Kabyl Zhambakin's research while affiliated with Institute of Plant Biology and Biotechnology and other places

Publications (15)

Article
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To cite this paper: Daurova A, D Daurov, K Zhapar, D Volkov, Z Sapakhova, M Shamekova and K Zhambakin (2022). Improvement of breeding-valuable traits of rapeseed (Brassica napus) using mutagenesis. Intl J Agric Biol Abstract This study evaluated the agronomic and qualitative characteristics and cold resistance of 46 doubled haploid mutant B. napus...
Article
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Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 show...
Article
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Potato (Solanum tuberosum L.) is the fourth most common food crop worldwide and one of the main food products in Kazakhstan. One of the factors leading to crop loss in potato is viral disease. There are more than 30 viruses known to cause diseases in potato. Therefore, using virus-free planting material is an important aspect of growing potato. It...
Article
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The article presents the results of a study on obtaining mutant doubled haploids of turnip rape (Brassica rapa L. ssp. oleifera). The culture medium for producing embryos in isolated microspore culture was optimized. The optimal medium formula was NLN + 13% S + 0.05 mg/L NAA + 0.05 mg/L BA. Embryos derived from isolated microspore culture were trea...
Article
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Biotechnological methods are becoming an integral part of biological research. This review presents some of the most significant scientific results of Kazakhstan biologists in the field of plant biotechnology over the past 10 years. One of the recent important areas of application of biotechnological methods is the conservation and study of plant g...
Article
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A screening of zoned in the Republic of Kazakhstan rice varieties for the amylose content has been carried out. The allele state identification of the waxy gene using a marker of Glu-23 showed that all the studied varieties contain a medium amount of amylose as compared to the standard Japanese varieties. A hybridization of the Bakanasskii and Akda...
Article
Full-text available
A screening of zoned in the Republic of Kazakhstan rice varieties for the amylose content has been carried out. The allele state identification of the waxy gene using a marker of Glu-23 showed that all the studied varieties contain a medium amount of amylose as compared to the standard Japanese varieties. A hybridization of the Bakanasskii and Akda...
Article
Full-text available
Kazakhstan has problem of soil wind erosion. Therefore, the use of zero agro techno logy is becoming very important. In order to obtain wheat lines resistant to glyphosate, chemical mutagenesis has been applied. Wheat seeds were treated with ethyl methanesulfonate (EMS) by three concentrations of 3 mM, 40 mM, 50 mM EMS and 0.05 M phosphate buffer f...
Article
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Interspecific hybrids of rapeseed (Brassica napus) with rape (Brassica campestris), and mustard (Brassica juncea) were obtained. Initial varieties have the appropriate amount of erucic acid and glucosinolates, high yield and seed quality. Forced hybridization with rapeseed and mustardwasperformed according reciprocal crosses scheme under controlled...
Article
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Although there is low precipitation in Kazakhstan, rice is grown in regions serviced by irrigation canals. And breeding has begun in order to reduce the cost of importing glutinous rice. To develop glutinous rice cultivars efficiently, we need information on the haplotypes of Wx and Alk genes that affect eating quality. We investigated the haplotyp...
Article
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The polymerase chain reaction (PCR) is fundamental to molecular biology and is the most important practical molecular technique for the research laboratory. The principle of this technique has been further used and applied in plenty of other simple or complex nucleic acid amplification technologies (NAAT). In parallel to laboratory " wet bench " ex...
Article
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The research was aimed at developing prebreeding resources of Kazakhstan rice varieties with colored pericarp for breeding. During the study, hybrid analysis of inheritance of the trait “colored pericarp” in breeding material used for the work was performed. Rice genotypes with colored pericarp, as well as white rice varieties possessing important...
Article
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Composition of seed storage proteins were evaluated in rice collection materials using electrophoresis in alkaline medium at presence of SDS. Optimal conditions for protein extraction and fractionation were determined, the classification of protein components spectrum and methods for composition protein formulation was developed. It was conducted t...

Citations

... DH technique has been successfully used in genetic mapping and wide hybridization studies in many important crops, specially wheat, rice, maize, barley, potato, citrus, oilseed rape, sunflower and apple (Maluszynski et al. 2003). For example, drought tolerant DH plants have been developed from two interspecific hybrids between Brassica napus and Brassica rapa (Daurova et al. 2020). The DH plants of wheat are obtained from wide hybridization (e.g. ...
... Thermotherapy (high or low temperature) and chemotherapy generally enhance the virus removal and affect the meristem regeneration frequency. Prolonged high temperature and chemicals, like ribavirin treatment, potentially decrease the meristem's regeneration frequency and growth and development of regenerated plantlets (Buko et al., 2020;Daurov et al., 2020;Zhang et al., 2019;Pramesh and Baranwal, 2015). The basic steps involved in meristem culture ( Fig. 1) are as follows: ...
... Recently, Kitashiba et al. (2016) have identified loci associated with embryo yield in microspore culture of Brassica rapa. Daurova et al. changed microspore-derived embryos (MDE) fatty acid composition (Daurova et al., 2020). Genes associated with the induction and the establishment of embryogenesis in isolated microspores were studied by Malik et al. (2007), and Tsuwamoto et al. (2007). ...
... In pest management, for instance, this method could be used in plant protection in virus test systems of plants, simplifying the requirements of plant quarantine agencies and increasing export potential (Chen et al., 2019;Gan & Xue, 2021). Kazakhstan has widely applied biotechnology and genetics to the grape industry (Zhambakin & Zhapar, 2020). ...
... In the end, it is not so much the total number of regenerants that is important for the breeder as the yield of spontaneously doubled haploids. Their share among different researchers varies from 25 to 95% [1,19]. The type of lighting influenced the value of this indicator in different directions (see Fig. 1). ...
... In developing FastPCR and online Java Web tools [13][14][15][16], our aim was to create a practical and easy-to-use software for routine manipulation and analysis of sequences for most PCR applications. The parameters adopted are based on our experimental data for efficient PCR and are translated into algorithms to design combinations of primer pairs for optimal amplification. ...
... The proportion of Fb1 chains (DP = 13-24) was significantly lower in SN625 than in TN013; the proportion of amylopectin Fb1 chains (DP = 13-24) in many CSSLs also increased compared with parent SN625 (Figure 2b). The proportion of amylopectin Fb2 chains (DP = [25][26][27][28][29][30][31][32][33][34][35][36] was also lower in SN625 than in TN013. Moreover, the proportion of Fb2 chains in many CSSLs was higher than in SN625. ...
... In Japan, improved pigmented rice variety was developed by crossing black rice variety 'Okunomurasaki' with high quality-white rice variety 'Koshihikari' [137]. In Kazakhstan, adapted pigmented rice variety was developed by crossing pigmented and non-pigmented rice varieties [138]. In Thailand, a new deep purple rice variety 'Riceberry' was developed by crossing between non-glutinous purple rice and an aromatic white jasmine rice variety [139][140]. ...