K R Vadalia’s scientific contributions

Helicobacter pylori (H. pylori) infect more than half of the world population, making it one of the most prevalent infections. H. pylori is now accepted as the most common cause of histologic gastritis and is responsible for the majority of cases of peptic ulcer disease and gastric cancer. Approximately 1 in 6 (17%) persons with H. pylori infection will develop peptic ulcer disease, and each year 1% to 2% of these will experience a major or life-threatening complication, and this basically occurs due to short gastric residence time of antimicrobial agents, keeping that in mind mucoadhesive microspheres were prepared to increase gastric residence time using solvent evaporation method. The (mucoadhesive) sustained release of amoxicillin trihydrate is desired because of its short biological half-life. Predominantly to treat H. pylori infections, the mucoadhesive is desired to be confined to the upper gastrointestinal tract. Amoxicillin trihydrate mucoadhesive microspheres were prepared using Eudragit RS100 as matrix and HPMC K4M as mucoadhesive polymer for the potential use of treating gastric and duodenal ulcers, which were associated with H.pylori. The morphological characteristics of the mucoadhesive microspheres were studied under scanning electron microscope. The percentage yield of microspheres of all formulation was in the range of 78.90% to 90.95%. The drug content determination showed that even if the polymer composition was changed the solvent evaporation process was highly efficient to give microspheres having maximum drug loading. In termination, the prolonged gastrointestinal residence time and enhanced amoxicillin trihydrate stability resulting from the mucoadhesive microspheres of amoxicillin trihydrate might make contribution to H. pylori clearance.

Helicobacter pylori (H. pylori) infect more than half of the world population, making it one of the most prevalent infections. H. pylori is now accepted as the most common cause of histologic gastritis and is responsible for the majority of cases of peptic ulcer disease and gastric cancer. Approximately 1 in 6 (17%) persons with H. pylori infection will develop peptic ulcer disease, and each year 1% to 2% of these will experience a major or life-threatening complication, and this basically occurs due to short gastric residence time of antimicrobial agents, keeping that in mind mucoadhesive microspheres were prepared to increase gastric residence time using solvent evaporation method. The (mucoadhesive) sustained release of amoxicillin trihydrate is desired because of its short biological half-life. Predominantly to treat H. pylori infections, the mucoadhesive is desired to be confined to the upper gastrointestinal tract. Amoxicillin trihydrate mucoadhesive microspheres were prepared using Eudragit RS100 as matrix and HPMC K4M as mucoadhesive polymer for the potential use of treating gastric and duodenal ulcers, which were associated with H.pylori. The morphological characteristics of the mucoadhesive microspheres were studied under scanning electron microscope. The percentage yield of microspheres of all formulation was in the range of 78.90% to 90.95%. The drug content determination showed that even if the polymer composition was changed the solvent evaporation process was highly efficient to give microspheres having maximum drug loading. In termination, the prolonged gastrointestinal residence time and enhanced amoxicillin trihydrate stability resulting from the mucoadhesive microspheres of amoxicillin trihydrate might make contribution to H. pylori clearance.

Cassia auriculata is a tropical tree locally belonging to the family Caesalpiniaceae found widely in India. Seeds of plant contain gluco-mannose hence attempt to evaluate the seeds for suitability as tablet binder is considered and the present investigation reports the isolation of mucilage of Cassia auriculata seed. The DSC and FTIR thermograms of drug and gum indicated no chemical interaction. Phytochemical characteristics of mucilage, such as carbohydrate, protein and flavanoids etc. were studied. Physiochemical characteristics of mucilage, such as solubility, swelling index, loss on drying, viscosity, hydration capacity, powder porosity, microbiological properties and pH were studied. The mucilage was evaluated for its granulating and binding properties in compressed tablet, using Diclofenac sodium as model drug. Mucilage was used in four different concentration i.e. 0.25, 0.5, 0.75 and 1.0% w/v. The granules were prepared by wet granulation process. The prepared granules were evaluated for percentage of fines, average particle size, compressibility index and flow properties. The properties were compared with Guar gum, which was used as standard binder at 1.00% w/v concentration. The tablet were prepared and evaluated for content uniformity, hardness, friability, disintegration time and in vitro dissolution profile. The tablets had good physiochemical properties, and the drug release was more than 85% within 3 hour. It was observed that increasing the concentration of mucilage increases hardness and decreases the disintegration time. All the formulations (F1, F2, F3, F4 and F5) were subjected to stability studies for three months at 25° C/60% RH, 30° C/65% RH and 40° C/75% RH as per ICH guidelines, only F1 and F2 showed stability with respect to release pattern and other parameters which confirm the use of mucilage as excipient.

Hemidesmus indicus R.Br. is a treasure of the forest and herbal wealth. It is being used as folk medicines and as an ingredient in Ayurveda and Unani preparations against diseases of blood, inflammation, diarrhea, urinary disorders and rheumatism etc. The objective of the present study was to evaluate the role of natural nephroprotectant Hemidesmus indicus for the treatment of congestive heart failure. Hemidesmus indicus extracts were evaluated by using salt water induced left ventricular hypertrophy in Spargue dawley rat model. The property was evaluated using serum creatinine, urea, SGOT, SGPT and urine albumin, creatinine, Na+, K+ and Ca++ as biochemical parameters and histopathological changes including myocyte diameter, neutrophil infiltration and cardiac hypertrophy. Results obtained from the present study indicates that aqueous and methanolic extracts have more significant inhibitory effect on salt water feeding induced severity of microalbuminuria, serum urea and creatinine, myocyte diameter and retention of Na+ and water and increases the serum calcium level. We conclude from the present study that, potent natural nephroprotectant Hemidesmus indicus is also a potent cardioprotective.