Kang-Jin Jeong's research while affiliated with Korea Research Institute of Bioscience and Biotechnology KRIBB and other places
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Publications (77)
Background: Titanium is commonly used in blood-exposed medical devices because it has superior blood compatibility. Mycophenolic acid inhibits the proliferation of vascular smooth muscle cells. This study examined the effect of a non-polymer TiO 2 thin film–coated stent with mycophenolic acid in a porcine coronary overstretch restenosis model.
Meth...
Due to the inconsistent fluctuation of blood supply for transfusion, much attention has been paid to the development of artificial blood using other animals. Although mini-pigs are candidate animals, contamination of mini-pig T cells in artificial blood may cause a major safety concern. Therefore, it is important to analyze the cross-reactivity of...
In recent decades, many studies on the treatment and prevention of pancreatic cancer have been conducted. However, pancreatic cancer remains incurable, with a high mortality rate. Although mouse models have been widely used for preclinical pancreatic cancer research, these models have many differences from humans. Therefore, large animals may be mo...
Clostridium perfringens is ubiquitous in the environment and the gastrointestinal tract of warm-blooded animals. While part of the gut microbiome, abnormal growth of C. perfringens causes histotoxic, neurologic, and enteric diseases in a variety of animal species, including humans, due to the production of toxins. There is extremely limited informa...
Study of interactions between the nervous system and immunity offers insights into the pathogenesis of Parkinson's disease (PD) and potential therapeutic strategies for neurodegenerative diseases. Studies on rodents have revealed regulatory mechanisms of microglial activation and T lymphocyte recruitment in PD. However, the mechanisms underlying ch...
Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental com...
Nonhuman primate models are valuable in biomedical research. However, reference data for clinical pathology parameters in cynomolgus and rhesus monkeys are limited. In the present study, we established hematologic and biochemical reference intervals for healthy cynomolgus and rhesus monkeys anesthetized with ketamine hydrochloride. A total of 142 c...
Chronic inflammatory enteric diseases occur commonly in humans and animals, especially in captive bred macaques. However, information about the etiology of idiopathic chronic inflammatory diarrhea in cynomolgus monkeys is limited. In this paper, we reported the unusual case of idiopathic chronic diarrhea in a captive cynomolgus monkey based on micr...
The developmental competence of in vitro matured oocytes is still lower than that of the in vivo matured oocytes due to precocious meiotic resumption and inappropriate cytoplasmic maturation. Although numerous efforts have been attempted to accomplish better in vitro maturation (IVM) condition, only limited progress has been achieved. Thus, a curre...
Pigs are often selected for large animal models including for neuroscience and behavioral research, because their anatomy and biochemistry are similar to those of humans. However, behavioral assessments, in combination with objective long-term monitoring, is difficult. In this study, we introduced an automated video tracking system which was previo...
The function of microglia/macrophages after ischemic stroke is poorly understood. This study examines the role of microglia/macrophages in the focal infarct area after transient middle cerebral artery occlusion (MCAO) in rhesus monkeys. We measured infarct volume and neurological function by magnetic resonance imaging (MRI) and non-human primate st...
This study evaluated the effect of a novel triple drug-eluting stent containing antithrombotic abciximab, antioxidative alpha-lipoic acid (ALA), and antiproliferative sirolimus in a porcine model. The coronary arteries of pigs were randomized into three groups: triple drug-eluting stent (TES; n=10), sirolimus-eluting stent (SES; n=10), and bare met...
Mitochondria continuously fuse and divide to maintain homeostasis. An impairment in the balance between the fusion and fission processes can trigger mitochondrial dysfunction. Accumulating evidence suggests that mitochondrial dysfunction is related to neurodegenerative diseases such as Parkinson's disease (PD), with excessive mitochondrial fission...
Microorganisms play important roles in obesity; however, the role of the gut microbiomes in obesity is controversial because of the inconsistent findings. This study investigated the gut microbiome communities in obese and lean groups of captive healthy cynomolgus monkeys reared under strict identical environmental conditions, including their diet....
Background
Gross anatomy and sectional anatomy of a monkey should be known by students and researchers of veterinary medicine and medical research. However, materials to learn the anatomy of a monkey are scarce. Thus, the objective of this study was to produce a Visible Monkey data set containing cross sectional images, computed tomographs (CTs), a...
A bacterial strain belonging to the genus Atopobacter was isolated from a vaginal swab from a crab-eating macaque ( Macaca fascicularis ). Here, we report the draft genome sequence of this strain, AH10.
Successful production of transgenic pigs requires oocytes with a high developmental competence. However, cumulus-oocyte complexes (COCs) obtained from antral follicles have a heterogeneous morphology. COCs can be classified into one of two classes: class I, with five or more layers of cumulus cells; and class II, with one or two layers of cumulus c...
The neutralizing antibody responses by a formalin-inactivated EV71 vaccine in monkey.
(XLSX)
Enterovirus 71 (EV71) is a major etiological agent of various public health issues, particularly in the Asia-Pacific region. EV71 causes hand-foot-and-mouth disease (HFMD) and is associated with serious neurological disorders in young children. A formalin-inactivated EV71 candidate vaccine (KCDC-HFMDV1-EV71) based on the C4 subgenotype was previous...
Tyrosinase is a copper-containing enzyme that regulates melanin biosynthesis and is encoded by the tyrosinase (TYR) gene. Previous studies demonstrated that mutations in TYR could lead to oculocutaneous albinism type 1 (OCA1) owing to the failure of melanin formation. Although a previous study found that albinism in the rhesus monkey was derived fr...
Despite the application of numerous supplements to improve in vitro culture (IVC) conditions of mammalian cells, studies regarding the effect of fetal bovine serum (FBS) on mammalian early embryogenesis, particularly in relation to redox homeostasis, are lacking. Herein, we demonstrated that early development of in vitro-produced (IVP) porcine embr...
Effects of FBS or antioxidant supplementation during the early IVC phase on ROS generation and developmental competence of porcine PA embryos.
(A) Fluorescence microscopy of 1-cell embryos treated with CM-H2DCFDA after 6 h of cultivation in the presence or absence of either FBS or GSH (0.5 and 1.0 μM) for 0–2 days of IVC (left panel) and quantifica...
Effect of FBS treatment during early IVC phase on early development and cell number of blastocysts in porcine PA embryos.
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Effect of FBS supplementation timing on the developmental competence of porcine PA embryos
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Primer sequences used for semi-qRT-PCR and qRT-PCR.
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Effect of FBS and glutathione treatment during the early IVC phase on development of porcine PA embryos
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Effect of FBS treatment period on development of porcine PA blastocysts
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Effect of hydrogen peroxide treatment during late IVC phase on early development of porcine PA embryos
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Effect of glutathione with hydrogen peroxide treatment during the early IVC phase on development of porcine PA embryos
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Effect of hydrogen peroxide with FBS treatment during late IVC phase on development of porcine PA embryos
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Effect of FBS and hydrogen peroxide treatment during early IVC phase on development of porcine PA embryos
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Effect of FBS treatment during late IVC phase on ICM and TE proportion and cellular survival of porcine SCNT blastocysts
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Effect of FBS and hydrogen peroxide treatment during early IVC phase on ICM and TE proportion and cellular survival of porcine PA blastocysts
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Effect of FBS supplementation timing on the development of porcine PA blastocysts.
(A and B) Representative photographs (A) and proportion (B) of the blastocysts at four different embryonic stages (EB, early blastocyst; MB, mid-blastocyst; ExB, expanded blastocyst; HB, hatched or hatching blastocyst).
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Effect of FBS treatment during late IVC phase on early development in porcine PA embryos
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Effect of FBS treatment during late IVC phase on ICM and TE proportion and cellular survival of porcine PA blastocysts
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Effect of FBS treatment during late IVC phase on ICM and TE proportion and cellular survival of porcine IVF blastocysts
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Effect of hydrogen peroxide with FBS treatment during late IVC phase on ICM and TE proportion and cellular survival of porcine PA blastocysts
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Effect of FBS treatment during late IVC phase on development of porcine SCNT embryos
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ROS generation and antioxidant gene expression during early development of porcine PA embryos.
(A) Fluorescence microscopy of 1-cell embryos to blastocysts treated with CM-H2DCFDA (left panel) and quantification of ROS levels (right panel) in the indicated groups. Bar = 100 μm. (B) qRT-PCR analysis of the relative abundance of Sod1, Prx II, Gpx1 an...
Effect of FBS and glutathione treatment during the early IVC phase on ICM and TE proportion and cellular survival of porcine PA blastocysts
(PDF)
Effect of ROS modulation during the early IVC phase on early development of porcine PA embryos.
(A and B) Representative photographs of blastocysts (white asterisks; A) developed in the presence or absence of 1.0 μM GSH and/or 0.1 mM hydrogen peroxide for 0–2 days of IVC and quantification of cleavage and blastocyst developmental rates in the indic...
Effect of FBS treatment during late IVC phase on development of porcine IVF embryos
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Effect of FBS with p38 MAPK and AKT inhibitors during the late IVC phase on development of porcine PA embryos
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Effect of FBS with p38 MAPK and p-AKT inhibitors during late IVC phase on ICM and TE proportion and cellular survival of porcine PA blastocysts
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Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonizati...
The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases i...
Although early diffusion lesion reversal after recanalization treatment of acute ischaemic stroke has been observed in clinical settings, the reversibility of lesions observed by diffusion-weighted imaging remains controversial. Here, we present consistent observations of sustained diffusion lesion reversal after transient middle cerebral artery oc...
Despite evidence of the presence of prostaglandin (PG) I2 in mammalian oviducts, its role in early development of in vitro-produced (IVP) embryos is largely unknown. Thus, in the present study we examined the effects of iloprost, a PGI2 analogue, on the in vitro developmental competence of early porcine embryos and the underlying mechanism(s). To e...
TSEN54 encodes a subunit of the tRNA-splicing endonuclease complex, which catalyzes the identification and cleavage of introns from precursor tRNAs. Previously, we identified an AluSx -derived alternative transcript in TSEN54 of cynomolgus monkey. Reverse transcription-polymerase chain reaction (RT-PCR) amplification and TSEN54 sequence analysis of...
Supplementary Figure S1. Verification of no genomic DNA contamination in various cDNA samples. Supplementary Figure S2. Putative translational sequence of alternative transcripts of TSEN54. Supplementary Figure S3. Multiple alignment analysis of human and primate MER54, AluYl17, and AluSx sequences in TSEN54. Supplementary Figure S4. PCR and multip...
ZKSCAN5 (also known as ZFP95) is a zinc-finger protein belonging to the Krűppel family. ZKSCAN5 contains a SCAN box and a KRAB A domain and is proposed to play a distinct role during spermatogenesis. In humans, alternatively spliced ZKSCAN5 transcripts with different 5'-untranslated regions (UTRs) have been identified. However, investigation of our...
BCS1L gene encodes mitochondrial protein and is a member of conserved AAA protein family. This gene is involved in the incorporation of Rieske FeS and Qcr10p into complex III of respiratory chain. In our previous study, AluYRa2-derived alternative transcript in rhesus monkey genome was identified. However, this transcript has not been reported in h...
In line with recent findings showing Alzheimer's disease (AD) as an insulin-resistant brain state, a non-transgenic animal model with intracerebroventricular streptozotocin (icv-STZ) administration has been proposed as a representative experimental model of AD. Although icv-STZ rodent models of AD have been increasingly researched, studies in non-h...
The accumulation and aggregation of misfolded proteins in the brain, such as amyloid-β (Aβ) and hyperphosphorylated tau, is a neuropathological hallmark of Alzheimer's disease (AD). Previously, we developed and validated a novel non-human primate model for sporadic AD (sAD) research using intracerebroventricular administration of streptozotocin (ic...
The coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) stress has been explored extensively, but little is known regarding their role in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and E...
Cellular fate of human neural stem cells (hNSCs) transplanted in the brain of nonhuman primates (NHPs) with no immunosuppression was determined at 22 and 24 months posttransplantation (PTx) regarding survival, differentiation, and tumorigenesis. Survival of hNSCs labeled with magnetic
nanoparticles was successfully detected around injection sites i...
We reported previously that the intracerebroventricular streptozotocin (icv-STZ)-treated cynomolgus monkey showed regionally specific glucose hypometabolism in FDG-PET imaging, similar to that observed in the early stages of sporadic Alzheimer's disease (sAD). However, further pathological analyses of this model at the molecular level are needed to...
While a critical role of autophagy in mammalian early embryogenesis has been demonstrated, few studies have been conducted regarding the role of autophagy in in vitro maturation (IVM) of immature oocytes. In the present study we investigated the effect of rapamycin, a chemical autophagy inducer, on the nuclear and cytoplasmic maturation of porcine...
In the investigation of the expression levels of target genes, reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is the most accurate and widely used method. However, a normalization step is a prerequisite to obtain accurate quantification results from RT-qPCR data. Therefore, many studies regarding the selection of r...
Nucleotide sequences of the candidate reference genes from the cynomolgus monkey.
(DOCX)
Despite the positive roles of histone deacetylase inhibitors in somatic cell nuclear transfer (SCNT), few studies have evaluated valproic acid (VPA) and its associated developmental events. Thus, the present study was conducted to elucidate the effect of VPA on the early development of bovine SCNT embryos and the underlying mechanisms of action. Th...
Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of sperm permeabilization efficiency...
Melting curve analyses of the candidate reference genes and target genes in the total samples.
(TIF)
Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) has been widely used to quantify relative gene expression because of the specificity, sensitivity, and accuracy of this technique. In order to obtain reliable gene expression data from RT-qPCR experiments, it is important to utilize optimal reference genes for the norm...
Nucleotide sequences of the candidate reference genes and target genes from the cynomolgus monkey brain.
(TIF)
Background
As a human replacement, the crab-eating macaque (Macaca fascicularis) is an invaluable non-human primate model for biomedical research, but the lack of genetic information on this primate has represented a significant obstacle for its broader use.
Results
Here, we sequenced the transcriptome of 16 tissues originated from two individuals...