Joseph M Jilka's research while affiliated with Texas A&M University and other places
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Publications (12)
The availability of foods low in sugar content yet high in flavour is critically important to millions of individuals conscious of carbohydrate intake for diabetic or dietetic purposes. Brazzein is a sweet protein occurring naturally in a tropical plant that is impractical to produce economically on a large scale, thus limiting its availability for...
The maize polyubiquitin-1 (Ubi-1) promoter is one of a few select promoters used to express foreign genes in monocots, such that recombinant proteins can be produced at commercially viable levels. Modifying the activity, specificity and responsiveness of such promoters provides a means to achieve desired levels and patterns of expression of genes e...
Recombinant plant expression systems offer a means to produce large quantities of selected antigens for subunit vaccines. Cereals are particularly well-suited expression vehicles since the expressed proteins can be stored at relatively high concentrations for extended periods of time without degradation and dry seed can be formulated into oral vacc...
Plants have recently become an attractive option for the production of recombinant proteins. Plant-based systems can be used to produce many classes of foreign proteins including candidate vaccine antigens. The selected antigen can be purified from plant material prior to delivery by the preferred route, or alternatively delivered orally in edible...
The synthesis of selected antigens in plants and their oral delivery has great potential for reducing the costs of vaccine production and administration. The application of this technology requires antigen concentrations in final plant material to be uniform to ensure consistent dosing. In addition, antigen levels should be such as to allow the vol...
Aprotinin is a serine protease inhibitor found in several bovine organs that has a number of applications in both the pharmaceutical and cell culture markets. In cell culture it is used as a component of serum-free media to preserve the integrity of recombinant proteins during fermentation and downstream purification. As a pharmaceutical, it is use...
The use of recombinant gene technologies by the vaccine industry has revolutionized the way antigens are generated, and has provided safer, more effective means of protecting animals and humans against bacterial and viral pathogens. Viral and bacterial antigens for recombinant subunit vaccines have been produced in a variety of organisms. Transgeni...
Advances in the development of subunit vaccines and in the production of foreign proteins in plants together offer the prospect
of stable and inexpensive vaccine delivery systems. Various bacterial and viral proteins stably produced in plants have been
shown to elicit immune responses in feeding trials. We have extended this approach by using Zea m...
Recent technological advances in transgenic plants expressing recombinant proteins have resulted in the possibility of paradigm shifts in the area of animal healthcare (Hood et al., 1997). By expressing proteins in edible tissue, the possibility of delivering a healthcare product directly by ingestion of the edible tissue is now becoming a reality....
Transmissible gastroenteritis (TGE) is a highly contagious enteric disease that is characterized by vomiting, severe diarrhea and high mortality in piglets less than two weeks of age. The development of edible vaccines offers the potential to aid in the control of enteric diseases such as TGE. Edible vaccines from plant material could be directly d...
Numerous studies have shown that viral epitopes and subunits of bacterial toxins can be expressed and correctly processed in transgenic plants. The recombinant proteins induce immune responses and have several benefits over current vaccine technologies, including increased safety, economy, stability, versatility and efficacy. Antigens expressed in...
Foreign protein production in transgenic plants has been successful, from the generation of transgenic plant lines to the marketing of purified proteins. Antigenic proteins from disease organisms, monoclonal antibodies raised against antigens of disease organisms, and proteins with industrial process applications have been produced and tested. For...
Citations
... On the other hand, recombinant aprotinin (polypeptide, 58 amino acid residues), as a classic example of a serine proteinase inhibitor, accumulated in plants at a high level-up to 9% of TSP in corn seeds (Delaney et al. 2003;Karg and Kallio 2009), which indicates different possibilities for the synthesis and accumulation of protein molecules of various composition and/or mass in plants. ...
... Expression of animal vaccine components in seeds of transgenic plants was attempted quite early on, with Lamphear et al. (2002) in 2002 reviewing their own earlier work on maize seed expression of the B subunit of E coli heat-labile enterotoxin and the TGEV S protein, with data on the potency, efficacy, and stability of these vaccines. Another report followed in 2002 on the expression in maize seed of the S envelope protein of transmissible gastroenteritis coronavirus (TGEV) of swine, and its protective efficacy in piglets fed with the seed (Jilka 2002). This followed an earlier demonstration of oral immunogenicity of the S protein N-terminal domain in transgenic potato tubers (Gomez et al. 2000). ...
... Arabidopsis seeds, as high as 12.5 % and 36.5% of total soluble proteins using the 5' and 3' regulatory sequences of the seed storage protein gene arcelin 5-I and beta-phaseolin proinsulin was efficiently expressed in maize plants (Streatfield et al. 2002;Chickwamba et al. 2002;Lamphear et al. 2004;Streatfield, 2005;Farinas et al. 2007;Karaman et al. 2012). ...
... Plants have been used to produce proteins from different species for some time now. 19,20 The use of enzymes made in transgenic plants can cut the cost of many processes like the bleaching of paper. 21 The advantages of this method are that the plants can be grown in fields, which is less costly than the millions of dollars needed to build facilities for fermentation. ...
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... Plants have been used as manufacturing hosts for treatments and subunit vaccines for more than 25 years [16,17]. Transient protein expression in Nicotiana benthamiana is an important platform for producing plant-made pharmaceuticals [18]. ...
... Immunizations against transmissible gastroenteritis virus (TGEV) in swine have historically had issues in inducing immune protection [156,157], but are widely used now. Too-frequent exposure to vaccine antigens can lower the immune response against TGEV [158]. Another swine coronavirus vaccine, porcine epidemic diarrhoea virus (PEDV), is widely used [159]. ...
... In direct plastid transformation of cry9Aa2 gene in tobacco, [12] also detected morphological alterations when the transgene is expressed at high concentrations. The endoplasmic reticulum (ER) has been favoured for targeting different proteins to obtain elevated protein levels and biological activity in plants [19][20][21][22] However, this option was not explored for the expression of Bt proteins. In the present study, we have targeted truncated Cry1Ac to the cytoplasm, routed it through ER, and targeted it to the chloroplast to investigate the effects of delta-endotoxin targeting on protein expression levels, stability, and biological activity. ...
... Later, the S1 glycoprotein of the infectious bronchitis virus (IBV) was selected for expression in transgenic potato, which induced desirable immunogenicity in mice and chickens against CoVs when orally administered, comparable to other commercial vaccines [217,218]. Similarly, the spike protein of the transmissible gastroenteritis virus (TGV) was used to produce an oral vaccine in corn seed, further boosting immunity in swine against CoV [219]. However, very limited experiments have been conducted targeting Human coronaviruses (HCoVs) using plantbased vaccines. ...
... Another study successfully analyzed when plant-produced HBsAg is administered orally, it can trigger an immune response. It was realized that the identification of an immunogenic HBV protein that may activate the human immune system to create defensive antibodies is a major determinant in the development of a HBV vaccine (Horn et al., 2003). Furthermore, one must optimize and construct a quantifiable measure of vaccination success, which necessitates the optimization of dosage levels and timings (Khadwal et al., 2020). ...
... This 5' leader sequence has a poly CAA region, which acts as a binding site for heat shock protein (HSP 101).We express the vip3Aa86 transgene under the influence of maize polyubiquitin-1 promotor. This promoter is widely used for driving high levels of gene expression in monocots including maize (Streatfield et al. 2004;Bhatti et al. 2020). ...
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