José L. Sanz's research while affiliated with Universidad Autónoma de Madrid and other places
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Publications (57)
Tirez was a small and seasonal endorheic athalassohaline lagoon that was located in central Spain. In recent years, the lagoon has totally dried out, offering for the first time the opportunity to analyze its desiccation process as a “time-analog” to similar events occurred in paleolakes with varying salinity during the wet-to-dry transition on ear...
The presence of 1,4 dioxane in wastewater is associated with severe health and environmental issues. The removal of this toxic contaminant from the industrial effluents prior to final disposal is necessary. The study comprehensively evaluates the performance of sequential batch membrane bioreactor (MBR) for treating wastewater laden with 1,4 dioxan...
Tirez was a small and seasonal endorheic athalassohaline lagoon that was located in central Spain. In recent years, the lagoon has totally dried out, offering for the first time the opportunity to analyze its evolution to hyperaridity as an analog to similar processes in Meridiani Planum, Mars. In Meridiani, an early period of water ponding within...
Herein, we report a novel supplement called Cu@Fe3O4 core shell
nanostructure (NS) that revealed a tremendous increment in the biogas production from anaerobic digestion of sewage sludge. Cu@Fe3O4 core-shell NS is synthesized using feasible co-precipitation method and characterized using different techniques before and after anaerobic digestion of...
Herein, we report a novel supplement called Cu@Fe3O4 core shell nanostructure (NS) that revealed a tremendous increment in the biogas production from anaerobic digestion of sewage sludge. Cu@Fe3O4 core-shell NS is synthesized using feasible co-precipitation method and characterized using different techniques before and after anaerobic digestion of...
With escalating global demand for renewable energy, exploitation of farm wastes (i.e., agriculture straw wastes (ASWs), livestock wastewater (LW) and sewage sludge (SS)) has been considered to attain maximum methane yield (MY) via anaerobic digestion (AD). Results pointed that mixture of SS and LW as anaerobes’ source with 20 g of ASWs/300 mL of wo...
The bone histology of non-avian theropods such as Troodon, early pygostylians such as Confuciusornis, and neornithines, is characterized by the post-hatching formation of fibrolamellar complex. In contrast, the cortex of enantiornithine birds, like Concornis and Iberomesornis, is made of poorly vascularized parallel-fibred tissue. The cortex of met...
The Dallol geothermal area originated as a result of seismic activity and the presence of a shallow underground volcano, both due to the divergence of two tectonic plates. In its ascent, hot water dissolves and drags away the subsurface salts. The temperature of the water that comes out of the chimneys is higher than 100 °C, with a pH close to zero...
Most of the terrestrial deep subsurfaces are oligotrophic environments in which some gases, mainly H2, CH4, and CO2, play an important role as energy and/or carbon sources. In this work, we assessed their biotic and abiotic origin in samples from subsurface hard‐rock cores of the Iberian Pyrite Belt (IPB) at three different depths (414, 497, and 52...
Ten efficient hydrogen-producing strains affiliated to the Clostridium genus were used to develop con-sortia for hydrogen production. In order to determine their saccharolytic and proteolytic activities, glucose and meat extract were tested as fermentation substrates, and the best hydrogen-producing strains were selected. The C. roseum H5 (glucose-...
Microorganisms are responsible for the conversion and breakdown of organic compounds and contaminants in bioreactors designed for the treatment of different types of waste. Organized in highly complex communities, they are the heart of every wastewater treatment plant and solid residue landfill. The detailed characterization of these communities an...
Concavenator corcovatus is represented by a single and almost complete and articulated skeleton, MCCM-LH 6666, from the Las Hoyas fossil site (Lower Cretaceous, Spain). The axial skeleton only lacks some caudal vertebrae whereas presacral and sacral regions are totally articulated. Concavenator shows several unusual features associated to the axial...
Concavenator corcovatus is a carcharodontosaurid dinosaur represented by an almost complete and articulated skeleton from the ‘Las Hoyas’ fossil site (Lower Cretaceous, Spain). The skull of Concavenator is almost complete, missing only the anteriormost and posteriormost regions. Here, a review of the cranial anatomy of Concavenator has been carried...
Two deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques a...
Intermittent flight through flap-gliding (alternating flapping phases and gliding phases with spread wings) or bounding (flapping and ballistic phases with wings folded against the body) are strategies to optimize aerial efficiency which are commonly used among small birds today. The broad morphological disparity of Mesozoic birds suggests that a r...
While environmental factors affect animal locomotion (especially among fliers), few studies have addressed the relationship between aerial function in extinct vertebrates and paleoatmospheric conditions, and none have focused on avian flight. Our study explores the impact of varying O2 concentrations, global temperatures, and air densities on the f...
Here, we report the complete genome sequence of Tessaracoccus sp. strain T2.5-30, which consists of a chromosome with 3.2 Mbp, 70.4% G+C content, and 3,005 coding DNA sequences. The strain was isolated from a rock core retrieved at a depth of 139.5 m in the subsurface of the Iberian Pyritic Belt (Spain).
13-Propanediol (13-PDO) was obtained from glycerol fermentation through a microbial mixed culture and consequently established the operational conditions of two up-flow anaerobic sludge blanket (UASB) reactors to achieve a maximum 13-PDO yield. The UASB reactors with initial pH values set at 6.8 and 5.5 were operated at 30°C during 165 d. Yeast ext...
Our knowledge of the diversity, ecology, and phylogeny of Mesozoic birds has increased significantly during recent decades, yet our understanding of their flight competence remains poor. Wing loading ( WL ) and aspect ratio ( AR ) are two aerodynamically relevant parameters, as they relate to energy costs of aerial locomotion and flight maneuverabi...
A new dryosaurid ornithopod, Eousdryosaurus nanohallucis, gen. et sp. nov., is described here based on a single specimen from the Late Jurassic Alcoba¸ca Formation of Portugal. Eousdryosaurus nanohallucis is distinguished from all other dryosaurids by eight autapomorphic features and an unique combination of characters, some of which are also share...
The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production...
This article describes isolated skull bones of at least three ornithopod dinosaurs from the lower Aptian “Arcillas de Morella” Formation at Morella (Castellón, Spain). These bones correspond to two right maxillae and a partial left quadrate. Analysis of the two maxillae belonging to the large-sized European ornithopod Iguanodon bernissartensis prov...
Phylogenetic tree of the archaeal community from sludge samples during the dry (SD) and rainy (SR) seasons, which was constructed using the neighbor-joining method. The numbers at the nodes indicate the percentage of occurrence in 1,000 bootstrapped trees. Methanopyrus kandleri (U57340) was used as the outgroup. Supplementary material 3
We investigated the microbial community in an up-flow anaerobic sludge blanket (UASB) reactor treating domestic wastewater (DW) during two different periods of organic loading rate (OLR) and food-to-microorganism (F/M) ratio. 16S rDNA clone libraries were generated, and quantitative real-time PCR (qPCR) analyses were performed. Fluctuations in the...
Ten efficient hydrogen-producing strains affiliated to the Clostridium genus were used to develop consortia for hydrogen production. In order to determine their saccharolytic and proteolytic activities, glucose and meat extract were tested as fermentation substrates, and the best hydrogen-producing strains were selected. The C. roseum H5 (glucose-c...
Acid mine drainage (AMD) - characterized by high acidity and elevated sulfate and metal concentrations - represents a big environmental concern. Biological sulfate reduction has become an alternative to the classical physicochemical methods. In this study, domestic wastewater (DW) was tested as a cost-effective carbon-source for the remediation of...
Over the last decades, the demand for pork products has increased significantly, along with concern about suitable waste management. Anaerobic-lagoon fermentation for swine-sludge stabilization is a good strategy, although little is known about the microbial communities in the lagoons. Here, we employed a cloning- and sequencing-based analysis of t...
The prokaryotic diversity of an anaerobic reactor for the treatment of municipal solid waste was investigated over the course of 2 years with the use of 16S rDNA-targeted molecular approaches. The fermentative Bacteroidetes and Firmicutes predominated, and Proteobacteria, Actinobacteria, Tenericutes and the candidate division WWE1 were also identif...
Archaeopteryx has played a central role in the debates on the origins of avian (and dinosaurian) flight, even though as a flier it probably represents a relatively late stage in the beginnings of flight. We report on aerodynamic tests using a life-sized model of Archaeopteryx performing in a low turbulence wind tunnel. Our results indicate that tai...
Río Tinto (Iberian Pyritic Belt, SW Spain) is well known for its low pH (mean pH 2.3), high redox potential (> +400 mV) and high concentration of heavy metals. In this work we describe and analyse the presence of methanogenic archaea in the extreme acidic and oxidizing environment of the Tinto basin. Methane formation was measured in microcosms ino...
The sensitivity of seven extremely halophilic archaeal protein synthesis systems to 42 inhibitors with different domain (kingdom), functional, and structural specificity has been studied. The results obtained underline the functional variability of archaeal ribosomes when compared with bacterial and eukaryotic reference systems. Halophilic ribosome...
Carcharodontosaurs were the largest predatory dinosaurs, and their early evolutionary history seems to be more intricate than was previously thought. Until recently, carcharodontosaurs were restricted to a group of large theropods inhabiting the Late Cretaceous Gondwanan land masses, but in the last few years Laurasian evidence has been causing a r...
Polycyclic aromatic hydrocarbons (PAH; naphthalene, anthracene and phenanthrene) degrading microbial consortium C2PL05 was
obtained from a sandy soil chronically exposed to petroleum products, collected from a petrochemical complex in Puertollano
(Ciudad Real, Spain). The consortium C2PL05 was highly efficient degrading completely naphthalene, phen...
In this work we study twenty teeth of spinosaurid theropod dinosaurs from the Early Aptian (Early Cretaceous) of Morella (Castellón, Spain). The teeth exhibit a mosaic of derived theropod characters such as ornamentation in longitudinal crests together with very small, uniformly sized denticles, which resemble baryonychine spinosaurids such as Bary...
The aim of the present study was to assess the impact of adding cultures of Thiobacillus denitrificans and Thiomicrospira denitrificans to two upflow anaerobic sludge bed (UASB) reactors: one inoculated with granular sludge and the other filled only with activated carbon (AC). The performances of the bioreactors and the changes in biomass were comp...
The formation, structure, and biodiversity of a multispecies anaerobic biofilm inside an Upflow Anaerobic Sludge Bed (UASB) reactor fed with brewery wastewater was examined using complementary microbial ecology methods such us fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE), and cloning. The biofilm develop...
Identification of microorganisms by conventional methods requires the isolation of pure cultures followed by laborious characterization experiments. These procedures are therefore inadequate for study of the biodiversity of a natural or engineered ecosystem. A new set of molecular techniques developed during the 1990s revolutionized microbial ecolo...
Methanogenic granules from an anaerobic bioreactor that treated wastewater of a beer brewery consisted of different morphological
types of granules. In this study, the microbial compositions of the different granules were analyzed by molecular microbiological
techniques: cloning, denaturing gradient gel electrophoresis and fluorescent in situ hybri...
The anaerobic biodegradation of Linear Alkylbenzene Sulfonate (LAS) was studied in Upflow Anaerobic Sludge Blanket Reactors (UASB). One reactor was fed with easily degradable substrates and commercial LAS solution during a period of 3 months (Reactor 1), meanwhile a second reactor was fed with a commercial LAS solution without co-substrate (Reactor...
We have discovered a mass of fossil bones from four juvenile birds at
Las Hoyas in Cuenca, Spain, which show signs of having been digested. To
our knowledge, this rare finding of an Early Cretaceous fossil of an
apparently regurgitated pellet provides the first evidence that Mesozoic
birds were prey animals.
A feathered skeleton of a Lower Cretaceous enantiornithine bird from Spain indicates that the modified diapsid skull of modern
birds did not evolve until late in their evolution: Basal birds retained an essentially primitive diapsid design. The fossil
provides data clarifying long-standing debates on the cranial morphology of the basalmost bird, Ar...
AVIAN flight is one of the most remarkable achievements of vertebrate evolution, yet there is little evidence of its early phases. Specimens of Archaeopteryx shed important (albeit controversial) light on this evolutionary phenomenon, but the large morphological (and almost certainly functional) gap between Archaeopteryx and modern avians remained...
Although famous as a fossil lagerstatte that has produced numerous well-preserved vertebrates, recent discoveries indicate that the lithographic limestones of Las Hoyas (Calizas de la Huerguina Formation) also contain vertebrate trackways. We herein report on at least two distinctive tetrapod track types tentatively assigned to crocodilians and to...
A dinosaur tracksite has been discovered comprising three types of tridactyl footprints at Regumiel de la Sierra (province of Burgos, north‐central Spain). The rocks belong to Lower Cretaceous Wealden deposits of the Western Cameros Basin and two ichnological levels comprising 8 trackways and several isolated tracks were noted.All the footprints ar...
Concornis lacustris. a basal bird from Lower Cretaceous (Barremian) beds of Las Hoyas (Spain). was previously interpreted as the sister-group of a clade formed by Ambiortus dementjevi, Enantiornithes, and Ornithurae (Hesperornithiformes, Ichthyornis and modern birds). Further preparation of the holotype specimen, however, shed new light on its rela...
The discrimination of ornithopod from theropoddinosaur footprints can sometimes be problematical. In order to obtain discriminatory criteria, an analysis BMDP (4M, 7M) on a sample of 66 Lower Cretaceous tridactyl footprints of diverse geographical origins has been carried out. The more significant ichnological parameters explaining sample variabili...
Citations
... These drawbacks of the AS technology push scientists to use a novel technology like membrane bioreactor (MBR). MBR has many advantages such as a small footprint, high solid-liquid separation and production of good effluent quality Tawfik et al., 2022). When MBR operates, the solid-liquid separation takes place across membrane filtration. ...
... This Egyptian obstacle represents a big challenge for achieving the sustainability aims, results in a serious health and environmental problems for the society [1]. Anaerobic digestion (AD) is considered one of the most widely methods for treating of domestic wastewater [2], industrial wastewater [3], solid waste [4], agriculture waste [5] and sewage sludge [6] prior to discharge into water bodies. One of the benefits of using the anaerobic digestion process for treating different aspects of wastewater, including sewage sludge, is the produced biogas that could be used as a new renewable energy source and could be exploited for producing heat, steam, electricity, or even vehicle fuel [7,8]. ...
... Numerous studies like 5 have been conducted on photocatalytic oxidation of organic compounds to CO 2 . Recently, many novel nanoparticles have been fabricated for environmental applications 6 . The most popular nano-catalyst and photo-catalyst in the same time is titanium (TiO 2 ) because it is readily available and stable under a variety of reaction settings 7 . ...
... Our finding confirmed that the TiO 2 NPs and TiO 2 @Ag core-shell NS promoted the transformation of organic compounds to bio-methane via acetoclastic methanogens, which may be the reason for the increasing bio-methane production with consuming all acetate groups. These findings were comparable to those of Tawfik et al. [54] who indicated that percent of 98 % from acetic acid has been removed in the anaerobic digestion process via acetolactic methanogens. The highest removal of T.VFAs (86.7 %) was obtained after using TiO 2 @Ag core-shell NS and this enhancement could be due to the increment of the TiO 2 NPs activity after doping with Ag NPs ions as shown in Fig. 7. ...
... This is the case of the FISH study in a Dallol brine (D10) by Gómez et al. (57). Doubt can be also cast on another study claiming the extensive occurrence of methanogenesis at high temperature, high ionic strength, and pH close to zero in Dallol brines (60). In that study, the authors inoculated Dallol brines on culture media for methanogenic archaea and incubated these inoculated media at 45°C and 95°C for 6 months. ...
... in turn affect local hydrogeochemistry Casar et al., 2020;Mehrshad et al., 2021;Sahu et al., 2022;Soares et al., 2023). Microcosms mimicking the in situ geochemistry offer a possibility to study the development of biofilm communities and their interactions with the available mineral surfaces, and long-term incubations facilitate observations of changing dynamics in the rockwater interphase dwelling microbial communities (Leandro et al., 2018;Sanz et al., 2021;Nuppunen-Puputti et al., 2022). As energy and carbon sources are limiting factors in the deep subsurface as well as in long microcosm incubation simulations, the microbial genera able to use rock surfaces as their nutrient source are more likely to thrive. ...
... The developments in wastewater treatment in the last 100 years or so, have been possible in accordance with the accuracy and polishing of the models which represent the hydraulics of the reactors as well as the chemical and biochemical reactions that take place inside. Almost all unit operations in wastewater treatment plants are reactors: (a) pretreatment unit operations such as screening, comminution, sedimentation for grit removal, skimming, and flow equalization; (b) primary treatment unit operations such as sedimentation and flotation; (c) secondary treatment unit operations such as biological processes (aerobic or anaerobic); (d) tertiary treatment unit operations such as filtration, air stripping, adsorption, ion exchange, chemical precipitation, chemical oxidation, and disinfection processes including chlorination, ozonation or UV irradiation [5][6][7][8]. While in the pretreatment and primary treatment unit operations, the driving forces inside the reactors are physical whose purpose is mainly to separate suspended solids from water [9,10], in the secondary treatment the reactors are designed to guarantee the best conditions for biological treatment (i.e., organic matter oxidation, nitrification, and denitrification). ...
... Insectivory has also been attributed to the small crocodyliform Cassissuchus(Buscalioni 2017; Fig. 6) and the medium-sized and ornithomimid dinosaur Pelecanimimus, according to its unique dentition (multitoothed rows, with dozens of small teeth)(Pérez-Moreno et al. 1994). The status of Tertiary consumer remains difficult to allocate to any taxa in the land environment because there are no strictly identifiable carnivore tetrapods; the carcharodontosaurid Concavenator(Ortega et al. 2010;Cuesta et al. 2019), the largest animal recorded at Las Hoyas(Fig. 6), arguably represented a Quaternary top consumer in the system. ...
... Many large theropods, including Allosaurus, Torvosaurus, and Ceratosaurus, in the Morrison Formation, but also Monolophosaurus, Carcharodontosaurus, Concavenator, Cryolophosaurus, Dilophosaurus, Giganotosaurus, and other carnosaurs elsewhere, possessed decorative head ornamentation and other elaborate features that would have been obstacles to animals that relied on stealth during ambush, stalking, or pursuit behaviors (Gates et al., 2016). The same is true of modern vultures, which often exhibit bizarre display features that serve to help identify conspecifics and to intimidate competitors at carcass sites, and interestingly, this also may have been the case in Thylacosmilus (Graves, 2016;Cuesta et al., 2018;Janis et al., 2020). ...
... in turn affect local hydrogeochemistry Casar et al., 2020;Mehrshad et al., 2021;Sahu et al., 2022;Soares et al., 2023). Microcosms mimicking the in situ geochemistry offer a possibility to study the development of biofilm communities and their interactions with the available mineral surfaces, and long-term incubations facilitate observations of changing dynamics in the rockwater interphase dwelling microbial communities (Leandro et al., 2018;Sanz et al., 2021;Nuppunen-Puputti et al., 2022). As energy and carbon sources are limiting factors in the deep subsurface as well as in long microcosm incubation simulations, the microbial genera able to use rock surfaces as their nutrient source are more likely to thrive. ...