Kamilla Vandsø Petersen's research while affiliated with Aarhus University and other places
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Publications (8)
Restriction endonucleases are expressed in all bacteria investigated so far and play an essential role for the bacterial defense against viral infections. Besides their important biological role, restriction endonucleases are of great use for different biotechnological purposes and are indispensable for many cloning and sequencing procedures. Metho...
With the increased effort for identification of anticancer compounds, there is a growing need for tools to investigate the activity of enzyme biomarkers. Human topoisomerase 1 is the only target of the camptothecin derivatives, and the cellular drug response depends on the enzyme activity. Here we use the colon cancer cell line Caco2 to investigate...
Background
Prediction of the pattern of organ involvement in patients with diffuse cutaneous systemic sclerosis (dcSSc) is supported by the specificity of autoantibodies. These autoantibodies are generally mutually exclusive and highly specific for dcSSc ¹ . The autoantibody reactivity allows patients to be stratified early in the disease course, l...
The murine cell line GRX has been introduced as an experimental tool to study aspects of hepatic stellate cell biology. It was established from livers of C3H/HeN mice that were infected with cercariae of Schistosoma mansoni. Although these cells display a myofibroblast phenotype, they can accumulate intracellular lipids and acquire a fat-storing li...
Background: Eukaryotic topoisomerase 1 is a potential target of anti-parasitic and anti-cancer drugs. Parasites require topoisomerase 1 activity for survival and, consequently, compounds that inhibit topoisomerase 1 activity may be of interest. All effective topoisomerase 1 drugs with anti-cancer activity act by inhibiting the ligation reaction of...
Citations
... The stem region contains a favored TOP1 cleavage site three bases upstream from the 3'-end amplification (RCA) mediated by the phi29 polymerase, which can perform RCA with strand displacement, yielding 10 3 tandem repeat products. During the RCA step, fluorescently labeled ( Figure 1F) or biotin-coupled ( Figure 1G) nucleotides can be incorporated 17 . Incorporation of fluorescently labeled nucleotides allows for detection of the RCPs either using a fluorescence microscope or a fluorescence scanner. ...
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... Unfortunately, generic tests for the systemic evaluation of viral contaminants are rather complex and undirected. Without precise knowledge of the virus authenticity, these detection methods are limited to electron microscopy and assays for retroviral reverse transcriptase [52,53]. In particular, the versatility of electron microscopy is an effective, universal, and unbiased means when an infectious viral agent is suspected [54]. ...
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