Jude W. Grosser's research while affiliated with University of Florida and other places

Publications (260)

Article
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The Non-Expresser of Pathogenesis-Related Genes 1 (NPR1) is a principal regulator of plant responses to biotic and abiotic stresses. In this study, the response of ‘Hamlin’ (Citrus sinensis L.) sweet orange clones overexpressing the Arabidopsis thaliana NPR1 (AtNPR1; AT1G64280) were investigated under salt stress. Selected transgenic and non-tran...
Article
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Most of the commercially important citrus scion cultivars are susceptible to Huanglongbing (HLB), which is the most devastating disease the citrus industry has ever faced. Because the rootstock can influence the performance of the scion in various ways, including disease and pest tolerance, use of superior rootstocks can assist citrus growers with...
Chapter
Citrus is a very nutritious fruit that is consumed worldwide, making it one of the most economically important fruits. Like other crops, citrus must cope with multiple biotic and abiotic stressors in its growing environment. These stressors represent obstacles which must be overcome by horticultural improvement programs. Conventional breeding and g...
Poster
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Silicon (Si) has several beneficial effects when exogenously applied to plants, including increased tolerance against several abiotic and biotic stresses. The aim of the current study was to investigate the role of nanosize, monodisperse silica particles in mitigating the adverse effect of NaCl stress on Valencia (Citrus sinensis L.) sweet orange....
Poster
Full-text available
Successful gene transfer into the plant cell is a key requirement in the development of transgenic plants. Lemons are hard to transform citrus cultivars and commonly suffer from browning and necrosis of epicotyl segments following incubation and co-cultivation with Agrobacterium. In this study we evaluated the antioxidant compounds: lipoic acid, me...
Article
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Finger lime is a small-leaved citrus native to Australia that is becoming increasingly popular worldwide. Finger lime is monoembryonic and cannot be propagated true to type from seeds. Initial studies to develop a tissue culture protocol for the mass production of elite lines being developed in our breeding program were unsuccessful due to complete...
Article
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Climate change and rising sea levels increasingly threaten agriculture, especially in the coastal regions. Banana cultivars, especially the Cavendish clones, are sensitive to salinity stresses, which results in decreased production. In the current study, we evaluated the water deficit stress remediating effects of silicon nanopar-ticles (SiO 2 À ÀN...
Article
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Citrus cultivation is challenging due to the plethora of abiotic and biotic stresses faced by the crop. In recent years, production has been severely affected by diseases such as citrus canker and huanglongbing (HLB). Disease management is hampered as there is no field resistance to these diseases in any of the important commercially planted variet...
Article
The UF-CREC Citrus Variety Display Days have been a unique platform to fulfill the core mission of the Florida citrus industry, which is to identify new selections with superior traits to improve Florida’s competitive position in the marketplace. In the past year, five displays were conducted. In this article, we list the top 5 or 10 varieties from...
Chapter
The use of rootstocks in the evolution of citriculture has played a key role in the success of citrus industries worldwide. Rootstock has a strong influence on yield, fruit quality and tree size, and can provide tolerance to abiotic and biotic stresses. Dominant and emerging rootstocks has been described, reporting their characteristics that allow...
Chapter
Polyploidy appears to have played a limited role in citrus germplasm evolution. However, today, ploidy manipulation is an important component of citrus breeding strategies. For varieties, the main objective is to develop triploid seedless cultivars. For rootstock, the aim is to cumulate interesting traits in tetraploid hybrids and to improve adapta...
Article
Full-text available
Huanglongbing (HLB), a bacterial disease caused by Candidatus Liberibacter asiaticus (CLas), is a major threat to the citrus industry. In a previous study conducted by our laboratory, several citrus transgenic trees expressing the Arabidopsis thaliana NPR1 (AtNPR1) gene remained HLB-free when grown in a field site under high HLB disease pressure. T...
Article
Full-text available
Huanglongbing (HLB) is a disease that has devastated the Florida citrus industry, threatens the entire U.S. citrus industry, and globally is rapidly spreading. Florida’s citrus production is 90% sweet orange, which is quite sensitive to HLB. The heavy reliance on sweet orange for Florida citrus production makes the industry especially vulnerable to...
Article
Full-text available
Citrus Huanglongbing (HLB), which is also known as citrus greening, is a destructive disease continuing to devastate citrus production worldwide. Although all citrus varieties can be infected with ‘Candidatus Liberibacter asiaticus’ (CaLas), a certain level of HLB tolerance of scion varieties can be conferred by some rootstocks. To understand the e...
Article
The bacterial disease Huanglongbing (HLB) has been causing large economic losses in the citrus industry worldwide. Aimed at unravelling the mechanisms of scion/rootstock combination on improving HLB-affected orange juice quality, a specific scion/rootstock combination field trial was designed using three sibling rootstocks and two late maturing swe...
Article
Full-length and 5′ deletion fragments of three constitutively expressed gene promoters identified from the Citrus sinensis L. genome (cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1)) were evaluated for their ability to express the uidA gene in leaf, stem, and root tissues of transgen...
Article
This updated 4th edition of the Florida Citrus Rootstock Selection Guide (FLCRSG) is a revision of the 2016 publication. The guide is a convenient, easy-to-use reference to 21 characteristics of 49 rootstocks. Of those, 12 are time-honored commercial rootstocks (highlighted in blue), which are the most reliably characterized. The next 13 rootstocks...
Article
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Horticultural crops, including fruit, vegetable, and ornamental plants are an important component of the agriculture production systems and play an important role in sustaining human life. With a steady growth in the world's population and the consequent need for more food, sustainable and increased fruit and vegetable crop production is a major ch...
Article
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The large number of different rootstock varieties currently available for citrus production in Florida is unprecedented. This new 4-page article, chapter 4 of the forthcoming UF/IFAS Citrus Nursery Production Guide, provides information on rootstock uses in Florida, rootstock propagation, and the impact of tissue culture, as well as factors to cons...
Article
Full-text available
There is accumulating evidence that root system collapse is a primary symptom associated with Huanglongbing (HLB)-induced tree decline, especially for commercial sweet orange and grapefruit trees on Swingle and Carrizo rootstocks. Maintaining root health is imperative to keep trees productive in an HLB-endemic environment. Preliminary greenhouse an...
Article
Six mandarin cultivars, Ponkan ( Citrus reticulata ), Willowleaf ( Citrus deliciosa ), Kinnow ( Citrus nobilis × C. deliciosa ), Murcott (purported C. reticulata × Citrus sinensis ), W. Murcott [purported ( C. reticulata × C. sinensis ) × C. reticulata )], and Snack (purported C. reticulata hybrid), were used in protoplast fusion with different par...
Article
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Orange fruit from trees infected by 'Candidatus Liberibacter asiaticus' (CaLas) often do not look fully mature and exhibit off-flavours described as bitter, harsh, and metallic rather than juicy and fruity. Although previous studies have been carried out to understand the effect of CaLas on the flavour of orange juice using metabolomic methods, the...
Article
Genetic transformation of citrus has been mainly conducted via Agrobacterium tumefaciens using juvenile tissues of the epicotyl segments obtained from the in vitro germination of seeds. Transformation of the stem segments obtained from the mature material is an alternative to overcome or reduce the juvenile phase reported in perennial species such...
Article
The primary objective of this experiment was to determine if the selection of rootstock ( Citrus and hybrids) could enhance the development of huanglongbing (HLB)-related symptoms associated with the pathogen Candidatus Liberibacter asiaticus (CLas) in sweet orange ( Citrus sinensis ). If so, then it may permit more rapid identification of HLB-susc...
Article
Full-text available
Host disease resistance is the most desirable strategy for control of citrus canker, a disease caused by a gram-negative bacterium Xanthomonas citri subsp. citri (Xcc). However, no resistant commercial citrus cultivar has been identified. Cybridization, a somatic hybridization approach that combines the organelle and nuclear genomes from different...
Article
Anthocyanins are synthesized via the flavonoid pathway through a complex expression of several genes such as MYB transcription factors. Anthocyanins protect plants against biotic and abiotic stresses. Herein, we studied the effect of expression of MYB (VvmybA1 cloned from the red grape and Ruby cloned from ‘Moro’ blood orange) transcription factors...
Article
Full-text available
Key message Regulatory sequences from the citrus constitutive genes cyclophilin (CsCYP), glyceraldehyde-3-phosphate dehydrogenase C2 (CsGAPC2), and elongation factor 1-alpha (CsEF1) were isolated, fused to the uidA gene, and qualitatively and quantitatively evaluated in transgenic sweet orange plants. Abstract The 5′ upstream region of a gene (the...
Article
Full-text available
Long terminal repeat retrotransposons (LTR-RTs) are a large portion of most plant genomes, and can be used as a powerful molecular marker system. The first citrus reference genome (Citrus x clementina) has been publicly available since 2011; however, previous studies in citrus have not utilized the whole genome for LTR-RT marker development. In thi...
Article
Citrus tolerance to huanglongbing could result from tolerance to the pathogen Candidatus Liberibacter asiaticus (CLas) and/or to its vector Diaphorina citri. Field observations and greenhouse-controlled studies showed that some citrus cultivars were more tolerant than others. However, the mechanism(s) behind the tolerance has not been determined ye...
Article
Full-text available
Genetic engineering approaches offer an alternative method to the conventional breeding of Citrus sp. ‘W. Murcott’ mandarin (a hybrid of ‘Murcott’ and an unknown pollen parent) is one of the most commercially important cultivars grown in many regions around the world. Transformation of ‘W. Murcott’ mandarin was achieved by direct DNA uptake using a...
Article
Cylindrical Australian finger limes (Microcitrus australasica) taste like a combination of lemon, lime, and grapefruit, come in a rainbow of colors, and have a texture like caviar. Like other citrus fruits, finger limes are nutritious, low in calories, and vitamin-rich. So far in the United States only California grows finger limes commercially, bu...
Article
Full-text available
The embryo-specific Dc3 gene promoter driving the VvMybA1 anthocyanin regulatory gene was used to develop a visual selection system for the genetic transformation of citrus. Agrobacterium-mediated transformation of cell suspension cultures resulted in the production of purple transgenic somatic embryos that could be easily separated from the green...
Data
DNA maps of the Agrobacterium and protoplast vectors used in this study. (PDF)
Data
PCR results from transgenic lines regenerated from either cell suspension transformation (A) or protoplast transformation (B) and successfully acclimated to a greenhouse. (TIF)
Data
Cells used in the transformation experiment. A) Embryogenic citrus callus B) Citrus suspension cultures C) Protoplast ring in a sucrose-mannitol gradient following enzymatic digestion of suspension derived cells. (TIF)
Article
Full-text available
Abiotic and biotic stresses adversely affect the growth and productivity of plants and constitute the major yield-limiting factor in agriculture. In recent years, several transcription factors (TF) have been identified that can function as switches to control the expression of potential stress-related genes. These transcription factors play crucial...
Article
Full-text available
Citrus, embryogenic callus, suspension cells, protoplast Protoplasts are plant cells that have had their cell walls enzymatically removed. Protoplast transformation incorpo-Agrobacterium-mediated transformation. Citrus cell suspension cultures derived from totipotent embryogenic callus cells and grown in a liquid medium are commonly utilized as sou...
Article
Completely enclosed screen houses can physically exclude contact between the asian citrus psyllid [ACP (Diaphorina citri)] and young, healthy citrus (Citrus sp.) trees and prevent huanglongbing (HLB) disease development. The current study investigated the use of antipsyllid screen houses on plant growth and physiological parameters of young ‘Ray Ru...
Article
Huanglongbing (HLB) disease is a threat to most citrus (Citrus sp.) producing areas and is associated with the bacterium Candidatus Liberibacter asiaticus. The disease is transmitted by the vector asian citrus psyllid [ACP (Diaphoria citri)]. Antipsyllid screen houses can potentially reduce and eliminate HLB development in young citrus plantings by...
Article
Huanglongbing (HLB) is currently considered the most destructive disease of citrus. Since its spread to the Americas, HLB has killed millions of trees and caused a sharp decline in production in many citrus growing regions. With the continuous spread of HLB disease in Florida and worldwide, there is an urgent need for the development of commercial...
Article
Cybrid production, combining the nucleus of one species with alien cytoplasmic organelles of another, is a potentially valuable method used for improvement of various crops including Citrus species. Furthermore, this technology is considered a non-GMO biotechnology strategy. In citrus, cybrid plants can be produced as a by-product of somatic fusion...
Conference Paper
Full-text available
Under warm tropical and subtropical conditions, commercially cultivated sweet oranges and grapefruits do not produce anthocyanins. Anthocyanin pigments are produced in blood oranges primarily grown in Italy and a few other areas around the world with a mediterranean climate. These oranges require exposure to cold conditions during fruit maturity...
Article
Full-text available
Four caulimovirus-derived constitutive promoters were evaluated for gene expression in citrus and their expression levels were compared with a 35S promoter. Chimeric promoters made with duplicated enhancer sequences from the cauliflower mosaic virus (D35S), the cassava vein mosaic virus (DCsVMV), the figwort mosaic virus (DFMV), the mirabilis mosai...
Article
Full-text available
Background: Citrus blight is a citrus tree overall decline disease and causes serious losses in the citrus industry worldwide. Although it was described more than one hundred years ago, its causal agent remains unknown and its pathophysiology is not well determined, which hampers our understanding of the disease and design of suitable disease mana...
Article
Plant-based reporter gene systems enable convenient detection of gene insertion events and recovery of cisgenic/intragenic plants following transformation. For instance, anthocyanin biosynthesis genes isolated from plant species can be used as potential reporter genes for non-destructive analysis of gene expression. Anthocyanin biosynthesis genes f...
Article
Full-text available
Anthocyanins are beneficial bioflavonoids that have numerous roles in human health maintenance, disease prevention, and overall well-being. In addition, anthocyanins are key to the consumer appeal of many ornamental plants. Most Citrus (Citrus L.) plants do not produce anthocyanins under warm tropical and subtropical. conditions. Anthocyanin pigmen...
Chapter
The genus Citrus contains numerous fresh and processed fruit cultivars that are economically important worldwide. New cultivars are needed to battle industry threatening diseases and to create new marketing opportunities. Citrus improvement by conventional methods alone has many limitations that can be overcome by applications of emerging biotechno...
Article
Transgenic plants of Citrus rootstock Carrizo citrange [Citrus sinensis (L.) Osbeck × Poncirus trifoliata (L.) Raf.] expressing a p35 gene from AcMNPV (Autographa californica moth Nuclear polyhedrosis virus) were produced. The p35 gene encodes a baculovirus protein which is suggested to be a negative regulator of apoptosis. Transgenic Carrizo plant...
Article
Full-text available
Citrus allotetraploid somatic hybrids obtained via protoplast fusion have been employed as pollen/seed parents in interploidy crosses to produce triploid plants aiming to develop seedless citrus. However, their meiotic behavior, which directs the parental heterozygosity transmission, has been rarely explored. In this study, the meiotic inheritance...
Data
Validation of the applicability of the markers for estimating the allelic dosage based on MAC-PR method. a Scatter plot of peak area ratios for ten technical replications of two triploid hybrids with peak a:c = 1:1 and 2:1 for marker CV710821. b Distribution of peak area ratios of triploid hybrids showing 1:2 and 2:1 ratios for all twelve markers
Article
Full-text available
Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars 'Hamlin' and 'Valencia' expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promo...
Article
Full-text available
The most frequently used method for production of citrus transgenic plants is via Agrobacterium-mediated transformation of tissues found on explants obtained from juvenile seedlings. Within the last decade and especially within the last 5-6 years, this robust method was employed to produce thousands of transgenic plants. With the newly applied scre...
Article
In cybridization, new combinations of nuclear and cytoplasmic genes result in a unique genotype that may bring cellular, physical, physiological, and biochemical changes to the plant. This has been demonstrated in the unexpected cybrids generated from the fusion of citrus (Citrus sp.) protoplasts in two independent experiments. The first experiment...