Katherine Y. Leiner's scientific contributions

Citations

... The protein and nucleic acid content of the purified LPS was less than 1%. Protein content was estimated colorimetrically [29] and nucleic acids were determined spectrophotometrically. Phosphate, 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) and hexosamine contents of the LPS were 140.2, 98.6, and 288.5 nmol/mg, respectively. ...
... Assay of alkaline phosphatase (ALP) activity. ALP activity was determined using p-nitrophenyl phosphate (p-NPP) as substrate 25 . In brief, osteoblasts were plated (10,000 cells per well) in a 12-well culture plate. ...
... The monosaccharide composition and linkage types in serotype 28F and 28A CPS repeat unit were determined by GC and GC-MS after dephosphorylating by HF using methods described previously (Li et al., 2019). The content of total phosphate was determined by colorimetric analysis according to Lowry et al. (Lowry, Roberts, Leiner, Wu, & Farr, 1954). The absolute configuration of monosaccharides was determined by GLC (Gerwig, Kamerling, & Vliegenthart, 1979), by comparison with authentic standards of the acetylated O-[(R)-2-Octyl] glycoside after methanolysis (0.5 M HCl/MeOH, 85 • C, 3.5 h), butanolysis (2 M HCl/(R)-2-Octanol, 65 • C, 4 h) and peracetylation (85 • C, 10 min). ...