September 2000
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14 Reads
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13 Citations
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... Later it was also shown that HIV particles prefer to bind at the edges of cholesterol-rich lipid domains that were reconstituted in supported bilayers [86,87]; however, observing this preference in live cells due to the small scale and dynamism of lipid rafts is difficult, illustrating the power of using an in vitro system for such studies. Focusing further on the receptors themselves, TIRF microscopy has also been used to measure the affinity of HIV glycoprotein 120 for the glycosphingolipids galactosyl ceramide, glucosylceramide, lactosylceramide and α-hydroxy glucosylceramide in SLBs [88,89]. The affinity of glycoprotein 120 for these lipids is roughly 5 times lower than its affinity for CD4 [90]. ...
September 2000