Jurgen Kreutzberger's scientific contributions

Publications (2)

FIG. 1. Spotting pattern of Arabidopsis protein microarrays. Two...
FIG. 2. Immunoscreening (a and b) and phosphorylation studies (c– e) on...
FIG. 3. Duplicate correlation of signals of spotted proteins...
Article
Full-text available
Mitogen-activated protein kinase (MAPK) cascades are universal and highly conserved signal transduction mod- ules in eucaryotes, including plants. These protein phos- phorylation cascades link extracellular stimuli to a wide range of cellular responses. However, the underlying mechanisms are so far unknown as information about phosphorylation subst...
Fig. (1). Detecting targets of the small molecule biotin on...
Article
Full-text available
Microarray technology plays an increasing role in proteomic research. We give an overview about recent developments in this technology focusing on molecular interaction studies using protein and antibody microarrays. We report about technical aspects in the development of protein microarrays and describe different surfaces and detection modes. Furt...

Citations

... The various strategies used include yeast-two hybrid screens, protein microarrays and in vitro kinase assays. Microarray-based testing of 1690 Arabidopsis proteins identified 48 in vitro substrates for MPK3 and 39 for MPK6, with an overlapping set of 26 candidates (Feilner et al., 2005). High-density protein microarrays used to determine the phosphorylation targets of 10 different MAPKs identified 570 potential MAPK targets out of 2158 candidates (Popescu et al., 2009). ...
... Conventional detection methods for microarrays often use fluorescent dyes, which were detected by irradiation from a laser scanner. Detection methods are discussed intensively elsewhere (Espina et al. 2004;Feilner et al. 2004), while in this study, only new approaches should be pointed out to reach much higher sensitivities. With a special method called MIST (multiple spotting technique) (Angenendt et al. 2003a, b), multiple spotting steps are performed successively to one position. ...