Jyotirmaya Mohanty's research while affiliated with Central Institute of Freshwater Aquaculture and other places

... βGRPs have been identified in several invertebrate species, their function is the recognition of carbohydrate structures in pathogens such as bacteria, fungi or protozoa; subsequently, they activate several mechanisms, including the proPO system, phagocytosis, and respiratory burst (Chai et al., 2018;Sahoo et al., 2023). The nomenclature of the βGRP family members is based on their different recognition capacities; the family includes lipopolysaccharide (LPS) and β-1,3-glucan binding proteins (LGBPs), β-1,3-glucan binding proteins (βGBPs), and gram-negative bacteria binding proteins (GNBPs) (Chai et al., 2018). ...

... Therefore, top priority needed to be given for management of this disease to save the aquaculture industry from the vast loss. Several works have been carried out to prevent argulosis (Rahman, 1968;Chandra et al., 2004;Bakshi et al., 2006;Sahoo et al., 2021 ). For the treatment of this disease, like other ectoparasitic diseases, fish farmers apply various chemical substances such as formalin, hydrogen peroxide, dichlorvos, cypermethrin, levamisole, diflubenzuron, Chloramine-T, deltamethrin, azamethiphos, ivermectin (Powell and Speare, 1996;McAndrew et al., 1998;Pike and Wadswath, 1999;Toovey and Lyndon, 2000;Hemaprasanth et al., 2012). ...

... Immunoproteomic emerged as popular techniques that help in the identification of suitable immunoreactive antigens. Using 2D electrophoresis and western blot technique, 14 immunogenic spots were identified from A. siamensis from which bromodomain-containing protein, anaphase-promoting complex subunit 5 and elongation factor-2 were suggested as a suitable vaccine candidates (Das et al. 2021). A western blot system has been developed using immunized fish, rohu serum and other reagents to analyse the immunoreactive proteins in A. siamensis antigens (Das et al. 2018a). ...

... Among different parasitic diseases, infection with Dactylogurus is one of the major threats noticed in freshwater fish farming sector worldwide (Paul et al., 2021). The report on the prevalence of this particular parasite in aquaculture systems of India is scarce. ...

... The overall health of shrimps and prawns in semi-intensive cultures and farming practices is compelling as it caters to the overall needs of marine shellfish-processing industries for export and revenue generation. In prawns and shrimps, lectins are regarded as bi-functional molecules involved in the sensing of pathogenic infections, where they possess potent antibacterial, antifungal, and antiviral properties, orchestrating an indispensable role in innate immunity (Sun et al. 2008;Wang et al. 2014;Mohanty et al. 2020). CTLs and LTL domain lectins have been at the forefront among the lectin family members contributing to host immune surveillance, thus serving as a biotherapeutant for shrimps or prawns (Wei et al. 2012;Wang et al. 2014;Huang et al. 2019). ...

... Zhu et al. [15] have reported 20 lectin transcripts in M. rosenbergii including C-type lectin, L-type lectin and lectin with low-density lipoprotein class A. Other types of lectins such as mannosebinding lectin and L-type lectin have also been studied in this species [16,17]. Besides, several diverse proteins possessing lectinlike properties have also been reported in this species, e.g., hemocyanin [18] and LGBP [19]. However, more information is necessary to further decipher the role of lectins in the immune mechanisms of this prawn and its possible modulation in protecting the species against microbial invasion. ...

... Ganapathy, unpublished results). Quantitative real-time polymerase chain reaction (qRT-PCR) is the most effective method for gene expression analysis, and normalization using suitable reference genes is essential in obtaining reliable gene expression results (Jose et al. 2020;Li et al. 2017;Sahoo et al. 2019;Yuan et al. 2014). The selection of internal control genes is based on the consistency of expression across all tissue samples and under different growth conditions. ...

... Rao et al. [14] have found significant differential expressions in immunerelated genes from the hepatopancreas of M. rosenbergii infected with Vibrio parahaemolyticus. A number of lectin transcripts have also been reported in the hepatopancreas of this species using transcriptome-wide analysis [15,16]. ...

... It consists of 190 amino acids, with a calculated molecular mass of 20.87 kDa and a theoretical isoelectric point (pI) of 5.96. Most fish LysG have a similar molecular mass, like sea bass (Dicentrarchus labrax L.), grass carp (Ctenopharyngodon idellus), and rohu (Labeo rohita), ranging from 20.19 to 20.27 kDa [19,20,57]. The TrLysG was found to lack a signal peptide, suggesting its potential as an intracellular protein primarily engaged in defense mechanisms following pathogen invasion of the host cell [58]. ...

... Indirect methods were used to obtain NO level in the serum by estimating nitrite/nitrate using Griess reaction as per the methods described by Das et al. (2018). To estimate nitrite level in the serum, about 100 µl of serum was diluted with 400 µl of carbonate buffer. ...