Jürgen Kreutzberger's research while affiliated with Robert Koch Institut and other places

... Applying antigen microarray technology [17] we describe here the array manufacture process and subsequent analysis procedures leading to the identification of disease specific AAbs through profiling of preoperative sera derived from patients with resectable PDACs, CP, both AIP types, other GI-tract diseases and healthy controls. ...

... Using this technique to investigate A. thaliana transcription factor (TF) interactions, a deep-coverage A. thaliana TF interaction network was created (AtTFIN-1), greatly expanding the number of known plant TF interactions ( Trigg et al., 2017). Other systematic approaches include classic in vitro protein arrays (Feilner et al., 2005;Popescu et al., 2007Popescu et al., , 2009 • Interactome studies mentioning hubs are marked with a gray background. ...

... Hence, insights gained from studies of OMP folding and biogenesis are also vital for our understanding of human physiology (53) and will be key in guiding our choice of targets for the generation of new antibiotics and vaccines against Gram-negative bacteria (54). Consequently, a number of academic groups and drug companies have ongoing research projects targeting the essential OMPs BamA (the central β-barrel-containing subunit of BAM) and LptD (50, 52, [55][56][57][58][59][60][61][62], with at least six reports of inhibitors of their function in 2018-2019 alone (63)(64)(65)(66)(67)(68). ...

... MPER-TM proteoliposomes [136] His-tagged MPER bound to liposomes [137] Trimeric MPER fused to the diphtheria toxin domainA [138] A peptide containing 4 copies of the 10E8 epitope [139] Three different 6-helical bundle gp41 constructs containing different bundle destabilizing mutations [140] MPER peptide associated with liposomes [141] Chimeric human Rhinoviruses expressing MPER [142] The MPER and gp41 ectodomain was expressed separately as N-terminal fusions to the E2 protein of Geobacillus stearothermophilus; Immunization in conjunction with DNA encoding full-length SF162 gp160 [143] Hybrid antigens containing the MPER and the FPPR of gp41 of HIV-1 and sequences of the TM protein p15E of PERV [144] Tri-repeat of the MPER epitope of gp41 plus defending containing proteoliposomes [145] Fusion intermediate conformation of gp41 covalently linked to liposomes [83] Gp41 HR2-MPER-TM proteoliposomes [78] Bovine papillomavirus VLPs with extended 2F5 or 4E10 epitopes or the MPER domain grafted into the D-E loop of BPV L1 [146] A HEK293 cell line expressing membrane-anchored gp41 [147] Gp41-subunit antigens grafted onto liposomes/ virosomes [148] Gp140 oligomer prime followed by MPER peptide-liposome boost [149] Trimeric MPER fusion proteins [150] Gp41 ectodomain fused to an influenza HA2 region [151] ...

... Therefore, protein microarrays were also employed as a tool for phosphoproteomic analysis, as this allows detection of phosphorylation targets as well as molecular interactions using thousands of immobilized probable protein targets [32][33][34][35]. This technique was first employed for A. thaliana proteins [36]. Before designing the high-throughput microarrays with A. thaliana kinases [37], low throughput microarrays were designed to understand the number of microarrays analyzed for one phosphorylation on one microarray [38,39] followed by medium throughput with barley kinases [40]. ...

... The first important advancement is the use of an antibody-immobilized format, which in this context has been proposed in [19], in contrast to antigen-immobilized formats, which are recommended in most textbooks and articles. The second improvement is the miniaturization of the assay, which is achieved by the use of a microarray format, which has been used favorably in many applications, e.g., [20][21][22][23][24]. This enables the fast and easy performance of screening, sometimes with only a single chip. ...

... Most existing techniques used for identifying specific substrates are enzyme-specific, labor-intensive, and often not straightforward. Such approaches include the use of genetic and pharmacologic perturbations 11 , substrate-trapping mutants 12 , affinity purification-mass spectrometry 13 , utilizing peptide 14 or protein arrays 15 , tagging the client proteins by substrate analogs using engineered enzymes 16 and peptide immunoprecipitation 17 or the employment of sophisticated computational tools 18 . Most of these techniques are specifically designed for a certain enzyme or enzyme class, which limits their applicability. ...

... Protein microarrays are a high-throughput technology that can measure protein interactions and associated functions, with potential uses in cancer biomarker discovery [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. We have previously developed a custom cancer-specific protein array which measures antigenspecific antibodies present in patient blood [15,16]. ...

... Nowadays, a faster and more powerful technology to perform epitope mapping is represented by protein microarrays, which allow analysing simultaneously short and long peptides that are representative of all immunogenic regions of an antigen, including both linear and conformational epitopes, with the further advantage of using only minimal volumes of biological samples. Proteomic microarrays generated by spotting full-length antigens are largely used to profile responses to bacterial infections 25,26 or following vaccination [27][28][29] or as diagnostic tool 30 . ...

... Antibody microarray is an affinity based proteomic approach that uses miniaturized analytical systems generated by spatially arraying small volumes (nanoliter scale or less) of individual antibodies at discrete positions on a solid support ( Fig. 1) , Kusnezow et al., 2006a, Angenendt, 2005, Pavlickova et al., 2004, Glokler and Angenendt, 2003. The number of antibodies used in an assay has varied from a few to several hundred, and was rarely larger than one thousand. ...