Katia Peñuelas-Urquides's research while affiliated with Mexican Institute of Social Security and other places

Publications (13)

Article
Full-text available
The emergence of multidrug-resistant (MDR) Mycobacterium tuberculosis strains threaten the control of tuberculosis. New antitubercular dihydrosphingosine analogs, named UCIs, have been evaluated in preclinical studies but their cellular and molecular mechanisms of action against M. tuberculosis are still unknown. The aim of this study was to evalua...
Article
Full-text available
Variants of 8q24 locus have been associated with prostate cancer (PCa) susceptibility. This study aims to analyze the genetic basis of PCa susceptibility in Mexican men by analyzing SNPs in the 8q24 locus for the first time. A case-control study was performed in 875 men recruited from the Mexican Social Security Institute, 326 patients with PCa, an...
Article
Background and aims To assess the relevance of the slow acetylator phenotype based on NAT2 genotypes, among patients with pulmonary tuberculosis (PTB) that developed hepatotoxicity after first-line tuberculosis treatment in a Northeastern Mexican population. Methods Ninety one PTB patients were included, 7 of them developed hepatotoxicity. NAT2 SN...
Article
Full-text available
Background: Mortality due to tuberculosis (TB) has increased due to the development of drug resistance, the mechanisms of which have not been fully elucidated. Our research group identified a low expression of lipF gene in Mycobacterium tuberculosis clinical isolates with drug resistance. The aim of this work was to evaluate the effect of lipase F...
Article
Tuberculosis drug resistance (DR) is a global problem that is not fully elucidated. Previously, overexpression of esxG and esxH genes was reported in a multidrug-resistant (MDR) Mycobacterium tuberculosis isolate compared with a reference H37Rv strain. To evaluate the roles of esxG and esxH in DR, analysis of their regulatory and coding sequences i...
Article
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Background and aims The impact of host genetic variation in susceptibility of tuberculosis is well documented. The vitamin D receptor gene (VDR) is a transacting transcription factor which mediates innate immune response by enhancing the expression of several antimicrobial peptides, including cathelicidin. An association between VDR polymorphisms w...
Chapter
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Since genomic data are widely available, many strategies have been implemented to reveal the function of specific nucleotides or amino acids in promoter regions or proteins, respectively. One of the methods most commonly used to determine the impact of mutations is the site‐directed mutagenesis using the polymerase chain reaction (PCR). There are d...
Article
Dystrophin Dp71, the smallest product encoded by the Duchenne muscular dystrophy gene, is ubiquitously expressed in all nonmuscle cells. Although Dp71 is involved in various cellular processes, the mechanisms underlying its expression have been little studied. In hepatic cells, Dp71 expression is downregulated by the xenobiotic β-naphthoflavone. Ho...
Article
Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations, and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA, and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant clinical isolate. Here, we have...
Article
The 2009 influenza A(H1N1) outbreak allowed the implementation of new epidemiologic surveillance tools in several countries around the world. A new molecular protocol with appropriate sensitivity and specificity using real-time RT-PCR was developed by the Centers for Disease Control and Prevention (CDC) to identify the pandemic 2009 influenza A (H1...
Article
Full-text available
The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600...
Article
Full-text available
Mycobacterium tuberculosis has developed resistance to anti-tuberculosis first-line drugs. Multidrug-resistant strains complicate the control of tuberculosis and have converted it into a worldwide public health problem. Mutational studies of target genes have tried to envisage the resistance in clinical isolates; however, detection of these mutatio...

Citations

... Papillary thyroid carcinoma susceptibility candidate 1 (PTCSC1) in locus 8q24 has been identified, by GWLA, in a family with PTC, benign thyroid nodules and melanoma, as a predisposing locus for PTC (45). More recent studies have also identified variants in this 8q24 locus associated with risk of prostate cancer, colorectal cancer, breast cancer, bladder cancer, stomach cancer, glioma and thyroid cancer (132)(133)(134). ...
... (40) Also, trying to prevent adverse events such as DIH, many authors have already proposed, in quite distinct populations, the development of a NAT2 genotyping test to be applied to patients with TB diagnosis previously to TB treatment. (41,42,43,44) In this way, different genotyping panels have been proposed, revealing greater accuracy of either five or seven-SNPs panels. (12,45) Beyond that, one research performed in the Brazilian population suggested the four-SNPs panel (c.191G>A, c.341T>C, c.590G>A, and c.857G>A) as the most appropriate test to predict the individual NAT2 acetylation status with accuracy up to 100%. ...
... Another STR resistance mechanism is likely related to lipF (Rv3487c), which encodes for a lipase with phospholipase C and carboxylesterase activities. The reduced expression of this gene is thought to contribute to the development of resistance to STR [65]. Moreover, the M. tuberculosis homocysteine synthase MetC (Rv3340), a key enzyme of the methionine synthesis pathway, was hypothesized to promote STR resistance. ...
... [9] Knockdown (antisense) strategy has been well established for identifying the pathogenic and virulent determinants in mycobacteria. [11,12] Therefore, the present study was conducted to validate the role of hadC in M. fortuitum biofilm formation using knockdown strategy. For this, we constructed and characterized M. fortuitum hadC knockdown and over-expressing strains. ...
... Many genes associated with vitamin D metabolic pathway were involved in the host susceptibility to persistent TB infection in which vitamin D receptor (VDR) had been well studied [18][19][20]. Vitamin D exerted its biological functions through binding with VDR; hence, VDR variant might contribute to the TB development by resulting in diminished function of vitamin D. The study by Hu et al. demonstrated a significant association between VDR rs11574143, rs11168287, rs11574079 polymorphisms and PTB susceptibility in the Chinese population [21]. Besides VDR, other genes involved in vitamin D pathway, including CYP24A1, CYP27A1, CYP27B1, CYP2R1, GC, DHCR7, might also be associated with TB susceptibility [22]. ...
... A simple mutation method is based on the amplification of double-stranded DNA from the template DNA using complementary oligonucleotides, or primers, carrying the mutation of interest while multiple mutations either obtain the mutation after several rounds of this process or can obtain them simultaneously in one reaction. 18 In a PCR small amounts DNA are amplified or copied using a thermocycler. There are three steps to a PCR reaction: denaturation, where the DNA is heated and denatures becoming single-stranded, annealing where the primers, containing the mutation, bind to flanking regions of the DNA, and extension where DNA polymerase extends the 3' end of each primer along the template strand. ...
... (Galvagni et al., 1998;Zanotti et al., 2015). Similarly, YY1 was shown to negatively regulate dystrophin Dp71, the smallest protein encoded by the Dmd gene, in hepatic cells (Peñuelas-Urquides et al., 2016). Finally, YY1 appeared to be a potential target of GCN5 acetylation because YY1 was previously shown to be a target of acetyltransferases p300 and p300/CBP-associated factor (PCAF; Yao et al., 2001). ...
... In this regard, numerous studies demonstrate the value of WGS in predicting DR-M.tb phenotypes from the M.tb genome sequenced directly from clinical specimens (e.g., sputum) (Groschel et al., 2018;Cohen et al., 2019b) (Table 2). Importantly, WGS has provided new insights into the complex underlying molecular mechanisms of M.tb drug resistance thanks to its nucleotide-level resolution (Gonzalez-Escalante et al., 2015;Yu et al., 2015;Coll et al., 2018) ( Table 2). Of interest, studies of M.tb clinical isolates collected from the same patient at a single time point (parallel isolates) or at a different time points (serial isolates) during active TB disease progression and/or drug treatment, revealed within-host M.tb genomic diversity and microevolution events (O'Neill et al., 2015;Ley et al., 2019;. ...
... In addition the inclusion of positive and negative controls is essential to assess accurate results and success of each RT-PCR assay. For infectious viruses that require high biocontainment it may not always be possible for all laboratories to have access to viral RNA and hence using a plasmid to synthesis RNA controls can circumvent the requirement for infectious virus and can provide a standardised control that could readily be shared with other laboratories (Bermudez de Leon et al., 2013;Whiley et al., 2010). However, one drawback is the possibility of false positive results. ...