Jipeng Wen’s research while affiliated with First Affiliated Hospital of China Medical University and other places

Sevoflurane (Sev) is a widely applied anesthetic in clinical practice; however, it could induce neurotoxicity and lead to postoperative cognitive dysfunction (POCD). This study aimed to investigate the role and underlying mechanism of circHOMER1 in Sev‐induced neurotoxicity and POCD. Sev treated mouse hippocampal neuronal HT22 cells and SD rats. RT‐qPCR was used to detect the levels of circHOMER1 and miR‐217. ELISA was employed to measure the levels of inflammatory factors IL‐6, IL‐1β, and TNF‐α. Commercially available kits assessed the concentration of MDA and measured the activities LDH and SOD. The CCK‐8 assay assessed cell viability. Flow cytometry analyzed cell apoptosis. The Morris water maze test evaluated the learning and cognitive abilities of the rats. Dual luciferase reporter assays and RIP experiments validated the targeted binding of circHOMER1 to miR‐217. Sev treatment significantly reduces cell viability, increases apoptosis, stimulates inflammatory responses and oxidative stress, and induces learning and memory impairments in SD rats. Following exposure to Sev, the expression of circHOMER1 is markedly decreased, while overexpression of circHOMER1 can alleviate Sev‐induced neuroinflammation, oxidative stress, and learning and memory deficits in rats. CircHOMER1 targets miR‐217, and transfection of miR‐217 antagonizes the neuroprotective effects of circHOMER1. This study demonstrated that circHOMER1 negatively regulated miR‐217, thereby inhibiting Sev‐induced neurotoxicity and learning and memory disorders.

Objective Tracheal intubation-related complications, such as postoperative sore throat (POST), hoarseness, and vocal cords injuries, are not uncommon. It is well known that thermal softening of double-lumen endobrochial tubes (DLTs) have been used to prevent these events from happeningn in non-smokers, however, no study has ever assessed the effects of thermal softening of DLTs in smokers undergoing one-lung anesthesia. We aim to investigate whether thermal softening of DLT can achieve a better effect in preventing POST. Design A randomized controlled trial. Setting A university-affiliated tertiary care centers. Participants A total of 258 smokers scheduled for one lung anesthesia were randomly assigned to one of the two groups. Interventions Group C (non-theromal softening group), group T (DLTs were placed in the 40°C0.9% saline for 10 minutes). Measurements and Main Results Incidence and severity of POST and hoarseness were assessed until 48 hours after surgery. Vocal cords were examined using laryngoscope before intubation and immediately after extubation. Patients’ hemodynamic change at intubation and extubation was recorded. The primary outcomes were the incidence and severity of POST. The secondary outcomes were the incidence and severity of hoarseness, vocal cords injuries and patients’ hemodynamic change at intubation and extubation.Sore throat and vocal cord injuries occurred less frequently in the thermal softening group than in the control group [31/129 vs 60/129, p<0.01; 21/129 vs 49/129, p<0.001; 12/129 vs 35/129, p<0.001 for sore throat; 14/70 vs 27/70, risk ratio (95% CI): 0.52 (0.30–0.90), P=0.025 for sore throat; 5/129 vs 52/129, p<0.05 for vocal cord injuries]. Conclusion Thermal softening of DLTs significantly reduced the incidence and severity of DLTs intubation-related POST within 72 hours after extubation.