Kamil Cieslik's research while affiliated with Greenwater Laboratories and other places

Publications (2)

Article
Full-text available
Microcystins (MCs) and nodularins (NODs) exhibit high structural variability, including modifications of the Adda (3S-amino-9S-methoxy-2S,6,8S-trimethyl-10-phenyldeca-4E,6E-dienoic acid) moiety. Variations include 9-O-desmethylAdda (DMAdda) and 9-O-acetylDMAdda (ADMAdda) which, unless targeted, may go undetected. Therefore, reference standards were...
Article
In 2013 and 2015, the Pennsylvania Department of Environmental Protection conducted a survey of lotic habitats within the Susquehanna, Delaware, and Ohio River basins in Pennsylvania, USA, to screen for microcystins/nodularins (MCs/NODs) in algae communities and smallmouth bass (Micropterus dolomieu). Periphyton (68 from 41 sites), juvenile whole f...

Citations

... 019 were analyzed for exposure to microcystins and nodularins produced by cyanobacteria. Samples were first screened by FWRI using a validated ELISA. All positive results were subsequently analyzed for total Adda microcystins and nodularins (MMPB) by liquid chromatography and tandem mass spectrometry (LC-MS/MS) performed by GreenWater Laboratories (Foss et. al, 2020). Samples were cut (≤ 1 mm 3 ) and subsampled (ca 2-3 grams wet weight). Subsamples were transferred to homogenization vials with 10 mM phosphate buffer (pH=7) to achieve sample concentrations of 100 mg/mL. An Omni Bead Ruptor was used to homogenize the samples (6.3 m/s for 30s). Aliquots were dispensed into glass vials for extractions. ...
... So far, at least 279 MC variants have been reported . Cyanotoxins, especially MCs, are responsible for massive mortalities for small animals such as fish, birds, and turtles (Zimba et al. 2001;Nasri et al. 2008;Foss et al. 2017;Svirčev et al. 2019;Zhou et al. 2021). They have often been claimed as the suspicious cause of mass mortality of terrestrial mammals in southern Africa, including livestock (cattle and sheep) as well as nonwading wild mammals (white rhinos, blue wildebeest, giraffes, zebras, and impalas) . ...