Kathrin Büttner’s research while affiliated with University of Giessen and other places

Morphometric measurements of the toe serve as a reference for the objective assessment of the presence of a pathological condition and as a guide for the correct trimming of the hooves. Therefore, the anatomical differences between horses and donkeys oblige an autonomous database with reference values for the donkey. This study is the first to provide readings of the distal hind limb on radiographs of donkeys. Radiographs of the distal hind limb in two projections of 41 generally sound donkeys without lameness in walk were taken after previous hoof trimming and preparation according to a standardized radiographic procedure. A total of 16 lengths and 9 angles were measured and statistically analyzed in each hoof. The dorsal hoof wall is on average 70 to 80 mm long and angled at 55°; the coffin bone runs parallel to the hoof wall. The average founder distance is 17 mm, the mean sole thickness is 14 mm. The heel angle averages 52°, which is slightly flatter than the hoof wall angle. In conclusion, the morphometric measurements confirm the difference in normal anatomy between horses and donkeys and correlations with weight and height are observed.

Introduction In the diagnostics of orthopedic diseases in the horse, diagnostic imaging often plays a decisive role. Cone beam computed tomography (CBCT) imaging is used in both human and small animal medicine and becoming increasingly popular. To see whether CBCT imaging can be useful in the diagnosis of orthopedic diseases of the carpal region of the horse and to explore possible limitations we compared CBCT images with multidetector computed tomography (MDCT) images of the carpal region of equine cadaveric specimens. Materials and methods Twenty-eight forelimbs from fifteen horses, slaughtered for reasons unrelated to this study, were examined. Native and contrast enhanced CBCT and MDCT scans were performed. Anatomical structures were blindly evaluated by three independent experienced observers using a visual scoring system previously reported and adapted to the equine carpal region. A descriptive evaluation was carried out as well as Spearman’s rank correlation and interobserver agreement was shown by percent agreement (PA). Results Visualization of osseous structures was excellent in both MDCT and CBCT. Articular cartilage could only be assessed in contrast enhanced scans whereby MDCT showed a slightly better visualization than CBCT. Soft tissue structures were generally difficult to assess. An exception were the medial and lateral palmar intercarpal ligament, which could not be visualized in native but were well visualized in contrast enhanced scans in both MDCT and CBCT images. Discussion/conclusion For the evaluation of osseous structures and some intraarticular ligaments after contrast enhancement, CBCT serves as a reliable diagnostic imaging modality for the equine carpal region. However, soft tissue structures and cartilage are imaged more reliably using MDCT.

Background/Objectives: The dietary fat concentration and fatty acid profile can influence various aspects of the feline organism. This study examined their effects on the urine composition, apparent nutrient digestibility, and selected blood variables. Methods: Ten healthy adult cats (46.6 ± 14.1 months old, initial body weight 4.99 ± 0.91 kg) received a low-fat basic diet with or without the addition of sunflower oil, fish oil, or lard in a randomized crossover design. The oil and lard were added to the daily amount of food at 0.5 or 1 g/kg body weight of the cats. At the end of each 3-week feeding period, urine, feces, and fasting blood samples were collected. Results: The results demonstrated only small effects of the dietary fat concentration and source on the urine composition of the cats. In addition, the apparent nutrient digestibility was unaffected by the dietary treatments. The supplementation with fish oil, but not sunflower oil or lard, lowered the triglycerides and increased the total and low-density lipoprotein cholesterol concentrations in the plasma of the cats (p < 0.05). However, these blood values were within the physiological reference ranges among all groups. Conclusions: It can be concluded that the dietary fat content and fatty acid profile did not adversely affect the urine composition or nutrient digestibility in healthy adult cats. The lipid metabolism of the animals was modulated by the supplementation with fish oil, a relevant source of n-3 fatty acids. The observed triglyceride-lowering effect should be further investigated in clinical studies.

Background Dogs with internal hydrocephalus do not necessarily have high intraventricular pressure (IVP). Hypothesis/Objectives Not all reported MRI findings indicate high IVP and some clinical signs might be associated with elevated IVP and syringomyelia. Animals Fifty‐three dogs. Materials and Methods Cross‐sectional study. Clinical signs and MRI findings were evaluated for an association of IVP >12 mm Hg and syringomyelia. Results High IVP was associated with obtundation OR 4.64 (95% CI 1.27‐16.93) (P = .02), head tilt OR 6.42 (95% CI 1.08‐37.97) (P = .04) and nystagmus OR 8.24 (95% CI 1.44‐47.07) (P = .02). Pain was associated with syringomyelia OR 3.4 (95% CI 0.98‐11.78) (P = .05). The number of affected ventricles was associated with high IVP OR 2.85 (95% CI 0.97‐8.33) (P = .05) and syringomyelia OR 12.74 (95% CI 2.93‐55.4) (P = .0007). Periventricular edema OR 24.46 (95% CI 4.54‐131.77), OR 7.61 (95% CI 1.91‐30.32) (P < .0002, P = .004) and signal void sign OR 17.34 (95% CI 4.01‐74.95), OR 4.18 (95% CI 1.16‐15.02) (P < .0001, P = .03) were associated with high IVP and syringomyelia. The probability for syringomyelia is lower with disruption of the internal capsule OR 0.19 (95% CI 0.05‐0.72) (P = .01) and higher VBR OR 0.25 (95% CI 0.1‐0.63) (P = .004). Conclusions and Clinical Importance Previously reported MRI findings are not predictive of high IVP. Clinical signs and MRI findings should be used to make a diagnosis of internal hydrocephalus in dogs with or without high IVP.

Background Dystocia is rare in horses, but is life-threatening for mares and foals. Therefore, veterinary research depends on up-to-date data to optimise equine dystocia management. In addition, knowledge of the prognosis of equine dystocia is necessary to advise animal owners. This retrospective study of equine dystocia aimed to enrich existing datasets with up-to-date information. For the first time, the focus was on the causes of dystocia that occurred alone or in combination. Over a period of 10 years, 72 cases of dystocia were analysed using a standardised, predetermined diagnosis code. Results Of the 72 cases of dystocia, an isolated cause of dystocia was identified in 37 cases (51.4%) and 35 mares showed a combination of two or more causes (48.6%). Foetal causes were significantly more frequent origin of dystocia (66/69) than maternal causes (3/66) ( P < 0.0001). Incorrect posture of the foetal forelimbs and head was the most common combination at 25.7%. The most common isolated cause of dystocia was incorrect posture of the foetal forelimbs (18.9%). A foetotomy was performed in 68% of cases. A caesarean section or an extraction was performed in 13.9% of the cases. Three mares died before any obstetric care was provided. Nine mares (12.5%) were not discharged alive. 73 foals were delivered of which 55 were already dead before veterinary treatment began. In relation to the total number of births in which the foetus was alive at the start of obstetric care, the foetal mortality rate was 61.1% and 88.9% by the time the mare was discharged. Conclusions It has been demonstrated for the first time that multiple causes of dystocia in horses are almost as common as isolated causes of dystocia. Neonatal mortality remains high, indicating that the timely detection and treatment of dystocia has the highest priority.

Introduction Stallion castration is a standard procedure with a risk of post-surgical complications. Castration induces an acute phase response (APR). Serum Amyloid-A (SAA) is a well-studied major acute phase protein (APP), that has been shown to be a good marker for the development of post-surgical complications. The current gold standard for reducing the APR after castration is Flunixin-Meglumin, which is a non-steroidal anti-inflammatory drug (NSAID) inhibiting COX1/2. In contrast, Traumeel LT ad us. vet. can modulate the APR by induction of the inflammation resolution. The aim of this study was to compare the effect of Flunixin-Meglumin and Traumeel LT ad us. vet. on the acute phase response. Material and methods A total of 60 stallions were recruited and 54 stallions entered the study with 27 stallions in each treatment group. The stallions were treated pre- and postoperatively with either Flunixin-Meglumin (FL) or with Traumeel LT ad us. vet. (TR). Blood was taken before and 24 h, 48 h and 72 h after castration. The following main parameters were assessed: SAA, fibrinogen, iron, white blood cells, neutrophils, Interleukin1ß, and cortisol. Wound healing and pain were assessed at 8 time points. Results The main variable SAA was increased after surgery reaching a mean value of 122 µg/ml in the FL group and a mean SAA of 226 µg/ml in the TR group 48 h after surgery, reaching a significant difference only at the 24 h timepoint (p = 0.03). All stallions had the highest pain summary score 8 hours after surgery, with decreasing values thereafter. The pain scores were not statistically different at any time point. In the FL group five stallions developed a suture dehiscence compared to only one stallion in the TR group (p = 0.001). Discussion Within the limitations of this study, Traumeel LT ad us. vet. seems to have proresolving effects on the inflammation induced by surgery making it a valuable treatment to reduce the APR induced by castration. Due to its different mode of action, Traumeel LT ad us. vet. might be an alternative treatment option if gastrointestinal side effects or renal side effects of NSAIDs should be avoided. Further studies are needed combining Traumeel LT ad us. vet. and Flunixin.

Background Feeding milk substitutes with low iron content or whole milk without iron supplementation is considered a major factor in developing iron-deficiency anemia in neonatal dairy calves. Young calves are often supplemented with iron dextran injections on the first day of life to prevent anemia. However, the effects of preventive treatment and the presence of disease on serum iron (Fe) concentrations, serum ferritin levels, and hematological blood parameters during the early neonatal stages have not been examined in detail. Therefore, we examined and evaluated the effects of iron dextran injections and health status on the development of hematocrit (Ht), red blood cells (RBC), hemoglobin concentration (Hb), erythrocyte indices (mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration), Fe, and serum ferritin concentrations in dairy calves within the first 10 days of life. The suitability of serum ferritin as a reliable indicator of anemia in very young calves was evaluated by correlating ferritin concentrations with known laboratory diagnostic parameters of anemia. Results Iron supplementation significantly increased Fe levels (P = 0.048) but did not affect serum ferritin levels in neonatal calves. Fe concentrations were significantly lower in diseased than healthy calves (P = 0.0417). Iron supplementation significantly affected the health status, as observed in Ht (Ptreat=0.0057; Phealth=0.0097), RBC (Ptreat=0.0342; Phealth=0.0243), and Hb (Ptreat=0.0170; Phealth=0.0168). Serum ferritin levels did not significantly correlate with Fe levels. Both groups showed marked differences in ferritin levels, with the highest levels measured on day 2. Fe concentrations showed weak negative correlations with Hb and Ht levels on day 3 (ρ=-0.45; P = 0.0034 and ρ=-0.045; P = 0.0032, respectively). RBC count showed strong positive correlations with Hb and Ht levels (ρ = 0.91 and ρ = 0.93; P < 0.001). Conclusion Iron dextran injections increased Fe concentrations but reduced Ht level, RBC count, and Hb level. The presence of diseases led to a reduction in Fe and higher values of Ht, RBC, and Hb in moderate disease than in severe disease. Due to physiological fluctuations during the first 3 days of life, serum ferritin level seems unuseful for evaluating iron storage before day 4 of life.

Wild boar trapping has been used as a management tool to control wild boar populations. However, it is increasingly criticized due to animal welfare concerns. While cortisol levels have been used to assess trap-related stress in wild boar, data on trap-related injuries and behavioral data are scarce. We aimed to evaluate three different corral-style traps for wild boar according to available mammal trapping standards to investigate and refine animal welfare in wild boar trapping. We examined 138 wild boars captured and killed by head shot in 27 capture events. Traps were closed by remote control only if the complete group were trapped. The behavior of the animals in the trap and during culling was recorded on video. All wild boars were examined and a pathological and radiological examination of the heads for trap- and shot-related injuries followed. Trap-related injuries occurred in 33% of the animals with superficial mild skin defects to skull fractures. One out of three traps met all the set requirements. A wire-meshed trapping system failed all. After installing an incomplete barrier in the center of the trap to slow down trapped animals, the fracture rate in one trap type was significantly reduced by 29% (p < 0.05). Our data showed that the type of trap (p = 0.007) and the number of animals trapped at once (p = 0.002) had a significant influence on the number of escape attempts. Trapping larger groups reduced the escape attempts. We emphasize the importance of an accurate pathological examination to evaluate animal welfare in traps and call for adjusting the injury categories listed in the standards and make a proposal for wild boar live trapping.