José Luis Iriarte’s research while affiliated with Universidad Austral de Chile and other places

The Magellan Subantarctic ecoregion (MSE) in the Southern Hemisphere (47°-56°S; 71°-73°W) is a unique natural laboratory subject to persistent and accelerated glacial ice melt, generating a complex system of environmental gradients (e.g., salinity and temperature) that influence the ecological patterns of marine biodiversity. However, the factors influencing marine epiphytic microalgal assemblages are still poorly understood. In this context, we characterized the richness and structure of epiphytic assemblages in different benthic macroalgal hosts (Acrosiphonia arcta, Ectocarpus siliculosus, and Leptosiphonia brodiei) in sites with glaciers and estuarine characteristics (Yendegaia Bay and Fouquet Estuary) and sites without glaciers and oceanic characteristics (Batchelor River and Offing Island) of the MSE, revealing how sites, host, and environmental variables influence variation of epiphytic assemblages. In 36 samples, 67 genera of epiphytes were recorded. The dominant divisions were Bacillariophyta (50 genera), Dinophyta (7 genera) and Cyanophyta (6 genera). We observed significantly high diversity in epiphytic assemblages, with contrasting patterns of variation depending on site and/or host macroalgae. Host specificity was not evident for most epiphytes. The most factor influencing the variation of the epiphythic assemblage was the marked environmental gradient (changes in temperature, salinity, nutrients, among others) between sites with and without glacial influence. Additionally, our research identified potentially toxic and/or harmful epiphytic microalgae belonging to the divisions Dinophyta (dinoflagellates) and Cyanophyta (cyanobacteria). The data on ecological patterns of epiphyte assemblages provides valuable insights into the current state of a poorly understood microscopic biodiversity, shaped by diverse environmental factors at different sites. Under current and future climate change scenarios in the MSE, environmental gradients may become more pronounced, with important positive and/or negative consequences on epiphyte assemblages. In light of these findings, we present a baseline for future research to further develop our understanding and facilitate the monitoring and conservation of epiphytic microalgae in the MSE. 50-day free acess to the full article provided at: https://authors.elsevier.com/c/1k2hZB8cd0XUC

Microbes in marine ecosystems have evolved their gene content to thrive successfully in the cold. Although this process has been reasonably well studied in bacteria and selected eukaryotes, less is known about the impact of cold environments on the genomes of viruses that infect eukaryotes. Here, we analyzed cold adaptations in giant viruses (Nucleocytoviricota and Mirusviricota) from austral marine environments and compared them with their Arctic and temperate counterparts. We recovered giant virus metagenome-assembled genomes (98 Nucleocytoviricota and 12 Mirusviricota MAGs) from 61 newly sequenced metagenomes and metaviromes from sub-Antarctic Patagonian fjords and Antarctic seawater samples. When analyzing our data set alongside Antarctic and Arctic giant viruses MAGs already deposited in the Global Ocean Eukaryotic Viral (GOEV) database, we found that Antarctic and Arctic giant viruses predominantly inhabit sub-10°C environments, featuring a high proportion of unique phylotypes in each ecosystem. In contrast, giant viruses in Patagonian fjords were subject to broader temperature ranges and showed a lower degree of endemicity. However, despite differences in their distribution, giant viruses inhabiting low-temperature marine ecosystems evolved genomic cold-adaptation strategies that led to changes in genetic functions and amino acid frequencies that ultimately affect both gene content and protein structure. Such changes seem to be absent in their mesophilic counterparts. The uniqueness of these cold-adapted marine giant viruses may now be threatened by climate change, leading to a potential reduction in their biodiversity.

The Magellan Subantarctic ecoregion (MSE) in the Southern Hemisphere (47°-56°S; 71°-73°W) is a unique natural laboratory undergoing rapid environmental change, offering an exceptional study region to observe the effects of environmental gradients on marine biodiversity and marine ecosystem functioning. In the coastal benthic ecosystems of the MSE, the ecological interaction between macroalgal and microalgal species has been poorly studied to date. Here, we present the first ecological description of epiphytic microalgal assemblages on benthic macroalgal hosts from contrasting MSE sites featuring glaciers with estuarine characteristics (Yendegaia Bay and Fouquet Estuary) and glacier-free areas with oceanic characteristics (Batchelor River and Offing Island). Epiphyte assemblages were also described between different macroalgal hosts (Acrosiphonia arcta, Ectocarpus siliculosus and Leptosiphonia brodiei). In 36 samples, 67 genera of epiphytes were recorded. The dominant divisions were Bacillariophyta (50 genera), Dinophyta (7 genera) and Cyanophyta (6 genera). Significant differences in the richness, diversity and relative abundance of epiphytic microalgae were observed as a function of site location and macroalgal host. When compared to local abiotic factors (temperature, salinity, dissolved oxygen and nutrients), our results indicated that marked environmental gradients between sites with and without glacial influence shape the biodiversity of epiphytic microalgal assemblages. The division Bacillariophyta (diatoms) was dominant within the epiphytic microalgal assemblages on macroalgal hosts regardless of the glacial influence, as well, potentially toxic and/or harmful epiphytic microalgae belonging to the divisions Dinophyta (dinoflagellates) and Cyanophyta (cyanobacteria) were recorded at all sites and on all hosts. These findings contribute to understanding the biodiversity and dynamics of epiphytic microalgae in the MSE, particularly in relation to different environmental stressors associated with climate change scenarios. In addition, we discuss how the presence of epiphytic microalgal species may contribute to the formation of benthic Harmful Algal Blooms (bHABs), with potential ecological, economic and social consequences.

Subtropical‐subpolar transition water is a potential domain for N2 fixation, but the understanding of N2 fixation in such waters remains incomplete. We simultaneously examined the N2 fixation activity and community structures of diazotrophs and all prokaryotes from the surface to just above the seafloor off Patagonia in the transitional region of the eastern South Pacific Ocean. N2 fixation activity was not detected in the surface waters, but was observed sporadically and only in subpolar bathypelagic waters (>1,000 m) at very low rates (0.02–0.06 nmol N L⁻¹ d⁻¹). By contrast, the nifH gene, a key gene involved in N2 fixation, was detected widely from the surface to the bottom waters. The majority of diazotrophs were classified as non‐cyanobacterial diazotrophs (NCDs), and the nifH amino acid sequences of major diazotrophs were similar to sequences detected in the Southern Ocean, the aphotic zone and sediment of other oceans, and estuarine waters, suggesting that the NCDs are distributed across diverse marine environments. The overall prokaryotic communities were generally similar to those in other open ocean regions at the phylum level (class level for Proteobacteria) and differed among water depths. Diazotrophs, in contrast, showed vertical and horizontal heterogeneity below the euphotic zone and little association with water depth, indicating a lack of cohesion within the community, which may characterize diazotroph community in the transitional surface water and aphotic zones. Elucidating this community heterogeneity may provide pivotal information about N2 fixation in these waters.

The Chilean Patagonian fjords are globally renowned as one of the few remaining pristine environments on Earth; however, their ecosystems are under significant threat from climatic and anthropogenic pressures. Of particular concern is the lack of research into the impact of plastic pollution on the waters and biodiversity of these fjords. In this study, the marine environment of a secluded and sparsely populated fjord system in southern Patagonia was sampled to assess microplastics in seawater, beaches, bottom sediment, and zooplankton. Microplastics were found to be widespread across the water surface of the fjord, but with low abundances of 0.01 ± 0.01 particles m−3 (mean ± SD). The presence of microplastics in sedimentary environments (e.g., beaches and bottom sediments, 15.6 ± 15.3 and 9.8 ± 24 particles kg of dry sediment−1, respectively) provided additional evidence of plastic debris accumulation within the fjord system. Furthermore, microplastics were already bioavailable to key zooplankton species of the Patagonian food web (0.01 ± 0.02 particles individual−1), suggesting bioaccumulation. A comprehensive examination of potential microplastic inputs originating from coastal runoff, coupled with distribution of water masses, suggested minimal local contribution of microplastics to the fjord, strongly indicating that plastic litter is likely entering the area through oceanic currents. The composition and type of microplastics, primarily consisting of polyester fibers (approx. 60 %), provided further support for the proposed distant origin and transportation into the fjord by oceanographic drivers. These results raise significant concern as reveal that despite a lack of nearby population, industrial or agricultural activity, remote Patagonian fjords are still impacted by plastic pollution originating from distant sources. Prioritizing monitoring efforts is crucial for effectively assessing the future trends and ecological impact of plastic pollution in these once so-called pristine ecosystems.

Harmful algal blooms (HABs) have been a persistent phenomenon in Patagonian fjords and channel system for more than 50 years. The occurrence of marine toxins produced by microalgae in Chile was officially recognized in 1972. Recent natural and anthropogenic threats to the marine system in Chilean Patagonia have led to changes in terrestrial and aquatic ecosystems. The unprecedented retreat of ice fields in the southernmost regions has resulted in increased freshwater runoff into adjacent glacier and fjord system. In this study, we present a comprehensive review of historical data and recent experiments, including the results from the 2019 PROFAN cruise. We develop a hypothetical and conceptual model to illustrate climate-hydrologic-ocean interactions with implications for the estuarine environment of the Magellan region. We include two scenarios that consider initiation processes, phytoplanktonic succession associated with the progression of HAB events in the southernmost fjords. These processes include haline stratification, temperature, seasonal availability of light and nutrients availability, with effects on plankton community structure along a longitudinal gradient. The results also highlight the impact of HABs on the aquaculture industry and the need for mitigation and adaptation strategies and provide new insights into HABs in Patagonia`s inland seas of and suggest the prevailing environmental conditions that would favor the appearance of new HAB species.

The biogeochemical dynamics of fjords around Antarctica are strongly influenced by cryospheric, climatic, and oceanographic processes that occur on a seasonal scale. Furthermore, as global climate change continues, there is a growing awareness of the impact of ocean warming on glacier melting, which is expected to affect the composition of phytoplankton community structure in West Antarctica’s nearshore marine areas. In this study, we describe the role of hydrographic forcing on the short-term summer variability of the phytoplankton community in Marian Cove, an Antarctic glacial fjord (62°S). Phytoplankton and hydrographic variables were measured at five stations along the Marian Cove during summer 2019 (January–February). The highest concentrations of microphytoplankton biomass were found in the outer area of the fjord, whereas nanophytoplankton biomass displayed continued dominance during most of the summer period in Marian Cove. Hydrographic assessment showed that freshwater inputs from the glacier influenced the surface layer of the fjord, modulating phytoplankton biomass, which was dominated by nanodiatoms (Minidiscus sp., Thalassiosira spp.) and nanophytoflagellates (Cryptomonas spp., Phaeocystis sp.). Concurrent measurement of phytoplankton biomass and environmental conditions during December 2018–January 2019 indicated that a period of weak southeastern winds generated vertical stability, which led to the development of a major peak of microphytoplankton biomass in the outer cove, driven by warm, allochthonous, oceanic, nutrient-rich waters. High carbon biomass dominated by nanodiatoms and nanophytoflagellates was observed in cold, fresh, and low-light subsurface waters of the cove. Our results highlight the effects of a warming ocean, which may favor the summer resurgence of nanodiatom and nanophytoflagellate communities in Antarctic fjords due to increased glacial meltwater inputs.

[This corrects the article DOI: 10.3389/fmicb.2022.862812.].