Karen Zinzius's research while affiliated with University of Münster and other places

... reinhardtii, a consequence of PsaH regulation would be the differential binding of Lhca2. There is evidence that PSI oligomerization through PSI dimer formation could regulate electron partitioning at the PSI acceptor side [38]. It is proposed that, under anoxia, PSI remodeling and dimer formation channel electron efficiently to hydrogenase, thereby modulating electron partitioning in favor of H2 production, which in turn increases the sink capacity for photosynthetic electrons. ...

... According to the MS/MS fragmentation analyses, the vast majority of LacNAc-substituted N-glycans comprised one and two antennae, except for a single tri-antennary complex N-glycan (OE59) observed at m/z 3952. Three N-glycans (OE40, 55 and 57) were substituted by the LacNAc 2 repeat, but the vast majority of LacNAc motifs were substituted by both Fuc and GalNAc that define the A-group antigen (OE33, 39,41,44,45,[47][48][49][51][52][53][54][55][56][57][58][59] and for some of them, an additional Fuc on the GlcNAc that defines an ALe b antigen (OE44, 49 56-58). In contrast to C.gigas, seven N-glycans (OE29, 31, 34-36, 43, 52) were characterized by the presence of a terminal Fucα1_2Galβ1-3GlcNAc motif, owing to an intense Z-ion at M+Na-410, resulting from the fragmentation of the Galβ1-3GlcNAc linkage, coupled to B-fragment ion of Fucα1,2Gal1-3GlcNAc at m/z 660, as exemplified for OE35 ( Figure 4J), which strongly suggests the presence of terminal type-1 H-antigen in those N-glycans. ...

... Besides CBC regulation, Chlamydomonas TRXs participate in the maintenance of ROS homeostasis through reduction of PRXs and GPXs (Charoenwattanasatien et al., 2020;Dayer, Fischer, Eggen, & Lemaire, 2008;Goyer et al., 2002), and may also play a role in hydrogen peroxide signaling through regulation of catalase (Shao, Beck, Lemaire, & Krieger-Liszkay, 2008). In addition, TRXs regulate chloroplast redox homeostasis through redox-dependent activation of NADP-malate dehydrogenase (NADP-MDH). ...

... trimannosyl arm of C. reinhardtii or partly in the core xylosylation of C. reinhardtii (Schultz et al., 2018;Oltmanns et al., 2019). Such XT activity has never been studied so far. ...

... described in(Charoenwattanasatien et al., 2018)) was reduced or oxidized with 1 mM DTT or H 2 O 2 560 for 1 h at RT. The reducing/oxidizing agent was washed out over a G25 column (GE Healthcare) 561following the manufacturer's instructions using 30 mM MOPS, 100 mM KCl, pH 7.2 as buffering 562 agent. ...

... CrCRX harbors four Ca 2+ -binding EF hands and a TRX domain. 85 Binding of Ca 2+ to the EF hands is a prerequisite for the TRX enzymatic activity of CRX. C. reinhardtii strains where CRX expression was strongly downregulated showed altered photosynthetic electron transfer and were affected in the oxidative stress response. ...