance and development of immune system. Here, an Illumina-based method was employed to investigate the intestinal microbiota in white shrimp fed diets with five replacement levels (0, 10, 20, 30 and 40%) of fishmeal by fermented soybean meal (FSM). The results of growth...
Article
In order to understand the mediation function of surface proteins in probiotic effects executed by Lactobacillus pentosus HC-2 in midgut of Litopenaeus vannamei, the immune and digestion related enzymes and the transcriptome expression were analyzed after shrimp fed with normal HC-2 or with stripped surface proteins HC-2 by lithium chloride (LiCl)...
Article
The β-thymosin (Tβ) proteins participate in numerous biological processes, such as cell proliferation and differentiation, anti-inflammatory and antimicrobial mechanism. To date, Tβ proteins have been well studied in vertebrates, especially mammals. While limited Tβ or Tβ-like proteins have been reported in invertebrates. Moreover, rare information...
Article
Leucine-rich repeat (LRR)-only proteins are involved in innate immune responses through mediating protein-ligand or protein-protein interactions, yet the exact roles of most LRR-only proteins in invertebrates are not well documented. In the present study, a novel LRR-only protein (designated CfLRRop-7) was identified in Zhikong scallop Chlamys farr...
Article
Aflatoxins, especially aflatoxin B1 (AFB1), exert a substantial impact on the shrimp farming production, inducing disease with a gradual decline of reared shrimp stock quality and profitability, thus causing a significant problem in aquaculture systems. In order to investigate the influence of AFB1 on intestinal health and antioxidant system, we mo...
Article
As marine invertebrates, oysters lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune fun...
Article
Superoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF),...
Article
Aflatoxins are produced in aquaculture feed by the fungi Aspergillus flavus or Aspergillus parasiticus. Aflatoxins can cause severe health problems in shrimp, and thus reduce the yield and profitability of shrimp cultures. We sought to understand the interaction between shrimp metabolism and aflatoxin B1 (AFB1) by analyzing the expression of Litope...
Article
Biofloc meal is a sustainable protein source of aquafeed, which has been verified by several previous studies under experimental conditions. This study evaluated the potential for the replacement of fish meal (FM) with biofloc meal (BM) in the diet of Litopenaeus vannamei under practical conditions. Two isonitrogenous (430 g kg− 1) and isolipidic (...
Article
Mechanistic target of rapamycin (mTOR) is a central regulator of cell growth and metabolism including autophagy. The mTORC1 signalling pathway is a major regulator of autophagy in response to nutrient levels. The drug rapamycin induces autophagy in various cell types and species by specifically inhibiting the activity of mTOR. To identify the genes...
Article
The white shrimp Litopenaeus vannamei has been greatly impacted by Vibrio infection. In this study, we investigated the intestinal barrier response of L vannamei following challenge with Vibrio parahaemolyticus E1, by examining morphological changes and transcriptome expression levels. A total of 16,4420 unigenes were obtained from RNAseq data afte...
Article
Full-text available
Aflatoxin produced by Aspergillus flavus or Aspergillus parasiticus fungi during grain and feed processing and storage. Aflatoxins cause severe health problems reducing the yield and profitability of shrimp cultures. We sought to understand the interaction between shrimp immunity and aflatoxin B1 (AFB1), analyzing transcriptome expression, antioxid...
Article
Aflatoxin produced by Aspergillus flavus or Aspergillus parasiticus fungi during grain and feed processing and storage. Aflatoxins cause severe health problems reducing the yield and profitability of shrimp cultures. We sought to understand the interaction between shrimp immunity and aflatoxin B1 (AFB1), analyzing transcriptome expression, antioxid...
Article
In order to determine the impact of pathogenic Vibrio parahaemolyticus (Vp) on the hepatopancreas of Litopenaeus vannamei, shrimps with weights of (2.2±0.24) g were challenged with Vp at a concentration of 5×10⁷ CFU/mL for 36 h. The hepatopancreas tissues from the experimental and control groups were collected at 6 h, 12 h, 24 h, and 36 h after the...
Article
Full-text available
In this study, we investigated the interaction between Lactobacillus pentosus HC-2 and Vibrio parahaemolyticus E1 when cocultured in Litopenaeus vannamei in vivo and in vitro. Fluorescence microscopy was used to observe the distribution of these two strains, which were labeled with DAPI and CFDA-SE, respectively, in the gut of the shrimp. Fluoresce...
Article
Full-text available
The interactions of microbiota in guts play an important role in promoting or maintaining the health of hosts. In this study, in order to investigate and compare the effects of dietary supplementation with Lactobacillus pentosus HC-2 (HC-2), Enterococcus faecium NRW-2, or the bacteria-free supernatant of a HC-2 culture on the bacterial composition...
Article
Full-text available
Farmed sea cucumbers (Apostichopus japonicus) of the same age displayed significantly different body sizes and weights under the same farming conditions. To examine the gut bacterial diversity of sea cucumbers and whether the growth differences between them were related to intestinal microbiota, the bacterial species from intestinal samples of 30 f...
Article
To study the effect of low temperature stress on hematological parameters and HSP gene expression in the turbot (Scophthalmus maximus), water temperature was lowered rapidly from 18 to 1°C. During the cooling process, three individuals were removed from culture tanks at 18, 13, 8, 5, 3, and 1°C. Blood samples and tissues were taken from each indivi...
Article
In the shrimp challenge test the Vibrio dosage, infection route, and strain are considered as risk factors that result in mortality. Assessment of Vibrio/shrimp interactions, and disease dynamics following infection by Vibrio, are useful techniques needed for detailed studies on the control of risk factors. In this paper we used an application of t...
Article
Biodegradable N-maleyl gelatin (N-MAGEL) was synthesized with gelatin (GEL) and maleic anhydride (MAH) by acylation. With Bis-acrylamide (Bis) cross-linker, a series of cross-linked poly (N-isopropylacrylamide-co-N-maleyl gelatin) [P(NIPAAm-co-N-MAGEL)] hydrogels were prepared, and their lower critical solution temperature (LCST), water content, sw...
Article
The present study was conducted to elucidate the effects of copper exposure on the immune system and lipid metabolism of the Japanese scallop, Mizuhopecten yessoensis. Transcriptional levels of differentially expressed genes (DEGs)in M. yessoensis digestive gland tissue were analyzed using the deep-sequencing platform Illumina HiSeq(TM) 2000. In to...
Article
Different amounts of vitamin C were added to diets fed to juveniles (2.5 ± 0.15 g) of sea cucumber Apostichopus japonic u s (Selenka) in an attempt to reduce the stress response of specimens exposed to nitrite stress. A commercial feed was used as the control diet and three experimental diets were made by supplementing 1 000, 1 500, or 2 000 mg vit...
Article
Penaeidin is an antimicrobial peptide family found in penaeid shrimp. Penaeidin3 has a broad spectrum of antimicrobial activity and is most effective against certain bacterial species. In this study, the penaeidin3-2 gene was cloned from the Chinese shrimp, Fenneropenaeus chinensis. It was then transformed into rice plants using an Agrobacterium-me...
Article
The bivalve Mizuhopecten yessoensis has been greatly impacted by marine pollutants in northern China. To elucidate the toxicological mechanism of copper exposure on the immune system, we investigated differentially expressed genes (DEGs) and transcript abundance in M. yessoensis gill tissue using the deep-sequencing platform Illumina HiSeqTM 2000....
Article
The clotting protein (CP) plays important and diverse roles in crustaceans, such as coagulation and lipid transportation. A clotting protein was purified from the hemolymph of Chinese shrimp Fenneropenaeus chinensis (named as Fc-CP) with Q sepharose HP anion-exchange chromatography and phenyl sepharose HP hydrophobic interaction chromatography. Fc-...
Article
Peptides derived from shrimp hemocyanin have antimicrobial properties. This is the first report of hemocyanin cDNA (FCHc) cloned from Fenneropenaeus chinensis and recombinant expression of two C-terminal fragments. Based on sequence analysis of Fenneropenaeus chinensis hemocyanin FCHc, we subcloned two FCHc fragments by designing special primers. T...
Article
This research aimed to investigate the accumulation of Cadmium (Cd) and the antioxidant defense system (Superoxide Dismutase (SOD), Catalase (CAT) and Glutafhione Peroxidase (GPx)) in gill and digestive gland oiMizuhopecten yessoensis exposed to Cd (final Cd doses of 0, 0.05, 0.1 and 0.2 mg L-1) for 14 days. The uptake of Cd in both tissues via sea...
Article
Full-text available
In this study, the intestinal microbiota of Chinese shrimp (Fenneropenaeus chinensis) was examined by molecular analysis of the 16S rDNA. The aims of the study were to evaluate the use of molecular-based techniques, Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR–DGGE) and clone library analyses, and compare the results obtai...
Article
In this study, the intestinal microbiota of kuruma shrimp (Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp. on intestinal microbial diversity. Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp. amended fee...
Article
Using the LAMP method, a highly specific and sensitive detection system for genetically modified soybean (Roundup Ready) was designed. In this detection system, a set of four primers was designed by targeting the exogenous 35S epsps gene. Target DNA was amplified and visualized on agarose gel within 45 min under isothermal conditions at 65 degrees...
Article
A natural lectin from the plasma of the shrimp Fenneropenaeus chinensis was purified by singlestep affinity chromatography using fetuin-coupled agarose. The purified plasma lectin showed a strong affinity for human A/B/O erythrocytes (RBC), mouse RBC and chicken RBC. The hemagglutinating (HA) activity of the lectin was dependent on Ca(2+) and rever...
Article
A natural lectin from the serum of the shrimp Litopenaeus vannamei was purified to homogeneity by a single-step affinity chromatography using fetuin-coupled agarose. The purified serum lectin (named LVL) showed a strong affinity for human A/B/O erythrocytes (RBC), mouse RBC, chicken RBC and its haemagglutinating (HA) activity was specifically depen...

Citations

... KSPIs were named in reference to Kazal et al. (1948), who first isolated the pancreatic secretory trypsin inhibitor. After that, more KSPIs were studied in vertebrates and invertebrate species, such as mammals (Xu et al. 2019), birds (Bourin et al. 2011), fish (Kwon et al. 2018), shrimp (Wang et al. 2009(Wang et al. , 2021 Vargas-Albores and Villalpando 2012), insects (Brillard-Bourdet et al. 2006;Zheng et al. 2007;Kim et al. 2013;Yang et al. 2020), mollusks (Yu et al. 2017), coelenterates (Zhou et al. 2018), etc. Insects are the most diverse and widely distributed organisms. Compared with vertebrates, insects lack an acquired immune system, but have a very effective innate immune system composed of humoral immunity and cellular immunity. ...
... However, Lactobacillus and Cetobacterium are important beneficial bacteria in the intestine (104). For example, Lactobacillus can reduce the intestinal pH, which might contribute to overcoming pathogen challenges (105). Cetobacterium might be involved in the metabolism of the host and provide vitamin B12 (106). ...
... The biosynthesis of triterpenoids in G. lucidum depended on the biosynthesis of mevalonic acid and lanosterol (Fig. 5b). Lanosterol is one of the most important precursors of triterpenoids in G. lucidum [30,31]. The skeletons of triterpenoids were generated by the oxidation and cyclization reaction of hydroquinone [31]. ...
... In recent years, isothermal nucleic acid amplification technologies, such as recombinase-aided amplification (RAA; Piepenburg et al., 2006;Subsoontorn et al., 2020) and loop-mediated isothermal amplification (Nagamine et al., 2002), have promoted the development of nucleic acid amplification without thermal cyclers. RAA assay that only requires one primer set and can be reacted even at body temperature of operator (Wang et al., 2017) has been used for the detection of pathogens such as A. hydrophila (Qu et al., 2021) and Enterocytozoon hepatopenaei (Zhou et al., 2020), but this method has low sensitivity (Xiao et al., 2021) and the result is complicated to obtain (Zhou et al., 2020). ...
... vannamei), destroy the antioxidant system and increase susceptibility to pathogens. 117,118 For instance, in fish (e.g., sub-Antarctic Notothenioid fish Eleginops maclovinus), high stocking density (60 kg m −3 ) increases total α-amino acid content but decreases glycogen levels. 119 Thus, a metabolite such as inosine has been used as a general biomarker of stress in P. vannamei. ...
... Such studies have revealed genes and pathways involved in steroid regulation, vitellogenesis, lipid droplet synthesis, oocyte development, and maturation [3][4][5][6]. More research on embryogenesis during gestation in females has been carried out in mammals and other viviparous species [7][8][9]. However, research on viviparous teleosts is limited [10]. ...
... In some studies, cell-free extract or fermentation supernatant of Lactobacillus plantarum was also found to be beneficial to the host (Zheng et al., , 2020. Additionally, Lactobacillus pentosus HC-2 treated with lithium chloride (LiCl) to eliminate non-covalently bound surface proteins has an effect on probiotic actions in the shrimp midgut (Du et al., 2019). However, our study found that L. vannamei fed with live E. faecalis F7 preparation diets performed better in terms of weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR) when compared to that fed with a regular diet, dead bacteria, cell-free extract of E. faecalis F7 and E. faecalis F7 treated with LiCl ( Figure 1). ...
... fermented 75% soybean meal (FSBM) diet had a higher total hemocyte count and demonstrated the highest phenoloxidase (PO) activity (Lin and Chen 2022). Du et al. (2019b) denoted that L. pentosus could increase the immunomodulatory performance in the L. vannamei culture. ...
... At present, studies on the effects of AFB 1 on PRRs mainly focus on Toll-like receptors (TLRS) and nucleotide binding oligomerization domain-like receptors (NLRs). It was reported that AFB 1 evidently decreased mRNA expressions of TLR2, TLR4, and TLR7 in the small intestine of broilers [34]. We used different concentrations of AFB 1 to stimulate primary chicken embryo intestinal cells (100 µg/mL) and liver and kidney cells (40 µg/mL) for 12 h, and the results showed that TLR2 was significantly up-regulated in kidney cells, while there was no significant difference in other cells [23]. ...
... The primers of 338F (5′-ACTCCTACG GGAGGCAGCA-3′) and 806R (5′-GGACTACHVGGGT WTCTAAT-3′) were used, which were modified with 7 bp barcodes to distinguish the PCR products. PCR amplification was performed according to the reported protocol of Shao et al. [23]. PCR products were detected by agarose gel electrophoresis and subsequently purified using an Axy-Prep™ DNA gel extraction kit (Axygen, Hangzhou, China). ...