Karin S. Bruwelheide’s research while affiliated with Florida Museum of Natural History and other places

The authors report on ancient DNA data from two human skeletons buried within the chancel of the 1608–1616 church at the North American colonial settlement of Jamestown, Virginia. Available archaeological, osteological and documentary evidence suggest that these individuals are Sir Ferdinando Wenman and Captain William West, kinsmen of the colony's first Governor, Thomas West, Third Baron De La Warr. Genomic analyses of the skeletons identify unexpected maternal relatedness as both carried the mitochondrial haplogroup H10e. In this unusual case, aDNA prompted further historical research that led to the discovery of illegitimacy in the West family, an aspect of identity omitted, likely intentionally, from genealogical records.

Human bone fragments were discovered during archaeological monitoring of earth moving on Manassas National Battlefield Park in Virginia. Later mitigation recovered bones in situ—two skeletons and seven amputated limbs. Interdisciplinary research affords an unusually detailed level of interpretation, including identification of the remains as Union soldiers wounded during the Battle of Second Manassas (28–30 August 1862). The reconstructed narrative includes military and personal markers of identity, as well as causes of death and injury, and establishes a window from 1 to 6 September 1862 when the pit was dug. Records of Union surgeons make future personal identification of the amputated limbs possible and confirm the pit’s location as a key treatment center after Second Manassas, a battle that marked an inflection point for combat military medicine by highlighting the urgent need for improved systematic recovery and treatment of the wounded.

Planned restoration of a 19th-century burial vault in Jones County, North Carolina, associated with the Foscue family, led to its excavation in 2010. Family lore and related documents identify only three family members buried in the vault, but nine individuals are represented by the remains. Possible identities of the unnamed individuals are explored using biological data, family documents, and family lore. The invisibility of some individuals in the historical record reflects a period of shifting family structures, high female and infant mortality, and the effects of social status on documentation and remembrance.

The 17th-century colonization of North America brought thousands of Europeans to Indigenous lands in the Delaware region, which comprises the eastern boundary of the Chesapeake Bay in what is now the Mid-Atlantic region of the United States. The demographic features of these initial colonial migrations are not uniformly characterized, with Europeans and European-Americans migrating to the Delaware area from other countries and neighboring colonies as single persons or in family units of free persons, indentured servants, or tenant farmers. European colonizers also instituted a system of racialized slavery through which they forcibly transported thousands of Africans to the Chesapeake region. Historical information about African- descended individuals in the Delaware region is limited, with a population estimate of less than 500 persons by 1700 CE. To shed light on the population histories of this period, we analyzed low-coverage genomes of 11 individuals from the Avery’s Rest archaeological site (circa 1675–1725 CE), located in Delaware. Previous osteological and mitochondrial DNA (mtDNA) sequence analyses showed a southern group of eight indivi uals of European maternal descent, buried 15–20 feet from a northern group of three individuals of African maternal descent.5 Autosomal results further illuminate genomic similarities to Northwestern European reference populations or West and West-Central African reference populations, respectively. We also identify three generations of maternal kin of European ancestry and a paternal parent-offspring relationship between an adult and child of African ancestry. These findings expand our understanding of the origins and familial relationships in late 17th and early 18th century North America.

The 17th-century colonization of North America brought thousands of Europeans to Indigenous lands in the Delaware region, which comprises the eastern boundary of the Chesapeake Bay in what is now the Mid- Atlantic region of the United States.1 The demographic features of these initial colonial migrations are not uni- formly characterized, with Europeans and European-Americans migrating to the Delaware area from other countries and neighboring colonies as single persons or in family units of free persons, indentured servants, or tenant farmers.2 European colonizers also instituted a system of racialized slavery through which they forcibly transported thousands of Africans to the Chesapeake region. Historical information about African- descended individuals in the Delaware region is limited, with a population estimate of less than 500 persons by 1700 CE.3,4 To shed light on the population histories of this period, we analyzed low-coverage genomes of 11 individuals from the Avery’s Rest archaeological site (circa 1675–1725 CE), located in Delaware. Previous osteological and mitochondrial DNA (mtDNA) sequence analyses showed a southern group of eight individ- uals of European maternal descent, buried 15–20 feet from a northern group of three individuals of African maternal descent.5 Autosomal results further illuminate genomic similarities to Northwestern European refer- ence populations or West and West-Central African reference populations, respectively. We also identify three generations of maternal kin of European ancestry and a paternal parent-offspring relationship between an adult and child of African ancestry. These findings expand our understanding of the origins and familial relationships in late 17th and early 18th century North America.

Three unidentified lead coffins were discovered at Historic St. Mary’s City during a remote sensing and archaeological excavation of a 1660s brick chapel. Their investigation led to a re-examination of two additional lead coffins from a nearby brick vault constructed in 1694 and believed to house the bodies of Maryland’s first royal governor and his wife. These five rare coffins and their contents, examined by an interdisciplinary team including nuclear physicists, engineers, a pollen specialist, geologist, archaeologists, and forensic anthropologists, provide information on seventeenth-century identities, colonial life, and behaviours lost over time. Previously unknown aspects of diet, health, and childcare are revealed through skeletal and chemical analyses. The information from these high-status individuals serves as a contrast and comparison to other, less affluent individuals buried at St. Mary’s and throughout the Chesapeake region.

Nearly four decades ago, a highway expansion project resulted in the excavation of 35 unmarked graves at Catoctin Furnace, an industrial ironworking village in western Maryland. Initial analysis identified the remains as Africans or African Americans associated with the late 18th- and early 19th-century operation of the ironworks. Renewed efforts to learn more about these poorly documented individuals and connect the site’s untold past to present generations through heritage tourism, prompted reanalysis of the skeletons. Updated assessments of demography and pathology, along with new analyses including heavy metals and carbon and nitrogen stable isotopes, elucidate the life histories of these early laborers and their involvement in furnace operations. Some data derived from recent testing differentiate the Catoctin Furnace individuals from their plantation-based contemporaries in the mid-Atlantic, suggesting regional differences in diet and possible occupational exposure to toxins.

Objectives: Stable isotope values for historic period human remains from Elmina, Ghana, are compared to isotope data from 18th- and 19th-century North American sites as a test case for examining African origins and identifying first generation Africans in the Mid-Atlantic region of the United States. Materials and methods: Stable carbon, nitrogen, and oxygen isotope values were measured in skeletal remains. Values from the cosmopolitan port city of Elmina provide the first available reference data from Africa during this time period and region. These values serve as a proxy for West African groups in general which are statistically compared to Euro-Americans and African Americans. Results: Elmina carbon isotope values are relatively higher than those of North Americans, and African Americans show greater statistical similarity to West Africans. Elmina nitrogen isotope values are higher than those of North Americans. Elmina oxygen isotope values are notably higher than those in all Mid-Atlantic North American sites in this study. Discussion: Similarity in carbon isotope values between Elmina and African Americans suggests commonalities in food availability or food preferences between these groups. Elevated nitrogen isotope values in Elmina individuals support the documented reliance of the local population on marine dietary resources at this coastal port. While carbon and nitrogen isotopes provide insight into foodways, oxygen isotope data, sourced from drinking water, provide better geographical information. The higher oxygen values from Elmina not only differentiate this group from North American Mid-Atlantic sites, but also make it possible to identify outliers at these sites as potential recent arrivals from West Africa.

Objectives: Ancient DNA (aDNA) and standard osteological analyses applied to 11 skeletons at a late17th to early18th century farmstead site in Delaware to investigate the biological and social factors of settlement and slavery in colonial America. Materials and methods: Osteological analysis and mitochondrial DNA (mtDNA) sequencing were conducted for all individuals and the resulting data contextualized with archaeological and documentary evidence. Results: Individuals of European and African descent were spatially separated in this colonial cemetery. The skeletal remains exhibited differences in osteological features and maternal genetic ancestry. A specific mtDNA haplotype appeared in a subset of the European-descended individuals suggesting they were maternally related. Individuals of African descent were not maternally related, and instead showed a diversity of haplotypes affiliated with present-day Western, Central, and Eastern regions of Africa. Discussion: Along with the bioarchaeological and documentary evidence, the aDNA findings contribute to our understanding of life on the colonial Delaware frontier. Evidence of maternal relatedness among European-descended individuals at the site demonstrates kin-based settlements in 17th century Delaware and provides preliminary identifications of individuals. The maternal genetic diversity of the individuals with African descent aligns with the routes of the trans-Atlantic slave trade but broadens our understanding of the ancestries of persons involved in it. Burial positioning, osteological pathology, and lack of maternal kinship among individuals of African descent provide tangible evidence for the emergence of racialized labor and society in Delaware during the late17th century.