Jyotirmaya Mohanty's research while affiliated with Central Institute of Freshwater Aquaculture and other places

Publications (53)

Article
Full-text available
Lectin protein families are diverse and multi-functional in crustaceans. The carbohydrate-binding domains (CRDs) of lectins recognize the molecular patterns associated with pathogens and orchestrate important roles in crustacean defense. In this study, two lectin homologs, a single CRD containing C-type lectin (CTL) and an L-type lectin (LTL) domai...
Article
Aim An immunoproteomic approach was followed to identify immunoreactive antigens of fish ectoparasite, Argulus siamensis with rohu (Labeo rohita) immune sera for screening of potential vaccine candidates. Materials and results The whole adult Argulus antigen was run in 2D electrophoresis with IEF in 7 cm IPG strips of pH 4‐7 and SDS‐PAGE with 12%...
Article
Dactylogyrus is one of the major parasites ubiquitously present in the aquatic environment causing severe economic loss. The present study documented a new species of Dactylogyrus from India infecting the Indian major carp, i.e. rohu (Labeo rohita) both in natural and experimental conditions. The 18 s and 28 s rDNA sequence analyses revealed the pr...
Article
Aim: Identification of full-length ORF of hemocyanin subunit-1 (Mr_HC_1) from the hepatopancreas transcriptome of freshwater prawn, Macrobrachium rosenbergii infected with Vibrio harveyi and characterization of its sequence and structure by in silico tools and softwares. Methodology: Illumina HiSeq and de novo assembled unigenes were scanned agains...
Article
The giant freshwater prawn, Macrobrachium rosenbergii, is mass cultivated with production exceeding 200,000 tons per year since 2002. Lately, bacterial and viral infections have resulted in mortalities significantly affecting production. Vibrio harveyi causes hepatopancreatic nuclear deformation and lipid breakdown in post‐larval juvenile prawns. T...
Article
Full-text available
Lectins are proteins that bind to the carbohydrate moieties on surface of bacteria, erythrocytes and other cells of invertebrates causing agglutination and mediate in recognition of foreign substances. In the present study, we isolated and characterized a lectin molecule present in the hemolymph of Macrobrachium rosenbergii, an important cultured f...
Chapter
Full-text available
Macrobrachium rosenbergii is one of the widely cultured freshwater prawn species. The farmed output has expanded due to high priority as food and food products for consumption and export. The expansion in the culture of species to alien habitats such as rice fields, orchard gardens, river banks etc., high stocking density and intensive farming has...
Chapter
Shrimp aquaculture has become a global industry with over 85% of worldwide farmed shrimp produced in Asia. RNA interference (RNAi) is a useful approach to identify fitness traits associated with immunity and defense of shrimp infected with microorganisms. In this approach, an in vivo gene-silenced model is constructed by administering sequence-spec...
Article
Full-text available
Argulus spp. are economically important fish ectoparasites. The development of antiparasitic drugs is thus important and real time PCR is an indispensable tool in drug development. The analytical potential of RT-PCR depends upon accurate normalisation by the use of stable reference genes. Here, we identified stable reference genes of Argulus siamen...
Article
Lysozyme, an important secretory innate immune component, possesses antimicrobial activity against broad spectrum of bacteria and viruses. In the present study, complete CDs (558 bps) of g-type lysozyme of rohu (Labeo rohita) was amplified and translated for a putative protein of 185 amino acids. The domain architecture and tertiary structure was a...
Article
Full-text available
A study was undertaken to develop a western blot method for detection of immunogenic proteins of fish ectoparasite, Argulus siamensis for its further use as potential vaccine candidates. Argulus antigens were prepared by homogenization and injected to rohu (Labeo rohita) juveniles for development of immune serum. The serum was used to immunostain t...
Article
The knowledge of mucosa-associated molecular events that occur during infections is scarce despite the well-established importance of mucus in fish immunity. Using qRT-PCR, we analyzed the immune gene expression patterns in mucus of Labeo rohita experimentally infected with an ectoparasite Argulus siamensis. Mucus samples were collected at 0 h, 12...
Article
Parasite species often show a heterogeneous, highly dispersed pattern of infestation within hosts. Varieties of factors including morphological, physiological, immunological and nutritional characteristics affect the infestation level of a specific parasite in homogenous pray. Limited attempts, however, have been made to explore such underlying dri...
Article
Full-text available
Argulus spp. are important ectoparasites of fish, and the current approach of their control using chemical pesticides has numerous drawbacks. Vaccination is a promising alternative but identification of protective antigens is a limiting step. The ribosomal protein P0, essential for protein synthesis, has been studied as a vaccine candidate. We gene...
Patent
This a patent for obtaining year round gonad maturity and spawning in Indian major carps and other carp applying particular photo-thermal regime
Article
Branchiuran ectoparasites of the genus Argulus can have extensive damaging effects on cultured fish. There exist no systematic studies that evaluate susceptibility or resistance of various carp species to Argulus sp. and the underlying mechanisms. The present study aimed at identifying the most susceptible and resistant cultured species, studying s...
Article
Full-text available
Abstract: Giant freshwater prawn, Macrobrachium rosenbergii is an important freshwater crustacean widely cultured in several countries and is the most cultured freshwater prawn species in India. Of late, its production has come down due to slow growth rate and disease occurrences. The Central Institute Freshwater Aquaculture (CIFA), Bhubaneswar in...
Article
β-glucan binding protein (βGBP), a pattern recognition protein was purified from the haemolymph of freshwater prawn Macrobrachium rosenbergii by heparin affinity chromatography that showed a single band in native gradient PAGE. The β-glucan binding property of the purified protein was confirmed in a phenoloxidase (PO) assay, where addition of βGBP...
Article
In the present article, immunoglobulin (Ig) of Puntius sarana (a vulnerable medium carp species) was purified by affinity chromatography, characterized, and identified as only IgM type with a native molecular weight of 879 kDa having one heavy (88 kDa) and one light (26 kDa) chain. Further, the developed rabbit antisera against IgM was found to be...
Article
Vitellogenin was purified from the serum of 17‐β estradiol (E2)‐induced juvenile Catla catla using a simple two‐step purification procedure i.e. selective chemical precipitation followed by gel filtration chromatography. Purified protein migrated as single band in a native gradient PAGE which indicated the purity of the sample. The molecular weight...
Article
Argulus siamensis is the most damaging fish parasite prevalent in the freshwater aquaculture systems of India. In an attempt to further understand the behavior of this economically important parasite, the means of biological transmission, egg laying strategies and effect of temperature on development of eggs was studied. A. siamensis showed opportu...
Article
Argulus siamensis is a major ectoparasitic pathogen of freshwater fish capable of causing substantial economic loss. None of the available control measures have been able to address the problem of argulosis resourcefully. To combat this pathogen effectively, it is necessary to have a comprehensive understanding of its life processes with informatio...
Article
Argulus siamensis is a major pathogen in freshwater aquaculture. The immune responses of Indian major carp, Labeo rohita to experimental infection of A. siamensis was evaluated by quantitation of immune-relevant gene expression in head kidney and skin, and serum innate immune parameters through the course of infection. In skin of infected fish, ant...
Article
The immunoglobulin (Ig) from the serum of Channa striata was isolated by gel electroelution and characterized further to understand its nature and subsequent applications in studying the immune response. The purity of the sample was confirmed with the presence of a single band on native gradient PAGE and the molecular weight of ∼897 kDa was determi...
Article
A study on vitellogenin (Vtg), the egg yolk precursor protein from Asian catfish, Clarias batrachus was undertaken. Vtg synthesis was induced in male C. batrachus by three intraperitoneal injections of 17β-estradiol (E2). It was purified from E2-induced plasma by precipitation with EDTA and MgCl2 followed by gel filtration chromatography on Sephacr...
Article
Argulosis caused by crustacean ectoparasites of the genus Argulus is one of the parasitic diseases that pose a major threat to freshwater aquaculture industry around the globe. The actual loss incurred due to this disease has gone mostly unnoticed. In the present study, economic burden imposed by Argulus sp. in carp culture ponds in India was estim...
Article
Labeo rohita (rohu) fingerlings were exposed to 0.002, 0.004 and 0.02ppm of Furadan, a carbamate pesticide for 96h. Samplings were done at 24, 48, 72 and 96h. The study was intended to assess tissue specific genotoxic effects produced by furadan on two different tissue systems in fish. Results showed low baseline DNA break in blood cells followed b...
Article
Argulus is one of the most important fish parasites that cause heavy economic loss to aquaculture industry. The present investigation was undertaken to study the genetic diversity of the Argulus sp. collected from 13 locations representing major aquaculture zones in India by RAPD analysis and to develop species‐specific markers. Thirteen random dec...
Article
The genus Argulus is economically one of the most important taxa of crustacean ectoparasites in freshwater aquaculture systems throughout the world. This study investigates the occurrence and effect of a mixed infestation of two species of Argulus, Argulus japonicus Thiele, 1900 and Argulus siamensis Wilson, 1926, in a carp culture farm in Mandi, H...
Article
The efficacy of two avermectins, doramectin and ivermectin via oral and parenteral routes and their prophylactic effects against Argulus siamensis infestation in Indian major carp, Labeo rohita was studied. Doramectin and ivermectin were fed once to L. rohita sub-adults naturally infested with A. siamensis parasites at graded levels of 1000, 750 an...
Article
Full-text available
Successful development of immunoprophylaxis against freshwater fish lice Argulus siamensis (Wilson) depends on the proper identification of protective antigens. The present study was undertaken to determine the immunodominant polypeptides of the freshwater fish lice A. siamensis, which causes a major disease problem in freshwater aquaculture. Sever...
Article
Labeo rohita (rohu) fingerlings were exposed to different concentrations (0.001, 0.002 and 0.01 ppm) of phorate, an organophosphate pesticide; samplings were done at 24, 48, 72 and 96 h. The study was carried out to evaluate tissue specific genotoxic effects produced by phorate, on three different tissue systems and to assess DNA repair response in...
Article
Full-text available
We report here for the first time significant advancement of sexual maturation and off-season breeding of Indian major carp (IMC) species, rohu (Labeo rohita), catla (Catla catla) and mrigal (Cirrihinus mrigala) through photothermal manipulation during winter at least 4-5 months prior to their normal spawning season (June-August). The spent fishes...
Article
The study was undertaken to determine the modulation in innate immune response of rohu (Labeo rohita) during experimental freshwater lice Argulus siamensis infection. Results showed that serum α-2 macroglobulin (α-2M) activity, ceruloplasmin level and alternative complement activity were significantly (P<0.05) lower in fish at different degrees of...
Article
The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control an...
Article
Lectins play important role in innate immunity of animals. The affinity of the natural haemagglutinin of the giant freshwater prawn Macrobrachium rosenbergii towards vertebrate erythrocytes and its level with relation to sex, size and moult stages were studied. The strongest agglutinating titres in haemolymph of prawns were marked against guinea pi...
Article
Live non-opsonized and opsonized Aeromonas hydrophila were injected into juveniles of freshwater prawn Macrobrachium rosenbergii to study the cells involved in phagocytosis, distribution of bacteria, cellular reactions and clearance of both forms of bacteria from the system. The bacteria were rapidly distributed to various tissues viz., gills, hear...
Article
An attempt has been made for multiple spawning of Asian catfish, Clarias batrachus, by sustained hormone administrations through injection and pellet at three different levels of human chorionic gonado-tropin (HCG): 150, 200, and 250 IU. The size of the eggs and biometry of larvae were significantly higher (P< 0.05) in hormone-treated females compa...
Article
A preliminary study on humoral defence factors of Indian river prawn, Macrobrachium malcolmsonii was carried out. The serum of animals collected from intermoult stage had an average total protein content of 9.65 g dl(-1) and lysozyme-like activity of 0.04 unit ml(-1). The phenoloxidase activity in haemocyte lysate supernatant was triggered signific...
Article
A new, safe, and rapid technique for the individual separation of the embryos of giant freshwater prawn Macrobrachium rosenbergii de Man is described. Two protease enzymes, e.g., trypsin and collagenase were used. Embryos in the advanced stage of development (gray embryos with eyespot and heart beat) were selected for the study. Treatment with coll...

Citations

... The ICAR-CIFA has interdisciplinary research collaborations, proper amenities for wet lab maintenance, skilled manpower, basic instruments, and molecular biology laboratory facility with widely experienced researchers to address the challenges faced in fish louse Argulus management. Currently, the group is actively involved in screening as well as delivery of vaccine targets by a combination of genomics and immunoproteomics [35] approach through exploration of mucosal immunity in fish. ...
... In order to gain insights into the transcriptome diversity of L. crocea tissues, transcriptome association analysis of multiple tissues still need to be addressed. Fish skin and gills are organs that are directly exposed to external environment, play an important role in protecting the body from pathogen infection [23,24]. They are also the primary targeting organ and major parasitic site of the C. irritans infection, which causes fish hypoxia and bacterial secondary inflammation, and even death [2,23]. ...
... The overall health of shrimps and prawns in semi-intensive cultures and farming practices is compelling as it caters to the overall needs of marine shellfish-processing industries for export and revenue generation. In prawns and shrimps, lectins are regarded as bi-functional molecules involved in the sensing of pathogenic infections, where they possess potent antibacterial, antifungal, and antiviral properties, orchestrating an indispensable role in innate immunity (Sun et al. 2008;Wang et al. 2014;Mohanty et al. 2020). CTLs and LTL domain lectins have been at the forefront among the lectin family members contributing to host immune surveillance, thus serving as a biotherapeutant for shrimps or prawns (Wei et al. 2012;Wang et al. 2014;Huang et al. 2019). ...
... It has been noticed that the ectoparasite develop resistance to most of the drugs used, thereby requiring frequent application of medications during a single culture operation [13], thus creating a potential for resistance development [14]. For example, frequent treatment with emamectin benzoate (SLICE™) against control of A. coregoni leading to tolerance development [14], insecticide lindane resistance by this species of parasite [13] and our own observations from field level applications of wide array of drugs by carp farmers with poor effectiveness prove the tolerance or resistance development towards available parasiticides by this group of parasites [15]. Similar resistance pattern was also noticed in sea louse to azamethiphos and teflubenzuron [16], and in terrestrial animals to wide array of drugs [17]. ...
... MDA is a product of polyunsaturated fatty acid peroxidation that directly reflects the level of lipid peroxidation [36]. LZM may contribute positively to fish resistance against pathogen challenge [37]. Previous studies in fish fed BSFLM-based diets have not found negative effects on the above enzymes, and have even reported positive effects on some enzymes. ...
... Indirect methods were used to obtain NO level in the serum by estimating nitrite/nitrate using Griess reaction as per the methods described by Das et al. (2018). To estimate nitrite level in the serum, about 100 µl of serum was diluted with 400 µl of carbonate buffer. ...
... Over the years, ICAR-CIFA has developed a challenge facility for year-round availability of Argulus parasite and standardized challenge protocol. In another attempt, a western blot method has also been developed and used to identify immunogenic proteins of Argulus that could be targeted as potential vaccine candidates [34]. The ICAR-CIFA has interdisciplinary research collaborations, proper amenities for wet lab maintenance, skilled manpower, basic instruments, and molecular biology laboratory facility with widely experienced researchers to address the challenges faced in fish louse Argulus management. ...
... Moreover, these parasites suck very little amount of blood from the host, and the vaccine candidate should be strong enough to protect, even with little blood meal by the parasite. Again unlike mammalian secretory antibody IgA, the counterpart secretory antibody molecules like IgZ or IgD in fish mucus or skin needs further in-depth investigation [22,23] and currently, there is no detection system available to measure the antibody levels in the skin or mucus using antibody-based assay system. Though oral vaccination would be relatively easy to administer by mixing in the feed as well as cost-effective from handler's and fish health perspective; but mass oral vaccination of fish is not routinely practiced. ...
... Hence, the parasitic load on fish would have been influenced by the host size, local inflammatory reaction and immunity . (Parida et al., 2018). Before the start of treatment mortality of 10-40% was observed over a period of one week with typical clinical signs of parasitic infestation. ...
... A recent report showed promising effects of fish vaccination with recombinant proteins against adult female (P33) or chalimus II (P30) sea lice infestations [28]. In a similar manner, we had performed an initial trial of ribosomal P0 peptide as a potential vaccine candidate for control of fish ectoparasite infections, i.e., A. siamensis in L. rohita and noted delayed mortality with relative percent survival of 16% following experimental challenge [29]. The use of RNA interference (RNAi) presents yet another valuable tool for screening of potential targets and development of vaccines against arthropod ectoparasites [30]. ...