Jitka Klikarová's research while affiliated with University of Pardubice and other places
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Publications (8)
Coffee is a very popular beverage worldwide. However, its composition and characteristics are affected by a number of factors, such as geographical and botanical origin, harvesting and roasting conditions, and brewing method used. As coffee consumption rises, the demands on its high quality and authenticity naturally grows as well. Unfortunately, a...
The nasturtium (Tropaeolum majus L.) contains many biologically active compounds with very promising effects on human health. Our attention was paid to glucotropaeolin and phenolic compounds that were simultaneously determined in different parts of nasturtium using rapid reversed-phase high performance liquid chromatography coupled to tandem mass s...
Two catecholase-like biomimetic catalysts, namely, two dinuclear copper complexes [Cu2(L1)(OH)(H2O)(EtOH)][ClO4]2 (C1) and [Cu2Ac2O(L1)ClO4] (C2) with the 2,6-bis(4-methyl piperazin-1-yl-methyl)-4-formyl-phenoxy ligand (L1) together with the mononuclear complex Cu(ClO4)2(L2) (C3) containing ligand 1,2-(C5H4N-6-OCH3-2-CHN)2CH2CH2 (L2), were synthes...
This work deals with the rapid simultaneous determination of caffeine and the three most abundant chlorogenic acid isomers (3-, 4-, and 5-O-caffeoylquinic acids) present in 64 samples of regular (un)roasted and decaffeinated (un)roasted coffee beans using reversed-phase high-performance liquid chromatography with spectrophotometric detection. In th...
Amino acids (AAs) are minor compounds occurring in meads contributing to their final organoleptic properties. Determination of AAs profile and content can help to assess the mead authenticity, adulteration and thus its quality. This work deals with the optimization of rapid analysis of 21 AAs present in mead using reversed-phase high performance li...
The content of 40 phenolic compounds was determined in 68 samples of extra virgin olive oils (EVOOs) coming from 9 Italian regions using high-performance liquid chromatography coupled to diode array detector and mass spectrometry. Oleuropein isomers (M = 540 g/mol) and derivatives (M = 378 g/mol) together with ligstroside aglycone isomers (M = 362...
Extra virgin olive oils (EVOOs) contain various bioactive compounds belonging to phenol family. These compounds are traditionally determined by liquid chromatography, despite such a method implies some issues that have to be taken into account. Therefore, our study was focused on the employment of reversed-phase high-performance liquid chromatograp...
A completely new voltammetric method has been developed for quantitative determination of food additive Taurine (Tau)in energy drinks. This electroanalytical method is based on voltammetric oxidation of o-phthalaldehyde-ethanthiol derivative of Tau at glassy carbon electrode in 95% methanol containing 0.1 mol L ⁻¹ lithium perchlorate. Working condi...
Citations
... Therefore, it is essential that analytical techniques are able to detect various forms of adulteration (the use of poor-quality coffee beans, such as unripe, burnt, defective, etc. [10], presence of specific adulterants (Figure 2), degree of dilution, and unauthorized use of geographic origin of coffee beans [112,115,116]) to find whether the product label claims are based on the truth. For these reasons, various spectroscopic [12,63,64,[67][68][69][70][71]75,[80][81][82][106][107][108]113,[117][118][119][120], electrophoretic [87,109], electrochemical [110,114], and biological [102,109,111,121] techniques have already been developed, but chromatographic techniques, especially high performance liquid chromatography (HPLC), have become the most dominant [1,47,61,72,73,85,86,88,90,94,[122][123][124] for their more versatile use, reliability, reproducibility of results, possibility of automatization, identification of a large number of qualitative biomarkers in complex matrices, and low sample consumption [13,21,61,123,125]. coffee husks and stalks, cereals (e.g., chicory, corn, barley, wheat, rye, oats, rice, buckwheat, triticale, bran, and malt), legumes (e.g., soybeans, peas, chickpeas, and carob), roots (e.g., chicory or dandelion), vegetables (e.g., potatoes, carrots, and beetroots), fruits (e.g., figs, bananas, acai, and prunes), nuts (e.g., almonds, peanuts, and chestnuts), and seeds (especially cocoa and sunflower seeds). ...
... The method for analysing bamboo beverage free amino acids was adapted from Klikarova et al. (2021) with slight modifications. Five millilitres (5 mL) of fresh bamboo juice was mixed with 5 mL of extracting solvent (75% methanol in distilled water) and shaken for 2 min. ...
... Moreover, the present factor leads to considerable differentiation of phenolic and volatile compounds in EVOO [21]. This is proved in many previous studies mostly for the phenolic fraction [14,33,50,56]; in addition, according to Kiralan et al. [57], the volatile fraction (particularly (E)-2-hexenal) varied between oils from different provenance: significant dissimilarity was observed for monovarietal oils and different geographic regions (Hatay and Antalya that showed for instance a high variation in hexanal amount (29% and 14%, respectively), and explained by climatic conditions and location (latitude, longitude). ...
... Although there are many precedents demonstrating the usefulness of NMR techniques [13], qNMR is not routinely used to quantify sugars. From a practical point of view, quantitative phytochemical analysis in quality assurance is always limited by resources, including sample material, access to instrumentation, and investigation time. ...
... Taurine not present an oxidation peak in the assays performed. In fact, taurine does not exhibit a significant electroactivity and derivatization steps are necessary to produce an electrochemically active species to enable electroanalysis to be performed [52]. These results indicate that the proposed N,S-GQD-CS/GCE had good selectivity toward VB 2 , VB 6 and VB 12 in energy drink samples containing B-group vitamins. ...